Vaginal microbiota transplantation is a truly opulent and promising edge: fully grasp its potential

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: March 22, 2024

Vaginal microbiota transplantation (VMT) is a cutting-edge treatment modality that has the potential to revolutionize management of vaginal disorders. The human vagina complex and dynamic ecosystem home diverse community microorganisms. These microorganisms play crucial role in maintaining health well-being female reproductive system. However, when balance this disrupted, it can lead development various Conventional treatments, such as antibiotics antifungal medications, temporarily relieve symptoms they often fail address underlying cause problem, which disruption microbiota. In recent years, VMT emerged promising therapeutic approach aims restore ecosystem. Several studies have demonstrated safety efficacy treating bacterial vaginosis, recurrent yeast infections, other conditions. procedure also shown results reducing risk sexually transmitted infections preterm birth pregnant women. more research needed establish optimal donor selection, preparation, screening protocols, well long-term efficacy. offers safe, effective, minimally invasive option for women with persistent problems. It could improve quality life millions worldwide become standard shortly. With further development, potentially treat wide range problems beyond scope

Language: Английский

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Vaginal microbiomes show ethnic evolutionary dynamics and positive selection of Lactobacillus adhesins driven by a long-term niche-specific process

Cell Reports, Journal Year: 2024, Volume and Issue: 43(4), P. 114078 - 114078

Published: April 1, 2024

The vaginal microbiome's composition varies among ethnicities. However, the evolutionary landscape of microbiome in multi-ethnic context remains understudied. We perform a systematic analysis 351 samples from 35 pregnant women, addition to two validation cohorts, totaling 462 90 women. Microbiome alpha diversity and community state dynamics show strong ethnic signatures. Lactobacillaceae have higher ratio non-synonymous synonymous polymorphism lower nucleotide than non-Lactobacillaceae all ethnicities, with large repertoire positively selected genes, including mucin-binding cell wall anchor genes. These are driven by long-term process unique human niche. Finally, we propose an model reflecting environmental niches microbes. Our study reveals extensive signatures microbial ecology evolution, highlighting importance studying host-microbiome ecosystem perspective.

Language: Английский

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Defining Vaginal Community Dynamics: daily microbiome transitions, the role of menstruation, bacteriophages, and bacterial genes

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Aug. 19, 2024

The composition of the vaginal microbiota during menstrual cycle is dynamic, with some women remaining eu- or dysbiotic and others transitioning between these states. What defines dynamics, whether differences are microbiome-intrinsic mostly driven by host unknown. To address this, we characterized 49 healthy, young metagenomic sequencing daily swabs a cycle. We classified dynamics microbiome assessed impact behavior as well at species, strain, gene, phage levels.

Language: Английский

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The human vaginal microbiota: from clinical medicine to models to mechanisms

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 77, P. 102422 - 102422

Published: Jan. 11, 2024

Language: Английский

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VIRGO2: Unveiling the Functional and Ecological Complexity of the Vaginal Microbiome with an Enhanced Non-Redundant Gene Catalog

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Despite the importance of cervicovaginal microbiome, mechanisms that govern its composition and drive impact on host physiology remain poorly understood. This study expands our understanding function ecology vaginal microbiome using VIRGO2, an enhanced non-redundant gene catalog comprising over 1.7 million well-annotated genes from body-site specific microbes viruses. Analyses VIRGO2 revealed novel insights, including identification previously uncharacterized bacteria, features mycobiome phageome, differential expression bacterial carbohydrate catabolic genes. Constructed 2,500 metagenomes 4,000 genomes, broadens geographic representation microbial diversity compared to predecessor. updated enables more precise profiling taxonomic functional metagenomic metatranscriptomic datasets. is a critical resource for integrative analyses communities their interactions with tissues, thereby enhancing mechanistic health disease.

