Siderophore-producing Bacillus amyloliquefaciens BM3 mitigate arsenic contamination and suppress Fusarium wilt in brinjal plants DOI

Smriti Pradhan,

Abhinandan Choudhury,

Sovan Dey

et al.

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(10)

Published: Sept. 22, 2023

Arsenic contamination in agricultural soils poses a serious health risk for humans. Bacteria that produce siderophores, primarily iron acquisition, can be relevant combating arsenic toxicity and simultaneously act as biocontrol agents against plant diseases. We evaluated the bioremediation potential of rhizosphere isolate Bacillus amyloliquefaciens BM3 studied interaction between purified siderophore bacillibactin arsenic.BM3 showed high resistance [MIC value 475 24 mM As(V) As(III), respectively] broad spectrum in-vitro antagonism several phytopathogenic fungi. was identified by biochemical characterization 16S rRNA gene sequencing. Scanning electron microscopy (SEM) analysis revealed increased cell size when grown presence sub-lethal concentrations. Bioremediation assays 74% 88.1% reduction As(III) concentrations, respectively. Genetic determinants (arsC aoxB) antifungal traits (bacAB chiA) were detected PCR. chelating ability bacillibactin, from culture filtrate through spectroscopic data analysis, observed CAS assay fluorescence spectrometry. In-vivo application talc-based formulation brinjal seedlings significant Fusarium wilt disease.Strain B. may useful considered large field trials an alternative to chemical fungicides inhibiting soil borne pathogens.

Language: Английский

Antimicrobial Metabolites of Bacillus velezensis FZB42 Reshape Rice Rhizosphere Microbial Community Composition and Induce Host Resistance Against Rhizoctonia solani DOI Creative Commons
Qurban Ali,

Abdur Rashid Khan,

Yujie Wang

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: 41, P. 100440 - 100440

Published: Jan. 15, 2025

Language: Английский

Citations

2
Plant-Microbe Interaction: Mining the Impact of Native Bacillus amyloliquefaciens WS-10 on Tobacco Bacterial Wilt Disease and Rhizosphere Microbial Communities DOI Creative Commons
Waqar Ahmed,

Zhenlin Dai,

Jinhao Zhang

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(4)

Published: Aug. 1, 2022

The plant rhizosphere acts as the first line of defense against invasion pathogens. perturbation in microbiome is directly related to health and disease development.

Language: Английский

Citations

68
Biodegradable microplastics reduce the effectiveness of biofertilizers by altering rhizospheric microecological functions DOI
Xinyang Li,

Jialing Wu,

Xueyu Cheng

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 352, P. 120071 - 120071

Published: Jan. 20, 2024

Language: Английский

Citations

9
Molecular Characterization Reveals Biodiversity and Biopotential of Rhizobacterial Isolates of Bacillus Spp DOI Creative Commons

Alka Sagar,

Shalini Rai,

Sonia Sharma

et al.

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: June 18, 2024

Abstract Bacillus species appearas the most attractive plant growth-promoting rhizobacteria (PGPR) and alternative to synthetic chemical pesticides. The present study examined antagonistic potential of spore forming- Bacilli isolated from organic farm soil samples Allahabad, India. Eighty-seven strains were characterized based on their morphological, growth promoting traits molecular characteristics. diversity analysis used 16S-rDNA, BOX-element, enterobacterial repetitive intergenic consensus. Two strains, PR30 PR32, later identified as sp., exhibited potent in vitro activity against Ralstonia solanaceorum . These isolates produced copious amounts multiple PGP traits, such indole-3-acetic acid (40.0 54.5 μg/mL), phosphate solubilization index (PSI) (4.4 5.3), ammonia, siderophore (3 4 cm), 1-aminocyclopropane-1-carboxylate deaminase (8.1and 9.2 μM/mg//h) hydrogen cyanide. subjected antibiotic sensitivity test. two higher best ability selected for response studies tomatoe, broccoli, chickpea. In pot study, subtilis (PR30 PR31) showed significant improvement seed germination (27–34%), root length (20–50%), shoot (20–40%), vigor (50–75%), carotenoid content (0.543–1.733), lycopene (2.333–2.646 mg/100 g) tomato, demonstrated production by effective bioinoculants promotion biocontrol phytopathogens.

Language: Английский

Citations

9
Effective Applications of Bacillus subtilis and B. amyloliquefaciens as Biocontrol Agents of Damping-Off Disease and Biostimulation of Tomato Plants DOI Creative Commons
Dina Fathi Ismail Ali, Sahar El-Nahrawy,

Hassan A. H. EL-Zawawy

et al.

Stresses, Journal Year: 2025, Volume and Issue: 5(1), P. 9 - 9

Published: Jan. 27, 2025

Using Bacillus species as bioagents for environmentally sustainable and economically viable plant disease management is a strategy. Thus, it important to promote their use in agriculture. In this study, two were isolated from the rhizosphere of tomato plants, while three fungal samples plants that infected with damping-off disease. The strains tested vitro antagonistic activity against using dual culture technique. greenhouse experiment, effectiveness applying bacteria soilborne on induced (cv. Super Strain B) physiological attributes, antioxidant enzymes, mineral content, yield under conditions during 2022 2023 seasons determined. isolates identified Fusarium oxysporum KT224063, Pythium debaryanum OP823136, Rhizoctonia solani OP823124, B. subtilis OP823140 amyloliquefaciens OP823147 basis rRNA gene sequences. test revealed outperformed resistance R. F. oxysporum, which recorded 28.33 33.00 mm, respectivley. contrast, caused highest effect P. fungus. Additionally, treated each these significantly suppressed disease, displayed improved growth parameters, had an increased content photosynthetic pigments, antioxidants total phenols, macronutrient growing seasons. conclusion, effective applications potential mitigate by debaryanum, simultaneously promoting dynamics.

