Calcium Imaging and Electrophysiology of hippocampal Activity under Anesthesia and natural Sleep in Mice DOI Creative Commons
A. Formozov, Mattia Chini, Alexander Dieter

et al.

Scientific Data, Journal Year: 2022, Volume and Issue: 9(1)

Published: March 29, 2022

Abstract The acute effects of anesthesia and their underlying mechanisms are still not fully understood. Thus, comprehensive analysis efficient generalization require description in various brain regions. Here we describe a large-scale, annotated collection 2-photon calcium imaging data multi-electrode, extracellular electrophysiological recordings CA1 the murine hippocampus under three distinct anesthetics (Isoflurane, Ketamine/Xylazine Medetomidine/Midazolam/Fentanyl), during natural sleep, wakefulness. We cover several aspects quality standardization provide set tools for autonomous validation, along with workflows reuse exploration. datasets described here capture neural activity hundreds pyramidal cells at single cell resolution. In addition to relevance basic biological research, dataset may find utility computational neuroscience as benchmark models sleep.

Language: Английский

An increase of inhibition drives the developmental decorrelation of neural activity DOI Creative Commons
Mattia Chini, Thomas Pfeffer, Ileana L. Hanganu‐Opatz

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Aug. 17, 2022

Throughout development, the brain transits from early highly synchronous activity patterns to a mature state with sparse and decorrelated neural activity, yet mechanisms underlying this process are poorly understood. The developmental transition has important functional consequences, as latter is thought allow for more efficient storage, retrieval, processing of information. Here, we show that, in mouse medial prefrontal cortex (mPFC), during first two postnatal weeks decorrelates following specific spatial patterns. This accompanied by concomitant tilting excitation-inhibition (E-I) ratio toward inhibition. Using optogenetic manipulations network modeling, that phenomena mechanistically linked, relative increase inhibition drives decorrelation activity. Accordingly, mice mimicking etiology neurodevelopmental disorders, subtle alterations E-I associated impairments correlational structure spike trains. Finally, capitalizing on EEG data newborn babies, an analogous takes place also human brain. Thus, changes control (de)correlation and, these means, its imbalance might contribute pathogenesis disorders.

Language: Английский

Citations

118
Sensitive multicolor indicators for monitoring norepinephrine in vivo DOI
Zacharoula Kagiampaki, Valentin Lu Rohner, Cedric Kiss

et al.

Nature Methods, Journal Year: 2023, Volume and Issue: 20(9), P. 1426 - 1436

Published: July 20, 2023

Language: Английский

Citations

47
Centripetal integration of past events in hippocampal astrocytes regulated by locus coeruleus DOI Creative Commons
Peter Rupprecht, Sian N. Duss, Denise Becker

et al.

Nature Neuroscience, Journal Year: 2024, Volume and Issue: 27(5), P. 927 - 939

Published: April 3, 2024

Abstract An essential feature of neurons is their ability to centrally integrate information from dendrites. The activity astrocytes, in contrast, has been described as mostly uncoordinated across cellular compartments without clear central integration. Here we report conditional integration calcium signals astrocytic distal processes at soma. In the hippocampus adult mice both sexes, found that global activity, recorded with population imaging, reflected past neuronal and behavioral events on a timescale seconds. Salient events, indicated by pupil dilations, facilitated propagation Centripetal soma was reproduced optogenetic activation locus coeruleus, key regulator arousal, reduced pharmacological inhibition α1-adrenergic receptors. Together, our results suggest astrocytes are computational units brain slowly conditionally upon behaviorally relevant events.

Language: Английский

Citations

27
Microglial motility is modulated by neuronal activity and correlates with dendritic spine plasticity in the hippocampus of awake mice DOI Creative Commons
Felix C. Nebeling, Stefanie Poll,

Lena Christine Justus

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 7, 2023

Microglia, the resident immune cells of brain, play a complex role in health and disease. They actively survey brain parenchyma by physically interacting with other structurally shaping brain. Yet, mechanisms underlying microglial motility significance for synapse stability, especially hippocampus during adulthood, remain widely unresolved. Here, we investigated effect neuronal activity on implications formation survival dendritic spines hippocampal CA1 neurons vivo. We used repetitive two-photon vivo imaging awake anesthetized mice to simultaneously study microglia their interaction spines. found that CA3 input is sufficient modulate process motility. Simultaneously, more emerged after compared imaging. Interestingly, rate contacts individual dendrites was associated removal, emergence These results suggest might sense via neurotransmitter release participate synaptic rewiring neural network adulthood. Further, this has profound relevance learning memory processes.

Language: Английский

Citations

32
Multiphoton intravital microscopy of rodents DOI
Colinda L. G. J. Scheele, David Herrmann, Erika Yamashita

et al.

