Punicalagin Attenuates LPS-Induced Inflammation and ROS Production in Microglia by Inhibiting the MAPK/NF-κB Signaling Pathway and NLRP3 Inflammasome Activation

Journal of Inflammation Research, Journal Year: 2022, Volume and Issue: Volume 15, P. 5347 - 5359

Published: Sept. 1, 2022

Purpose: Neurodegenerative diseases are associated with neuroinflammation along activation of microglia and oxidative stress, but currently lack effective treatments. Punicalagin is a natural bio-sourced product that exhibits anti-inflammatory effects on several chronic diseases; however, the anti-oxidative have not been well examined. This study aimed to investigate punicalagin LPS-induced inflammatory responses, NLRP3 inflammasome activation, production ROS using murine BV2 cells. Methods: cells were pre-treated following LPS treatment induce inflammation. The secretion NO PGE 2 was analyzed by Griess reagent ELISA respectively, while expressions iNOS, COX-2, STAT3, ERK, JNK, p38 Western blotting, IL-6 measured ELISA, activity NF-κB detected promoter reporter assay. To examine whether affects stimulated then treated ATP or nigericin. IL-1β ELISA. inflammasome-related proteins phospho IκBα/IκBα blotting. intracellular mitochondrial flow cytometry. Results: Our results showed attenuated inflammation reduction pro-inflammatory mediators cytokines including IL-1β, led inhibition STAT3 phosphorylation also suppressed phosphorylation, activity, inhibited inflammasome, reduced Conclusion: demonstrated through suppressing expression iNOS MAPK/NF-κB signaling pathway in microglia, suggesting might potential treating neurodegenerative diseases. Graphical Abstract: Keywords: punicalagin, MAPK,

Language: Английский

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Mitochondria-targeted fluorescent probe for imaging viscosity in hepatic ischemia–reperfusion injury cell model

Chemical Communications, Journal Year: 2022, Volume and Issue: 59(8), P. 1030 - 1033

Published: Dec. 21, 2022

The viscosity of the cell microenvironment is a parameter that affects physiological processes. A fluorescent probe X-V was designed to detect changes hepatic ischemia-reperfusion injury (HIRI) model with high selectivity and sensitivity. fluorescence emission wavelength 615 nm Stokes shift can be up 125 nm, which used not only for intracellular stimulated by different drugs but also detection in HIRI model. Probe provides useful tool study relationship between mitochondrial related diseases.

Language: Английский

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Modulating miR-146a Expression by Hydrogen Sulfide Ameliorates Motor Dysfunction and Axonal Demyelination in Cuprizone-Induced Multiple Sclerosis

ACS Chemical Neuroscience, Journal Year: 2023, Volume and Issue: 14(17), P. 3047 - 3058

Published: Aug. 16, 2023

Multiple sclerosis (MS) is a progressive neuro-inflammatory and neuro-autoimmune disease. Although hydrogen sulfide has recently shown potential therapeutic impacts in different neurological diseases, its effects on MS are still obscure. MiR-146a considered vital target for approaches treating MS. The present study directed to explore the of NaHS (hydrogen donor) cuprizone-induced whether can mediate via regulating miR-146a expression. A total 28 male C57Bl/6 mice were divided into 4 groups; control, cuprizone-intoxicated, control (100 μmol/kg/day, i.p), NaHS-treated groups. Intriguingly, treatment managed improve locomotor coordination curb neuronal inflammation demyelination as evidenced by hematoxylin & eosin, Luxol fast blue staining increased myelin basic protein (MBP) content. Additionally, reduced interleukin-1 receptor-associated kinase-1 (IRAK-1), nuclear transcription factor kappa B (NF-κB), interleukin (IL)-17, IL-1β brain levels along with downregulation expression compared untreated cuprizone-intoxicated group. Furthermore, animals revealed much less oxidative stress elevated glutathione malondialdehyde contents. Altogether, current work reported that could motor dysfunction reduce axonal demyelination, stress, well neuro-inflammation Thus, using H2S-releasing compounds be promising approach strategies. mechanism these beneficial may involve regulation miR-146a/NF-κB/IL-1β axis.

Language: Английский

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Mechanistic Intimate Insights into the Role of Hydrogen Sulfide in Alzheimer’s Disease: A Recent Systematic Review

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(20), P. 15481 - 15481

Published: Oct. 23, 2023

In the rapidly evolving field of Alzheimer's Disease (AD) research, intricate role Hydrogen Sulfide (H

Language: Английский

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Neuroprotective Effects of Sodium Nitroprusside on CKD-Induced Cognitive Dysfunction in Rats: Role of CBS and Nrf2/HO-1 Pathway

NeuroMolecular Medicine, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 7, 2025

Language: Английский

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