The Natural History of Integrons DOI Creative Commons
Timothy M. Ghaly, Michael R. Gillings, Anahit Penesyan

et al.

Microorganisms, Journal Year: 2021, Volume and Issue: 9(11), P. 2212 - 2212

Published: Oct. 25, 2021

Integrons were first identified because of their central role in assembling and disseminating antibiotic resistance genes commensal pathogenic bacteria. However, these clinically relevant integrons represent only a small proportion integron diversity. are now known to be ancient genetic elements that hotspots for genomic diversity, helping generate adaptive phenotypes. This perspective examines the functions, activities within both natural clinical environments. We show how fundamental properties exquisitely pre-adapted them respond selection pressures imposed by human use antimicrobial compounds. then follow extraordinary increase abundance one class (class 1) has resulted from its acquisition multiple mobile elements, subsequent colonisation diverse bacterial species, wide range animal hosts. Consequently, this become significant pollutant own right, extent it can detected most ecosystems. As continue drive environmental instability, will likely play key roles adaptation settings. Understanding ecological evolutionary dynamics help us predict shape outcomes have direct relevance ecosystem health.

Language: Английский

Antibiotic resistance in the environment DOI Open Access
D. G. Joakim Larsson, Carl‐Fredrik Flach

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 20(5), P. 257 - 269

Published: Nov. 4, 2021

Language: Английский

Citations

1738
Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered DOI Creative Commons
Mohan Amarasiri, Daisuke Sano, Satoru Suzuki

et al.

Critical Reviews in Environmental Science and Technology, Journal Year: 2019, Volume and Issue: 50(19), P. 2016 - 2059

Published: Dec. 3, 2019

Aquatic environments are identified as an ideal setting for acquisition and dissemination of antibiotic resistance, human exposure to resistant bacteria (ARB) resistance genes (ARGs) in aquatic may pose additional health risk. Quantitative microbial risk assessment (QMRA) has been suggested a suitable method evaluate quantify this However, information about the ARB ARGs is lacking many scenarios dose-response models regarding infections not developed yet. This review summarizes current knowledge highlights challenging questions remaining be answered better forecast risks caused by water environments. The include what missing needed exposing environments? markers ARB/ARGs contamination how frequently do ARG selection propagation occur there any unknown hot spots? Studies on above topics will contribute management its health.Abbreviations3GC3rd generation cephalosporinsARBAntibiotic bacteriaARGAntibiotic geneCFUColony forming unitDBPDisinfection by-productseDNAExtracellular DNAEPSExtracellular polymeric substancesHGTHorizontal gene transferISCRInsertion sequence common regionMARMultiple resistantMICMinimum inhibitory concentrationMGEMobile genetic elementsMSWMunicipal solid wasteQMRAQuantitative assessmentVBNCViable but non-culturableWWTPWastewater treatment plant

Language: Английский

Citations

497
Antibiotic resistance in wastewater treatment plants: Tackling the black box DOI
Célia M. Manaia, Jaqueline Rocha, Nazareno Scaccia

et al.

Environment International, Journal Year: 2018, Volume and Issue: 115, P. 312 - 324

Published: April 6, 2018

Language: Английский

Citations

412
Sulfonamide drugs: structure, antibacterial property, toxicity, and biophysical interactions DOI

Aben Ovung,

Jhimli Bhattacharyya

Biophysical Reviews, Journal Year: 2021, Volume and Issue: 13(2), P. 259 - 272

Published: March 29, 2021

Language: Английский

Citations

338
Critical knowledge gaps and research needs related to the environmental dimensions of antibiotic resistance DOI Creative Commons
D. G. Joakim Larsson,

Antoine Andremont,

Johan Bengtsson‐Palme

et al.

Environment International, Journal Year: 2018, Volume and Issue: 117, P. 132 - 138

Published: May 7, 2018

There is growing understanding that the environment plays an important role both in transmission of antibiotic resistant pathogens and their evolution. Accordingly, researchers stakeholders world-wide seek to further explore mechanisms drivers involved, quantify risks identify suitable interventions. a clear value establishing research needs coordinating efforts within across nations order best tackle this global challenge. At international workshop late September 2017, scientists from 14 countries with expertise on environmental dimensions resistance gathered define critical knowledge gaps. Four key areas were identified where urgently needed: 1) relative contributions different sources antibiotics bacteria into environment; 2) environment, particularly anthropogenic inputs, evolution resistance; 3) overall human animal health impacts caused by exposure bacteria; 4) efficacy feasibility technological, social, economic behavioral interventions mitigate resistance.1.

