The expression of exosomal and cellular miRNAs in predicting oxaliplatin resistance in colorectal cancer cells: an in silico and in vitro study DOI Creative Commons

Hoda Deli,

Zeynab Vakili-Ghartavol,

Yazdan Asgari

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 9, 2025

Colorectal cancer (CRC) is a common gastrointestinal cancer, and even though oxaliplatin chemotherapy effective, there high likelihood of relapse, indicating the presence oxaliplatin-resistant CRC. Therefore, it crucial to comprehend molecular mechanisms resistance develop effective strategies counter drug resistance. Numerous studies have demonstrated close association between microRNAs (miRNAs) in In this study, we aimed identify essential exosomal cellular miRNA related CRC cell line HCT-116. The expression profile cells with was analyzed. effectiveness diagnostics biomarker potency miRNAs were evaluated by receiver operating characteristic (ROC) analysis. Target identified, enrichment analysis assessed based on Gene Ontology (GO), Reactome, Human Disease (DO). vitro experiments, HCT-116 (HCT116-OXA) developed, exosomes isolated characterized from HCT116-OXA HCT116 cells. selected their exosomes, they compared using quantitative real-time PCR. This study revealed that combination miR-4326, miR-3615, miR-7974, miR-130b-3p, miR-454-3p exhibited highest area under curve (AUC), sensitivity, specificity, superior diagnostic predictive performance. displayed reduced early late apoptosis, bypass S phase arrest, prolonged doubling time, higher IC50 parental miR-454-3p, miR-4326 decreased as sensitive However, significantly miR-130b-3p observed low miR-3615 or could predict response therapy. indicates potential these specific serve markers for

Language: Английский

Cancer-associated fibroblasts suppress ferroptosis and induce gemcitabine resistance in pancreatic cancer cells by secreting exosome-derived ACSL4-targeting miRNAs DOI Creative Commons
Ran Qi,

Yixuan Bai,

Kun Li

et al.

Drug Resistance Updates, Journal Year: 2023, Volume and Issue: 68, P. 100960 - 100960

Published: March 28, 2023

Pancreatic cancer continues to be one of the world's most lethal cancers. Chemotherapy resistance in patients with advanced pancreatic often accompany dismal prognosis, highlighting need investigate mechanisms drug and develop therapies overcome chemoresistance.This research was filed Chinese Clinical Trial Registry (ChiCTR2200061320). In order isolate primary normal fibroblasts (NFs) cancer-associated (CAFs) samples ductal adenocarcinoma (PDAC) paracancerous tissue from individuals diagnosed PDAC were obtained. The exosomes obtained using ultracentrifugation, their characteristics determined by Western blotting, nanoparticle tracking analysis, transmission electron microscopy. CAF-derived miRNAs analyzed RT-qPCR high-throughput sequencing. Gemcitabine (GEM) employed promote ferroptosis, ferroptosis levels monitoring lipid reactive oxygen species (ROS), cell survival, intracellular Fe2+ concentrations. To assess vivo tumor response GEM therapy, a xenograft mouse model utilized.Exosomes derived CAFs did not exhibit innate resistance. promoted chemoresistance cells following treatment secreting exosomes, maintaining signaling communication cells. Mechanistically, miR-3173-5p CAF sponged ACSL4 inhibited after uptake cells.This work demonstrates novel mode acquired identifies miR-3173-5p/ACSL4 pathway as promising target for GEM-resistant cancer.

Language: Английский

Citations

148
The Functional Meaning of 5′UTR in Protein-Coding Genes DOI Open Access
Natalia Ryczek, Aneta Łyś, Izabela Makałowska

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2976 - 2976

Published: Feb. 3, 2023

As it is well known, messenger RNA has many regulatory regions along its sequence length. One of them the 5' untranslated region (5'UTR), which itself contains elements such as upstream ORFs (uORFs), internal ribosome entry sites (IRESs), microRNA binding sites, and structural components involved in regulation mRNA stability, pre-mRNA splicing, translation initiation. Activation alternative, more transcription start site leads to an extension 5'UTR. consequences 5'UTRs may be head-to-head gene overlap. This review describes 5'UTR protein-coding transcripts functional significance genes overlap with implications for transcription, translation, disease.

Language: Английский

Citations

62
AI-Enhanced Visual-Spectral Synergy for Fast and Ultrasensitive Biodetection of Breast Cancer-Related miRNAs DOI
Wei Wang, Lei Liu, Jianxiong Zhu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(8), P. 6266 - 6275

Published: Jan. 22, 2024

In biomedical testing, artificial intelligence (AI)-enhanced analysis has gradually been applied to the diagnosis of certain diseases. This research employs AI algorithms refine precision integrative detection, encompassing both visual results and fluorescence spectra from lateral flow assays (LFAs), which signal presence cancer-linked miRNAs. Specifically, color shift gold nanoparticles (GNPs) is paired with red nitrogen vacancy centers (NV-centers) in fluorescent nanodiamonds (FNDs) integrated into LFA strips. While GNPs amplify FNDs, turn, FNDs enhance intensity GNPs. reciprocal intensification can be synergistically augmented algorithms, thereby improving detection sensitivity for early diagnosis. Supported by platform based on this strategy, fastest a limit (LOD) at fM level R2 value ∼0.9916 miRNA obtained within 5 min. Meanwhile, labeling capture probes miRNA-21 miRNA-96 (both are indicators breast cancer) separate T-lines, simultaneous them achieved. The methods employed study may potentially future cancer.

