Journal of Asia-Pacific Entomology, Journal Year: 2018, Volume and Issue: 22(1), P. 56 - 62
Published: Dec. 3, 2018
Language: Английский
Pest Management Science, Journal Year: 2019, Volume and Issue: 76(1), P. 26 - 41
Published: Aug. 16, 2019
Abstract RNA interference is a phenomenon in which the introduction of double‐stranded (dsRNA) into cells triggers degradation complementary messenger sequence‐specific manner. Suppressing expression vital genes could lead to insect death, therefore this technology has been considered as potential strategy for pest control. There are three main routes dsRNA administration insects: (i) injections hemolymph, (ii) topical, and (iii) feeding. In review, we focus on through We summarize novel strategies that have developed improve efficacy method, such use nano‐based formulations, engineered microorganisms, transgenic plants. also expose hurdles be overcome order technique reliable management method. © 2019 Society Chemical Industry
Language: Английский
Citations
149
Frontiers in Plant Science, Journal Year: 2016, Volume and Issue: 7
Published: Sept. 22, 2016
The leucine rich repeat receptor like kinases (LRRK) constitute the largest subfamily of (RLK), which play critical roles in plant development and stress responses. Herein, we identified 531
Language: Английский
Citations
73
Frontiers in Physiology, Journal Year: 2019, Volume and Issue: 10
Published: May 8, 2019
The whitefly B. tabaci is a global pest and transmits extremely important plant viruses especially begomoviruses, that cause substantial crop losses. one of the top invasive species worldwide have developed resistance to all major pesticide classes. One promising alternative ways for controlling this studying its genetic makeup identifying specific target proteins which are critical development ability transmit viruses. Tomato yellow leaf curl virus (TYLCV) most economically well-studied begomovirus transmitted by tabaci, in persistent-circulative manner. Recently, we reported Cyclophilin B (CypB) heat shock protein 70 (hsp70) interact co-localize with TYLCV midgut, on transmission pathway, both significant role transmission. Here, extended previous work used Tobacco rattle (TRV) plant-mediated RNA silencing system knocking down genes testing effect their viability Portions these two were cloned into TRV constructs tomato plants infected feeding experiments. Following TRV-plants, expression levels cypB hsp70 adult significantly decreased over 72 h period. knockdown was further shown first generation silenced whiteflies, where phenotypic abnormalities adult, wing, nymph bacteriosomes structure observed. Additionally, high mortality rates reached more than 80% among nymphs adults obtained. Finally, showed under lab conditions. Our results suggest profound effects TYLCV.
Language: Английский
Citations
64
Insect Molecular Biology, Journal Year: 2024, Volume and Issue: 33(5), P. 467 - 480
Published: Feb. 9, 2024
The function of DNA methylation in insects and the methyltransferase (Dnmt) genes that influence remains uncertain. We used RNA interference to reduce gene expression Dnmt1 within whitefly Bemisia tabaci (Hemiptera:Aleyrodidae; Gennadius), a hemipteran species relies on for proper gametogenesis. then RNA-seq test an priori hypothesis meiosis-related genetic pathways would be perturbed. generally did not find overall effect pathways. However, we found Wnt pathway, associated with entry into meiosis vertebrates, were differentially expressed. Our results are consistent knockdown influencing specific causing general transcriptional response. This is finding also seen other insect species. characterised methylome B. assessed cytosine methylation. has characteristics comparable hemipterans regarding level, enrichment bodies, bimodal distribution methylated/non-methylated genes. Very little differential was observed, difference differences expression. presents interesting new candidate pathway future studies.
Language: Английский
Citations
6
Scientific Reports, Journal Year: 2017, Volume and Issue: 7(1)
Published: Sept. 27, 2017
RNA interference (RNAi) in transgenic maize has recently emerged as an alternative mode of action for western corn rootworm (Diabrotica virgifera virgifera) control which can be combined with protein-based options improved root protection and resistance management. Currently, RNAi-based focused on suppression genes that when silenced lead to larval mortality. We investigated reproduction through RNAi by targeting two reproductive genes, dvvgr dvbol, the goal reducing insect fecundity a new tool pest The results demonstrated exposure adult beetles, well larvae or dvbol dsRNA artificial diet, caused reduction fecundity. Furthermore, beetles from feeding roots expressing also showed significant reduction. This is first report fitness plant-mediated RNAi, demonstrating feasibility management rootworm.
