Engineering organoids

Nature Reviews Materials, Journal Year: 2021, Volume and Issue: 6(5), P. 402 - 420

Published: Feb. 19, 2021

Language: Английский

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Sliced Human Cortical Organoids for Modeling Distinct Cortical Layer Formation

Cell stem cell, Journal Year: 2020, Volume and Issue: 26(5), P. 766 - 781.e9

Published: March 9, 2020

Language: Английский

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Immune cell regulation of glia during CNS injury and disease

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 21(3), P. 139 - 152

Published: Feb. 10, 2020

Language: Английский

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Towards organoid culture without Matrigel

Communications Biology, Journal Year: 2021, Volume and Issue: 4(1)

Published: Dec. 10, 2021

Organoids-cellular aggregates derived from stem or progenitor cells that recapitulate organ function in miniature-are of growing interest developmental biology and medicine. Organoids have been developed for organs tissues such as the liver, gut, brain, pancreas; they are used surrogates to study a wide range questions basic biology, genetic disorders, therapies. However, many organoids reported date cultured Matrigel, which is prepared secretion Engelbreth-Holm-Swarm mouse sarcoma cells; Matrigel complex poorly defined. This complexity makes it difficult elucidate Matrigel-specific factors governing organoid development. In this review, we discuss promising Matrigel-free methods generation maintenance use decellularized extracellular matrix (ECM), synthetic hydrogels, gel-forming recombinant proteins.

Language: Английский

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Microglia/Brain Macrophages as Central Drivers of Brain Tumor Pathobiology

Neuron, Journal Year: 2019, Volume and Issue: 104(3), P. 442 - 449

Published: Nov. 1, 2019

Language: Английский

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Identification of neural oscillations and epileptiform changes in human brain organoids

Nature Neuroscience, Journal Year: 2021, Volume and Issue: 24(10), P. 1488 - 1500

Published: Aug. 23, 2021

Language: Английский

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Autism spectrum disorder at the crossroad between genes and environment: contributions, convergences, and interactions in ASD developmental pathophysiology

Molecular Autism, Journal Year: 2020, Volume and Issue: 11(1)

Published: Sept. 10, 2020

The complex pathophysiology of autism spectrum disorder encompasses interactions between genetic and environmental factors. On the one hand, hundreds genes, converging at functional level on selective biological domains such as epigenetic regulation synaptic function, have been identified to be either causative or risk factors autism. other exposure chemicals that are widespread in environment, endocrine disruptors, has associated with adverse effects human health, including neurodevelopmental disorders. Interestingly, experimental results suggest an overlap regulatory pathways perturbed by mutations factors, depicting convergences interplays susceptibility toxic insults. pervasive nature chemical poses pivotal challenges for neurotoxicological studies, agencies, policy makers. This highlights emerging need developing new integrative models, biomonitoring, epidemiology, experimental, computational tools, able capture real-life scenarios encompassing interaction chronic mixture substances individuals' backgrounds. In this review, we address intertwined roles lesions Specifically, outline transformative potential stem cell coupled omics analytical approaches increasingly single resolution, tools experimentally dissect pathogenic mechanisms underlying disorders, well improve developmental neurotoxicology assessment.

Language: Английский

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A nomenclature consensus for nervous system organoids and assembloids

Nature, Journal Year: 2022, Volume and Issue: 609(7929), P. 907 - 910

Published: Sept. 28, 2022

Language: Английский

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Human cerebral organoids — a new tool for clinical neurology research

Nature Reviews Neurology, Journal Year: 2022, Volume and Issue: 18(11), P. 661 - 680

Published: Oct. 17, 2022

Language: Английский

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Organoid intelligence (OI): the new frontier in biocomputing and intelligence-in-a-dish

Frontiers in Science, Journal Year: 2023, Volume and Issue: 1

Published: Feb. 28, 2023

Recent advances in human stem cell-derived brain organoids promise to replicate critical molecular and cellular aspects of learning memory possibly cognition vitro . Coining the term “organoid intelligence” (OI) encompass these developments, we present a collaborative program implement vision multidisciplinary field OI. This aims establish OI as form genuine biological computing that harnesses using scientific bioengineering an ethically responsible manner. Standardized, 3D, myelinated can now be produced with high cell density enriched levels glial cells gene expression for learning. Integrated microfluidic perfusion systems support scalable durable culturing, spatiotemporal chemical signaling. Novel 3D microelectrode arrays permit high-resolution electrophysiological signaling recording explore capacity recapitulate mechanisms formation and, ultimately, their computational potential. Technologies could enable novel biocomputing models via stimulus-response training organoid-computer interfaces are development. We envisage complex, networked whereby connected real-world sensors output devices, ultimately each other sensory organ (e.g. retinal organoids), trained biofeedback, big-data warehousing, machine methods. In parallel, emphasize embedded ethics approach analyze ethical raised by research iterative, manner involving all relevant stakeholders. The many possible applications this urge strategic development discipline. anticipate OI-based allow faster decision-making, continuous during tasks, greater energy data efficiency. Furthermore, “intelligence-in-a-dish” help elucidate pathophysiology devastating developmental degenerative diseases (such dementia), potentially aiding identification therapeutic approaches address major global unmet needs.

Language: Английский

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