Unraveling the Molecular Players at the Cholinergic Efferent Synapse of the Zebrafish Lateral Line DOI Creative Commons
Agustín E. Carpaneto Freixas, Marcelo J. Moglie,

Tais Castagnola

et al.

Journal of Neuroscience, Journal Year: 2020, Volume and Issue: 41(1), P. 47 - 60

Published: Nov. 17, 2020

The lateral line (LL) is a sensory system that allows fish and amphibians to detect water currents. LL responsiveness modulated by efferent neurons aid in distinguishing between external self-generated stimuli, maintaining sensitivity relevant cues. One component of the cholinergic, activation which inhibits afferent activity. hair cells (HCs) share structural, functional, molecular similarities with those cochlea, making them popular model for studying human hearing balance disorders. Because these commonalities, one could propose receptor at synapse α9α10 nicotinic acetylcholine (nAChR). However, identities players underlying ACh-mediated inhibition remain unknown. Surprisingly, through analysis single-cell expression studies situ hybridization, we describe α9, but not α10, subunits are enriched zebrafish HCs. Moreover, heterologous α9 indicates homomeric receptors functional exhibit robust ACh-gated currents blocked α-bungarotoxin strychnine. In addition, vivo Ca 2+ imaging on mechanically stimulated HCs show ACh elicits decrease evoked signals, regardless HC polarity. This effect both apamin, indicating coupling effects small-conductance -activated potassium (SKs) channels. Our results indicate an α9-containing (α9*) nAChR operates synapse. α9* nAChRs most likely leads hyperpolarization served SK SIGNIFICANCE STATEMENT mechanosensory shares mammalian cochlea. Thus, it has become accessible serving control cell (HC) activity have been identified. Here demonstrate that, different from organ vertebrate species, composed only Activation because opening These will further interpretation data obtained as cochlear

Language: Английский

Spatiotemporal EEG microstate analysis in drug-free patients with Parkinson's disease DOI Creative Commons
Chunguang Chu, Xing Wang, Lihui Cai

et al.

NeuroImage Clinical, Journal Year: 2019, Volume and Issue: 25, P. 102132 - 102132

Published: Dec. 20, 2019

The clinical diagnosis of Parkinson's disease (PD) is very difficult, especially in the early stage disease, because there no physiological indicator that can be referenced. Drug-free patients with PD are characterized by symptoms such as impaired motor function and cognitive decline, which was caused dysfunction brain's dynamic activities. indicators brain at an unmedicated condition may provide a valuable basis for later treatment. In order to find spatiotemporal characteristic markers PD, resting-state EEG microstate analysis used explore transient state whole 23 drug-free on sub-second timescale compared healthy controls. microstates reflect transiently stable topological structure characteristics, spatial classes temporal parameters insight into functional activities patients. further exploration relation between significant determine whether these could clinically assisted diagnosis. Therefore, we general linear model (GLM) relevance scales multiple patient attributes, Wilcoxon rank sum test quantify influencing factors parameters. Results revealed unique different from controls several other typical had differences normal control group, were reflected parameters, longer durations more occurrences one class Furthermore, correlation showed classes' symptoms, including decline. These results indicate have found quantifiable feature tags PD. Importantly, dynamics correlated represent recognize level. obtained deepen our understanding obtain some signatures auxiliary reference

Language: Английский

Citations

76
Lateral line ablation by ototoxic compounds results in distinct rheotaxis profiles in larval zebrafish DOI Creative Commons
Kyle C. Newton, Dovi Kacev, Simon Nilsson

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Jan. 21, 2023

Abstract The zebrafish lateral line is an established model for hair cell organ damage, yet few studies link mechanistic disruptions to changes in biologically relevant behavior. We used larval determine how damage via ototoxic compounds impact rheotaxis. Larvae were treated with CuSO 4 or neomycin disrupt function then exposed water flow stimuli. Their swimming behavior was recorded on video DeepLabCut and SimBA software track movements classify rheotaxis behavior, respectively. Lateral line-disrupted fish performed rheotaxis, but they swam greater distances, shorter durations, angular variance than controls. Furthermore, spectral decomposition analyses confirmed that lesioned exhibited compound-specific behavioral profiles distinct the magnitude, frequency, cross-correlation between fluctuations linear movements. Our observations demonstrate input needed hold their station efficiently reveals commonly lesion methods have unique effects

Language: Английский

Citations

26
Neuromodulatory Regulation of Behavioral Individuality in Zebrafish DOI Creative Commons
Carlos Pantoja,

Adam Hoagland,

Elizabeth C. Carroll

et al.

