Regulation of sleep plasticity by a thermo-sensitive circuit in Drosophila DOI Creative Commons
Angélique Lamaze, Arzu Öztürk-Çolak,

Robin Fischer

et al.

Scientific Reports, Journal Year: 2017, Volume and Issue: 7(1)

Published: Jan. 13, 2017

Sleep is a highly conserved and essential behaviour in many species, including the fruit fly Drosophila melanogaster. In wild, sensory signalling encoding environmental information must be integrated with sleep drive to ensure that not initiated during detrimental conditions. However, molecular circuit mechanisms by which timing modulated environment are unclear. Here we introduce novel behavioural paradigm study this issue. We show male flies, onset of daytime siesta delayed ambient temperatures above 29 °C. term effect Prolonged Morning Wakefulness (PMW). through TrpA1 thermo-sensor required for PMW, specifically impacts onset, but night response elevated temperatures. identify two critical TrpA1-expressing circuits both contact DN1p clock neurons, output also PMW. Finally, circadian blue-light photoreceptor CRYPTOCHROME as regulator propose model nervous system integrates temperature, light, time dynamically control when initiated. Our results provide platform investigate how inputs co-ordinately regulate plasticity.

Language: Английский

Circadian Rhythms and Sleep in Drosophila melanogaster DOI Open Access

Christine Dubowy,

Amita Sehgal

Genetics, Journal Year: 2017, Volume and Issue: 205(4), P. 1373 - 1397

Published: March 30, 2017

The advantages of the model organism Drosophila melanogaster, including low genetic redundancy, functional simplicity, and ability to conduct large-scale screens, have been essential for understanding molecular nature circadian (∼24 hr) rhythms, continue be valuable in discovering novel regulators rhythms sleep. In this review, we discuss current these interrelated biological processes wider implications research. Clock genes period timeless were first discovered screens developed 1970s. Feedback on their own transcription forms core clock, accurately timed expression, localization, post-transcriptional modification, function is thought critical maintaining cycle. Regulators, several phosphatases kinases, act different steps feedback loop ensure strong rhythms. Approximately 150 neurons fly brain that contain components clock together translate intracellular cycling into rhythmic behavior. We how groups serve functions allowing clocks entrain environmental cues, driving behavioral outputs at times day, flexible responses conditions. neuropeptide PDF provides an important signal synchronize neurons, although details accomplishes are still being explored. Secreted signals from also influence other tissues. SLEEP is, part, regulated by which ensures appropriate timing sleep, but amount quality sleep determined mechanisms a homeostatic balance between wake. Flies useful identifying large set genes, molecules, neuroanatomic loci regulating amount. Conserved aspects regulation flies mammals include wake-promoting roles catecholamine neurotransmitters involvement hypothalamus-like regions, regions implicated less clear parallels. Sleep subject factors such as food availability, stress, social environment. beginning understand identified molecules interact with each other, environment, regulate researchers can take advantage increasing mechanistic behaviors, learning memory, courtship, aggression, loss impacts behaviors. thus remain tool both discovery deep

Language: Английский

Citations

372
Circadian neuron feedback controls the Drosophila sleep–activity profile DOI
Fang Guo, Junwei Yu,

Hyung Jae Jung

et al.

Nature, Journal Year: 2016, Volume and Issue: 536(7616), P. 292 - 297

Published: Aug. 1, 2016

Language: Английский

Citations

288
A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability DOI Creative Commons
Matthieu Flourakis,

Elżbieta Kula-Eversole,

Alan L. Hutchison

et al.

Cell, Journal Year: 2015, Volume and Issue: 162(4), P. 836 - 848

Published: Aug. 1, 2015

Language: Английский

Citations

209
Calcitonin Gene-Related Peptide Neurons Mediate Sleep-Specific Circadian Output in Drosophila DOI Creative Commons
Michael Kunst, Michael E. Hughes, Davide Raccuglia

et al.

Current Biology, Journal Year: 2014, Volume and Issue: 24(22), P. 2652 - 2664

Published: Oct. 30, 2014

Language: Английский

Citations

200
RNA-seq analysis of Drosophila clock and non-clock neurons reveals neuron-specific cycling and novel candidate neuropeptides DOI Creative Commons
Katharine C. Abruzzi, Zadina Abigail,

Weifei Luo

et al.

