Journal of Cell Science,
Journal Year:
2022,
Volume and Issue:
135(7)
Published: April 1, 2022
Neurons
extend
axons
to
form
the
complex
circuitry
of
mature
brain.
This
depends
on
coordinated
response
and
continuous
remodelling
microtubule
F-actin
networks
in
axonal
growth
cone.
Growth
cone
architecture
remains
poorly
understood
at
nanoscales.
We
therefore
investigated
mouse
hippocampal
neuron
cones
using
cryo-electron
tomography
directly
visualise
their
three-dimensional
subcellular
with
molecular
detail.
Our
data
showed
that
hexagonal
arrays
actin
bundles
filopodia
penetrate
terminate
deep
within
interior.
observed
modulation
these
other
by
alteration
individual
helical
structures.
Microtubules
blunt,
slightly
flared
or
gently
curved
ends
predominated
cone,
frequently
contained
lumenal
particles
exhibited
lattice
defects.
Investigation
effect
absence
doublecortin,
a
neurodevelopmental
cytoskeleton
regulator,
no
major
anomalies
overall
organisation
subpopulations.
However,
our
suggested
microtubules
sustained
more
structural
defects,
highlighting
importance
integrity
during
migration.
Annual Review of Cell and Developmental Biology,
Journal Year:
2021,
Volume and Issue:
37(1), P. 311 - 340
Published: Aug. 10, 2021
Calcium
(Ca
2+
)
is
a
unique
mineral
that
serves
as
both
nutrient
and
signal
in
all
eukaryotes.
To
maintain
Ca
homeostasis
for
nutrition
signaling
purposes,
the
tool
kit
transport
has
expanded
across
kingdoms
of
eukaryotes
to
encode
specific
signals
referred
signatures.
In
parallel,
large
array
-binding
proteins
evolved
sensors
decode
By
comparing
these
coding
decoding
mechanisms
fungi,
animals,
plants,
unified
divergent
themes
have
emerged,
underlying
complexity
will
challenge
researchers
years
come.
Considering
scale
breadth
subject,
instead
literature
survey,
this
review
we
focus
on
conceptual
framework
aims
introduce
readers
principles
signaling.
We
finish
with
several
examples
-signaling
pathways,
including
polarized
cell
growth,
immunity
symbiosis,
systemic
signaling,
piece
together
plants
versus
animals.
Antioxidants,
Journal Year:
2021,
Volume and Issue:
10(1), P. 98 - 98
Published: Jan. 12, 2021
Mitochondria
are
structurally
and
functionally
distinct
organelles
that
produce
adenosine
triphosphate
(ATP)
through
oxidative
phosphorylation
(OXPHOS),
to
provide
energy
spermatozoa.
They
can
also
reactive
oxidation
species
(ROS).
While
a
moderate
concentration
of
ROS
is
critical
for
tyrosine
in
cholesterol
efflux,
sperm–egg
interaction,
fertilization,
excessive
generation
associated
with
male
infertility.
Moreover,
mitochondria
participate
diverse
processes
ranging
from
spermatogenesis
fertilization
regulate
fertility.
This
review
aimed
summarize
the
roles
fertility
depending
on
sperm
developmental
stage
(from
reproductive
tract
female
tract).
involved
testosterone
production,
regulation
proton
secretion
into
lumen
maintain
an
acidic
condition
epididymis,
DNA
condensation
during
epididymal
maturation.
We
established
new
signaling
pathway
using
previous
proteomic
data
fertility,
understand
overall
role
The
revealed
infertility
loss
mitochondrial
proteins
spermatozoa,
which
induces
low
motility,
reduces
OXPHOS
activity,
results
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: May 30, 2023
Abstract
Three-dimensional
hydrogel-based
organ-like
cultures
can
be
applied
to
study
development,
regeneration,
and
disease
in
vitro.
However,
the
control
of
engineered
hydrogel
composition,
mechanical
properties
geometrical
constraints
tends
restricted
initial
time
fabrication.
Modulation
characteristics
over
according
culture
evolution
is
often
not
possible.
Here,
we
overcome
these
limitations
by
developing
a
hydrogel-in-hydrogel
live
bioprinting
approach
that
enables
dynamic
fabrication
instructive
elements
within
pre-existing
cultures.
This
achieved
crosslinking
photosensitive
hydrogels
via
two-photon
absorption
at
any
during
culture.
We
show
guide
neural
axon
directionality
growing
organotypic
spinal
cords,
geometry
differential
cell
migration
cancer
organoids.
Finally,
promote
polarity
liver
organoids,
small
intestinal
organoid
morphogenesis
lung
tip
bifurcation
composition
shape.
Bone Research,
Journal Year:
2023,
Volume and Issue:
11(1)
Published: Jan. 5, 2023
Abstract
Skeletal
tissue
is
highly
innervated.
Although
different
types
of
nerves
have
been
recently
identified
in
the
bone,
crosstalk
between
bone
and
remains
unclear.
In
this
review,
we
outline
role
peripheral
nervous
system
(PNS)
regeneration
following
injury.
We
first
introduce
conserved
species
ranging
from
amphibians
to
mammals.
then
present
distribution
PNS
skeletal
under
physiological
conditions,
fractures,
or
regeneration.
Furthermore,
summarize
ways
which
communicates
with
bone-lineage
cells,
vasculature,
immune
cells
microenvironment.
Based
on
comprehensive
timely
conclude
that
regulates
through
neuropeptides
neurotransmitters
nerves.
An
in-depth
understanding
roles
will
inform
development
new
strategies
based
bone-nerve
promoting
repair
