Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms

Antibiotics, Journal Year: 2023, Volume and Issue: 12(2), P. 328 - 328

Published: Feb. 4, 2023

Most bacteria attach to biotic or abiotic surfaces and are embedded in a complex matrix which is known as biofilm. Biofilm formation especially worrisome clinical settings it hinders the treatment of infections with antibiotics due facilitated acquisition antibiotic resistance genes (ARGs). Environmental now considered pivotal for driving biofilm formation, biofilm-mediated development dissemination. Several studies have demonstrated that environmental biofilms can be hotspots dissemination ARGs. These encoded on mobile genetic elements (MGEs) such conjugative mobilizable plasmids integrative (ICEs). ARGs rapidly transferred through horizontal gene transfer (HGT) has been shown occur more frequently than planktonic cultures. models promising tools mimic natural study via HGT. This review summarizes state-of-the-art techniques visualize three main HGT mechanisms biofilms: transformation, transduction, conjugation.

Language: Английский

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IntegronFinder 2.0: Identification and Analysis of Integrons across Bacteria, with a Focus on Antibiotic Resistance in Klebsiella

Microorganisms, Journal Year: 2022, Volume and Issue: 10(4), P. 700 - 700

Published: March 24, 2022

Integrons are flexible gene-exchanging platforms that contain multiple cassettes encoding accessory genes whose order is shuffled by a specific integrase. embedded within mobile genetic elements often antibiotic resistance they spread among nosocomial pathogens and contribute to the current crisis. However, most integrons presumably sedentary encode much broader diversity of functions. IntegronFinder widely used software identify novel in bacterial genomes, but has aged lacks some useful functionalities handle very large datasets draft genomes or metagenomes. Here, we present version 2. We have updated code, improved its efficiency usability, adapted output incomplete genome data, added few describe these changes illustrate relevance program analyzing distribution across more than 20,000 fully sequenced genomes. also take full advantage capabilities analyze close 4000 Klebsiella pneumoniae for presence them. Our data show K. largest integron our database plasmids. The pangenome contains total 165 different gene families with being related numerous types antibiotics. free open-source available on public platforms.

Language: Английский

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Selfish, promiscuous and sometimes useful: how mobile genetic elements drive horizontal gene transfer in microbial populations

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 377(1861)

Published: Aug. 22, 2022

Horizontal gene transfer (HGT) drives microbial adaptation but is often under the control of mobile genetic elements (MGEs) whose interests are not necessarily aligned with those their hosts. In general, costly to donor cell while potentially beneficial recipients. The diversity and plasticity cell-MGEs interactions, among MGEs, result in complex evolutionary processes where source, or even existence selection for maintaining a function genome, unclear. For example, MGE-driven HGT depends on envelope structures defense systems, many these transferred by MGEs themselves. can spur periods intense increasing own rates horizontal transmission upon communicating, eavesdropping, sensing environment host physiology. This may high-frequency genes unrelated MGE. Here, we review how drive mechanisms, selective pressures genomic traits affect flow, therefore adaptation, populations. encoding adaptive niche-defining means that intragenomic conflicts alliances between cells key functional diversification. article part discussion meeting issue 'Genomic population pathogens'.

Language: Английский

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How do interactions between mobile genetic elements affect horizontal gene transfer?

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 73, P. 102282 - 102282

Published: Feb. 28, 2023

Horizontal gene transfer is central to bacterial adaptation and facilitated by mobile genetic elements (MGEs). Increasingly, MGEs are being studied as agents with their own interests adaptations, the interactions have one another recognised having a powerful effect on flow of traits between microbes. Collaborations conflicts nuanced can both promote inhibit acquisition new material, shaping maintenance newly acquired genes dissemination important adaptive through microbiomes. We review recent studies that shed light this dynamic oftentimes interlaced interplay, highlighting importance genome defence systems in mediating MGE-MGE conflicts, outlining consequences for evolutionary change, resonate from molecular microbiome ecosystem levels.

