VIP interneurons regulate cortical size tuning and visual perception DOI Creative Commons
Katie Ferguson,

Jenna Salameh,

Christopher Alba

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(9), P. 113088 - 113088

Published: Sept. 1, 2023

Cortical circuit function is regulated by extensively interconnected, diverse populations of GABAergic interneurons that may play key roles in shaping operation according to behavioral context. A specialized population co-express vasoactive intestinal peptides (VIP-INs) are activated during arousal and innervate other INs pyramidal neurons (PNs). Although state-dependent modulation VIP-INs has been studied, their role regulating sensory processing less well understood. We examined the impact primary visual cortex awake behaving mice. Loss VIP-IN activity alters somatostatin-expressing (SST-INs) but not PNs. In contrast, reduced globally disrupts feature selectivity for stimulus size. Moreover, on perceptual behavior varies with context more acute small than large cues. thus contribute both cortical context-dependent performance.

Language: Английский

Fast and sensitive GCaMP calcium indicators for imaging neural populations DOI Creative Commons
Yan Zhang,

Márton Rózsa,

Yajie Liang

et al.

Nature, Journal Year: 2023, Volume and Issue: 615(7954), P. 884 - 891

Published: March 15, 2023

Abstract Calcium imaging with protein-based indicators 1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report at timescales much slower than electrical signalling and are limited by trade-offs between sensitivity kinetics. Here we large-scale screening structure-guided mutagenesis develop optimize several fast sensitive GCaMP-type 3–8 . The resulting ‘jGCaMP8’ sensors, based on the calcium-binding calmodulin a fragment of endothelial nitric oxide synthase, have ultra-fast kinetics (half-rise times 2 ms) highest for reported calcium sensor. jGCaMP8 will allow tracking large populations neurons relevant computation.

Language: Английский

Citations

455
Beyond t test and ANOVA: applications of mixed-effects models for more rigorous statistical analysis in neuroscience research DOI Creative Commons
Zhaoxia Yu, Michele Guindani, Steven F. Grieco

et al.

Neuron, Journal Year: 2021, Volume and Issue: 110(1), P. 21 - 35

Published: Nov. 15, 2021

Language: Английский

Citations

364
Prefrontal Cortex Development in Health and Disease: Lessons from Rodents and Humans DOI Creative Commons
Mattia Chini, Ileana L. Hanganu‐Opatz

Trends in Neurosciences, Journal Year: 2020, Volume and Issue: 44(3), P. 227 - 240

Published: Nov. 24, 2020

The role of the prefrontal cortex (PFC) takes center stage among unanswered questions in modern neuroscience. PFC has a Janus-faced nature: it enables sophisticated cognitive and social abilities that reach their maximum expression humans, yet underlies some devastating symptoms psychiatric disorders. Accordingly, appropriate development is crucial for many high-order dysregulation this process been linked to various neuropsychiatric diseases. Reviewing recent advances field, with primary focus on rodents we highlight why, despite differences across species, cross-species approach fruitful strategy understanding development. We briefly review developmental contribution molecules extensively discuss how electrical activity controls early maturation wiring areas, as well emergence refinement input-output circuitry involved processing. Finally, mechanisms dysfunction relevance

Language: Английский

Citations

193
Representational drift in the mouse visual cortex DOI Creative Commons

Daniel Deitch,

Alon Rubin,

Yaniv Ziv

et al.

Current Biology, Journal Year: 2021, Volume and Issue: 31(19), P. 4327 - 4339.e6

Published: Aug. 24, 2021

Language: Английский

Citations

167
Relationship between simultaneously recorded spiking activity and fluorescence signal in GCaMP6 transgenic mice DOI Creative Commons
Lawrence Huang, Peter Ledochowitsch, Ulf Knoblich

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: March 8, 2021

Fluorescent calcium indicators are often used to investigate neural dynamics, but the relationship between fluorescence and action potentials (APs) remains unclear. Most APs can be detected when soma almost fills microscope's field of view, image populations neurons, necessitating a large generating fewer photons per neuron, compromising AP detection. Here, we characterized AP-fluorescence transfer function in vivo for 48 layer 2/3 pyramidal neurons primary visual cortex, with simultaneous imaging cell-attached recordings from transgenic mice expressing GCaMP6s or GCaMP6f. While most were under optimal conditions, conditions typical population studies, only minority 1 2 events (often <10% ~20-30%, respectively), emphasizing limits detection more realistic conditions.Neurons, cells that make up nervous system, transmit information using electrical signals known as spikes. Studying spiking patterns brain is essential understand perception, memory, thought, behaviour. One way do by recording activity microelectrodes. Another study neuronal molecules change how they interact light binds them, since changes concentration indicative spiking. That observed specialized microscopes know two-photon microscopes. Using indicators, it possible simultaneously record hundreds even thousands neurons. However, spikes not translate one-to-one. In order interpret data, important associated individual The directly measure this same neuron. extremely challenging experimentally, so type data rare. To shed some on this, Huang, Ledochowitsch et al. had been genetically modified produce indicator cortex obtained both measurements these These experiments revealed that, while majority time periods containing multi-spike could identified microscopy, average, less than 10% isolated single detectable. This an caveat researchers need take into consideration interpreting results. findings intended serve guide studies look at mammalian level. addition, provided will useful reference development sensors, benchmark improve computational approaches detecting predicting

Language: Английский

Citations

150
A database and deep learning toolbox for noise-optimized, generalized spike inference from calcium imaging DOI
Peter Rupprecht, Stefano Carta, Adrian Hoffmann

et al.

Nature Neuroscience, Journal Year: 2021, Volume and Issue: 24(9), P. 1324 - 1337

Published: Aug. 2, 2021

Language: Английский

Citations

117
Two-photon calcium imaging of neuronal activity DOI
Christine Grienberger, Andrea Giovannucci, William Zeiger

et al.

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: Sept. 1, 2022

Language: Английский

Citations

80
CCR5 closes the temporal window for memory linking DOI
Yang Shen, Miou Zhou, Denise J. Cai

et al.

Nature, Journal Year: 2022, Volume and Issue: 606(7912), P. 146 - 152

Published: May 25, 2022

Language: Английский

Citations

72
Brain-wide representations of behavior spanning multiple timescales and states in C. elegans DOI Creative Commons

Adam A. Atanas,

Jung Soo Kim, Ziyu Wang

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(19), P. 4134 - 4151.e31

Published: Aug. 21, 2023

Changes in an animal's behavior and internal state are accompanied by widespread changes activity across its brain. However, how neurons the brain encode this is impacted poorly understood. We recorded brain-wide diverse motor programs of freely moving C. elegans built probabilistic models that explain each neuron encodes quantitative behavioral features. By determining identities neurons, we created atlas defined classes connectome behavior. Many have conjunctive representations multiple behaviors. Moreover, although many current actions, others integrate recent actions. behavior, identify these flexible nodes connectome. Our results provide a global map cell types

Language: Английский

Citations

47
The centrality of population-level factors to network computation is demonstrated by a versatile approach for training spiking networks DOI Creative Commons
Brian DePasquale, David Sussillo, L. F. Abbott

et al.

Neuron, Journal Year: 2023, Volume and Issue: 111(5), P. 631 - 649.e10

Published: Jan. 10, 2023

Language: Английский

Citations

45