Phage-Plasmids Spread Antibiotic Resistance Genes through Infection and Lysogenic Conversion

mBio, Journal Year: 2022, Volume and Issue: 13(5)

Published: Sept. 26, 2022

Antibiotic resistance is rapidly spreading via the horizontal transfer of genes in mobile genetic elements. While plasmids are key drivers this process, few integrative phages encode antibiotic genes. Here, we find that phage-plasmids, elements both and plasmids, often carry We found 60 phage-plasmids with 184 genes, providing for broad-spectrum-cephalosporins, carbapenems, aminoglycosides, fluoroquinolones, colistin. These a hot spots, seem to have been cotranslocated transposable elements, class I integrons, which had not previously phages. tried induce six (including four integrons) succeeded five cases. Other prophages were coinduced these experiments. As proof concept, focused on P1-like element encoding an extended spectrum β-lactamase,

Language: Английский

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Microbial evolution through horizontal gene transfer by mobile genetic elements

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(1)

Published: Jan. 1, 2024

Mobile genetic elements (MGEs) are crucial for horizontal gene transfer (HGT) in bacteria and facilitate their rapid evolution adaptation. MGEs include plasmids, integrative conjugative elements, transposons, insertion sequences bacteriophages. Notably, the spread of antimicrobial resistance genes (ARGs), which poses a serious threat to public health, is primarily attributable HGT through MGEs. This mini-review aims provide an overview mechanisms by mediate microbes. Specifically, behaviour plasmids different environments conditions was discussed, recent methodologies tracing dynamics were summarised. A comprehensive understanding underlying role bacterial adaptation important develop strategies combat ARGs.

Language: Английский

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The defensome of complex bacterial communities

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 8, 2024

Abstract Bacteria have developed various defense mechanisms to avoid infection and killing in response the fast evolution turnover of viruses other genetic parasites. Such pan-immune system ( defensome ) encompasses a growing number lines that include well-studied innate adaptive systems such as restriction-modification, CRISPR-Cas abortive infection, but also newly found ones whose are still poorly understood. While abundance distribution is well-known complete culturable genomes, there void our understanding their diversity richness complex microbial communities. Here we performed large-scale in-depth analysis defensomes 7759 high-quality bacterial population genomes reconstructed from soil, marine, human gut environments. We observed wide variation frequency nature among large phyla, which correlated with lifestyle, genome size, habitat, geographic background. The defensome’s mobility, its clustering islands, variability was be system-specific shaped by environment. Hence, results provide detailed picture multiple immune barriers present environmentally distinct communities set stage for subsequent identification novel ingenious strategies diversification uncultivated microbes.

Language: Английский

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Comprehensive genomic insights into a highly pathogenic clone ST656 of mcr8.1 containing multidrug-resistant Klebsiella pneumoniae from Bangladesh

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 18, 2025

Language: Английский

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Profile and resistance levels of 136 integron resistance genes

npj Antimicrobials and Resistance, Journal Year: 2023, Volume and Issue: 1(1)

Published: Oct. 11, 2023

Integrons have played a major role in the rise and spread of multidrug resistance Gram-negative pathogens are nowadays commonplace among clinical isolates. These platforms capture, stockpile, modulate expression more than 170 antimicrobial cassettes (ARCs) against most clinically-relevant antibiotics. Despite their importance, our knowledge on profile levels is patchy, because data scattered literature, often reported different genetic backgrounds sometimes extrapolated from sequence similarity alone. Here we generated collection 136 ARCs 8 antibiotic families disinfectants. Cassettes cloned vector designed to mimic environment class 1 integron, transformed Escherichia coli. We measured minimal inhibitory concentration (MIC) relevant molecules each family. With 500 MIC values, provide an exhaustive comparable quantitation conferred by ARCs. Our confirm known trends profiles while revealing important differences closely related genes. also detected genes that do not confer expected resistance, point challenging whole family qac work provides detailed characterization integron at-a-glance.