Language: Английский

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Carbapenem-resistant Klebsiella pneumoniae capsular types, antibiotic resistance and virulence factors in China: a longitudinal, multi-centre study

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(3), P. 814 - 829

Published: Feb. 29, 2024

Abstract Epidemiological knowledge of circulating carbapenem-resistant Klebsiella pneumoniae (CRKP) is needed to develop effective strategies against this public health threat. Here we present a longitudinal analysis 1,017 CRKP isolates recovered from patients 40 hospitals across China between 2016 and 2020. Virulence gene capsule typing revealed expansion type KL64 (59.5%) alongside decreases in KL47 prevalence. Hypervirulent increased prevalence 28.2% 45.7% Phylogenetic spatiotemporal Beijing Shanghai as transmission hubs accounting for differential geographical strains China. Moderate frequency or O-antigen loss was also detected among isolates. Non-capsular were more susceptible phagocytosis, attenuated during mouse infections, but showed serum resistance biofilm formation. These findings give insight into serotype dynamics, revealing the importance monitoring shifts future development immunological infections.

Language: Английский

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Genetic determinants of host tropism in Klebsiella phages

Cell Reports, Journal Year: 2023, Volume and Issue: 42(2), P. 112048 - 112048

Published: Feb. 1, 2023

Bacteriophages play key roles in bacterial ecology and evolution are potential antimicrobials. However, the determinants of phage-host specificity remain elusive. Here, we isolate 46 phages to challenge 138 representative clinical isolates Klebsiella pneumoniae, a widespread opportunistic pathogen. Spot tests show narrow host range for most phages, with <2% 6,319 combinations tested yielding detectable interactions. Bacterial capsule diversity is main factor restricting phage range. Consequently, phage-encoded depolymerases tropism, depolymerase sequence types associated ability infect specific capsular across families. all broader found do not encode canonical depolymerases, suggesting alternative modes entry. These findings expand our knowledge complex interactions between bacteria their viruses point out feasibility predicting first steps infection using genome sequences.

Language: Английский

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Bacterial capsules: Occurrence, mechanism, and function

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: March 13, 2024

Abstract In environments characterized by extended multi-stress conditions, pathogens develop a variety of immune escape mechanisms to enhance their ability infect the host. The capsules, polymers that bacteria secrete near cell wall, participates in numerous bacterial life processes and plays crucial role resisting host attacks adapting niche. Here, we discuss relationship between capsules virulence, summarizing molecular capsular regulation pathogenesis provide new insights into research on pathogenic bacteria.

Language: Английский

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Prediction of strain level phage–host interactions across the Escherichia genus using only genomic information

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(11), P. 2847 - 2861

Published: Oct. 31, 2024

Language: Английский

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Common and distinctive genomic features of Klebsiella pneumoniae thriving in the natural environment or in clinical settings

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: June 21, 2022

Abstract The Klebsiella pneumoniae complex is comprised of ubiquitous bacteria that can be found in soils, plants or water, and as humans’ opportunistic pathogens. This study aimed at inferring common distinctive features clinical environmental K. pneumoniae. Whole genome sequences members the (including variicola , n = 6; quasipneumoniae 7), (n 78) 61) origin from 21 countries were accessed GenBank. These genomes compared based on phylogeny, pangenome selected clinically relevant traits. Phylogenetic analysis 2704 genes core showed close relatedness between strains, agreement with multi-locus sequence typing. Eight out 62 types (STs) identified, included both (ST11, ST14, ST15, ST37, ST45, ST147, ST348, ST437). Pangenome-wide association studies did not evidence significant differences genomes. However, isolates presented significantly more exclusive related to antibiotic resistance/plasmids, while yielded higher allelic diversity functions such efflux oxidative stress. suggests circulate among natural environment settings, probably under distinct adaptation pressures.

Language: Английский

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Host-specific plasmid evolution explains the variable spread of clinical antibiotic-resistance plasmids

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(15)

Published: April 6, 2023

Antibiotic resistance encoded on plasmids is a pressing global health problem. Predicting which spread in the long term remains very challenging, even though some key parameters influencing plasmid stability have been identified, such as growth costs and horizontal transfer rates. Here, we show these evolve strain-specific way among clinical bacteria, this occurs rapidly enough to alter relative likelihoods of different bacterium–plasmid combinations spreading. We used experiments with Escherichia coli antibiotic-resistance isolated from patients, paired mathematical model, track long-term (beyond antibiotic exposure). Explaining variable across six required accounting for evolutionary changes traits, whereas initial variation was relatively poor predictor outcomes. Evolutionary trajectories were specific particular combinations, evidenced by genome sequencing genetic manipulation. This revealed epistatic (here, strain-dependent) effects affecting transfer. Several involved mobile elements pathogenicity islands. Rapid evolution can thus outweigh ancestral phenotypes stability. Accounting natural populations could improve our ability anticipate manage successful combinations.

Language: Английский

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