OTX2 Homeoprotein Functions in Adult Choroid Plexus DOI Open Access
Anabelle Planques, Vanessa Oliveira Moreira,

David Benacom

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(16), P. 8951 - 8951

Published: Aug. 19, 2021

The choroid plexus is an important blood barrier that secretes cerebrospinal fluid, which essential for embryonic brain development and adult homeostasis. OTX2 homeoprotein a transcription factor critical remains highly expressed in plexus. Through RNA sequencing analyses of constitutive conditional knockdown mouse models, we reveal putative functional roles function, including cell signaling adhesion, show regulates the expression factors are secreted into notably transthyretin. We also Otx2 impacts immune stress responses, affects splicing, leading to changes mRNA isoforms proteins implicated oxidative response DNA repair. mass spectrometry analysis protein partners plexus, known non-cell-autonomous target regions, such as visual cortex subventricular zone, identify targets involved chromatin structure, processing. Thus, retains regulating function homeostasis throughout life.

Language: Английский

Structure and Function of the Blood–Brain Barrier (BBB) DOI
Fabienne Benz, Stefan Liebner

Handbook of experimental pharmacology, Journal Year: 2020, Volume and Issue: unknown, P. 3 - 31

Published: Jan. 1, 2020

Language: Английский

Citations

60
Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus DOI Creative Commons

Kim Hoa Ho,

Annarita Patrizi

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Feb. 8, 2021

Choroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles development, homeostasis and repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is popular useful technique for measuring gene expression changes also widely used ChP studies. However, the reliability of RT-qPCR data strongly dependent on choice reference genes, which are supposed to be stable across samples. In this study, we validated 12 well established housekeeping genes 2 independent experimental paradigms by using stability testing algorithms: BestKeeper, DeltaCq, geNorm NormFinder. Rer1 Rpl13a were identified as most throughout mouse while Hprt1 Rpl27 conditions sensory deprivation experiment. addition, Rpl13a, Tbp mutually among top five both experiments. Normalisation Ttr Otx2 levels different combinations demonstrated profound effect target expression. Our study emphasized importance validating selecting under specific conditions.

Language: Английский

Citations

46
Deconstructing the functional neuroanatomy of the choroid plexus: an ontogenetic perspective for studying neurodevelopmental and neuropsychiatric disorders DOI Creative Commons
Byron K.Y. Bitanihirwe, Paulo Lizano, Tsung‐Ung W. Woo

et al.

Molecular Psychiatry, Journal Year: 2022, Volume and Issue: 27(9), P. 3573 - 3582

Published: May 26, 2022

Abstract The choroid plexus (CP) is a delicate and highly vascularized structure in the brain comprised of dense network fenestrated capillary loops that help synthesis, secretion circulation cerebrospinal fluid (CSF). This unique neuroanatomical arachnoid villi stemming from frond-like surface projections—that protrude into lumen four cerebral ventricles—providing key source nutrients to parenchyma addition serving as ‘sink’ for central nervous system metabolic waste. In fact, functions CP are often described being analogous those liver kidney. Beyond forming barrier/interface between blood CSF compartments, has been identified modulator leukocyte trafficking, inflammation, cognition, circadian rhythm gut brain-axis. recent years, advances molecular biology techniques neuroimaging along with use sophisticated animal models have played an integral role shaping our understanding how CP–CSF changes relation maturation neural circuits during critical periods development. this article we provide ontogenetic perspective review experimental evidence implicating pathophysiology neurodevelopmental neuropsychiatric disorders.

Language: Английский

Citations

36
The molecular anatomy and functions of the choroid plexus in healthy and diseased brain DOI Creative Commons
Ingrid Kratzer, C. Joakim Ek, Helen B. Stolp

et al.

Biochimica et Biophysica Acta (BBA) - Biomembranes, Journal Year: 2020, Volume and Issue: 1862(11), P. 183430 - 183430

Published: Aug. 1, 2020

Language: Английский

Citations

48
Sex Differences in the Brain Transcriptome Related to Alcohol Effects and Alcohol Use Disorder DOI Creative Commons

Robert Hitzemann,

Susan E. Bergeson,

Ari Berman

et al.

Biological Psychiatry, Journal Year: 2021, Volume and Issue: 91(1), P. 43 - 52

Published: May 1, 2021

Language: Английский

Citations

40
Experimental approaches for manipulating choroid plexus epithelial cells DOI Creative Commons
Ahram Jang, Maria K. Lehtinen

Fluids and Barriers of the CNS, Journal Year: 2022, Volume and Issue: 19(1)

Published: May 26, 2022

Choroid plexus (ChP) epithelial cells are crucial for the function of blood-cerebrospinal fluid barrier (BCSFB) in developing and mature brain. The ChP is considered primary source regulator CSF, secreting many important factors that nourish It also performs CSF clearance functions including removing Amyloid beta potassium. As such, a promising target gene drug therapy neurodevelopmental neurological disorders central nervous system (CNS). This review describes current successful emerging experimental approaches targeting cells. We highlight methodological strategies to specifically these gain or loss vivo. cover both genetic models viral delivery systems. Additionally, several lines reporters access epithelia reviewed. Finally, we discuss exciting new approaches, such as chemical activation transplantation engineered elaborate on fundamental secretion outline paving way clinical applications.