Language: Английский
Citations
58
Frontiers in Plant Science, Journal Year: 2016, Volume and Issue: 7
Published: Nov. 28, 2016
The Ca2+/cation antiporters (CaCA) superfamily proteins play vital function in Ca2+ ion homeostasis, which is an important event during development and defense response. Molecular characterization of these has been performed certain plants, but they are still not characterized Triticum aestivum (bread wheat). Herein, we identified thirty four TaCaCA proteins, were classified into TaCAX, TaCCX, TaNCL TaMHX protein families based on their structural organization evolutionary relation with earlier reported proteins. Since the T. comprises allohexaploid genome, genes derived from each A, B D subgenome homeologous chromosome (HC), except chromosome-group 1. Majority more than one HCs family that considered as (HGs) due to high similarity other. These HGs showed comparable gene structures terms exon/intron domain architecture. comprised two Na_Ca_ex domains. However, TaNCLs consisted additional EF-hand calcium binding motifs. Each about ten transmembrane α-repeat regions specifically conserved signature motifs TaNCL, had single α-repeat. Variable expression most various developmental stages suggested specified role development. constitutively a few like TaCAX1-A TaNCL1-B indicated throughout plant growth modulated biotic (fungal infections) abiotic stresses (heat, drought, salt) stress TaCCX found highly affected stresses. However individual needs be established. present study unfolded opportunity for detail functional utilization future crop improvement programs.
Language: Английский
Citations
56
Insect Molecular Biology, Journal Year: 2017, Volume and Issue: 27(2), P. 221 - 233
Published: Dec. 11, 2017
Abstract Vitellogenin (Vg) and its receptor (VgR) play a key role in the reproductive process development of insects. Aphids are group high‐fecundity insect species with pseudoplacental viviparity, but roles their Vg VgR genes have not been investigated yet. The brown citrus aphid, Aphis ( Toxoptera ) citricidus , is major pest main vector Citrus tristeza closterovirus . In this study, we identified characterized these two genes, designated as AcVg AcVgR from aphid. We found that has lost DUF1943 domain present other Vgs. Silencing led to delay nymph–adult transition, prolonged prereproductive period, shortened which turn resulted slower embryonic fewer new‐born nymphs. Interestingly, silencing decreased transcript level increased levels addition, / had similar phenotypes between alate apterous morphs, suggesting functions same wing morphs Our results demonstrate involved various aspects aphid reproduction. Further studies on synthesis could help elucidate mechanism provide information will be useful for developing new control strategies.
Language: Английский
Citations
54
Pesticide Biochemistry and Physiology, Journal Year: 2021, Volume and Issue: 180, P. 104981 - 104981
Published: Nov. 1, 2021
Language: Английский
Citations
36
Frontiers in Physiology, Journal Year: 2018, Volume and Issue: 9
Published: Sept. 11, 2018
Conopomorpha sinensis Bradley is the dominant borer pest of Litchi chinesis and Euphoria longan. Current management C. relies upon insecticide application to adult moths. In addition direct mortality induced by insecticides, a sublethal dose insecticides also affects growth, survival reproduction in exposed insects. Vitellogenin (Vg) vitellogenin receptor (VgR) are normally identified as essential reproduction-related proteins this study, we characterized these two genes from sinensis, investigated their differential responses concentrations insecticide. Cloned CsVg CsVgR consist 5391 5424-bp open reading frames, which encode 1796 1807 amino acid residues, respectively. The protein contains typical vitellogenin, DUF1943 VWFD domains other reported lepidopteran Vgs. was low density lipoprotein with 2 highly conserved LBD EGF precursor domains, one hydrophobic transmembrane domain, cytoplasmic 13 putative N-glycosylation sites. We next assessed effect four major on egg-laying sinensis. toxicity against varied among tested, LC50 values ranging 0.23 ppm for chlorpyrifos 20.00 β-cypermethrin, emamectin benzoate (EB) showed significant negative impact egg-laying, rate, ovarian development, mating rate at LC30 doses. Further investigation that transcriptional level were impaired different way 24 h, 48 h 72 after EB exposure, result agreement diminished concentration EB-treated group. A repressed transcription observed treatment, suggesting elicits delayed response abundance CsVgR. These results established roles insecticides. might be better parameter than assessing
Language: Английский
Citations
43
Archives of Insect Biochemistry and Physiology, Journal Year: 2018, Volume and Issue: 100(2)
Published: Nov. 28, 2018
Abstract Whiteflies cause considerable losses to crops, directly by feeding, and indirectly transmission of viruses. The current control methods consist a combination different tactics, mainly still relying on unsafe non‐ecofriendly chemical control. RNA interference (RNAi) is post‐transcriptional gene‐silencing strategy in which double‐stranded (dsRNA), corresponding specifically target gene, introduced organism. Research RNAi the previous decade has shown its success as potential insect strategy, can be highly species‐specific environment friendly. In whiteflies, dsRNA delivery through oral route opened possibilities for management plant‐mediated RNAi. To date, several genes have been targeted whiteflies these assays demonstrated manage at lab level. However, further research investments are needed move toward an application field this review, first time, we collected literature silencing via discuss whitefly pest We also likely methods, including transgenic planta symbiont‐mediated delivery, studying interfering with insecticide resistance mechanisms virus whiteflies.
Language: Английский
Citations
42