Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain DOI Creative Commons
Gabriella R Sterne, Hideo Otsuna, Barry J. Dickson

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Sept. 2, 2021

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we generate collection split-GAL4 lines provides precise genetic targeting 138 different cell types adult Drosophila melanogaster, comprising approximately one third all We characterize single-cell anatomy these neurons find they cluster morphology into six supergroups organize discrete anatomical domains. majority local interneurons are not classically polarized, suggesting rich processing, whereas projection tend be conveying information number higher regions. This insight organization generates resources will facilitate further their contributions sensory processing behavior.

Language: Английский

Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Jan. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

3
A split-GAL4 driver line resource for Drosophila neuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Language: Английский

Citations

3
Connectome-driven neural inventory of a complete visual system DOI Creative Commons
Aljoscha Nern, Frank Loesche, Shin-ya Takemura

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form movement across the visual scene. Computing these parallel spatial requires a large network of neurons. Consequently, from flies to humans, regions in brain constitute half its volume. These often have marked structure–function relationships, neurons organized along maps shapes that directly relate roles processing. More than century anatomical studies catalogued detail cell types fly systems 1–3 , behavioural physiological experiments examined capabilities flies. To unravel diversity complex system, careful mapping neural architecture matched tools for targeted exploration this circuitry is essential. Here we present connectome right optic lobe male Drosophila melanogaster acquired using focused ion beam milling scanning electron microscopy. We established comprehensive inventory developed computational framework quantify anatomy. Together, data establish basis interpreting how vision. By integrating analysis connectivity information, neurotransmitter identity expert curation, classified approximately 53,000 into 732 types. are systematically described newly named. Finally, share an extensive collection split-GAL4 lines our neuron-type catalogue. Overall, set unlocks new possibilities systematic investigations vision foundation deeper understanding sensory

Language: Английский

Citations

3
A Systematic Nomenclature for the Drosophila Ventral Nerve Cord DOI Creative Commons
Robert Court, Shigehiro Namiki, J. Douglas Armstrong

et al.

Neuron, Journal Year: 2020, Volume and Issue: 107(6), P. 1071 - 1079.e2

Published: Sept. 1, 2020

Drosophila melanogaster is an established model for neuroscience research with relevance in biology and medicine. Until recently, on the brain was hindered by lack of a complete uniform nomenclature. Recognizing this, Ito et al., 2014Ito K. Shinomiya M. Armstrong D.J. Boyan G. Hartenstein V. Harzsch S. Heisenberg Homberg U. Jenett A. al.A Systematic Nomenclature Insect Brain.Neuron. 2014; 81: 755-765Abstract Full Text PDF PubMed Scopus (329) Google Scholar produced authoritative nomenclature adult insect brain, using as reference. Here, we extend this to thoracic abdominal neuromeres, ventral nerve cord (VNC), provide anatomical description major component nervous system. The VNC locus reception integration sensory information involved generating most locomotor actions that underlie fly behaviors. aim create nomenclature, definitions, spatial boundaries are consistent other insects. work establishes framework provides powerful tool analyzing functional organization VNC.

Language: Английский

Citations

85
Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain DOI Creative Commons
Gabriella R Sterne, Hideo Otsuna, Barry J. Dickson

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Sept. 2, 2021

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we generate collection split-GAL4 lines provides precise genetic targeting 138 different cell types adult Drosophila melanogaster, comprising approximately one third all We characterize single-cell anatomy these neurons find they cluster morphology into six supergroups organize discrete anatomical domains. majority local interneurons are not classically polarized, suggesting rich processing, whereas projection tend be conveying information number higher regions. This insight organization generates resources will facilitate further their contributions sensory processing behavior.

Language: Английский

Citations

84