Language: Английский

Citations

26
Regulation of choroid plexus development and its functions DOI Creative Commons
Petra Kompaníková, Vı́tězslav Bryja

Cellular and Molecular Life Sciences, Journal Year: 2022, Volume and Issue: 79(6)

Published: May 19, 2022

Abstract The choroid plexus (ChP) is an extensively vascularized tissue that protrudes into the brain ventricular system of all vertebrates. This highly specialized structure, consisting polarized epithelial sheet and underlying stroma, serves a spectrum functions within central nervous (CNS), most notably production cerebrospinal fluid (CSF). cells ChP have competence to tightly modulate biomolecule composition CSF, which acts as milieu functionally connecting with other structures. review aims eloquently summarize current knowledge about development ChP. We describe mechanisms control its early specification from roof plate followed by formation proliferative regions—cortical hem rhombic lips—feeding later Next, we summarized on maturation morphological cellular diversity. Furthermore, attempted currently available battery molecular markers mouse strains for research ChP, identified some technological shortcomings must be overcome accelerate field. Overall, principle this highlight intriguing surprisingly poorly known structure vital function whole CNS. believe our summary will increase interest in further studies aim principles guiding tissue.

Language: Английский

Citations

23
Brevican, Neurocan, Tenascin-C, and Tenascin-R Act as Important Regulators of the Interplay Between Perineuronal Nets, Synaptic Integrity, Inhibitory Interneurons, and Otx2 DOI Creative Commons

Cornelius Mueller-Buehl,

Jacqueline Reinhard,

Lars Roll

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: June 2, 2022

Fast-spiking parvalbumin interneurons are critical for the function of mature cortical inhibitory circuits. Most these neurons enwrapped by a specialized extracellular matrix (ECM) structure called perineuronal net (PNN), which can regulate their synaptic input. In this study, we investigated relationship between PNNs, interneurons, and distribution on cells in adult primary visual cortex (V1) quadruple knockout mice deficient ECM molecules brevican, neurocan, tenascin-C, tenascin-R. We used super-resolution structured illumination microscopy (SIM) to analyze PNN associated synapses. addition, examined calretinin interneuron populations. observed reduction number PNN-enwrapped clear disorganization V1. This was accompanied an imbalance excitatory synapses with increase elements along PNNs. Furthermore, reduced knockout, while do not wear did display differences number. Interestingly, found transcription factor Otx2 homeoprotein positive cell population also reduced. is crucial maturation, feedback loop parameters has been described. Collectively, data indicate important role tenascin-R regulating interplay distribution,

Language: Английский

Citations

23
Exploration of the mechanism by which icariin modulates hippocampal neurogenesis in a rat model of depression DOI Creative Commons
Can Yan, Lili Wu, Ning-Xi Zeng

et al.

Neural Regeneration Research, Journal Year: 2021, Volume and Issue: 17(3), P. 632 - 632

Published: Aug. 20, 2021

Icariin (ICA) has a significant capacity to protect against depression and hippocampal injury, but it cannot effectively cross the blood-brain barrier accumulate in brain. Therefore, mechanism by which ICA protects injury remains unclear. In this study, we performed proteomics analysis of cerebrospinal fluid investigate prevents dysfunctional neurogenesis depression. A rat model was established through exposure chronic unpredictable mild stress for 6 weeks, after 120 mg/kg administered subcutaneously every day. The results showed that alleviated depressive symptoms, learning memory dysfunction, neurogenesis, neuronal loss dentate gyrus rats with Neural stem cells from embryonic hippocampi were cultured media containing 20% each group then treated 100 μM corticosterone. addition largely prevented corticosterone-mediated inhibition proliferation differentiation. Fifty-two differentially expressed proteins regulated identified fluid. These mainly involved ribosome, PI3K-Akt signaling, interleukin-17 signaling pathways. Parallel reaction monitoring mass spectrometry Rps4x, Rps12, Rps14, Rps19, Hsp90b1, Hsp90aa1 up-regulated down-regulated ICA. contrast, HtrA1 findings suggest can prevent regulating expression certain found study approved Experimental Animal Ethics Committee Guangzhou University Chinese Medicine China March 2017.

Language: Английский

Citations

30
Development of the mammalian cortical hem and its derivatives: the choroid plexus, Cajal–Retzius cells and hippocampus DOI Creative Commons
Samantha A. Moore, Angelo Iulianella

Open Biology, Journal Year: 2021, Volume and Issue: 11(5)

Published: May 1, 2021

The dorsal medial region of the developing mammalian telencephalon plays a central role in patterning adjacent brain regions. This review describes development this specialized vertebrate brain, called cortical hem , and formation various cells structures it gives rise to, including choroid plexus, Cajal–Retzius hippocampus. We highlight ontogenic processes that create these different forebrain derivatives from their shared embryonic origin discuss key signalling pathways molecules influence hem. These include BMP, Wnt, FGF Shh acting with Homeobox factors to carve into district progenitor regions, which turn give dentate gyrus then link lateral ventricle plexus postnatal neurogenesis

Language: Английский

Citations

28