PLoS Genetics, Journal Year: 2017, Volume and Issue: 13(2), P. e1006613 - e1006613

Published: Feb. 9, 2017

Locomotor activity rhythms are controlled by a network of ~150 circadian neurons within the adult Drosophila brain. They subdivided based on their anatomical locations and properties. We profiled transcripts "around clock" from three key groups with different functions. also non-circadian outgroup, dopaminergic (TH) neurons. have cycling but fewer than clock as well low expression poor gene transcripts. This suggests that TH do not canonical is driven brain systemic cues. The surprisingly diverse in overall patterns, which include known putative novel neuropeptides. Even phase distributions distinct, indicating regulatory principles govern transcript oscillations. surprising cell-type diversity parallels functional heterogeneity

Language: Английский

Citations

144
A Series of Suppressive Signals within the Drosophila Circadian Neural Circuit Generates Sequential Daily Outputs DOI Creative Commons
Xitong Liang, Timothy E. Holy, Paul H. Taghert

et al.

Neuron, Journal Year: 2017, Volume and Issue: 94(6), P. 1173 - 1189.e4

Published: May 25, 2017

Language: Английский

Citations

139
Circadian rhythms in neuronal activity propagate through output circuits DOI
Matthieu Cavey,

Ben Collins,

Claire Bertet

et al.

Nature Neuroscience, Journal Year: 2016, Volume and Issue: 19(4), P. 587 - 595

Published: Feb. 29, 2016

Language: Английский

Citations

121
The molecular ticks of the Drosophila circadian clock DOI
Özgür Tataroğlu, Patrick Emery

Current Opinion in Insect Science, Journal Year: 2015, Volume and Issue: 7, P. 51 - 57

Published: Jan. 14, 2015

Language: Английский

Citations

119
A Neural Network Underlying Circadian Entrainment and Photoperiodic Adjustment of Sleep and Activity in Drosophila DOI Open Access
Margaret L. Schlichting, Pamela Menegazzi,

Katherine R. Lelito

et al.

Journal of Neuroscience, Journal Year: 2016, Volume and Issue: 36(35), P. 9084 - 9096

Published: Aug. 31, 2016

A sensitivity of the circadian clock to light/dark cycles ensures that biological rhythms maintain optimal phase relationships with external day. In animals, neuron network (CCNN) driving sleep/activity receives light input from multiple photoreceptors, but how these photoreceptors modulate CCNN components is not well understood. Here we show Hofbauer-Buchner eyelets differentially two classes ventral lateral neurons (LNvs) within Drosophila CCNN. The antagonize Cryptochrome (CRY)- and compound-eye-based photoreception in large LNvs while synergizing CRY-mediated small LNvs. Furthermore, interact subsets “evening cells” adjust timing evening peak activity a day length-dependent manner. Our work identifies peptidergic connection between group cells critical for seasonal adjustment rhythms. SIGNIFICANCE STATEMENT clocks have evolved orchestrate behavior metabolism. Consistent requires entrainment solar day, process an organism9s health. Light are most important cue clocks, system uses link timekeeping cycle. How information photorecptors integrated into support results establish HB impacts physiology neuronal subgroups. This pathway, together compound eyes, precisely times flies under long summer days. provide mechanistic model transduction integration system, identifying new unexpected motifs network.

Language: Английский

Citations

119
PDF neuron firing phase-shifts key circadian activity neurons in Drosophila DOI Creative Commons
Fang Guo,

Isadora Cerullo,

Chen Xiao

et al.

eLife, Journal Year: 2014, Volume and Issue: 3

Published: June 17, 2014

Our experiments address two long-standing models for the function of Drosophila brain circadian network: a dual oscillator model, which emphasizes primacy PDF-containing neurons, and cell-autonomous model phase adjustment. We identify five different (E) neurons that are major source rhythmicity locomotor activity. Brief firing PDF cells at times day generates response curve (PRC), mimics light-mediated PRC requires receptor expression in E neurons. Firing also resembles light by causing TIM degradation downstream Unlike however, firing-mediated phase-shifting is CRY-independent exploits E3 ligase component CUL-3 early night to degrade TIM. results suggest integrate information then modulate cell oscillations behavioral rhythms. The explain how fly rhythms persist constant darkness without CRY.

Language: Английский

Citations

110