Analyzing Amylin Aggregation Inhibition Through Quantum Dot Fluorescence Imaging DOI Open Access
Xiaoyu Yin, Ziwei Liu,

Gegentuya Huanood

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11132 - 11132

Published: Oct. 17, 2024

Protein aggregation is associated with various diseases caused by protein misfolding. Among them, amylin deposition a prominent feature of type 2 diabetes. At present, the mechanism remains unclear, and this has hindered treatment In study, we analyzed process using quantum dot (QD) imaging method. QD fluorescence revealed that in presence 100 μM amylin, aggregates appeared after 12 h incubation, while large number formed 24 standard deviation (SD) value 5.435. contrast, 50 did not induce formation although were observed an SD 2.883. Confocal laser microscopy observations these deposited three dimensions. Transmission electron existed as misfolded fibrils vitro QDs uniformly bound to fibrils. addition, microliter-scale high-throughput screening (MSHTS) system, found rosmarinic acid, polyphenol, inhibited at half-maximal effective concentration 852.8 μM. These results demonstrate MSHTS system powerful tool for evaluating inhibitory activity aggregation. Our findings will contribute understanding pathogenesis amylin-related discovery compounds may be useful prevention diseases.

Language: Английский

Type 2 Diabetes Mellitus: A Comprehensive Review of Pathophysiology, Comorbidities, and Emerging Therapies DOI
Aditi Singh,

Sucharita Shadangi,

Pulkit Kr. Gupta

et al.

Comprehensive physiology, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 1, 2025

ABSTRACT Humans are perhaps evolutionarily engineered to get deeply addicted sugar, as it not only provides energy but also helps in storing fats, which survival during starvation. Additionally, sugars (glucose and fructose) stimulate the feel‐good factor, they trigger secretion of serotonin dopamine brain, associated with reward sensation, uplifting mood general. However, when consumed excess, contributes imbalance, weight gain, obesity, leading onset a complex metabolic disorder, generally referred diabetes. Type 2 diabetes mellitus (T2DM) is one most prevalent forms diabetes, nearly affecting all age groups. T2DM clinically diagnosed cardinal sign chronic hyperglycemia (excessive sugar blood). Chronic hyperglycemia, coupled dysfunctions pancreatic β‐cells, insulin resistance, immune inflammation, further exacerbate pathology T2DM. Uncontrolled T2DM, major public health concern, significantly toward progression several micro‐ macrovascular diseases, such diabetic retinopathy, nephropathy, neuropathy, atherosclerosis, cardiovascular including cancer. The current review discusses epidemiology, causative factors, pathophysiology, comorbidities, existing emerging therapies related It future roadmap for alternative drug discovery management

Language: Английский

Citations

1
Exploring the Significance of Gut Microbiota in Diabetes Pathogenesis and Management—A Narrative Review DOI Open Access
Ewelina Młynarska, Jakub Wasiak, Agata Gajewska

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(12), P. 1938 - 1938

Published: June 19, 2024

Type 2 diabetes is a disease with significant health consequences for the individual. Currently, new mechanisms and therapeutic approaches that may affect this are being sought. One of them association type microbiota. Through enteric nervous system gut–microbiota axis, microbiota affects functioning body. It has been proven to have real impact on influencing glucose lipid metabolism insulin sensitivity. With dysbiosis, there increased bacterial translocation through disrupted intestinal barrier inflammation in In diabetes, microbiota’s composition altered with, example, more abundant class Betaproteobacteria. The these disorders linked involving short-chain fatty acids, branched-chain amino lipopolysaccharide, among others. Interventions focusing gut gaining traction as promising approach management. Studies currently conducted effects supply probiotics prebiotics, well fecal transplantation, course diabetes. Further research will allow us fully develop our knowledge subject possibly best treat prevent

Language: Английский

Citations

6
Next-Generation Probiotics and Chronic Diseases: A Review of Current Research and Future Directions DOI Creative Commons
Ashutosh Tiwari,

Dyah Ika Krisnawati,

Erna Susilowati

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(50), P. 27679 - 27700

Published: Nov. 26, 2024

The burgeoning field of microbiome research has profoundly reshaped our comprehension human health, particularly highlighting the potential probiotics and fecal microbiota transplantation (FMT) as therapeutic interventions. While benefits traditional are well-recognized, efficacy mechanisms remain ambiguous, FMT's long-term effects still being investigated. Recent advancements in high-throughput sequencing have identified gut microbes with significant health benefits, paving way for next-generation (NGPs). These NGPs, engineered through synthetic biology bioinformatics, designed to address specific disease states enhanced stability viability. This review synthesizes current on NGP stability, challenges delivery, their applications preventing treating chronic diseases such diabetes, obesity, cardiovascular diseases. We explore physiological characteristics, safety profiles, action various strains while also addressing opportunities presented by integration into clinical practice. NGPs revolutionize microbiome-based therapies improve outcomes is immense, underscoring need further optimize ensure safety.

