Long-range GABAergic projections contribute to cortical feedback control of sensory processing

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Dec. 20, 2020

Abstract Cortical sensory areas send excitatory projections back to earlier stage of processing. Here, we uncover for the first time existence a corticofugal inhibitory feedback between two areas, paralleling well-documented feedback. In olfactory system, reveal that subpopulation GABAergic neurons in anterior nucleus and piriform cortex target bulb. These long-range inputs synapse with both local output bulb neurons, mitral tufted cells. Optogenetic stimulation coupled vivo imaging network modeling showed activation these drives net subtractive inhibition spontaneous odor-evoked activity as well Further, cortical enhanced separation population odor responses cells, but not Targeted pharmacogenetic silencing axon terminals OB impaired discrimination similar mixtures. We propose here represents new circuit motif systems, involved refining processing perception.

Language: Английский

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Target-specific control of olfactory bulb periglomerular cells by GABAergic and cholinergic basal forebrain inputs

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: July 6, 2021

Abstract The olfactory bulb (OB), the first relay for odor processing, receives dense GABAergic and cholinergic long-range projections from basal forebrain (BF) nuclei that provide information about internal state behavioral context of animal. However, targets, impact dynamics these afferents are still unclear. I studied how BF synaptic inputs modulate activity in diverse subtypes periglomerular (PG) interneurons using optogenetic stimulation loose cell-attached or whole-cell patch-clamp recording OB slices adult mice. potently blocked PG cells firing except a minority calretinin-expressing which GABA release elicited spiking. Parallel excited previously overlooked cell subtype via activation M1 muscarinic receptors. Low frequency axons drove persistent thereby increasing tonic inhibition principal neurons. Taken together, findings suggest modality-specific can orchestrate glomeruli multiple, potentially independent, inhibitory excitatory target-specific pathways.

Language: Английский

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α2-Adrenergic modulation of Ih in adult-born granule cells in the olfactory bulb

Frontiers in Cellular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: Jan. 6, 2023

In the olfactory bulb (OB), a large population of axon-less inhibitory interneurons, granule cells (GCs), coordinate network activity and tune output principal neurons, mitral tufted (MCs), through dendrodendritic interactions. Furthermore, GCs undergo neurogenesis throughout life, providing source plasticity to neural OB. The function integration in OB are regulated by several afferent neuromodulatory signals, including noradrenaline (NA), state-dependent neuromodulator that plays crucial role regulation cortical task-specific decision processes. However, mechanisms which NA regulates GC not fully understood. Here, we show modulates hyperpolarization-activated currents (I h ) via activation α 2 -adrenergic receptors (ARs) adult-born (abGCs), thus directly acting on channels play essential roles regulating neuronal excitability oscillations brain. This modulation affects leading an enhancement lateral inhibition onto MCs. subthreshold resonance GCs, could affect temporal abGCs. Together, these results provide novel mechanism I can regulate

Language: Английский

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Parvalbumin-expressing basal forebrain neurons mediate learning from negative experience

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 31, 2023

Abstract Parvalbumin (PV)-expressing GABAergic neurons of the basal forebrain (BFPVNs) were proposed to serve as a rapid and transient arousal system. While they have well-documented role in regulation sleep-wake states, whether how BFPVNs participate mediating awake behaviors is not known. To address this, we performed bulk calcium imaging recorded single neuronal activity from horizontal band diagonal Broca (HDB) while mice performing an associative learning task. Genetically identified HDB responded with distinctive, phasic activation punishment. In contrast, reward only elicited slow delayed responses, stimuli predicting behavioral reinforcement (reward or punishment) followed by gradual increase BFPVN firing rates. Optogenetic inhibition during punishment impaired formation cue-outcome associations, suggesting causal these learning. Mapping input-output connectivity anterograde mono-transsynaptic retrograde tracing experiments showed that received strong inputs hypothalamus, septal complex median raphe region, synapsed on diverse cell types key structures limbic system including medial septum, retrosplenial cortex hippocampus. Bulk termination regions indicated broadcast information about aversive multiple downstream targets. We propose arousing effect recruited crucial functions behaviors.

Language: Английский

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Neuronal PAS domain 1 identifies a major subpopulation of wakefulness-promoting GABAergic neurons in basal forebrain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 11, 2023

ABSTRACT Here we describe a novel group of basal forebrain (BF) neurons expressing neuronal PAS domain 1 (Npas1), developmental transcription factor linked to neuropsychiatric disorders. Immunohistochemical staining in Npas1-cre-2A-TdTomato mice revealed BF Npas1 + are distinct from well-studied parvalbumin or cholinergic neurons. GAD67-GFP knock-in confirmed that the vast majority GABAergic, with minimal colocalization glutamatergic vGlut1-cre-tdTomato vGlut2-cre-tdTomato mice. The density was high, 5-6 times neighboring cholinergic, Anterograde tracing identified prominent projections brain regions involved sleep-wake control, motivated behaviors and olfaction such as lateral hypothalamus, habenula, nucleus accumbens shell, ventral tegmental area olfactory bulb. Chemogenetic activation light (inactive) period increased amount wakefulness latency sleep for 2-3 hr, due an increase long wake bouts short NREM bouts. Non-REM slow-wave (0-1.5 Hz) sigma (9-15 power, well spindle density, amplitude duration, were reduced, reminiscent findings several Together previous implicating stress responsiveness, anatomical effect activating them suggest possible role motivationally-driven stress-induced insomnia. Identification this major subpopulation GABAergic will facilitate studies their disorders, dementia other conditions involving BF. SIGNIFICANCE STATEMENT We characterize mouse subset more numerous than glutamate target areas arousal, motivation olfaction. Activation slow waves spindles reduced wake-promoting allow insomnia,

Language: Английский

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