Cancer Control, Journal Year: 2024, Volume and Issue: 31
Published: Jan. 1, 2024
This study aimed to investigate the role of discoidin domain receptor tyrosine kinase 1 (DDR1) in liver hepatocellular carcinoma (LIHC) and evaluate its prognostic value on patient response combination therapy.
Language: Английский
Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)
Published: Jan. 19, 2024
Abstract Hepatic stellate cell (HSC) activation is considered as a central driver of liver fibrosis and effective suppression HSC contributes to the treatment fibrosis. Circular RNAs (circRNAs) have been reported be important in tumor progression. However, contributions circRNAs remain largely unclear. The fibrosis-specific circRNA was explored by microarray cVIM (a derived from exons 4 8 vimentin gene mmu_circ_32994) selected research object. Further studies revealed that cVIM, mainly expressed cytoplasm, may act sponge for miR-122-5p miR-9-5p enhance expression type I TGF-β receptor (TGFBR1) TGFBR2 promotes TGF-β/Smad pathway, thereby accelerating progression Our results demonstrate vital role promoting provide fresh perspective on
Language: Английский
Citations
10
International Journal of Genomics, Journal Year: 2025, Volume and Issue: 2025(1)
Published: Jan. 1, 2025
Background:Helicobacter pylori (HP) is associated with the development of various stomach diseases, one major risk factors for adenocarcinoma (STAD). Methods: The HP infection score between tumor and normal groups was compared by single-sample gene set enrichment analysis (ssGSEA). key modules related to were identified weighted coexpression network (WGCNA), functional conducted on these module genes. Further, limma package used screen differentially expressed genes (DEGs) HP-positive HP-negative STAD. prognostic obtained construct riskscore model, performance model validated. correlation immune microenvironment (TIME) analyzed Spearman's method. single-cell atlas STAD delineated. mRNA expression levels verified using cells, migration invasion capacities cells evaluated wound healing assay transwell assay. Results: in group significantly higher than that group. purple royal blue showed STAD, enriched immune-related pathway. five (CTLA4, CPVL, EMB, CXCR4, FAM241A) screened from infection-related DEGs, which utilized establishing good robustness. Riskscore exhibited strong TIME Single-cell revealed CXCR4 highly Epithelial Cell 1, 2, parietal CTLA4, FAM241A, high silencing CPVL could suppress cells. Conclusion: This study established a based genes, provide reference prediction treatment targets
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: April 13, 2025
Transcatheter arterial chemoembolization (TACE) is a standard treatment for unresectable, intermediate, or advanced-stage hepatocellular carcinoma (HCC), especially in elderly patients. The modified 5-item frailty index (mFI-5) concise, comorbidity-based risk stratification tool proven to effectively predict adverse outcomes. However, the prognostic capacity of mFI-5 HCC patients after TACE unclear. As such, we retrospectively analyzed clinical data from (age ≥ 65 years) who received their first between November 2018 and 2020 at Department Interventional Radiology, Affiliated Hospital Xuzhou Medical University. was calculated based on presence five co-morbidities: congestive heart failure within 30 days prior surgery; insulin-dependent noninsulin-dependent diabetes mellitus; chronic obstructive pulmonary disease (COPD) pneumonia; partially dependent totally functional health status time hypertension requiring medication. Patients were divided into two groups scores: 2 ('frail') < ('non-frail'). primary outcomes overall survival (OS) progression-free (PFS).Among 143 patients, 97 group 46 group. median OS 40.0 months (95% confidence interval [CI]: 35.0-47.0) non-frail vs. 24.0 CI: 22-NA) frail (hazard ratio [HR] = 3.343, 95% 1.802-6.201, p .001). PFS 7.0 4.0-11.0) 3.0 2.0-9.0) (HR 1.507, 0.996-2.280, .053). Cox regression identified mFI-5, alpha-fetoprotein (AFP) as independent predictors OS. useful predictor long-term treated with TACE, suggesting that incorporating assessments can optimize strategies improve
Language: Английский
Citations
0
Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33682 - e33682
Published: June 26, 2024
