Single-neuron perturbations reveal feature-specific competition in V1

Nature, Journal Year: 2019, Volume and Issue: 567(7748), P. 334 - 340

Published: March 6, 2019

Language: Английский

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The Spatiotemporal Organization of the Striatum Encodes Action Space

Neuron, Journal Year: 2017, Volume and Issue: 96(4), P. 949 - 949

Published: Nov. 1, 2017

Language: Английский

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Making sense of astrocytic calcium signals — from acquisition to interpretation

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 21(10), P. 551 - 564

Published: Sept. 1, 2020

Language: Английский

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Robustness of Spike Deconvolution for Neuronal Calcium Imaging

Journal of Neuroscience, Journal Year: 2018, Volume and Issue: 38(37), P. 7976 - 7985

Published: Aug. 6, 2018

Calcium imaging is a powerful method to record the activity of neural populations in many species, but inferring spike times from calcium signals challenging problem. We compared multiple approaches using datasets with ground truth electrophysiology and found that simple non-negative deconvolution (NND) outperformed all other algorithms on out-of-sample test data. introduce novel benchmark applicable recordings without electrophysiological truth, based correlation responses two stimulus repeats, used this show unconstrained NND also when run “zoomed out” ∼10,000 cell visual cortex mice either sex. Finally, we NND-based methods match performance supervised convolutional networks while avoiding some biases such methods, at much faster running times. therefore recommend spikes be inferred traces because its simplicity, efficiency, accuracy. SIGNIFICANCE STATEMENT The experimental currently allows for largest numbers cells simultaneously two-photon imaging. However, use requires neuronal firing correctly large resulting datasets. Previous studies have claimed complex learning outperform task. Unfortunately, these suffered several problems biases. When repeated analysis, same data correcting problems, simpler inference perform better. Even more importantly, can artifactual structure into trains, which turn lead erroneous scientific conclusions. Of evaluated, an extremely performed best circumstances tested, was run, insensitive parameter choices, making incorrect conclusions less likely.

Language: Английский

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Community-based benchmarking improves spike rate inference from two-photon calcium imaging data

PLoS Computational Biology, Journal Year: 2018, Volume and Issue: 14(5), P. e1006157 - e1006157

Published: May 21, 2018

In recent years, two-photon calcium imaging has become a standard tool to probe the function of neural circuits and study computations in neuronal populations. However, acquired signal is only an indirect measurement activity due comparatively slow dynamics fluorescent indicators. Different algorithms for estimating spike rates from noisy measurements have been proposed past, but it open question how far performance can be improved. Here, we report results spikefinder challenge, launched catalyze development new rate inference through crowd-sourcing. We present ten submitted which show improved compared previously evaluated methods. Interestingly, top-performing are based on wide range principles deep networks generative models, yet provide highly correlated estimates activity. The competition shows that benchmark challenges drive algorithmic developments neuroscience.

Language: Английский

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Kilohertz frame-rate two-photon tomography

Nature Methods, Journal Year: 2019, Volume and Issue: 16(8), P. 778 - 786

Published: July 29, 2019

Language: Английский

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Single-cell activity tracking reveals that orbitofrontal neurons acquire and maintain a long-term memory to guide behavioral adaptation

Nature Neuroscience, Journal Year: 2019, Volume and Issue: 22(7), P. 1110 - 1121

Published: June 3, 2019

Language: Английский

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Reconstruction of neocortex: Organelles, compartments, cells, circuits, and activity

Cell, Journal Year: 2022, Volume and Issue: 185(6), P. 1082 - 1100.e24

Published: Feb. 24, 2022

Language: Английский

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Dysfunction of cortical GABAergic neurons leads to sensory hyper-reactivity in a Shank3 mouse model of ASD

Nature Neuroscience, Journal Year: 2020, Volume and Issue: 23(4), P. 520 - 532

Published: March 2, 2020

Language: Английский

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Relationship between simultaneously recorded spiking activity and fluorescence signal in GCaMP6 transgenic mice

eLife, Journal Year: 2021, Volume and Issue: 10

Published: March 8, 2021

Fluorescent calcium indicators are often used to investigate neural dynamics, but the relationship between fluorescence and action potentials (APs) remains unclear. Most APs can be detected when soma almost fills microscope's field of view, image populations neurons, necessitating a large generating fewer photons per neuron, compromising AP detection. Here, we characterized AP-fluorescence transfer function in vivo for 48 layer 2/3 pyramidal neurons primary visual cortex, with simultaneous imaging cell-attached recordings from transgenic mice expressing GCaMP6s or GCaMP6f. While most were under optimal conditions, conditions typical population studies, only minority 1 2 events (often <10% ~20-30%, respectively), emphasizing limits detection more realistic conditions.Neurons, cells that make up nervous system, transmit information using electrical signals known as spikes. Studying spiking patterns brain is essential understand perception, memory, thought, behaviour. One way do by recording activity microelectrodes. Another study neuronal molecules change how they interact light binds them, since changes concentration indicative spiking. That observed specialized microscopes know two-photon microscopes. Using indicators, it possible simultaneously record hundreds even thousands neurons. However, spikes not translate one-to-one. In order interpret data, important associated individual The directly measure this same neuron. extremely challenging experimentally, so type data rare. To shed some on this, Huang, Ledochowitsch et al. had been genetically modified produce indicator cortex obtained both measurements these These experiments revealed that, while majority time periods containing multi-spike could identified microscopy, average, less than 10% isolated single detectable. This an caveat researchers need take into consideration interpreting results. findings intended serve guide studies look at mammalian level. addition, provided will useful reference development sensors, benchmark improve computational approaches detecting predicting

Language: Английский

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