Hydrogel-Based Scaffolds: Advancing Bone Regeneration Through Tissue Engineering DOI Creative Commons

Jalima Quintero,

Rosalío Ramos‐Payán, José Geovanni Romero-Quintana

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(3), P. 175 - 175

Published: Feb. 27, 2025

Bone tissue engineering has emerged as a promising approach to addressing the limitations of traditional bone grafts for repairing defects. This regenerative medicine strategy leverages biomaterials, growth factors, and cells create favorable environment regeneration, mimicking body’s natural healing process. Among various biomaterials explored, hydrogels (HGs), class three-dimensional, hydrophilic polymer networks, have gained significant attention scaffolds engineering. Thus, this review aimed investigate potential synthetic HGs, molecules used its functionalization, enhanced applications. HGs offer several advantages such scaffolds, including biocompatibility, biodegradability, tunable mechanical properties, ability encapsulate deliver bioactive molecules. These properties make them ideal candidates supporting cell attachment, proliferation, differentiation, ultimately guiding formation new tissue. The design optimization HG-based involve adapting their composition, structure, meet specific requirements regeneration. Current research focuses on incorporating molecules, factors cytokines, into HG further enhance osteoinductive osteoconductive properties. Additionally, strategies improve strength degradation kinetics are being explored ensure long-term stability support formation. development advanced holds great revolutionizing providing effective treatment options patients with

Language: Английский

A Multifunctional Nanocomposite Hydrogel Delivery System Based on Dual‐Loaded Liposomes for Scarless Wound Healing DOI
Danlei Xing,

Guoqing Xia,

Xu-Dong Tang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(28)

Published: July 16, 2024

Abstract Increased inflammatory responses and oxidative stress at the wound site following skin trauma impair healing. Furthermore, scarring places fibroblasts under severe mechanical aggravates pathological fibrosis. A novel liposomal composite hydrogel is engineered for microenvironment remodeling, incorporating dual‐loaded liposomes into gelatin methacrylate to create a nanocomposite hydrogel. Notably, tetrahydrocurcumin (THC) hepatocyte growth factor (HGF) are encapsulated in hydrophobic hydrophilic layers of liposomes, respectively. The maintains porous nanoarchitecture, demonstrating sustainable THC HGF release enhanced properties biocompatibility. This system effectively promotes cell proliferation angiogenesis attenuates apoptosis. It decreases expression factors by inhibiting high‐mobility group box /receptor advanced glycation end product/NF‐κB (HMGB1/RAGE/NF‐κB)pathway increases macrophage polarization from M1 M2 vitro, controlling responses. exhibits remarkable antioxidant scavenging excess reactive oxygen species free radicals. Most importantly, it prevents scar formation restraining transforming beta (TGF‐β)/Smads pathway that downregulates associated fibrotic factors. demonstrates strong therapeutic effects against inflammation fibrosis rat model with biosafety, advancing development innovative hydrogel‐based delivery strategies clinical scarless therapy.

Language: Английский

Citations

6
Application of Artificial Intelligence at All Stages of Bone Tissue Engineering DOI Creative Commons
E. R. Kolomenskaya, Vera V. Butova, Artem Poltavskiy

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 12(1), P. 76 - 76

Published: Dec. 28, 2023

The development of artificial intelligence (AI) has revolutionized medical care in recent years and plays a vital role number areas, such as diagnostics forecasting. In this review, we discuss the most promising areas AI application to field bone tissue engineering prosthetics, which can drastically benefit from AI-assisted optimization patient personalization implants scaffolds ways ranging visualization real-time monitoring implantation cases prediction, thereby leveraging compromise between specific architecture decisions, material choice, synthesis procedure. With emphasized crucial accuracy robustness developed algorithms, especially engineering, it was shown that rigorous validation testing, demanding large datasets extensive clinical trials, are essential, how through developing multidisciplinary cooperation among biology, chemistry with materials science, AI, these challenges be addressed.

Language: Английский

Citations

13
A composite hydrogel loaded with the processed pyritum promotes bone repair via stimulate the osteogenic differentiation of BMSCs DOI

Xingyu Zhu,

Huanjin Liu,

Chunmei Mei

et al.

Biomaterials Advances, Journal Year: 2024, Volume and Issue: 160, P. 213848 - 213848

Published: April 2, 2024

Language: Английский

Citations

5
Functional extracellular vesicles from SHEDs combined with gelatin methacryloyl promote the odontogenic differentiation of DPSCs for pulp regeneration DOI Creative Commons
Hui Lü,

