Genome-wide characterization and expression analysis suggested diverse functions of the mechanosensitive channel of small conductance-like (MSL) genes in cereal crops DOI Creative Commons
Amandeep Kaur, Mehak Taneja, Shivi Tyagi

et al.

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: Oct. 6, 2020

Abstract Mechanosensitive ion channels are pore-forming transmembrane proteins that allow ions to move down their electrochemical gradient in response mechanical stimuli. They participate many plant developmental processes including the maintenance of plastid shape, pollen tube growth, etc. Herein, a total 11, 10, 6, 30, 9, and 8 MSL genes were identified Aegilops tauschii , Hordeum vulgare, Sorghum bicolor, Triticum aestivum urartu, Zea mays, respectively . These located on various chromosomes respective cereal, while s T. urartu found scaffolds. The phylogenetic analysis, subcellular localization, sequence homology suggested clustering MSLs into two classes. consisted cis -regulatory elements related growth development, responsive light, hormone, stress. Differential expression tissue stages stress conditions revealed precise role development responses. Altered during CaCl 2 Ca 2+ homeostasis signaling. co-expression analysis interactions with other involved defense responses A comparative profiling paralogous either retention function or pseudo-functionalization. present study unfolded characteristics cereals, which will facilitate in-depth functional characterization future studies.

Language: Английский

Genome-Wide Association Study Reveals Novel Genomic Regions for Grain Yield and Yield-Related Traits in Drought-Stressed Synthetic Hexaploid Wheat DOI Open Access
Madhav Bhatta, Alexey Morgounov, Vikas Belamkar

et al.

International Journal of Molecular Sciences, Journal Year: 2018, Volume and Issue: 19(10), P. 3011 - 3011

Published: Oct. 2, 2018

Synthetic hexaploid wheat (SHW; 2n = 6x 42, AABBDD, Triticum aestivum L.) is produced from an interspecific cross between durum (2n 4x 28, AABB, T. turgidum and goat grass 2x 14, DD, Aegilops tauschii Coss.) reported to have significant novel alleles-controlling biotic abiotic stresses resistance. A genome-wide association study (GWAS) was conducted unravel these loci [marker–trait associations (MTAs)] using 35,648 genotyping-by-sequencing-derived single nucleotide polymorphisms in 123 SHWs. We identified 90 MTAs (45, 11, 34 on the A, B, D genomes, respectively) haplotype blocks associated with grain yield yield-related traits including root under drought stress. The phenotypic variance explained by ranged 1.1% 32.3%. Most of (120 out 194) were found genes, 45 genes annotated as having a potential role This result provides further evidence for reliability identified. large number (53) especially D-genome demonstrate SHWs elucidating genetic architecture complex provide opportunity improvement rapidly changing climatic conditions.

Language: Английский

Citations

127
Survey of High Throughput RNA-Seq Data Reveals Potential Roles for lncRNAs during Development and Stress Response in Bread Wheat DOI Creative Commons

Shumayla,

Shailesh Sharma, Mehak Taneja

et al.

Frontiers in Plant Science, Journal Year: 2017, Volume and Issue: 8

Published: June 9, 2017

Long non-coding RNAs (lncRNAs) are a family of regulatory that play essential role in the various developmental processes and stress responses. Recent advances sequencing technology computational methods enabled identification characterization lncRNAs certain plant species, but they less known Triticum aestivum (bread wheat). Herein, we analyzed 52 RNA seq data (>30 billion reads) identified 44,698 T. genome, which were characterized comparison to coding sequences (mRNAs). Similar mRNAs, also derived from each sub-genome chromosome, showed tissue stage specific differential expression, as well. The modulated expression during abiotic stresses like heat, drought, salt indicated their putative response. co-expression with vital mRNAs including transcription factors enzymes involved Abscisic acid (ABA) biosynthesis, gene ontology mapping inferred roles numerous biological processes. A few predicted precursor (19 lncRNAs), while some target mimics (1,047 lncRNAs) miRNAs functions. results suggested functions aestivum, unfolded opportunities for functional individual lncRNA future studies.

Language: Английский

Citations

109
Molecular characterization revealed the role of catalases under abiotic and arsenic stress in bread wheat (Triticum aestivum L.) DOI
Shivi Tyagi,

Shumayla,

Madhu Madhu

et al.

Journal of Hazardous Materials, Journal Year: 2020, Volume and Issue: 403, P. 123585 - 123585

Published: Aug. 2, 2020

Language: Английский

Citations

72
Receptor-like Kinases (LRR-RLKs) in Response of Plants to Biotic and Abiotic Stresses DOI Creative Commons
Aigerim Soltabayeva,

Nurbanu Dauletova,

Symbat Serik

et al.

Plants, Journal Year: 2022, Volume and Issue: 11(19), P. 2660 - 2660

Published: Oct. 10, 2022

Plants live under different biotic and abiotic stress conditions, and, to cope with the adversity severity, plants have well-developed resistance mechanisms. The mechanism starts perception of stimuli followed by molecular, biochemical, physiological adaptive measures. family LRR-RLKs (leucine-rich repeat receptor-like kinases) is one such group that perceives also plays important roles in biological processes development. This has been mostly studied model plant, Arabidopsis thaliana, some extent other plants, as Solanum lycopersicum, Nicotiana benthamiana, Brassica napus, Oryza sativa, Triticum aestivum, Hordeum vulgare, Brachypodium distachyon, Medicago truncatula, Gossypium barbadense, Phaseolus vulgaris, tuberosum, Malus robusta. Most tend form combinations LRR-RLKs-complexes (dimer, trimer, tetramers), them were observed receptors immune responses, cell death, plant development processes. However, less known about function(s) response stresses. Here, we give recent updates LRR-RLK receptors, specifically focusing on their involvement stresses A. thaliana. Furthermore, studies are homologous reviewed relation role triggering against and/or exploring additional function(s). present interactions among confirmed through experiments. Moreover, based GENEINVESTIGATOR microarray database analysis, predict potential genes involved certain whose function may be explored.

