The plasma metabolome of long COVID patients two years after infection

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Aug. 1, 2023

Abstract One of the major challenges currently faced by global health systems is prolonged COVID-19 syndrome (also known as “long COVID”) which has emerged a consequence SARS-CoV-2 epidemic. It estimated that at least 30% patients who have had will develop long COVID. In this study, our goal was to assess plasma metabolome in total 100 samples collected from healthy controls, patients, and COVID recruited Mexico between 2020 2022. A targeted metabolomics approach using combination LC–MS/MS FIA MS/MS performed quantify 108 metabolites. IL-17 leptin were measured immunoenzymatic assay. The comparison paired COVID-19/long revealed 53 metabolites statistically different. Compared 27 remained dysregulated even after two years. Post-COVID-19 displayed heterogeneous metabolic profile. Lactic acid, lactate/pyruvate ratio, ornithine/citrulline arginine identified most relevant for distinguishing with more complicated evolution. Additionally, levels significantly increased these patients. Mitochondrial dysfunction, redox state imbalance, impaired energy metabolism, chronic immune dysregulation are likely be main hallmarks years acute infection.

Language: Английский

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The tryptophan catabolite or kynurenine pathway in COVID-19 and critical COVID-19: a systematic review and meta-analysis

BMC Infectious Diseases, Journal Year: 2022, Volume and Issue: 22(1)

Published: July 15, 2022

Coronavirus disease 2019 (COVID-19) is accompanied by activated immune-inflammatory pathways and oxidative stress, which both induce indoleamine-2,3-dioxygenase (IDO), a key enzyme of the tryptophan (TRP) catabolite (TRYCAT) pathway. The aim this study was to systematically review meta-analyze status TRYCAT pathway, including levels TRP kynurenine (KYN) activity IDO, as measured ratio KYN/TRP.

Language: Английский

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Metabolomic characteristics and related pathways in patients with different severity of COVID-19: a systematic review and meta-analysis

Journal of Global Health, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 28, 2025

Despite advances in metabolomic research on COVID-19, existing studies have small sample sizes and few comprehensively described the metabolic characteristics of patients with COVID-19 at each stage. In this systematic review, we aimed to summarise similarities differences biomarkers different severity describe their stages. We retrieved from PubMed, Embase, Web Science, Cochrane Library published by October 2022. performed a meta-analysis untargeted targeted metabolomics data, using ratio means as effect size. compared changes metabolite levels between varying controls investigated sources heterogeneity through subgroup analyses meta-regression analysis. included 22 cohorts 21 studies, comprising 2421 participants, including healthy controls. conducted analysis 1058 metabolites study. The results indicated that, control group, 23 were associated mild cases (P < 0.05), 3 moderate 37 severe 0.05). Pathway enrichment revealed significant disturbances amino acid metabolism, aminoacyl-tRNA biosynthesis, primary bile pantothenate CoA tricarboxylic cycle, taurine hypotaurine nitrogen metabolism mild, moderate, disease. Additionally, found that stage exhibited unique patterns (all P 0.05) degree dysregulation progressively worsened increasing disease our indicate thereby providing new pathways for study pathogenesis, development precise treatment, formulation comprehensive strategies. PROSPERO: CRD42022369937.

Language: Английский

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Coronavirus disease 2019 (COVID‐19) update: From metabolic reprogramming to immunometabolism

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 94(10), P. 4611 - 4627

Published: June 11, 2022

The field of immunometabolism investigates and describes the effects metabolic rewiring in immune cells throughout activation fates these cells. Recently, it has been appreciated that plays an essential role progression viral infections, cancer, autoimmune diseases. Regarding COVID-19, aberrant response underlying diseases establishes two major respiratory pathologies, including acute distress syndrome (ARDS) or pneumonia-induced lung injury (ALI). Both innate adaptive immunity (T cell-based) were impaired course severe coronavirus 2 (SARS-CoV-2) infection. Current findings have deciphered macrophages (innate cells) are involved inflammatory seen COVID-19. It demonstrated system can change reprogramming some conditions, diseases, infectious disease, growing on COVID-19 allow exploration metabolites with immunomodulatory properties as future therapies to combat this hyperinflammatory response. elucidation exact mechanism reprograming could help apply more precise approaches initial diagnosis, prognosis, in-hospital therapy. This report discusses latest from host immunometabolic responses.