Language: Английский

Citations

336
A conceptual framework for the environmental surveillance of antibiotics and antibiotic resistance DOI Creative Commons

P.M.C. Huijbers,

Carl‐Fredrik Flach, D. G. Joakim Larsson

et al.

Environment International, Journal Year: 2019, Volume and Issue: 130, P. 104880 - 104880

Published: June 17, 2019

Environmental surveillance of antibiotics and antibiotic resistance could contribute toward the protection human, animal ecosystem health. However, justification for choice markers sampling sites that informs about different risk scenarios is often lacking. Here, we define five fundamentally objectives in environment. The first objective (1) to address transmission already antibiotic-resistant bacteria humans via environmental routes. second (2) accelerating evolution pathogens through pollution with selective agents human or origin. third (3) risks pose aquatic terrestrial health, including effects on functions services. two final overlap those traditional clinical surveillance, namely, identify (4) population-level prevalence (5) use. latter have particular potential countries where data consumption are scarce absent. For each objective, levels evidence provided by phenotypic genotypic microbial markers, as well residues, discussed evaluated a conceptual level. Furthermore, monitoring would be particularly informative identified. proposed framework one starting points guiding various spatiotemporal scales, harmonizing such activities existing systems.

Language: Английский

Citations

189
Biodegradation of antibiotics: The new resistance determinants – part I DOI
Ana C. Reis, Boris A. Kolvenbach, Olga C. Nunes

et al.

New Biotechnology, Journal Year: 2019, Volume and Issue: 54, P. 34 - 51

Published: Aug. 11, 2019

Language: Английский

Citations

149
Deciphering the transfers of antibiotic resistance genes under antibiotic exposure conditions: Driven by functional modules and bacterial community DOI
Qi Zhao, Wanqian Guo,

Haichao Luo

et al.

Water Research, Journal Year: 2021, Volume and Issue: 205, P. 117672 - 117672

Published: Sept. 17, 2021

Language: Английский

Citations

138
Antimicrobials and Antibiotic Resistance Genes in Water Bodies: Pollution, Risk, and Control DOI Creative Commons
B. Singh, Rajinder Kaur, Shashikala Verma

et al.

Frontiers in Environmental Science, Journal Year: 2022, Volume and Issue: 10

Published: April 28, 2022

The manuscript endeavors to provide a perspective on the role of water bodies in spread antimicrobial (antibiotic) resistance (AMR), resistant bacteria (ARB), and genes (ARGs) among pathogens, animals, humans. We briefly indicate how AMR problem is globally affecting public health, along with strategies mechanisms combat dissemination ARB ARGs. A brief systematic survey literature (2015-onwards) for presence residues occurrence ARGs microorganisms different bodies/sources indicates gravity situation suggests their important AMR, ARB, prevalent treatment methods which tend reduce from resources are unable remove them completely, allowing continue organisms concern. In this opinion article, we attempt underline key controlling release/discharge contaminants buildup checking development AMR. reduction release antibiotic environment, especially bodies, combined improved surveillance means efficacious treatment/removal/decomposition could help curb menace effectively. suggest expansion ambit ‘One Health Approach crises proposed by World Bank, 2021 include ‘reduction contamination environment’ as ‘seventh domain’ activity effectively achieve its objective.

Language: Английский

Citations

73
Molecular mechanism of plasmid-borne resistance to sulfonamide antibiotics DOI Creative Commons
Meenakshi Venkatesan,

Michael Fruci,

Lou Ann Verellen

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 7, 2023

Abstract The sulfonamides (sulfas) are the oldest class of antibacterial drugs and inhibit bacterial dihydropteroate synthase (DHPS, encoded by folP ), through chemical mimicry its co-substrate p -aminobenzoic acid ( ABA). Resistance to sulfa is mediated either mutations in or acquisition sul genes, which code for sulfa-insensitive, divergent DHPS enzymes. While molecular basis resistance well understood, mechanisms mediating -based have not been investigated detail. Here, we determine crystal structures most common Sul enzyme types (Sul1, Sul2 Sul3) multiple ligand-bound states, revealing a substantial reorganization their ABA-interaction region relative corresponding DHPS. We use biochemical biophysical assays, mutational analysis, trans complementation E. coli Δ show that Phe-Gly sequence enables enzymes discriminate against sulfas while retaining ABA binding necessary broad sulfonamides. Experimental evolution results strain harboring sulfa-resistant variant carries insertion active site, recapitulating this mechanism. also possess increased site conformational dynamics DHPS, could contribute substrate discrimination. Our reveal foundation Sul-mediated drug facilitate potential development new less prone resistance.

Language: Английский

Citations

42