Language: Английский

Citations

61
An update on evolutionary, structural, and functional studies of receptor-like kinases in plants DOI Creative Commons
Jing Liu, Wenjuan Li, Guang Wu

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 31, 2024

All living organisms must develop mechanisms to cope with and adapt new environments. The transition of plants from aquatic terrestrial environment provided opportunities for them exploit additional resources but made vulnerable harsh ever-changing conditions. As such, the transmembrane receptor-like kinases (RLKs) have been extensively duplicated expanded in land plants, increasing number RLKs advanced angiosperms, thus becoming one largest protein families eukaryotes. basic structure consists a variable extracellular domain (ECD), (TM), conserved kinase (KD). Their ECDs can perceive various kinds ligands that activate KD through series auto- trans-phosphorylation events, allowing KDs keep activities as molecular switch stabilizes their intracellular signaling cascades, possibly maintaining cellular homeostasis advantages different environmental RLK may require coreceptor other interactors, which ultimately leads control functions growth development, fertilization, immunity. Therefore, identification might offer unique insight into regulatory mechanism plant development adaptations. Here, we give an overview update recent advances mechanisms.

Language: Английский

Citations

15
Genomic dissection and transcriptional profiling of Cysteine-rich receptor-like kinases in five cereals and functional characterization of TaCRK68-A DOI

Shumayla,

Shivi Tyagi, Alok Sharma

et al.

International Journal of Biological Macromolecules, Journal Year: 2019, Volume and Issue: 134, P. 316 - 329

Published: May 9, 2019

Language: Английский

Citations

59
Thaumatin-like protein kinases: Molecular characterization and transcriptional profiling in five cereal crops DOI
Alok Sharma,

Shumayla,

Shivi Tyagi

et al.

Plant Science, Journal Year: 2019, Volume and Issue: 290, P. 110317 - 110317

Published: Nov. 2, 2019

Language: Английский

Citations

56
Genome-wide association study of resistance to stripe rust (Puccinia striiformis f. sp. tritici) in Sichuan wheat DOI Creative Commons
Xueling Ye, Jian Li,

Yukun Cheng

et al.

BMC Plant Biology, Journal Year: 2019, Volume and Issue: 19(1)

Published: April 16, 2019

Stripe rust (also called yellow rust) is a common and serious fungal disease of wheat (Triticum aestivum L.) caused by Puccinia striiformis f. sp. tritici. The narrow genetic basis modern cultivars rapid evolution the pathogen have been responsible for periodic devastating epidemics diseases. In this study, we conducted genome-wide association study with 44,059 single nucleotide polymorphism markers to identify loci associated resistance stripe in 244 Sichuan accessions, including 79 landraces 165 cultivars, six environments.In all field assessments, 24 accessions displayed stable high rust. Significant correlations among environments were observed both infection (IT) severity (DS), heritability levels found IT DS. Using mixed linear models, 12 quantitative trait (QTLs) significantly and/or DS identified. Two QTLs mapped on chromosomes 5AS 5AL distant from previously identified genes or QTL regions, indicating that they may be novel loci.Our results revealed alleles accumulated germplasm, implying direct indirect selection improved elite breeding programs. favorable could important chromosome regions controlled These can used molecular marker-assisted will useful ongoing effort develop new strong

Language: Английский

Citations

55
Genome-wide identification of the LRR-RLK gene family in peanut and functional characterization of AhLRR-RLK265 in salt and drought stresses DOI
Qi Wang, Xiaobo Zhao, Quanxi Sun

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127829 - 127829

Published: Nov. 4, 2023

Language: Английский

Citations

19
Ca2+/Cation Antiporters (CaCA): Identification, Characterization and Expression Profiling in Bread Wheat (Triticum aestivum L.) DOI Creative Commons
Mehak Taneja, Shivi Tyagi, Shailesh Sharma

et al.

Frontiers in Plant Science, Journal Year: 2016, Volume and Issue: 7

Published: Nov. 28, 2016

The Ca2+/cation antiporters (CaCA) superfamily proteins play vital function in Ca2+ ion homeostasis, which is an important event during development and defense response. Molecular characterization of these has been performed certain plants, but they are still not characterized Triticum aestivum (bread wheat). Herein, we identified thirty four TaCaCA proteins, were classified into TaCAX, TaCCX, TaNCL TaMHX protein families based on their structural organization evolutionary relation with earlier reported proteins. Since the T. comprises allohexaploid genome, genes derived from each A, B D subgenome homeologous chromosome (HC), except chromosome-group 1. Majority more than one HCs family that considered as (HGs) due to high similarity other. These HGs showed comparable gene structures terms exon/intron domain architecture. comprised two Na_Ca_ex domains. However, TaNCLs consisted additional EF-hand calcium binding motifs. Each about ten transmembrane α-repeat regions specifically conserved signature motifs TaNCL, had single α-repeat. Variable expression most various developmental stages suggested specified role development. constitutively a few like TaCAX1-A TaNCL1-B indicated throughout plant growth modulated biotic (fungal infections) abiotic stresses (heat, drought, salt) stress TaCCX found highly affected stresses. However individual needs be established. present study unfolded opportunity for detail functional utilization future crop improvement programs.

Language: Английский

Citations

56