Evolution of neuronal cell classes and types in the vertebrate retina DOI Creative Commons
Joshua Hahn, Aboozar Monavarfeshani,

Mu Qiao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 8, 2023

The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs (Baden et al., 2020). One might expect that retinal cell types evolved to accommodate these varied needs, but this has not been systematically studied. Here, we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a teleost fish, bird, reptile lamprey. Molecular conservation six classes (photoreceptors, horizontal cells, bipolar amacrine ganglion cells [RGCs] Muller glia) striking, with differences correlated evolutionary distance. Major subclasses are also conserved, whereas variation among within or more pronounced. However, an integrative analysis revealed numerous shared based on gene expression programs likely trace back common ancestor jawed vertebrates. degree increases outer (photoreceptors) inner (RGCs), suggesting evolution acts preferentially shape output. Finally, identified mammalian orthologs midget RGCs, which comprise >80% RGCs human retina, subserve high-acuity vision, were believed be primate-specific (Berson, 2008); contrast, mouse <2% RGCs. Projections both primate orthologous overrepresented thalamus, supplies primary cortex. We suggest innovations, descendants evolutionarily ancient decreased size increased number as primates evolved, thereby facilitating high acuity cortical processing information.

Language: Английский

Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes DOI Creative Commons
Nicholas M. Tran, Karthik Shekhar,

Irene E. Whitney

et al.

Neuron, Journal Year: 2019, Volume and Issue: 104(6), P. 1039 - 1055.e12

Published: Nov. 26, 2019

Language: Английский

Citations

535
Understanding the retinal basis of vision across species DOI
Tom Baden, Thomas Euler, Philipp Berens

et al.

Nature reviews. Neuroscience, Journal Year: 2019, Volume and Issue: 21(1), P. 5 - 20

Published: Nov. 28, 2019

Language: Английский

Citations

282
Digital Museum of Retinal Ganglion Cells with Dense Anatomy and Physiology DOI Creative Commons
J. Alexander Bae, Shang Mu, Jinseop S. Kim

et al.

Cell, Journal Year: 2018, Volume and Issue: 173(5), P. 1293 - 1306.e19

Published: May 1, 2018

Language: Английский

Citations

250
Neurotoxic Reactive Astrocytes Drive Neuronal Death after Retinal Injury DOI Creative Commons
Kevin A. Guttenplan, Benjamin K. Stafford,

Rana N. El‐Danaf

et al.

Cell Reports, Journal Year: 2020, Volume and Issue: 31(12), P. 107776 - 107776

Published: June 1, 2020

Glaucoma is a neurodegenerative disease that features the death of retinal ganglion cells (RGCs) in retina, often as result prolonged increases intraocular pressure. We show preventing formation neuroinflammatory reactive astrocytes prevents RGCs normally seen mouse model glaucoma. Furthermore, we these spared are electrophysiologically functional and thus still have potential value for function regeneration retina. Finally, demonstrate depends on combination both an injury to neurons presence astrocytes, suggesting may explain why toxic only some circumstances. Altogether, findings highlight drivers RGC chronic eye.

Language: Английский

Citations

190
Unified classification of mouse retinal ganglion cells using function, morphology, and gene expression DOI Creative Commons

Jillian J. Goetz,

Zachary F. Jessen,

Anne Jacobi

et al.

Cell Reports, Journal Year: 2022, Volume and Issue: 40(2), P. 111040 - 111040

Published: July 1, 2022

Classification and characterization of neuronal types are critical for understanding their function dysfunction. Neuronal classification schemes typically rely on measurements electrophysiological, morphological, molecular features, but aligning such datasets has been challenging. Here, we present a unified mouse retinal ganglion cells (RGCs), the sole output neurons. We use visually evoked responses to classify 1,859 RGCs into 42 types. also obtain morphological or transcriptomic data from subsets these align functional publicly available datasets. create an online database that allows users browse download light using machine learning algorithm. This work provides resource studies RGCs, upstream circuits in retina, projections brain, establishes framework future efforts open distribution.

Language: Английский

Citations

170
Evolution of neuronal cell classes and types in the vertebrate retina DOI Creative Commons
Joshua Hahn, Aboozar Monavarfeshani,

Mu Qiao

et al.