Language: Английский

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Metabolic Reprogramming in SARS-CoV-2 Infection Impacts the Outcome of COVID-19 Patients

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: July 11, 2022

Introduction Severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2) infection triggers inflammatory clinical stages that affect the outcome of patients with coronavirus disease 2019 (COVID-19). Disease severity may be associated a metabolic imbalance related to amino acids, lipids, and energy-generating pathways. The aim this study was characterize profile acids acylcarnitines in COVID-19 patients. A multicenter, cross-sectional carried out. total 453 individuals were classified by severity. Levels 11 31 acylcarnitines, succinylacetone serum samples analyzed electrospray ionization–triple quadrupole tandem mass spectrometry. Different clusters observed partial least squares discriminant analysis, phenylalanine, alanine, citrulline, proline, providing major contribution variability each cluster (variable importance projection &gt;1.5). In logistic models adjusted age, sex, type diabetes mellitus, hypertension, nutritional status, phenylalanine critical outcomes (odds ratio=5.3 (95% CI 3.16-9.2) severe vs. model, an area under curve 0.84 0.77-0.90). conclusion might progression. This work shows association patients, highlighting as potential biomarker

Language: Английский

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Untargeted analysis in post-COVID-19 patients reveals dysregulated lipid pathways two years after recovery

Frontiers in Molecular Biosciences, Journal Year: 2023, Volume and Issue: 10

Published: March 3, 2023

Introduction: Similar to what it has been reported with preceding viral epidemics (such as MERS, SARS, or influenza), SARS-CoV-2 infection is also affecting the human immunometabolism long-term consequences. Even underreporting, an accumulated of almost 650 million people have infected and 620 recovered since start pandemic; therefore, impact these consequences in world population could be significant. Recently, World Health Organization recognized post-COVID syndrome a new entity, guidelines are being established manage treat this condition. However, there still uncertainty about molecular mechanisms behind large number symptoms worldwide. Aims Methods: In study we aimed evaluate clinical lipidomic profiles (using non-targeted lipidomics) patients who had mild severe COVID-19 (acute phase, first epidemic wave); assessment was made two years after initial infection. Results: Fatigue (59%) musculoskeletal (50%) most relevant persistent. Functional analyses revealed that sterols, bile acids, isoprenoids, fatty esters were predicted metabolic pathways affected both post-COVID-19 patients. Principal Component Analysis showed differences between groups. Several species phosphatidylcholines sphingomyelins identified expressed higher levels compared controls. The paired analysis (comparing active 2 recovery) show 170 dysregulated features. relationship such dysregulations symptoms, point importance developing diagnostic therapeuthic markers based on cell signaling pathways.

Language: Английский

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Integrated Metabolic and Inflammatory Signatures Associated with Severity of, Fatality of, and Recovery from COVID-19

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: Feb. 28, 2023

COVID-19 is characterized by diverse clinical outcomes that include asymptomatic to mild manifestations or severe disease and death. Infection SARS-CoV-2 activates inflammatory metabolic responses drive protection pathology.

Language: Английский

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Distinct subsets of neutrophils crosstalk with cytokines and metabolites in patients with sepsis

iScience, Journal Year: 2023, Volume and Issue: 26(2), P. 105948 - 105948

Published: Jan. 7, 2023

Sepsis is a life-threatening condition caused by dysregulated host response to infection. Despite continued efforts understand the pathophysiology of sepsis, no effective therapies are currently available. While singular components aberrant immune have been investigated, comprehensive studies linking different data layers lacking. Using an integrated systems immunology approach, we evaluated neutrophil phenotypes and concomitant changes in cytokines metabolites patients with sepsis. Our findings identify differentially expressed mature immature subsets These correlate various proteins, metabolites, lipids, including pentraxin-3, angiopoietin-2, lysophosphatidylcholines, results enabled construction statistical model based on weighted multi-omics linear regression analysis for sepsis biomarker identification. could help inform early patient stratification treatment options, facilitate further mechanistic targeting trifecta surface marker expression, cytokines, metabolites.

Language: Английский

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Tryptophan catabolism via the kynurenine pathway regulates infection and inflammation: from mechanisms to biomarkers and therapies

Inflammation Research, Journal Year: 2024, Volume and Issue: 73(6), P. 979 - 996

Published: April 9, 2024

Language: Английский

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Unbalanced IDO1/IDO2 Endothelial Expression and Skewed Keynurenine Pathway in the Pathogenesis of COVID-19 and Post-COVID-19 Pneumonia

Biomedicines, Journal Year: 2022, Volume and Issue: 10(6), P. 1332 - 1332

Published: June 6, 2022

Despite intense investigation, the pathogenesis of COVID-19 and newly defined long syndrome are not fully understood. Increasing evidence has been provided metabolic alterations characterizing this group disorders, with particular relevance an activated tryptophan/kynurenine pathway as described in review. Recent histological studies have documented that, patients, indoleamine 2,3-dioxygenase (IDO) enzymes differentially expressed pulmonary blood vessels, i.e., IDO1 prevails early/mild pneumonia lung tissues from patients suffering COVID-19, whereas IDO2 is predominant severe/fatal cases. We hypothesize that necessary for a correct control vascular tone its deficiency might be related to syndrome’s evolution toward dysfunction. The complexity scenario discussed light possible therapeutic manipulations post-acute syndromes.

Language: Английский

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