Role of Rab5 early endosomes in regulatingDrosophilagut antibacterial response DOI Creative Commons
Manish Joshi,

Annelise Viallat-Lieutaud,

Julien Royet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: May 4, 2022

Abstract Interactions between prokaryotes and eukaryotes require a dialogue MAMPs PRRs. In Drosophila , bacterial peptidoglycan is detected by PGRP receptors. While the components of signaling cascades activated upon PGN/PGRP interactions are well characterized, little known about subcellular events that translate these early steps into target gene transcription. Using enteric infection model, we show gut-associated bacteria can induce formation intracellular PGRP-LE aggregates which colocalized with endosome marker Rab5. Combining microscopic RNA-seq analysis, demonstrate RNAi inactivation endocytosis pathway in gut affects expression essential regulators NF-κB response leading not only to disruption immune locally but also at systemic level. This work sheds new light on involvement control intestinal Graphical abstract

Language: Английский

Ingestion of Bacillus cereus spores dampens the immune response to favor bacterial persistence DOI Creative Commons
Salma Hachfi, Alexandra Brun‐Barale,

Arnaud Fichant

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 4, 2024

Language: Английский

Citations

7
Microbiota-derived acetylcholine can promote gut motility in Drosophila melanogaster DOI Open Access
Yuka Fujita, Hina Kosakamoto, Fumiaki Obata

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

The gut microbiota is crucial for intestinal health, including gastrointestinal (GI) motility. How commensal bacterial species influence GI motility has not been fully elucidated. A major factor of the contraction promoting propulsive movement orally ingested materials. Here, we developed a method to monitor and quantify contractions in living Drosophila melanogaster larvae. We found that culture medium an isolated strain Lactiplantibacillus plantarum Lsi promoted vivo , which was observed Leuconostoc sp. Leui nor Acetobacter persici Ai medium. To identify bacteria-derived metabolites, performed metabolome analysis media by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Of 66 metabolites detected, some changed species-specific manner. Among them, acetylcholine specifically produced L. plantarum. Feeding exogenous increased frequency contractions, blocked D-tubocurarine, inhibitor nicotinic receptors. In this study, propose mechanism influences This article part theme issue ‘Sculpting microbiome: how host factors determine respond microbial colonization’.

Language: Английский

Citations

6
High-fat diets induce inflammatory IMD/NFκB signaling via gut microbiota remodeling in Drosophila DOI Creative Commons
Jun Wang,

Jiaojiao Gu,

Jianhan Yi

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: April 23, 2024

High-fat diets (HFDs), a prevailing daily dietary style worldwide, induce chronic low-grade inflammation in the central nervous system and peripheral tissues, promoting variety of diseases including pathologies associated with neuroinflammation. However, mechanisms linking HFDs to are not entirely clear. Here, using Drosophila HFD model, we explored mechanism HFD-induced remote tissues. We found that activated IMD/NFκB immune pathway head through remodeling commensal gut bacteria. Removal microbiota abolished such inflammatory response. Further experiments revealed significantly increased abundance Acetobacter malorum gut, re-association this bacterium was sufficient elicit response Mechanistically, produced greater amount peptidoglycan (PGN), well-defined microbial molecular pattern enters circulation remotely activates an Our results thus show trigger mediated by bacterial elicits host

Language: Английский

Citations

4
Bacterial peptidoglycan serves as a critical modulator of the gut-immune-brain axis in Drosophila DOI Creative Commons

Florent Fioriti,

Aline Rifflet, Ivo G. Boneca

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 119, P. 878 - 897

Published: May 6, 2024

Metabolites and compounds derived from gut-associated bacteria can modulate numerous physiological processes in the host, including immunity behavior.Using a model of oral bacterial infection, we previously demonstrated that gut-derived peptidoglycan (PGN), an essential constituent cell envelope, influences female fruit fly egg-laying behavior by activating NF-κB cascade subset brain neurons.These findings underscore PGN as potential mediator communication between gut Drosophila, prompting further investigation into its impact on all cells.Through high-resolution mass spectrometry, now show fragments produced rapidly reach central nervous system.In Addition, employing combination whole-genome transcriptome analyses, comprehensive genetic assays, reporter gene systems, reveal infection triggers dose-dependent immune response perineurial glia, forming continuous outer layer blood-brain barrier.Furthermore, demonstrate persistent PGN-dependent activation glial cells correlates with reduction lifespan early neurological decline.Overall, our establish critical gut-immune-brain axis Drosophila.

