Single-molecule localization microscopy

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: June 3, 2021

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited and can image biological structures at the molecular scale. In SMLM, individual fluorescent molecules are computationally localized from sequences localizations used to generate super-resolution or time course images, define trajectories. this Primer, we introduce basic principles SMLM before describing main experimental considerations when performing including labelling, sample preparation, hardware requirements acquisition in fixed live cells. We then explain how low-resolution processed reconstruct images and/or extract quantitative information, highlight selection discoveries enabled by closely related methods. discuss some limitations potential artefacts as well ways alleviate them. Finally, present an outlook on advanced promising new developments fast-evolving field SMLM. hope Primer will be useful reference for both newcomers practitioners

Language: Английский

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The Self-Organizing Genome: Principles of Genome Architecture and Function

Cell, Journal Year: 2020, Volume and Issue: 183(1), P. 28 - 45

Published: Sept. 24, 2020

Language: Английский

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Principles of genome folding into topologically associating domains

Science Advances, Journal Year: 2019, Volume and Issue: 5(4)

Published: April 5, 2019

This review discusses the features of TADs across species, and their role in chromosome organization, genome function, evolution.

Language: Английский

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Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin

Cell, Journal Year: 2020, Volume and Issue: 182(6), P. 1641 - 1659.e26

Published: Aug. 20, 2020

Language: Английский

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Visualizing DNA folding and RNA in embryos at single-cell resolution

Nature, Journal Year: 2019, Volume and Issue: 568(7750), P. 49 - 54

Published: March 18, 2019

Language: Английский

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Integrated spatial genomics reveals global architecture of single nuclei

Nature, Journal Year: 2021, Volume and Issue: 590(7845), P. 344 - 350

Published: Jan. 27, 2021

Language: Английский

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Understanding 3D genome organization by multidisciplinary methods

Nature Reviews Molecular Cell Biology, Journal Year: 2021, Volume and Issue: 22(8), P. 511 - 528

Published: May 5, 2021

Language: Английский

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The emerging landscape of spatial profiling technologies

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 23(12), P. 741 - 759

Published: July 20, 2022

Language: Английский

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Principles for Integrative Structural Biology Studies

Cell, Journal Year: 2019, Volume and Issue: 177(6), P. 1384 - 1403

Published: May 1, 2019

Integrative structure determination is a powerful approach to modeling the structures of biological systems based on data produced by multiple experimental and theoretical methods, with implications for our understanding cellular biology drug discovery. This Primer introduces theory methods integrative approaches, emphasizing kinds that can be effectively included in developing models using nuclear pore complex as an example illustrate practice challenges involved. These guidelines are intended aid researcher applying structural their interest thus take advantage this rapidly evolving field.

Language: Английский

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Spatial profiling technologies illuminate the tumor microenvironment

Cancer Cell, Journal Year: 2023, Volume and Issue: 41(3), P. 404 - 420

Published: Feb. 16, 2023

Language: Английский

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