Language: Английский

Citations

4
Gut Microbiota and Their Metabolites: The Hidden Driver of Diabetic Nephropathy? Unveiling Gut Microbe's Role in DN DOI Creative Commons

J. Liu,

Min Guo, Xiaobin Yuan

et al.

Journal of Diabetes, Journal Year: 2025, Volume and Issue: 17(4)

Published: April 1, 2025

ABSTRACT Background Diabetic nephropathy (DN) is a severe microvascular complication of diabetes with complex pathogenesis. Methods Recent studies were reviewed to explore the role gut microbiota and its metabolites in DN development. Results Dysbiosis bacteria contributes pathological changes such as glomerular sclerosis renal tubule injury. Microbial are involved through immune inflammatory pathways. Conclusions Understanding relationship between microbiota, metabolites, may offer potential implications for diagnosis, prevention, treatment. Translating this knowledge into clinical practice presents challenges opportunities.

Language: Английский

Citations

0
Ultrasonic degradation of mulberry twigs polysaccharides: Effect on in vitro hypoglycemic activity and prebiotic potential DOI

Yanan Jia,

Qiaoyu Li, Fenglin Jiang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143356 - 143356

Published: April 1, 2025

Language: Английский

Citations

0
Modulating gut microbiota in the management of type 2 diabetes mellitus: a narrative review DOI Open Access

Meirajuddin Tousif,

Maria Akhtar

International Journal of Research in Medical Sciences, Journal Year: 2024, Volume and Issue: 12(6), P. 2179 - 2185

Published: May 31, 2024

Type 2 diabetes mellitus (T2DM) represents a significant global health burden, necessitating innovative therapeutic approaches. Recent research has increasingly recognized the role of gut microbiota modulation in T2DM management, offering promising avenues for intervention. This systematic review synthesizes current literature investigating impact modulating on management. A comprehensive search databases yielded studies examining various strategies, including probiotics, prebiotics, dietary interventions, and facal transplantation. Analysis these interventions revealed their potential to improve glycemic control, insulin sensitivity, inflammation markers individuals with T2DM. Mechanistic insights elucidate how influences metabolic pathways, immune function, barrier integrity, thereby contributing pathophysiology. Furthermore, highlight interplay between composition host factors such as diet, lifestyle, genetics, underscoring complexity this relationship. Modulating presents approach benefits control health. However, further is warranted optimize intervention mechanistic explore long-term effects. The aim was underscores importance considering complementary multifaceted management

Language: Английский

Citations

0
Analyzing Amylin Aggregation Inhibition Through Quantum Dot Fluorescence Imaging DOI Open Access
Xiaoyu Yin, Ziwei Liu,

Gegentuya Huanood

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11132 - 11132

Published: Oct. 17, 2024

Protein aggregation is associated with various diseases caused by protein misfolding. Among them, amylin deposition a prominent feature of type 2 diabetes. At present, the mechanism remains unclear, and this has hindered treatment In study, we analyzed process using quantum dot (QD) imaging method. QD fluorescence revealed that in presence 100 μM amylin, aggregates appeared after 12 h incubation, while large number formed 24 standard deviation (SD) value 5.435. contrast, 50 did not induce formation although were observed an SD 2.883. Confocal laser microscopy observations these deposited three dimensions. Transmission electron existed as misfolded fibrils vitro QDs uniformly bound to fibrils. addition, microliter-scale high-throughput screening (MSHTS) system, found rosmarinic acid, polyphenol, inhibited at half-maximal effective concentration 852.8 μM. These results demonstrate MSHTS system powerful tool for evaluating inhibitory activity aggregation. Our findings will contribute understanding pathogenesis amylin-related discovery compounds may be useful prevention diseases.

Language: Английский

Citations

0