Language: Английский

Citations

23
Utilizing non-coding RNA-mediated regulation of ATP binding cassette (ABC) transporters to overcome multidrug resistance to cancer chemotherapy DOI
Kenneth K.W. To,

Zoufang Huang,

Hang Zhang

et al.

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 73, P. 101058 - 101058

Published: Jan. 19, 2024

Language: Английский

Citations

20
Osteomyelitis and non-coding RNAS: A new dimension in disease understanding DOI
Md Sadique Hussain, Nusrat K. Shaikh, Mohit Agrawal

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 255, P. 155186 - 155186

Published: Feb. 2, 2024

Language: Английский

Citations

18
Exosomal microRNAs in regulation of tumor cells resistance to apoptosis DOI Creative Commons
Mohammad Salehi, Mohammad Javad Kamali,

Daniyal Arab

et al.

Biochemistry and Biophysics Reports, Journal Year: 2024, Volume and Issue: 37, P. 101644 - 101644

Published: Jan. 17, 2024

Exosomes are a type of extracellular vesicle that contains bioactive molecules can be secreted by most cells. Nevertheless, the content these cells differs depending on cell from which they originate. The exosome plays crucial role in modulating intercellular communication conveying molecular messages to neighboring or distant Cancer-derived exosomes transfer several types into tumor microenvironment, including high levels microRNA (miRNA). These miRNAs significantly affect proliferation, angiogenesis, apoptosis resistance, metastasis, and immune evasion. Increasing evidence indicates exosomal (exomiRs) regulating cancer resistance apoptosis. In cells, exomiRs orchestrate channels between them their surrounding gene expression controlling signaling pathways. This review presents an outline present-day knowledge mechanisms target drive Also, our study looks at regulatory mediating microenvironmental specifically stromal evade therapy-induced

Language: Английский

Citations

17
The crosstalk between exosomal miRNA and ferroptosis: A narrative review DOI
Zahra Nashtahosseini, Masoumeh Nejatollahi, Ahmad Fazilat

et al.

Biology of the Cell, Journal Year: 2025, Volume and Issue: 117(1)

Published: Jan. 1, 2025

Ferroptosis is a type of cell death that multiple mechanisms and pathways contribute to the positive negative regulation it. For example, increased levels reactive oxygen species (ROS) induce ferroptosis. ferroptosis unlike apoptosis, it not dependent on caspases, but iron. Exosomes are membrane-bound vesicles with size about 30 150 nm, contain various cellular components, including DNA, RNA, microRNAs (miRNAs), lipids, proteins, which genetically similar their cells origin. found in all bodily fluids, blood, saliva, urine. Cells often release exosomes after fusion membrane. They play an important role immune cell-cell communication. miRNAs, noncoding RNAs length 18 24 nucleotides, involved regulating gene expression transcription. Emerging data suggests exosomal miRNAs implicated pathophysiological cells, metastasis, drug resistance, death. In addition, functional studies have indicated can key modulation by Therefore, this review, given importance ferroptosis, we decided elucidate relationship between diseases.

Language: Английский

Citations

3
miRNAs in neurodegenerative diseases: from target screening to precision therapy DOI
Dongyi Liao, Yujie Zhang, Shuangyang Li

et al.

Neurological Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

3
A systematic review of the research progress of non-coding RNA in neuroinflammation and immune regulation in cerebral infarction/ischemia-reperfusion injury DOI Creative Commons
Kailin Yang,

Liuting Zeng,

Anqi Ge

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 7, 2022

Cerebral infarction/ischemia-reperfusion injury is currently the disease with highest mortality and disability rate of cardiovascular disease. Current studies have shown that nerve cells die ischemia several hours after ischemic stroke, which activates innate immune response in brain, promotes production neurotoxic substances such as inflammatory cytokines, chemokines, reactive oxygen species − nitrogen oxide, mediates destruction blood-brain barrier occurrence a series cascade reactions. Meanwhile, expression adhesion molecules cerebral vascular endothelial increased, polymorphonuclear neutrophils, lymphocytes mononuclear macrophages passed through entered brain tissue. These recognize antigens exposed by central nervous system activate adaptive responses, further mediate secondary neuronal damage, aggravating neurological deficits. In order to reduce above-mentioned body induces peripheral immunosuppressive responses negative feedback, increases incidence post-stroke infection. This process accompanied changes status tissue local systemic systems. A growing number implicate noncoding RNAs (ncRNAs) novel epigenetic regulatory elements dysfunction various cell subsets neurovascular unit injury. particular, recent revealed advances ncRNA biology greatly expand understanding regulation inflammation Identification aberrant patterns associated biological effects ncRNAs patients their potential biomarkers therapeutic targets for Therefore, this review systematically presents on involvement neuroimmune cascades, elucidates functions mechanisms infarction/ischemia-reperfusion-related ncRNAs, providing new opportunities discovery targeted therapy. Furthermore, introduces clustered regularly interspaced short palindromic repeats (CRISPR)-Display possible transformative tool studying lncRNAs. future, expected be used target diagnosing injury, judging its prognosis treatment, thereby significantly improving patients.

Language: Английский

Citations

61
MicroRNA-206 in human cancer: Mechanistic and clinical perspectives DOI

Leila Bahari Khasraghi,

Morteza Nouri,

Masoud Vazirzadeh

et al.

Cellular Signalling, Journal Year: 2022, Volume and Issue: 101, P. 110525 - 110525

Published: Nov. 16, 2022

Language: Английский

Citations

40