mosGILT controls innate immunity and germ cell development in Anopheles gambiae

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 9, 2024

Gene-edited mosquitoes lacking a gamma-interferon-inducible lysosomal thiol reductase-like protein, namely (mosGILT

Language: Английский

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Cereal weevils' antimicrobial peptides: at the crosstalk between development, endosymbiosis and immune response

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

Interactions between animals and microbes are ubiquitous in nature strongly impact animal physiology. These interactions shaped by the host immune system, which responds to infections contributes tailor associations with beneficial microorganisms. In many insects, symbiotic not only include gut commensals, but also intracellular bacteria, or endosymbionts. Endosymbionts housed within specialized cells, bacteriocytes, transmitted vertically across generations. Host–endosymbiont co-evolution shapes endosymbiont genome fights against microbial intruders, ensures preservation of endosymbionts control their load location. The cereal weevil Sitophilus spp. is a remarkable model study evolutionary adaptation system endosymbiosis owing its binary association unique, relatively recently acquired nutritional endosymbiont, Sodalis pierantonius . This Gram-negative bacterium has experienced size shrinkage observed long-term endosymbioses retained immunogenicity. We focus here on sixteen antimicrobial peptides (AMPs) identified oryzae expression patterns different tissues, along development upon challenges, address potential functions defensive response homeostasis insect life cycle. article part theme issue ‘Sculpting microbiome: how factors determine respond colonization’.

Language: Английский

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Untangling the gordian knot: The intertwining interactions between developmental hormone signaling and epigenetic mechanisms in insects

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 585, P. 112178 - 112178

Published: Feb. 9, 2024

Language: Английский

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Insect nuclear receptors: From orphans to ligands

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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ENaC is a host susceptibility factor to bacterial infections in cystic fibrosis context

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 23, 2025

Cystic fibrosis (CF) is a genetic disease caused by dysfunction in the Fibrosis Transmembrane Conductance Regulator (CFTR) chloride channel. Patients with CF are hypersusceptible to Mycobacterium abscessus infection, fast-growing mycobacterium and harmful opportunistic pathogen. Although CFTR known as host susceptibility factor for M. functional impact of trimeric Epithelial sodium Channel (ENaC), whose activity negatively regulated CFTR, towards infection has not been explored yet. To address this issue, we took advantage miR-263a deficient Drosophila presenting CF-like phenotype due ENaC hyperactivity (ENaC+ ). We observed that ENaC+ flies were Cftr-deficient flies. The hypersensitivity was fully rescued blocking hyperactivity, both chemically genetically. Furthermore, per se detrimental Drosophila, they unable mount an efficient humoral immune response. Upon failed upregulate 20-hydroxyecdysone production, which subsequently altered production protective antimicrobial peptides against abscessus. Overall, our results show plays key role and, correlatively other pathogens.

Language: Английский

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Expression of Toll-like receptors in the cerebellum during pathogenesis of prion disease

Frontiers in Behavioral Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: April 18, 2024

Prion diseases, such as scrapie, entail the accumulation of disease-specific prion protein (PrPSc) within brain. Toll-like receptors (TLRs) are crucial components pattern recognition system. They recognize pathogen-associated molecular patterns (PAMPs) and play a central role in orchestrating host innate immune responses. The expression levels nervous system (CNS) were not well-defined. To establish model diseases BALB/C mice, 22L strain was employed. features analyzed, cerebellum exhibited severe pathological changes. TLR1-13 measured using quantitative polymerase chain reaction (qPCR) at time points 60, 90, 120, final end point (145 days post-infection). During pathogenesis, 1, 2, 7, 8, 9 increased time-dependent manner. This trend mirrored PrPSc (the isoform protein) glial fibrillary acidic protein. Notably, point, significantly elevated. Protein level TLR7 TLR9 showed increasing 22L-infected mice. A deeper understanding could shed light on their initiating responses various stages during pathogenesis. insight is particularly relevant when considering TLRs potential therapeutic targets for diseases.

Language: Английский

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Ecdysone promotes gene- and pathogen-specific immune responses to Micrococcus luteus and Bacillus subtilis in Drosophila S2 cells

Journal of Insect Physiology, Journal Year: 2024, Volume and Issue: 159, P. 104710 - 104710

Published: Sept. 15, 2024

Language: Английский

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Pathogen-Mediated Alterations of Insect Chemical Communication: From Pheromones to Behavior

Pathogens, Journal Year: 2023, Volume and Issue: 12(11), P. 1350 - 1350

Published: Nov. 14, 2023

Pathogens can influence the physiology and behavior of both animal plant hosts in a manner that promotes their own transmission dispersal. Recent research focusing on insects has revealed these manipulations extend to production pheromones, which are pivotal chemical communication. This review provides an overview current state available data concerning impacts bacterial, viral, fungal, eukaryotic pathogens communication across different insect orders. While our understanding pathogenic bacteria host profiles is still limited, viral infections have been shown induce behavioral changes host, such as altered pheromone production, olfaction, locomotion. Entomopathogenic fungi affect by manipulating cuticular hydrocarbons while various parasites observed affecting pheromones other cues. The effects induced explored context evolutionary advantages they confer pathogen. molecular mechanisms governing pathogen-mediated changes, well dynamic mutually influential relationships between pathogen its poorly understood. A deeper comprehension will prove invaluable identifying novel targets perspective practical applications aimed at controlling detrimental species.

Language: Английский

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Single-cell transcriptomics reveals subset-specific metabolic profiles underpinning the bronchial epithelial response to flagellin

iScience, Journal Year: 2024, Volume and Issue: 27(9), P. 110662 - 110662

Published: Aug. 3, 2024

Airway epithelial cells represent the first line of defense against respiratory pathogens. Flagellin drives motility many mucosal pathogens and has been suggested as an immune enhancing adjunctive therapeutic in infections airways. This study leveraged single-cell RNA sequencing to determine cell-specific effects flagellin primary human bronchial growing air-liquid interface. Seven cell clusters were identified, including ciliated cells, ionocytes, several states basal secretory which only inflammatory demonstrated a proportional increase response flagellin. Inflammatory showed evidence metabolic reprogramming toward aerobic glycolysis, while transcriptome profiles indicated enhanced oxidative phosphorylation. Inhibition mTOR prevented shift glycolysis reduced gene transcription specifically cells. These data demonstrate functional heterogeneity airway epithelium upon exposure

Language: Английский

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Cereal Weevil’s Antimicrobial Peptides: At the Crosstalk between Development, Endosymbiosis and Immune Response

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 29, 2023

Summary Interactions between animals and microbes are ubiquitous in nature strongly impact animal physiology. These interactions shaped by the host immune system, which responds to infections contributes tailor associations with beneficial microorganisms. In many insects, symbiotic not only include gut commensals, but also intracellular bacteria, or endosymbionts. Endosymbionts housed within specialised cells, bacteriocytes, transmitted vertically across generations. Host-endosymbiont co-evolution shapes endosymbiont genome fights against microbial intruders, ensures preservation of endosymbionts control their load location. The cereal weevil Sitophilus spp. is a remarkable model study evolutionary adaptation system endosymbiosis since its binary association unique, relatively recently acquired nutritional endosymbiont, Sodalis pierantonius . This Gram-negative bacterium has experienced size shrinkage observed long-term endosymbioses retained immunogenicity. We focus here on 16 antimicrobial peptides (AMPs) identified oryzae expression patterns different tissues, along development upon challenges, address potential functions defensive response homeostasis insect life cycle.

Language: Английский

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