Circulating lipocalin-2 across the adult lifespan DOI Creative Commons
Carlie Bauer, Cassandra Smith, Sara Vogrin

et al.

JBMR Plus, Journal Year: 2024, Volume and Issue: 9(2)

Published: Dec. 10, 2024

Lipocalin-2 (LCN2), a hormone produced by adipocytes, osteoblasts, and renal tubular cells, is implicated in age-related diseases, including cardio-metabolic disease. To understand the role LCN2 may play pathological states, we first need to elucidate relationship between circulating with indices of health during "normal" aging. This study examined serum levels LCN2, age, measures across adult lifespan males females. We conducted pooled cohort analysis 124 community-dwelling (n = 52) females 72) (age 20-87 yr, median BMI 25.92 [23.04, 29.81] kg/m2). Serum was analyzed using two-step chemiluminescent microparticle monoclonal immunoassay. The age evaluated linear regression cubic spline. Simple regressions were performed investigate following variables: BMI, VO2peak, glucose, body composition (DXA). For every 1 yr increase 0.26 mg/L higher (P .007, 95% CI [0.07, 0.45]). Each unit (kg/m2) associated 0.88 .027, [0.10, 1.66]) each VO2peak (mL/kg/min) 0.38 lower (p .003, [-0.63, -0.13]).There no significant sex, glucose or (all p > .05). increased linearly while it decreased as fitness level increased. Future research should build on these findings determine whether can be used biomarker for chronic disease if exercise mitigate changes.

Language: Английский

Sex-specific mechanisms in vascular aging: exploring cellular and molecular pathways in the pathogenesis of age-related cardiovascular and cerebrovascular diseases DOI Creative Commons
Anna Ungvari, Rafał Gulej, Roland Patai

et al.

GeroScience, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Language: Английский

Citations

3
Exercise-induced BDNF promotes PPARδ-dependent reprogramming of lipid metabolism in skeletal muscle during exercise recovery DOI
Wing Suen Chan, Chun Fai Ng, Brian Pak Shing Pang

et al.

Science Signaling, Journal Year: 2024, Volume and Issue: 17(828)

Published: March 19, 2024

Post-exercise recovery is essential to resolve metabolic perturbations and promote long-term cellular remodeling in response exercise. Here, we report that muscle-generated brain-derived neurotrophic factor (BDNF) elicits post-exercise reprogramming skeletal muscle. BDNF increased the expression of gene encoding PPARδ (peroxisome proliferator–activated receptor δ), a transcription master regulator lipid metabolism. After exercise, mice with muscle-specific Bdnf knockout ( MBKO ) exhibited impairments PPARδ-regulated expression, decreased intramuscular content, reduced β-oxidation, dysregulated mitochondrial dynamics. Moreover, required longer period recover from bout exercise did not show increases exercise-induced endurance capacity. Feeding naïve bioavailable mimetic 7,8-dihydroxyflavone resulted effects mimicked adaptations, including improved Together, our findings reveal an myokine for

Language: Английский

Citations

11
Methylome–proteome integration after late‐life voluntary exercise training reveals regulation and target information for improved skeletal muscle health DOI Creative Commons
Toby L. Chambers, Andrea Dimet‐Wiley, Alexander R. Keeble

et al.

The Journal of Physiology, Journal Year: 2024, Volume and Issue: 603(1), P. 211 - 237

Published: July 26, 2024

Exercise is a potent stimulus for combatting skeletal muscle ageing. To study the effects of exercise on in preclinical setting, we developed combined endurance-resistance training mice called progressive weighted wheel running (PoWeR). PoWeR improves molecular, biochemical, cellular and functional characteristics promotes aspects partial epigenetic reprogramming when performed late life (22-24 months age). In this investigation, leveraged pan-mammalian DNA methylome arrays tandem mass-spectrometry proteomics to provide detailed information late-life adaptations female relative age-matched sedentary controls (n = 7-10 per group). Differential CpG methylation at conserved promoter sites was related transcriptional regulation genes as well Nr4a3, Hes1 Hox after PoWeR. Using holistic method -omics integration binding expression target analysis (BETA), changes were associated with upregulated proteins global mitochondrial translation (P 0.03). Specifically, BETA implicated control ribosomal, mitoribosomal, complex I protein abundance training. may also influence LACTB, MIB1 UBR4 induction - all are mechanistically linked health. Computational cistrome predicted several transcription factors including MYC regulators trained methylome-proteome landscape, corroborating prior transcriptome data. Correlating proteome mass fatigue resistance revealed positive relationships VPS13A NPL levels, respectively. Our findings expose differential proteomic translational that could function aged mice. KEY POINTS: Late-life from 22-24 age shown improve vivo promote mitigation. Integration 36k using (which contain ageing clock sites) exploratory extends our work reveals coordinated widespread initiation, ribosomal (mitoribosomal) voluntary sizeable cohort group analysis). Multi-omics serine β-lactamase-like (LACTB tumour muscle), mind bomb 1 (MIB1 satellite cell type 2 fibre maintenance) ubiquitin ligase E3 component N-recognin 4 (UBR4 quality control) identified regulator proteome, agreement analyses. Vacuolar sorting 13 homolog A (VPS13A) positively correlated mass, glycoprotein/glycolipid sialylation enzyme N-acetylneuraminate pyruvate lyase (NPL) resistance.

