Irreproducibility of transgenerational learned pathogen-aversion response in C. elegans DOI Open Access

D Patrick Gainey,

Andrey V. Shubin,

Craig P. Hunter

et al.

Published: Aug. 21, 2024

Here we report our attempt to replicate reports of transgenerational epigenetic inheritance in Caenorhabditis elegans . Published results from multiple laboratories show that C. adults and their F1 embryos exposed the pathogen Pseudomonas aeruginosa aversion behavior a exposure-dependent increase daf-7/TGF β reporter gene expression. However, one group persistence elevated daf-7 expression F2, F3, F4 generations. In contrast, failed consistently detect either avoidance response or beyond generation. The experimental methods are well-described, source materials readily available, including samples reporting laboratory, explored variety environmental conditions likely account for lab-to-lab variability. None these adjustments altered results. Thus, conclude this example lacks robustness required reliable replication investigation.

Language: Английский

Epigenetics and individuality: from concepts to causality across timescales DOI
Amy K. Webster, Patrick C. Phillips

Nature Reviews Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Language: Английский

Citations

4
Pseudomonas aeruginosa modulates both Caenorhabditis elegans attraction and pathogenesis by regulating nitrogen assimilation DOI Creative Commons

Jacob Marogi,

Coleen T. Murphy, Cameron Myhrvold

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 10, 2024

Language: Английский

Citations

8
C. elegans transgenerational avoidance of P. fluorescens is mediated by the Pfs1 sRNA and vab-1 DOI Creative Commons
Renee Seto, R. Lane Brown, Rachel Kaletsky

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(17)

Published: April 23, 2025

In its natural habitat, Caenorhabditis elegans must distinguish friend from foe. Pseudomonas are abundant in the worm’s environment and can be nutritious or pathogenic. Previously, we found that worms learn to avoid aeruginosa vranovensis through a small RNA (sRNA)–mediated pathway targeting C. gene maco-1 , this behavior is inherited for four generations. Here, show learns transgenerationally another pathogenic bacteria fluorescens 15 (PF15). The PF15 sRNA, Pfs1, targets VAB-1 ephrin receptor 16 nt of perfect match, suggesting evolution distinct bacterial sRNA/ target pair. Knockdown both vab-1 induce avoidance, loss reduces expression, placing genes sRNA-targeted avoidance pathway. Thus, multiple act as sRNAs, expanding possibilities trans-kingdom regulation behavior.

Language: Английский

Citations

1
Perception of a pathogenic signature initiates intergenerational protection DOI
Corinne L. Pender, Julian G. Dishart, Holly K. Gildea

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

5
Irreproducibility of transgenerational learned pathogen-aversion response inC. elegans DOI Creative Commons

D Patrick Gainey,

Andrey V. Shubin,

Craig P. Hunter

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 3, 2024

Abstract Here we report our attempt to replicate reports of transgenerational epigenetic inheritance in Caenorhabditis elegans . Published results from multiple laboratories show that C. adults and their F1 embryos exposed the pathogen Pseudomonas aeruginosa aversion behavior a exposure-dependent increase daf-7/TGFβ reporter gene expression. However, one group persistence elevated daf-7 expression F2, F3, F4 generations. In contrast, failed consistently detect either avoidance response or beyond generation. We did confirm dsRNA transport proteins SID-1 SID-2 are required for intergenerational (F1) avoidance, but not Furthermore, reanalysis RNA seq data provides additional evidence this inherited PA14 may be mediated by small RNAs. The experimental methods well-described, source materials readily available, including samples reporting laboratory, explored variety environmental conditions likely account lab-to-lab variability. None these adjustments altered results. conclude example lacks robustness, response, daf-7p::gfp progeny, requires sid-1 sid-2 , identify candidate siRNAs target genes mediate response.

Language: Английский

Citations

4
Improving the odds of survival: transgenerational effects of infections DOI Creative Commons
Victoria M. Spanou, Theano Andriopoulou, Evangelos J. Giamarellos‐Bourboulis

et al.

EMBO Molecular Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Abstract Recent studies argue for a novel concept of the role chromatin as carrier epigenetic memory through cellular and organismal generations, defining coordinating gene activity states physiological functions. Environmental insults, such exposures to unhealthy diets, smoking, toxic compounds, infections, can epigenetically reprogram germ-line cells influence offspring phenotypes. This review focuses on intergenerational transgenerational inheritance in different plants, animal species humans, presenting up-to-date evidence arguments effects light Darwinian Lamarckian evolutionary theories. An overview changes induced by infection or other immune challenges is presented, how these changes, known epimutations, contribute shaping The mechanisms that mediate transmission alterations via germline are also discussed. Understanding relationship between environmental fluctuations, resistance, susceptibility diseases critical unraveling disease etiology adaptive evolution.