Language: Английский

Citations

4
MprF-mediated immune evasion is necessary for Lactiplantibacillus plantarum resilience in the Drosophila gut during inflammation DOI Creative Commons
Aranzazu Arias-Rojas, Adini Qisthi Arifah, Georgia Angelidou

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(8), P. e1012462 - e1012462

Published: Aug. 19, 2024

Multiple peptide resistance factor (MprF) confers to cationic antimicrobial peptides (AMPs) in several pathogens, thereby enabling evasion of the host immune response. The role MprF commensals remains, however, uncharacterized. To close this knowledge gap, we used a common gut commensal animals, Lactiplantibacillus plantarum , and its natural host, fruit fly Drosophila melanogaster as an experimental model investigate commensal-host interactions. L . ΔmprF mutant that generated exhibited deficiency synthesis lysyl-phosphatidylglycerol (Lys-PG), resulting increased negative cell surface charge susceptibility AMPs. Susceptibility AMPs had no effect on mutant’s ability colonize guts uninfected flies. However, observed significantly reduced abundance after infection-induced inflammation wild-type flies but not lacking Additionally, found compared induces stronger intestinal response due release immunostimulatory peptidoglycan fragments, indicating important promoting tolerance commensals. Our further analysis suggests MprF-mediated lipoteichoic acid modifications are involved immunomodulation. Overall, our results demonstrate MprF, besides well-characterized pathogen virulence, is also resilience factor.

Language: Английский

Citations

4
Different Species of Yeast Alter the Lifespan and Fecundity of Drosophila melanogaster DOI
Ekaterina Yakovleva,

I. A. Maximova,

D. S. Merzlikin

et al.

Biology Bulletin Reviews, Journal Year: 2025, Volume and Issue: 15(1), P. 109 - 128

Published: Feb. 1, 2025

Language: Английский

Citations

0
Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies DOI Creative Commons

Arnau Rocabert,

Joan Martín-Pérez,

Laia Pareras

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 980, P. 179545 - 179545

Published: April 30, 2025

Micro- and nanoplastics (MNPLs) are emerging environmental pollutants that have garnered significant attention over the past few decades due to their detrimental effects on human health through various exposure pathways. This study investigates impact of MNPLs gut microbiota, utilizing Drosophila melanogaster as a model organism. was selected for its microbiota's similarities humans established role an accessible well-characterized system. To analyze full-length 16S rRNA gene sequencing performed using Nanopore platform, enabling comprehensive profiling microbial populations present in samples. As models MNPLs, two commercial polystyrene (PS-NPLs, 61.20 415.22 nm) one lab-made polylactic acid nanoplastic (PLA-NPLs, 463.90 were selected. positive control, zinc oxide nanoparticles (ZnO-NPs) used. The observed findings revealed induced notable alterations including reduction bacterial abundance shifts species composition. These results suggest can lead dysbiosis potential disruptions interaction, either with epithelial barrier or directly resident microorganisms.

Language: Английский

Citations

0
Dietary zinc restriction mimics protein restriction and extends lifespan in Drosophila DOI Creative Commons
Hina Kosakamoto,

Hide Aikawa,

Souto Kitazawa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Abstract Dietary restriction extends lifespan in model organisms, mainly through dietary amino acids. Compared to macronutrients, the effect of micronutrients on organismal has not been intensively investigated. Here, using a synthetic diet, we test whether each micronutrient, including vitamins and minerals, affects fecundity adult Drosophila . While many these have either negative or no impact lifespan, zinc (Zn) alone can increase it. Zn (ZnR) decreases fecundity, increases starvation resistance, promotes preference for feeding acids, females, phenocopying acid restriction. Our study demonstrates that intake trace elements profound impacts physiology limiting may be strategy improve healthspan animals.

Language: Английский

Citations

2
Role of Rab5 early endosomes in regulating Drosophila gut antibacterial response DOI Creative Commons
Manish Joshi,

Annelise Viallat-Lieutaud,

Julien Royet

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(8), P. 107335 - 107335

Published: July 11, 2023

Interactions between prokaryotes and eukaryotes require a dialogue MAMPs PRRs. In Drosophila, bacterial peptidoglycan is detected by PGRP receptors. While the components of signaling cascades activated upon PGN/PGRP interactions are well characterized, little known about subcellular events that translate these early steps into target gene transcription. Using Drosophila enteric infection model, we show gut-associated bacteria can induce formation intracellular PGRP-LE aggregates which colocalized with endosome marker Rab5. Combining microscopic RNA-seq analysis, demonstrate RNAi inactivation endocytosis pathway in gut affects expression essential regulators NF-κB response leading not only to disruption immune locally but also at systemic level. This work sheds new light on involvement control intestinal infection.

Language: Английский

Citations

5
Vitamin B12 ameliorates gut epithelial injury via modulating the HIF-1 pathway and gut microbiota DOI Creative Commons

Chenxi Feng,

Jinhua Yan, Ting Luo

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Sept. 11, 2024

Language: Английский

Citations

1