Language: Английский

Citations

6
Map of epigenetic age acceleration: a worldwide analysis DOI
Igor Yusipov, Alena Kalyakulina,

Arseniy Trukhanov

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 100, P. 102418 - 102418

Published: July 14, 2024

Language: Английский

Citations

5
Molecular Effects of Physical Activity and Body Composition: A Systematic Review and Meta-Analysis DOI Open Access
Jenni Chambers, Clare M. P. Roscoe, Corinna Chidley

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2025, Volume and Issue: 22(4), P. 637 - 637

Published: April 18, 2025

Physical activity (PA) and body composition are important lifestyle factors that influence public health. Research suggests DNA regions (CpG site locations) differentially methylated in a physically active population. This meta-analysis aimed to identify CpG sites associated with various levels of PA metabolic pathways. The followed PRISMA guidelines using PubMed, SportDISCUS, Embase, Scopus, Cochrane Web Science. Epigenomic analyses performed on participants no underlying health conditions were included. Articles screened Rayyan AI extracted sites, their location confirmed the EWAS catalogue. Six studies comprising 770 subjects included this meta-analysis. was clinical metrics from six showed BMI, blood pressure, insulin glucose testing significantly improved upon intervention. Amongst studies, total 257 participants, 134 CpGs located 92 genes obesity-related identified either belonged lipid metabolism or signalling pathway. which regulated multiple tissue types JAZF1 (insulin signalling, carbohydrate pathways) NAV1 (mTOR pathway). In conclusion, current epigenomic induce differential methylation signatures affect metabolism. To understand positive molecular effects PA, further research above candidate needs be conducted amongst

Language: Английский

Citations

0
Sirt2 deficiency aggravates intramuscular adipose tissue infiltration and impairs myogenesis with aging in male mice DOI
Eunjoo H. Lee, Sunyoung Park, Kyu‐Shik Jeong

et al.

Biogerontology, Journal Year: 2025, Volume and Issue: 26(3)

Published: April 21, 2025

Language: Английский

Citations

0
Epigenetic and “redoxogenetic” adaptation to physical exercise DOI Creative Commons
Zsolt Radák, Lang Pan, Lei Zhou

et al.

Free Radical Biology and Medicine, Journal Year: 2023, Volume and Issue: 210, P. 65 - 74

Published: Nov. 17, 2023

Language: Английский

Citations

10
Map of epigenetic age acceleration: a worldwide meta-analysis DOI Creative Commons
Igor Yusipov, Alena Kalyakulina, Claudio Franceschi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 17, 2024

Abstract This study is the first systematic meta-analysis of epigenetic age acceleration largest publicly available DNA methylation data for healthy samples (93 datasets, 23K samples), focusing on geographic and ethnic aspects different countries (25 countries) populations (31 ethnicities) around world. The most popular tools assessing were examined in detail, their quality metrics analyzed, ability to extrapolate from tissue types ranges training these models was explored. In cases, are not consistent with each other show signs acceleration, PhenoAge model tending systematically underestimate versions GrimAge overestimate prediction subjects. Although GEO open-access database, represented, datasets use criteria determining controls. Because this, it difficult fully isolate contribution “geography/environment”, “ethnicity” “healthiness” acceleration. However, DunedinPACE metric, which measures aging rate, adequately reflects standard living socioeconomic indicators countries, although can be applied only blood data. When comparing males faster than females considered.

Language: Английский

Citations

3
Remodeling of the human skeletal muscle proteome found after long-term endurance training but not after strength training DOI Creative Commons
Eric B. Emanuelsson, Muhammad Arif, Stefan Markus Reitzner

et al.

iScience, Journal Year: 2023, Volume and Issue: 27(1), P. 108638 - 108638

Published: Dec. 5, 2023

Exercise training has tremendous systemic tissue-specific health benefits, but the molecular adaptations to long-term exercise are not completely understood. We investigated skeletal muscle proteome of highly endurance-trained, strength-trained, and untrained individuals performed exercise- sex-specific analyses. Of 6,000+ proteins identified, >650 were differentially expressed in endurance-trained compared with controls. Strikingly, 92% shared higher expression both male female endurance groups known mitochondrial. In contrast findings individuals, minimal differences found strength-trained between females males. Lastly, a co-expression network comparative literature analysis revealed key pathways related benefits exercise, which primarily mitochondrial proteins. This is available as an interactive database resource where investigators can correlate clinical data global gene protein for hypothesis generation.

Language: Английский

Citations

8
Effects of chronic alcohol intoxication on aerobic exercise-induced adaptations in female mice DOI
Abigail L. Tice, Bradley S. Gordon, Emma Fletcher

et al.

Journal of Applied Physiology, Journal Year: 2024, Volume and Issue: 136(4), P. 721 - 738

Published: Feb. 15, 2024

Chronic alcohol intoxication decreases muscle strength/function and causes mitochondrial dysfunction. Aerobic exercise training improves oxidative capacity increases mass strength. Presently, the impact of chronic on aerobic exercise-induced adaptations was investigated. Female C57BL/6Hsd mice were randomly assigned to one four groups: control sedentary (CON SED;

Language: Английский

Citations

2