Language: Английский

Citations

0
New insights into how parental worms protect their offspring DOI Creative Commons

Chenming Zeng,

Eric A. Miska

Cell Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Language: Английский

Citations

0
Interspecies relationships of natural amoebae and bacteria with C. elegans create environments propitious for multigenerational diapause DOI Creative Commons

Marcela Serey,

Esteban Retamales,

G. Rivas Ibáñez

et al.

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

ABSTRACT The molecular and physical communication within the microscopic world underpins entire web of life as we know it. However, how organisms, such bacteria, amoebae, nematodes—all ubiquitous—interact to sustain their ecological niches, particularly associations generate influence behavior, remains largely unknown. In this study, developed a framework examine long-term interactions between microbes animals. From soil samples collected in temperate, semi-arid climate, isolated culturable bacterial genera, including Comamonas , Stenotrophomonas Chryseobacterium Rhodococcus well amoeba, Tetramitus . This microbial ensemble was fed nematode C. elegans experiments spanning over 20 generations assess developmental rate, dauer entry, fertility, feeding behavior. Our findings reveal that nematodes create stable environment where no species are exhausted, enter diapause after several generations. We have termed phenomenon formation on naturally derived ensembles (DaFNE). DaFNE occurs across range optimal temperatures, from 15°C 25°C, is dependent nematode’s pheromone biosynthesis pathway. intensifies with each passing generation, exhibiting both strong intergenerational transgenerational effects. Moreover, RNA interference (RNAi) pathway—both systemic cell-autonomous—is essential for initiating DaFNE, while heritable RNAi effectors required its These indicate RNA-mediated plays critical role bacterially induced behaviors natural environments. IMPORTANCE Microscopic most abundant multicellular animals Earth, which implies they evolved highly successful relationships associated microbiota. little known about behavior influenced complex ecosystems multiple organisms interact. used four bacteria an amoeba ecosystem explore behavioral responses Caenorhabditis 8 week period. striking finding nematodes’ commitment form hibernation diapause. results suggest nature may frequently result partners. requires production pheromones, pathway, indicating microbiota play role. Interestingly, at higher fewer needed trigger suggesting mild increase temperature promote environments without causing stress

Language: Английский

Citations

0
Reported transgenerational responses to Pseudomonas aeruginosa in Caenorhabditis elegans are not robust DOI Creative Commons
Daniel Patrick Gainey,

Andrey V. Shubin,

Craig P. Hunter

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: March 26, 2025

We report our attempt to replicate reports of transgenerational epigenetic inheritance in Caenorhabditis elegans . Multiple laboratories that C. adults and their F1 embryos exposed the pathogen Pseudomonas aeruginosa show aversion behavior increased daf-7/TGFβ reporter gene expression. However, results from one group persistence both through F4 generation. failed consistently detect either avoidance response or elevated daf-7 expression beyond confirmed dsRNA transport proteins SID-1 SID-2 are required for intergenerational (F1) avoidance, but not Reanalysis RNA seq data provides additional evidence this inherited PA14 may be mediated by small RNAs. The experimental methods well-described, source materials readily available, including samples reporting laboratory, we explored a variety environmental conditions likely account lab-to-lab variability. None these adjustments altered results. conclude example lacks robustness, confirm response, daf-7p::gfp progeny, requires sid-1 sid-2 , identify candidate siRNAs target genes mediate response.

Language: Английский

Citations

0
Molecular Requirements for C. elegans Transgenerational Epigenetic Inheritance of Pathogen Avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

et al.

eLife, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

C. elegans’ major food source is bacteria, and worms are naturally attracted to many bacterial species, including pathogenic Pseudomonas ; in fact, prefer PA14 as well wild bacteria over the lab E. coli strain (OP50) standardly used laboratory setting. Many labs have shown that despite this natural attraction PA14, prior exposure causes instead avoid PA14. This behavioral switch can happen on a relatively fast time scale, even within duration of choice assay. Here we show accurate assessment animals’ true first requires use paralytic (azide) trap at their initial choice, prevent from avoidance assay period. We previously discovered elegans 25°C plate-grown 20°C for 24hrs not only leads these animals switching but also progeny avoiding naïve state, persists through F4 generation. Other types training cause P0 and/or F1 avoidance, do induce transgenerational (F2 beyond) inheritance. showed (P0-F4) learned mediated by P11, small RNA produced P11 both necessary sufficient epigenetic inheritance behavior. highly expressed our standard growth conditions (25°C surfaces), other conditions, suggesting reported failure observe F2-F4 most likely due absence expression experimenters’ conditions. Through mutant analyses, tested genes – germline regulators, uptake, interference/processing, chromatin modifiers, neuronal - involvement pathogen allowing us better understand molecular requirements process. found strains TEI least two P. vranovensis fluorescens 15, avoidance. The induced each species functions specific, distinct (Pv1 Pfs1 15 , respectively) either directly or indirectly reduce levels gene maco-1 which turn regulates daf-7 ASI neuron subsequent conservation multiple components mechanism across suggests behavior be functional physiologically important

Language: Английский

Citations

0