Language: Английский

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Factors shaping vaginal microbiota long-term community dynamics in young adult women

Peer Community Journal, Journal Year: 2025, Volume and Issue: 5

Published: March 12, 2025

The vaginal microbiota is known to affect women's health. Yet, there a notable paucity of high-resolution follow-up studies lasting several months, which would be required interrogate the long-term dynamics and associations with demographic behavioural covariates. Here, we present longitudinal cohort study 125 women, followed for median duration 8.6 11 samples collected per woman. Using hierarchical Bayesian Markov model, characterised patterns community persistence transition, simultaneously estimated impact 16 covariates quantified individual variability among women. We showed that "optimal" (Community State Type (CST) I, II, V) "sub-optimal" (CST III) communities are more stable over time than "non-optimal" IV) ones. Furthermore, found some — most notably alcohol consumption impacted probability shifting from one CST another. performed counterfactual simulations confirm alterations key covariates, such as consumption, could shape prevalence different in population. Finally, our analyses indicated relatively canalised pathway leading deterioration communities, whereas paths recovery can highly individualised In addition providing first insights into year, showcases novel application model clinical data many Our findings pave way an improved mechanistic understanding microbial environment development preventative therapeutic strategies improve

Language: Английский

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Host-vaginal microbiota interaction: shaping the vaginal microenvironment and bacterial vaginosis

Current Clinical Microbiology Reports, Journal Year: 2024, Volume and Issue: 11(3), P. 177 - 191

Published: April 19, 2024

Abstract Purpose of review This comprehensively examines host-vaginal microbiota interactions, the composition vaginal microbiota, and its dynamic changes throughout a woman's lifespan. Furthermore, intricate interplay between host beneficial bacterial communities, such as Lactobacillus species, opportunistic pathogens, Gardnerella spp ., associated with BV development, has been explored. Additionally, current advanced therapeutic strategies for managing complications related to along challenges faced in this field have discussed. Recent findings shown that microbial communities inhabiting female vagina, known play critical role maintaining women's health supporting reproductive activities. Imbalanced can predispose individuals range diseases, including vaginosis (BV), sexually transmitted infections (STI), miscarriage, preterm birth. While exact mechanisms by which -dominated microenvironment improves remain elusive, gaining insight into interactions well help address unanswered questions. Summary A deeper understanding reciprocal potential pave way development novel diagnostic interventions improvement health.

Language: Английский

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The microbiome in recurrent pregnancy loss – A scoping review

Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 163, P. 104251 - 104251

Published: April 25, 2024

Recurrent pregnancy loss (RPL) is a troubling condition that affects couples worldwide. Despite extensive research efforts, many RPL cases remain unexplained, highlighting the need for novel approaches to unravel its underlying mechanisms. Recent advances in microbiome have shed light on potential role of reproductive health and outcomes. Based systematic literature research, this review aims comprehensively explore current understanding microbiome's involvement RPL, focusing vaginal, endometrial, gut microbiomes. Evidence from available studies examined explain relationship between RPL. Furthermore, we discuss diagnostic microbiome, therapeutic interventions, future directions Understanding complex interactions holds promise developing targeted interventions help patients today diagnosed as unexplained.

Language: Английский

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Comparison of the lower genital tract microbiome composition in patients with benign gynecological disease

Frontiers in Global Women s Health, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 21, 2025

Objective Lower genital tract microbiome dysbiosis has been associated with several gynecological diseases. However, the differences in composition among patients diseases, such as endometrial polyps and uterine myoma, are poorly understood. Studying lower benign diseases could provide new insights for interpreting complex interplay between pathogenesis finding targets preventive measures. Methods A total of 16 (EPs), 11 myoma (UM), 6 ovarian cysts (OC) 36 healthy women (HWs) were recruited this study. Samples obtained from vaginal secretions. The DNA was isolated samples, V3-V4 regions amplified. sequencing libraries generated sequenced on an Illumina NovaSeq 6000 platform. Results Firmicutes, Actinobacteria Bacteroidota most common phyla all four groups, whereas OC presented highest abundance Firmicutes lowest Bacteroidota. At genus level, Lactobacillus group significantly greater than that HW group, Atopobium UM group. Gardnerella EP Streptococcus richness evenness generally consistent HW, EP, UM, groups. Principal component analysis (PCA), principal coordinate (PCoA) nonmetric multidimensional scaling (NMDS) revealed no distinct separation trends According to ANOSIM, there significant difference community structure Conclusions nonsignificant result diversity comparison different we demonstrated had a Atopobium, which might contribute occurrence providing clues

Language: Английский

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Vaginal Microbiome and Fertility

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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