Nature, Journal Year: 2023, Volume and Issue: 624(7991), P. 415 - 424

Published: Dec. 13, 2023

Abstract The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs 1 . Retinal cell types may have evolved to accommodate these varied needs, but this has not been systematically studied. Here we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a bird, reptile, teleost fish lamprey. We found high molecular conservation six retinal classes (photoreceptors, horizontal cells, bipolar amacrine ganglion cells (RGCs) Müller glia), with variation related evolutionary distance. Major subclasses were also conserved, whereas among within or was more pronounced. However, an integrative analysis revealed that numerous are shared species, based on gene expression programmes likely trace back early ancestral vertebrate. degree increased outer (photoreceptors) inner (RGCs), suggesting evolution acts preferentially shape output. Finally, identified rodent orthologues midget RGCs, which comprise than 80% RGCs human retina, subserve high-acuity vision, previously believed be restricted primates 2 By contrast, mouse large receptive fields around 2% RGCs. Projections both primate orthologous overrepresented thalamus, supplies primary cortex. suggest innovations, descendants evolutionarily ancient decreased size number as evolved, thereby facilitating acuity cortical processing information.

Language: Английский

Citations

90
Retinal Ganglion Cell Diversity and Subtype Specification from Human Pluripotent Stem Cells DOI Creative Commons

Kirstin B. Langer,

Sarah K. Ohlemacher,

M. Joseph Phillips

et al.

Stem Cell Reports, Journal Year: 2018, Volume and Issue: 10(4), P. 1282 - 1293

Published: March 22, 2018

Highlights•Unique transcriptional profiles demonstrate diversity among hPSC-derived RGCs•Numerous RGC subtypes characterized from RGCs•Molecular markers identified for through single-cell RNA-seq analysisSummaryRetinal ganglion cells (RGCs) are the projection neurons of retina and transmit visual information to postsynaptic targets in brain. While this function is shared nearly all RGCs, class cell remarkably diverse, comprised multiple subtypes. Previous efforts have numerous animal models, but less attention has been paid human RGCs. Thus, study examined RGCs differentiated pluripotent stem (hPSCs) defined expression subtype-specific markers. Further investigation these was achieved using transcriptomics, confirming combinatorial molecular associated with subtypes, also provided insight into more results describe derivation hPSCs will support future exploration phenotypic functional within

Language: Английский

Citations

108
The sifting of visual information in the superior colliculus DOI Creative Commons
Kyu Hyun Lee,

Alvita Tran,

Zeynep Turan

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: April 14, 2020

Much of the early visual system is devoted to sifting scene for few bits behaviorally relevant information. In cortex mammals, a hierarchical brain areas leads eventually selective encoding important features, like faces and objects. Here, we report that similar process occurs in other major pathway, superior colliculus. We investigate response properties collicular neurons awake mouse with large-scale electrophysiology. Compared superficial layers, neuronal responses deeper layers become more stimuli; invariant location stimuli field; suppressed by repeated occurrence stimulus same location. The memory familiar persists complete absence cortex. Models these neural computations lead specific predictions circuitry

Language: Английский

Citations

107
Calcium-Binding Proteins as Determinants of Central Nervous System Neuronal Vulnerability to Disease DOI Open Access
Richard Fairless, Sarah K. Williams,

Ricarda Diem

et al.

International Journal of Molecular Sciences, Journal Year: 2019, Volume and Issue: 20(9), P. 2146 - 2146

Published: April 30, 2019

Neuronal subpopulations display differential vulnerabilities to disease, but the factors that determine their susceptibility are poorly understood. Toxic increases in intracellular calcium a key factor several neurodegenerative processes, with calcium-binding proteins providing an important first line of defense through ability buffer incoming calcium, allowing neuron quickly achieve homeostasis. Since neurons expressing different have been reported be differentially susceptible degeneration, it can hypothesized rather than just serving as markers neuronal subpopulations, they might actually determinant survival. In this review, we will summarize some evidence expression EF-hand proteins, calbindin, calretinin and parvalbumin, may influence distinct disease processes.

Language: Английский

Citations

91
Mouse dLGN Receives Functional Input from a Diverse Population of Retinal Ganglion Cells with Limited Convergence DOI Creative Commons

Miroslav Román Rosón,

Yannik Bauer, Ann H. Kotkat

et al.

Neuron, Journal Year: 2019, Volume and Issue: 102(2), P. 462 - 476.e8

Published: Feb. 21, 2019

Language: Английский

Citations

78