GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex DOI Creative Commons
Chin‐Mei Lee, Man‐Wah Li, Ann Feke

et al.

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Aug. 21, 2019

Abstract ZEITLUPE (ZTL), a photoreceptor with E3 ubiquitin ligase activity, communicates end-of-day light conditions to the plant circadian clock. It still remains unclear how ZTL protein accumulates in but does not destabilize target proteins before dusk. Two deubiquitylating enzymes, UBIQUITIN-SPECIFIC PROTEASE 12 and 13 (UBP12 UBP13), which regulate clock period ubiquitylation manner opposite ZTL, associate complex. Here we demonstrate that interacting partner, GIGANTEA (GI), recruits UBP12 UBP13 We show loss of reduces GI levels through post-transcriptional mechanism. Furthermore, is unable accumulate normal ubp mutants. This demonstrates complex contains both ubiquitin-conjugating -deconjugating these two opposing enzyme types are necessary for pacing. shows enzymes core element clocks, conserved from plants animals.

Language: Английский

Circadian systems biology: When time matters DOI Creative Commons

Luise Fuhr,

Mónica Abreu, J. Patrick Pett

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2015, Volume and Issue: 13, P. 417 - 426

Published: Jan. 1, 2015

The circadian clock is a powerful endogenous timing system, which allows organisms to fine-tune their physiology and behaviour the geophysical time. interplay of distinct set core-clock genes proteins generates oscillations in expression output target temporally regulate numerous molecular cellular processes. study at organismal as well level outlines field chronobiology, has been highly interdisciplinary ever since its origins. development high-throughput approaches enables systems level. In addition experimental approaches, computational models exist allow analysis rhythmic properties network. Such mathematical aid mechanistic understanding can be used predict outcomes perturbations components, thereby generating new hypotheses regarding putative function particular genes. Perturbations system are linked dysfunctions may result severe pathologies including cancer. A comprehensive knowledge crucial develop procedures investigate associated with deregulated clock.In this manuscript we review combination methodologies, bioinformatics theoretical that have essential explore remarkable timing-system. an integrative approach provide strategies regard chronotherapeutic treatment insights concerning restoration clock-associated diseases.

Language: Английский

Citations

76
Online Period Estimation and Determination of Rhythmicity in Circadian Data, Using the BioDare Data Infrastructure DOI

Anne Moore,

T. Zieliński, Andrew J. Millar

et al.

Methods in molecular biology, Journal Year: 2014, Volume and Issue: unknown, P. 13 - 44

Published: Jan. 1, 2014

Language: Английский

Citations

72
Phosphorylation of Phosphoenolpyruvate Carboxylase Is Essential for Maximal and Sustained Dark CO2 Fixation and Core Circadian Clock Operation in the Obligate Crassulacean Acid Metabolism Species Kalanchoë fedtschenkoi DOI Creative Commons
Susanna Flavia Boxall, Louisa V. Dever, Jana Kneřová

et al.

The Plant Cell, Journal Year: 2017, Volume and Issue: 29(10), P. 2519 - 2536

Published: Sept. 8, 2017

Phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31) catalyzes primary nocturnal CO2 fixation in Crassulacean acid metabolism (CAM) species. CAM PPC is regulated posttranslationally by a circadian clock-controlled protein kinase called phosphoenolpyruvate (PPCK). PPCK phosphorylates during the dark period, reducing its sensitivity to feedback inhibition malate and thus enhancing stored malate. Here, we report generation characterization of transgenic RNAi lines obligate species Kalanchoë fedtschenkoi with reduced levels KfPPCK1 transcripts. Plants or no detectable phosphorylation displayed up 66% reduction total period fixation. These perturbations paralleled accumulation at dawn decreased starch turnover. Loss oscillations transcript abundance was accompanied loss many core clock genes, suggesting that perturbing only known link between feeds back perturb central itself. This work shows control prolongs activity throughout K. fedtschenkoi, optimizing CAM-associated fixation, accumulation, productivity, robustness.

Language: Английский

Citations

69
Multi-level Modulation of Light Signaling by GIGANTEA Regulates Both the Output and Pace of the Circadian Clock DOI Creative Commons
María A. Nohales, Wanlu Liu, Tomás Duffy

et al.

Developmental Cell, Journal Year: 2019, Volume and Issue: 49(6), P. 840 - 851.e8

Published: May 16, 2019

Language: Английский

Citations

69
GIGANTEA recruits the UBP12 and UBP13 deubiquitylases to regulate accumulation of the ZTL photoreceptor complex DOI Creative Commons
Chin‐Mei Lee, Man‐Wah Li, Ann Feke

et al.

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Aug. 21, 2019

Abstract ZEITLUPE (ZTL), a photoreceptor with E3 ubiquitin ligase activity, communicates end-of-day light conditions to the plant circadian clock. It still remains unclear how ZTL protein accumulates in but does not destabilize target proteins before dusk. Two deubiquitylating enzymes, UBIQUITIN-SPECIFIC PROTEASE 12 and 13 (UBP12 UBP13), which regulate clock period ubiquitylation manner opposite ZTL, associate complex. Here we demonstrate that interacting partner, GIGANTEA (GI), recruits UBP12 UBP13 We show loss of reduces GI levels through post-transcriptional mechanism. Furthermore, is unable accumulate normal ubp mutants. This demonstrates complex contains both ubiquitin-conjugating -deconjugating these two opposing enzyme types are necessary for pacing. shows enzymes core element clocks, conserved from plants animals.

Language: Английский

Citations

67