Qing Mu,

Weili Ku

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 17, 2024

Abstract Background Pulp regeneration is a novel approach for the treatment of immature permanent teeth with pulp necrosis. This technique includes combination stem cells, scaffolds, and growth factors. Recently, cell-derived extracellular vesicles (EVs) have emerged as new methodology regeneration. Emerging evidence has proven that preconditioning an effective scheme to modify EVs better therapeutic potency. Meanwhile, proper scaffolding great significance protect from rapid clearance destruction. investigation aims fabricate injectable hydrogel loaded pre-differentiated cells human exfoliated deciduous (SHEDs) examine their effects on Results We successfully employed odontogenic induction medium (OM) SHEDs generate functional EV (OM-EV). The OM-EV at concentration 20 µg/mL was demonstrated promote proliferation migration dental (DPSCs). results revealed potential differentiation DPSCs than common (CM-EV) in vitro through Alizarin red phalloidin, alkaline phosphatase staining, assessment expression odontogenic-related markers. High-throughput sequencing suggests superior may be attributed activation AMPK/mTOR pathway. Simultaneously, we prepared photocrosslinkable gelatin methacryloyl (GelMA) construct OM-EV-encapsulated hydrogel. exhibited sustained release good biocompatibility DPSCs. released could internalized by DPSCs, thereby enhancing survival migration. In tooth root slices were subcutaneously transplanted nude mice, found facilitate dentinogenesis. After 8 weeks, there more formation mineralized tissue, well higher levels dentin sialophosphoprotein (DSPP) matrix protein-1 (DMP-1). Conclusions can substantially enhanced SHEDs. combined GelMA are capable effectively promoting dentinogenesis upregulating which provides promising

Language: Английский

Citations

4
Tannic acid and quaternized chitosan mediated puerarin-loaded octacalcium phosphate /sodium alginate scaffold for bone tissue engineering DOI

Yan Chen,

Tengbin Shi, Lan Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 271, P. 132632 - 132632

Published: May 24, 2024

Language: Английский

Citations

4
Advances in the design, preparation and application of biomimetic damping materials DOI Creative Commons
Xuefan Gu, Ling Wang, Xin Guan

et al.

Giant, Journal Year: 2024, Volume and Issue: 19, P. 100321 - 100321

Published: July 11, 2024

Biomimetic damping materials have emerged as promising candidates for various applications due to their ability mimic the exceptional properties observed in biological systems. This review provides a comprehensive overview of recent advances field biomimetic gel materials. The conceptual framework is discussed, synthesis methods inspired by principles are elucidated, and key considerations material selection highlighted. latest research findings on mechanical properties, biocompatibility practical these synthesized insights into future directions offered.

Language: Английский

Citations

4
Gelatin Methacryloyl (GelMA) - 45S5 Bioactive Glass (BG) Composites for Bone Tissue Engineering: 3D Extrusion Printability and Cytocompatibility Assessment Using Human Osteoblasts DOI
Memoona Akhtar, Peixi Peng, Anne Bernhardt

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(8), P. 5122 - 5135

Published: July 22, 2024

3D extrusion printing has been widely investigated for low-volume production of complex-shaped scaffolds tissue regeneration. Gelatin methacryloyl (GelMA) is used as a baseline material the synthesis biomaterial inks, often with organic/inorganic fillers, to obtain balance between good printability and biophysical properties. The present study demonstrates how 45S5 bioactive glass (BG) addition GelMA concentrations can be tailored develop composite buildability. experimental results suggest that BG consistently decreases compression stiffness, irrespective concentration, albeit within 20% scaffold (without BG). optimal 2 wt % in 7.5 was demonstrated provide best combination buildability route. degradation swelling kinetics increases addition, concentration. Importantly, dissolution simulated body fluid over 3 weeks clearly promoted nucleation growth crystalline calcium phosphate particles, indicating potential GelMA-45S5 promote biomineralization. cytocompatibility assessment using human osteoblasts could demonstrate uncompromised cell proliferation or osteogenic marker expression 21 days culture printable -2 when compared GelMA. thus encourage further investigations GelMA/45S5 system bone engineering applications.

Language: Английский

Citations

4
Application of Mg-MOF-loaded gelatin microspheres with osteogenic, angiogenic, and ROS scavenging capabilities in bone defect repair DOI

Zhiheng Luo,

Jianxiong Ma, Yan Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 135721 - 135721

Published: Sept. 1, 2024

Language: Английский

Citations

4
Dynamic culturing of large cell-loaded PCL/gelatin methacryloyl scaffolds for bone critical size defect repair DOI

Guangxin Shen,

Botao Gao, Jiayi Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 298, P. 139906 - 139906

Published: Jan. 17, 2025

Language: Английский

Citations

0
GelMA Hydrogels Integrated With aptamer CH6‐Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration DOI Open Access
Shebin Hong,

Ya Cui,

Dongming He

et al.

Macromolecular Bioscience, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Osteoporotic bone regeneration is challenging due to impaired formation. Tetrahedral DNA nanostructures (TDN), promising nucleic acid nanomaterials, have garnered attention for their potential in osteoporotic mandibular owing ability enhance cellular activity and promote osteogenic differentiation. Osteoblasts play a critical role regeneration; however, intracellular delivery of TDN into osteoblasts remains difficult. In this study, novel osteoblast-targeted CH6 aptamer-functionalized (TDN-CH6) aimed develop regeneration. This results demonstrated that TDN-CH6 exhibits superior osteoblast specificity efficient recruitment fracture sites. Furthermore, significantly enhances differentiation compared alone. Notably, Gelatin Methacryloyl (GelMA) hydrogels incorporating shows improved biological performance are favorable regeneration, suggesting platform represents strategy addressing complex defects.

Language: Английский

Citations

0