Language: Английский

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Population structure and antibiotic resistance of swine extraintestinal pathogenic Escherichia coli from China

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 10, 2024

Abstract Extraintestinal Pathogenic Escherichia coli (ExPEC) pose a significant threat to human and animal health. However, the diversity antibiotic resistance of ExPEC, their connection infections, remain largely unexplored. The study performs large-scale genome sequencing testing 499 swine-derived ExPEC isolates from China. Results show swine are phylogenetically diverse, with over 80% belonging phylogroups B1 A. Importantly, 15 exhibit genetic relatedness human-origin E. strains. Additionally, 49 strains harbor toxins typical enteric pathotypes, implying hybrid pathotypes. Notably, 97% total multidrug resistant, including critical drugs like third- fourth-generation cephalosporins. Correspondingly, genomic analysis unveils prevalent genes (ARGs), often associated co-transfer mechanisms. Furthermore, 20 complete genomes illuminates transmission pathways ARGs within pathogens. For example, plasmids co-harboring fosA3 , bla CTX-M-14 mcr-1 between Salmonella enterica is observed. These findings underscore importance monitoring controlling infections in animals, as they can serve reservoir potential affect health or even be origin pathogens infecting humans.

Language: Английский

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Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(1), P. 228 - 240

Published: Jan. 3, 2024

Language: Английский

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Nationwide genome surveillance of carbapenem-resistant Pseudomonas aeruginosa in Japan

Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: 68(5)

Published: April 2, 2024

Japan is a country with an approximate 10% prevalence rate of carbapenem-resistant

Language: Английский

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Carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae with a highly transmissible dual-carbapenemase plasmid in Chile

Biological Research, Journal Year: 2024, Volume and Issue: 57(1)

Published: March 12, 2024

Abstract Background The convergence of hypervirulence and carbapenem resistance in the bacterial pathogen Klebsiella pneumoniae represents a critical global health concern. Hypervirulent K. (hvKp) strains, frequently from sequence type 23 (ST23) having K1 capsule, have been associated with severe community-acquired invasive infections. Although hvKp were initially restricted to Southeast Asia primarily antibiotic-sensitive, carbapenem-resistant infections are reported worldwide. Here, within carbapenemase production Enterobacterales surveillance system headed by Chilean Public Health Institute, we describe isolation Chile high-risk ST23 dual-carbapenemase-producing strain, which genes encoded single conjugative plasmid. Results Phenotypic molecular tests this strain revealed an extensive at least 15 antibiotic classes KPC-2 VIM-1 carbapenemases. Unexpectedly, isolate lacked hypermucoviscosity, challenging commonly used identification criteria. Complete genome sequencing analysis confirmed capsular type, KpVP-1 virulence plasmid, GIE492 ICEKp10 genomic islands carrying factors strongly hvKp. belonged globally disseminated clonal group CG23-I, it is unique, as formed clade apart previously acquired IncN plasmid highly South America (absent other genomes), but now including class-I integron bla VIM−1 genes. Notably, was able conjugate double E. coli recipient, conferring 1st -5th generation cephalosporins (including combinations beta-lactamase inhibitors), penicillins, monobactams, carbapenems. Conclusions We transmissible encoding carbapenemases, most beta-lactams. Furthermore, lack hypermucoviscosity argues against trait reliable marker. These findings highlight rapid evolution towards multi-drug globally, well importance plasmids mobile genetic elements convergence. In regard, approaches provide valuable support monitor obtain essential information on these priority pathogens elements.

Language: Английский

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Genomic resistance in historical clinical isolates increased in frequency and mobility after the age of antibiotics

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Abstract Antibiotic resistance is frequently observed shortly after the clinical introduction of an antibiotic. Whether and how that occurred before harder to determine, as isolates could not have been tested for antibiotic was discovered. Historical collections, like British National Collection Type Cultures (NCTC), stretching back 1885, provide a window into this history. Here we match 1,817 sequenced high-quality genomes from NCTC collection their respective year isolation study genes concurrent with age antibiotics. Concordant previous work, find in both pathogens environmental samples While generally rare antibiotic, associated increase frequency introduction. Finally, observe trend elements becoming increasingly mobile nested within multiple time goes on. More broadly, our findings suggest likely-functional were circulating clinically relevant antibiotics, but human usage increasing overall prevalence mobility. DATA SUMMARY Genome assemblies downloaded analyzed are Supplementary Table 1, computational tools used found Methods. The authors confirm all supporting data, code protocols provided article or through supplementary data files. Impact statement collections microbial enable researchers investigate past identify interesting trends over time. In study, queried 1,800 isolate one such genomic variation linked resistance. We show numerous cultured given contain resistance; however, phenomenon remained relatively rare. demonstrate strong association between introduced rise while common throughout timeframe analyzed, has become gone This shows expected, correlated also increased mobility alleles conferring However, note effect deposition bias cannot be excluded. Our work indicates pangenomes naturally contained even anthropogenic use Taken together, although may affect isolates; most existed sampled prior Quantifying understanding impact helps us understand novel antibiotics can bacteria; allowing better reaction resistant infections they arise.

Language: Английский

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