Language: Английский

Citations

1
Biocontrol efficacy of Bacillus licheniformis and Bacillus amyloliquefaciens against rice pathogens DOI Creative Commons
Mohsin Tariq, Mehvish Zahoor, Tahira Yasmeen

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18920 - e18920

Published: Jan. 29, 2025

Biocontrol is a cost-effective and eco-friendly approach to control plant pathogens using natural enemies. Antagonistic microorganisms or their derivatives specifically target the while minimizing harm non-target organisms. Bacterial blight brown spot are major rice diseases caused by Xanthomonas oryzae pv. ( Xoo ) Bipolaris Bo ), respectively. This study was conducted assess growth-promoting potential biocontrol activity of root-associated bacteria against pathogens, . A total 98 were isolated from roots characterized for properties including phosphate solubilization, indole-3-acetic acid production, nitrogen fixation biofilm formation. Based on these properties, 36 selected tested via co-culturing antagonism assay. LE7 exhibited maximum inhibition 79%, FR8, PE2, LE7, LR22 LR28 also significantly reduced growth Likewise, FR2, LR22, LR35 inhibited , in which 81%. Under controlled-conditions, disease incidence respectively, improved rice. Full-length 16S rRNA gene sequencing most bacterial isolates, revealed identity with Bacillus amyloliquefaciens licheniformis Application spp. as agent represents enormous farming. The promising isolates could be used bioinoculants management production sustainable manner.

Language: Английский

Citations

1
Controlling mildew of tobacco leaf by Bacillus amyloliquefaciens ZH-2 and its effect on storage quality of tobacco leaf DOI Creative Commons
Hang Zhou, Yongfeng Yang, Jia Tao

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 12, 2025

Language: Английский

Citations

1
Unraveling the Mechanism of the Endophytic Bacterial Strain Pseudomonas oryzihabitans GDW1 in Enhancing Tomato Plant Growth Through Modulation of the Host Transcriptome and Bacteriome DOI Open Access
Waqar Ahmed, Yan Wang,

Wenxia Ji

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1922 - 1922

Published: Feb. 23, 2025

Endophytic Pseudomonas species from agricultural crops have been extensively studied for their plant-growth-promoting (PGP) potential, but little is known about PGP potential when isolated perennial trees. This study investigated the of an endophyte, oryzihabitans GDW1, a healthy pine tree by taking tomato as host plant. We employed multiomics approaches (transcriptome and bacteriome analyses) to elucidate underlying mechanisms GDW1. The results greenhouse experiments revealed that application GDW1 significantly improved plant growth, increasing shoot length, root fresh weight, biomass accumulation up 44%, 38%, 54%, 59%, respectively, compared with control. Transcriptomic analysis 1158 differentially expressed genes enriched in hormone signaling (auxin, gibberellin, cytokinin) stress response (plant-pathogen interaction, MAPK pathway-plant, phenylpropanoid biosynthesis) pathways. Protein-protein interaction network nine hub (MAPK10, ARF19-1, SlCKX1, GA2ox2, PAL5, SlWRKY37, GH3.6, XTH3, NML1) related tolerance, control, defense. Analysis through 16S rRNA gene amplicon sequencing inoculation dramatically altered bacterial community structure, enhancing diversity abundance beneficial taxa (Proteobacteria Bacteroidota). Co-occurrence showed complex treated plants, suggesting increasingly intricate microbial relationships nutrient absorption. Additionally, FAPROTAX PICRUSt2 functional prediction analyses suggested role nitrogen cycling, organic matter degradation, growth promotion, resistance. In conclusion, this provides novel insights into symbiotic relationship between P. highlighting its biofertilizer sustainable agriculture means reducing reliance on agrochemicals.

Language: Английский

Citations

1
Evaluation of Antifungal Activity of Endophytic Bacillus spp. and Identification of Secondary Metabolites Produced Against the Phytopathogenic Fungi DOI
Janki K. Patel,

Y Mistry,

Riya Soni

et al.

Current Microbiology, Journal Year: 2024, Volume and Issue: 81(5)

Published: April 5, 2024

Language: Английский

Citations

7
Potential biocontrol efficiency of Trichoderma species against oomycete pathogens DOI Creative Commons
Yinglong Liu,

Pengbo He,

Pengfei He

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 6, 2022

Plant health is of utmost importance for optimal agricultural production and sustainability. Unfortunately, biotic abiotic factors put a major constraint on crop safety productivity. diseases caused by oomycetes inflict serious damage to various crops. Moreover, the injudicious use chemical pesticides poses threats related pesticide resistance development in pathogens environmental pollution. Biocontrol offers an effective solution disease control; however, research biocontrol oomycete-related scarce. Thus, this study undertakes screening resources management plant diseases. In regard, 86 isolates Trichoderma spp. were assessed against Phytophthora nicotianae, P. capsici, Pythium vexans, ultimum, dissotocum through dual culture assay. Furthermore, antagonistic effect selected was studied tobacco black shank damping-off cucumber seedlings greenhouse. The relative control three strains AR-4, Tv-1, ST4-1 more than 60%, which not significantly different from 6.88 gl-1 fluopicolide-propamocarb. Whereas, AR-4 80.33% 82.67%, respectively, higher Tv-1 (35.49%) fluopicolide-propamocarb (47.82%). According morphological molecular characterization, fungal identified as koningiopsis, T. asperellum, gamsii, respectively. conclusion, exhibited strong oomycete can be integrated into strategies.

Language: Английский

Citations

25