AimsThis study explored the molecular and biologic mechanisms underlying association between circadian rhythm disorders (CRD) increased risk for hepatocellular carcinoma (HCC).BackgroundCRD are linked to HCC, but this limited.ObjectiveThe constructed validated a CRD related gene model as an independent prognostic factor providing insight into linking HCC identifying potential indicators efficacy of immunotherapy anticancer drugs. This helps provide important clues personalized treatment strategies patients.MethodsGene sets correlated with were obtained from Molecular Signatures Database (MSigDB) intersect differentially expressed genes (DEGs) tumor samples control in The Cancer Genome Atlas (TCGA) HCCDB18 Hepatocellular Carcinoma Cell DataBase (HCCDB). was developed by univariate Cox stepwise multivariate analysis. Immune checkpoint blockade (ICB) therapy drugs analyzed using immune dysfunction exclusion (TIDE) pRRophetic, respectively. Seurat determined cell type analyzing single-cell data, malignant cells identified Copykat. To detect mRNA levels model, quantitative real-time polymerase chain reaction (qRT-PCR) carried out.ResultsThe activity tissue significantly lower than that tissue. Subsequently, EZH2, IMPDH2, TYMS SERPINE1 selected construct which prognosis. Notably, low-risk patients had infiltration TIDE scores compared high-risk indicating low may derive more benefit immunotherapy. higher benign epithelial cells.ConclusionsThis presents reveal perspective dependent mechanism cancer, provides indicator understanding preclinical ICB
Language: Английский
Citations
2
Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)
Published: Aug. 12, 2024
Collagen triple helix repeat containing-1 (CTHRC1), an extracellular matrix protein, is highly expressed in hepatocellular carcinoma (HCC) and linked to poor prognosis. Nevertheless, the precise mechanism of CTHRC1 HCC unclear. Agena MassARRAY® Methylation Analysis assessed methylation level promoter region. Functional assays were conducted investigate effects knockdown Hep3B2.1 cells. RNA sequencing identified differentially genes lncRNAs associated with angiogenesis after knockdown. Furthermore, differential alternative splicing (AS) gene fusion events analyzed using rMATS Arriba. In cell lines, was hypomethylation. Downregulation inhibited proliferation, migration, invasion, blocked cells G1/S phase, promoted apoptosis. We obtained 34 mRNAs 7 between NC inhibitor groups. Additionally, we found 4 angiogenesis-related significantly correlated CTHRC1. RT-qPCR results showed that resulted aberrant expression CXCL6, LINC02127, AC020978.8. Moreover, role development may be events, like 12 AS 5 pairs genes. High hypomethylation promote development, involving angiogenesis, splicing, fusion.
Language: Английский
Citations
2
Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14
Published: July 5, 2024
Background This study aimed to assess the effectiveness and safety of vascular intervention combined with lenvatinib versus alone in treatment advanced hepatocellular carcinoma (HCC) portal vein tumor thrombus (PVTT), identify prognostic factors associated outcomes. Methods We conducted a retrospective analysis data from 92 patients HCC PVTT who were treated between February 2016 2023. Among them, 56 underwent (transarterial chemoembolization, TACE), while 36 received (TACE or hepatic arterial infusion chemotherapy [HAIC]) lenvatinib. The primary outcomes included progression-free survival (PFS), overall (OS), objective response rate (ORR). Survival rates estimated by Kaplan-Meier method, confounders adjusted using inverse probability weighting (IPTW). Prognostic determined through Cox regression model. Results median follow-up duration was 20.07 months (interquartile range: 6.41–25.36). combination therapy group had significantly longer PFS (11.00 vs. 5.00 months, P<0.05) OS (12.91 6.83 comparison monotherapy group, these findings remained consistent after IPTW matching. Moreover, showed higher ORR (55.56% 26.79%, based on mRECIST criteria. multivariate identified extrahepatic metastasis maximum diameter as risk for PFS, age, number, influenced OS. Combined emerged protective factor In hypertension most frequent adverse event, grade 3 4 events occurring rarely. Conclusion has demonstrated improved PVTT, its profile appears be acceptable. Adoption this strategy at an earlier stage may enhance patient
Language: Английский
Citations
1
Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34438 - e34438
Published: July 1, 2024
AimsTo analyze the expression of mitochondrial translational initiation factor 2 (MTIF2) and biological functions gene in hepatocellular carcinoma (HCC).BackgroundThe treatment HCC its prognostic prediction are limited by a lack comprehensive understanding molecular mechanisms HCC. OBJECTIVE: To determine cells expressing MTIF2 function MTIF2+ cell subpopulation.MethodsGene analysis on TIMER 2.0, UALCAN, GEPIA databases was performed to measure tissues. Cell clustering subgroups annotation were conducted based single-cell sequencing data paracancerous tissues Gene Expression Omnibus (GEO) database. different types analyzed. Further, pathways potentially regulated each type identified. In addition, protein-protein interaction (PPI) networks with genes developed. The assay verify effects superoxide dismutase-2 (SOD2) cells. Finally, we screened virtual drugs targeting SOD2 employing database screening, docking dynamics.ResultsMTIF2 showed remarkably high We identified total 10 between upregulated epithelial cells, macrophages, hepatocytes. More importantly, high-expressed mainly derived from hepatocytes, which reactive oxygen species (ROS) pathway significantly positively correlated MTIF2. PPI network, there unique pair ROS pathway. experiments that overexpression enhanced proliferative invasive capacities HCC, could synergize co-promote development dynamics simulations DB00183 maintained structural stability proteins during simulation process.ConclusionOur study confirmed might act regulate pathway, thereby affective progression
Language: Английский
Citations
1
Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34704 - e34704
Published: July 1, 2024
The prognosis and therapeutic response of patients with liver hepatocellular carcinoma (LIHC) can be predicted based on programmed cell death (PCD) as PCD plays a crucial role during tumor progression. We developed PCD-related gene signature to evaluate the for LIHC.
Language: Английский
Citations
1
Aging, Journal Year: 2024, Volume and Issue: unknown
Published: April 4, 2024
Background: Complex cellular signaling network in the tumor microenvironment (TME) could serve as an indicator for prognostic classification of hepatocellular carcinoma (HCC) patients. Methods: Univariate Cox regression analysis was performed to screen prognosis-related TME-related genes (TRGs), based on which HCC samples were clustered by running non-negative matrix factorization (NMF) algorithm. Furthermore, correlation between different molecular subtypes and immune cell infiltration level analyzed. Finally, a risk score (RS) model established LASSO analyses (CRA) using these TRGs. Functional enrichment gene set (GSEA). Results: patients divided into three (C1, C2, C3) 704 subtype C1 had significantly better OS than C2 C3. We selected 13 TRGs construct RS model. multivariate CRA showed that independently predict patients' prognosis. A nomogram integrating clinicopathologic features further created. also validated reliability according area under receiver operating characteristic (ROC) curve value, concordance index (C-index), decision analysis. The current findings demonstrated correlated with CD8+ T cells, monocytic lineage, myeloid dendritic cells. Conclusion: This study provided help classify their prognoses, contributing personalized treatments HCC.
Language: Английский
Citations
0
Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e30439 - e30439
Published: May 1, 2024
Hepatocellular carcinoma (HCC) is the main type of primary liver cancer. This study aimed to developed a basement membrane (BM) related lncRNAs risk signature evaluate prognosis HCC patients. We screened differentially expressed BM-related (DE-BMRlncRNAs) for evaluation, and identified six DE-BMRlncRNAs (AC072054.1, NUP50-DT, AC026412.3, AC109322.2, POLH-AS1 LINC00595) prognostic signature. patients were divided high or low according median score. Our model predicted that with higher score had worse prognosis. also created nomogram assist clinical decision-making clinicopathological features. Meanwhile, we confirmed expression in tissue cells. knockdown inhibited migration invasion In conclusion, established predictive based on BMRlncRNAs predict HCC. findings offer rationale further explore biomarkers
Language: Английский
Citations
0