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: Nov. 10, 2022

Language: Английский

Citations

37
Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy DOI Creative Commons

Waja Wegner,

Heinz Steffens, Carola Gregor

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Feb. 23, 2022

Synaptic plasticity underlies long-lasting structural and functional changes to brain circuitry its experience-dependent remodeling can be fundamentally enhanced by environmental enrichment. It is however unknown, whether how the enrichment alters morphology dynamics of individual synapses. Here, we present a virtually crosstalk-free two-color in vivo stimulated emission depletion (STED) microscope simultaneously superresolve endogenous PSD95 post-synaptic density spine geometry mouse cortex. In general, head assemblies were highly dynamic, their depended linearly on original size but correlated only mildly. With enrichment, distributions sizes sharper than controls, indicating that synaptic strength set more uniformly. The topography nanoorganization was dynamic after enrichment; smaller mice housed standard cages. Thus, time-lapse imaging uncovers unique nanoplasticity associated with learning capabilities under

Language: Английский

Citations

22
Divergent Neural Activity in the VLPO During Anesthesia and Sleep DOI Creative Commons

Mengqiang Luo,

Xiang Fei,

Xiaotong Liu

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(2)

Published: Dec. 3, 2022

Abstract The invention of general anesthesia (GA) represents a significant advance in modern clinical practices. However, the exact mechanisms GA are not entirely understood. Because multitude similarities between and sleep, one intriguing hypothesis is that may engage sleep‐wake regulation circuits. Here, using fiber photometry micro‐endoscopic imaging Ca 2+ signals at both population single‐cell levels, it investigates how various anesthetics modulate neural activity ventrolateral preoptic nucleus (vLPO), brain region essential for initiation sleep. It found different primarily induced suppression tended to recruit similar group vLPO neurons; however, each anesthetic caused comparable modulations wake‐active sleep‐active neurons. These results demonstrate creates state than during natural suggesting same circuits sleep generation.

Language: Английский

Citations

22
Efficacy of Tricaine (MS-222) and Hypothermia as Anesthetic Agents for Blocking Sensorimotor Responses in Larval Zebrafish DOI Creative Commons

Claire Leyden,

Timo Brüggemann,

Florentyna Debinski

et al.

Frontiers in Veterinary Science, Journal Year: 2022, Volume and Issue: 9

Published: March 28, 2022

Tricaine, or MS-222, is the most commonly used chemical anesthetic in zebrafish research. It thought to act via blocking voltage-gated sodium channels, though its mechanism of action, particularly at neuronal level, not yet fully understood. Here, we first characterized effects tricaine on both body balance and touch responses freely swimming animals, before determining effect neural activity underlying optokinetic response level motion perception, sensorimotor signaling generation behavior immobilized animals. We found that standard dose for larvae (168 mg/L) induced loss righting reflex within 30 seconds, which then recovered 3 minutes. Optokinetic 15 Calcium imaging showed interferes with by interruption signals between pretectum hindbrain. The sensitivity indices identified sensory neurons were unchanged exposed tricaine, fewer such detected, leaving a small population active neurons. compared gradual cooling, potential non-chemical alternative method anesthesia. While tuning appeared be affected similar manner during cooling surge calcium levels This surge, alongside drop heartrate, potentially associated harmful changes physiology suggests better agent than laboratory

Language: Английский

Citations

21
Machines, mathematics, and modules: the potential to provide real-time metrics for pain under anesthesia DOI Creative Commons
Ke Peng, Keerthana Deepti Karunakaran, Stephen Green

et al.

Neurophotonics, Journal Year: 2024, Volume and Issue: 11(01)

Published: Feb. 22, 2024

The brain-based assessments under anesthesia have provided the ability to evaluate pain/nociception during surgery and potential prevent long-term evolution of chronic pain. Prior studies shown that functional near-infrared spectroscopy (fNIRS)-measured changes in cortical regions such as primary somatosensory polar frontal cortices show consistent response evoked ongoing pain awake, sedated, anesthetized patients. We take this basic approach integrate it into a framework could provide real-time measures peri-surgical period. This application significant implications for providing analgesia surgery, practice currently lacks quantitative evidence guide patient tailored management. Through simple readout “pain” or “no pain,” proposed system diminish eliminate levels intraoperative, early post-operative, potentially, transition post-surgical system, when validated, also be applied analgesic efficacy clinic.

Language: Английский

Citations

4
Instability of excitatory synapses in experimental autoimmune encephalomyelitis and the outcome for excitatory circuit inputs to individual cortical neurons DOI
Rebecca L. Gillani, Eseza N. Kironde,

Sara Whiteman

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 119, P. 251 - 260

Published: March 27, 2024

Language: Английский

Citations

4