Neuron, Journal Year: 2016, Volume and Issue: 91(3), P. 587 - 601

Published: July 8, 2016

Language: Английский

Citations

85
Synaptically silent sensory hair cells in zebrafish are recruited after damage DOI Creative Commons
Qiu-Xiang Zhang,

Suna Li,

Hiu-Tung C. Wong

et al.

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: April 11, 2018

Analysis of mechanotransduction among ensembles sensory hair cells in vivo is challenging many species. To overcome this challenge, we used optical indicators to investigate collections intact zebrafish. Our imaging reveals a previously undiscovered disconnect between hair-cell mechanosensation and synaptic transmission. We show that saturating mechanical stimuli able open mechanically gated channels are unexpectedly insufficient evoke vesicle fusion the majority cells. Although synaptically silent, latent can be rapidly recruited after damage, demonstrating they competent. Therefore silent may an important reserve acts maintain function. results demonstrate unidentified level complexity sculpting transmission from periphery.

Language: Английский

Citations

69
The Descending Diencephalic Dopamine System Is Tuned to Sensory Stimuli DOI Creative Commons

Sebastian Reinig,

Wolfgang Driever, Aristides B. Arrenberg

et al.

Current Biology, Journal Year: 2017, Volume and Issue: 27(3), P. 318 - 333

Published: Jan. 12, 2017

Language: Английский

Citations

68
Sound exposure dynamically induces dopamine synthesis in cholinergic LOC efferents for feedback to auditory nerve fibers DOI Creative Commons
Jingjing Sherry Wu, Eunyoung Yi, Marco Manca

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Jan. 24, 2020

Lateral olivocochlear (LOC) efferent neurons modulate auditory nerve fiber (ANF) activity using a large repertoire of neurotransmitters, including dopamine (DA) and acetylcholine (ACh). Little is known about how individual neurotransmitter systems are differentially utilized in response to the ever-changing acoustic environment. Here we present quantitative evidence rodents that dopaminergic LOC input ANFs dynamically regulated according animal’s recent experience. Sound exposure upregulates tyrosine hydroxylase, an enzyme responsible for synthesis, cholinergic intrinsic neurons, suggesting might at times co-release ACh DA. We further demonstrate down-regulates ANF firing rates by reducing both hair cell release rate size synaptic events. Collectively, our results suggest can undergo on-demand re-specification re-calibrate activity, adjust gain cell/ANF synapses, possibly protect these synapses from noise damage.

Language: Английский

Citations

64
The genetic vulnerability to cisplatin ototoxicity: a systematic review DOI Creative Commons
Evangelia Tserga,

Tara Nandwani,

Niklas K. Edvall

et al.

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: March 5, 2019

Abstract Ototoxicity is one of the major side-effects platinum-based chemotherapy, in particular cisplatin (cis-diammine dichloroplatinum II). To our knowledge, no systematic review has previously provided a quantitative summary estimate impact genetics upon risk developing hearing loss. We searched Embase, Medline, ASSIA, Pubmed, Scopus, and Web Science, for studies documenting genetic ototoxicity patients with cancer treated cisplatin. Titles/abstracts full texts were reviewed inclusion. Meta-analytic estimates (Odds Ratio) from pooled data calculated that have been repeated twice or more. The search identified 3891 papers, which 30 included. majority retrospective (44%), ranging n = 39 to 317, some including only younger than 25 years age (33%), on both genders (80%). most common cancers involved osteosarcoma (53%), neuroblastoma (37%), prostate (17%) reproductive (10%). Most performed genotyping, though 5 genome-wide association studies. Nineteen single-nucleotide polymorphisms (SNPs) 15 genes more twice. Meta-analysis group indicated rs1872328 ACYP2 , plays role calcium homeostasis, increases by 4.61 (95% CI: 3.04–7.02; N 696, p < 0.0001) as well LRP2 rs4668123 shows cumulated Odds Ratio 3.53 1.48–8.45; 118, 0.0059), could not be evidenced individual Despite evidence heterogeneity across studies, these meta-analytic results are consistent view predisposition chemotherapy mediated ototoxicity. These new findings informative encourage screening order identify greater vulnerability loss, condition having potentially large quality life. More needed, larger sample size, additional markers ototoxic associated investigate polygenic risks, where multiple may exacerbate side-effects.

Language: Английский

Citations

60
Water Waves to Sound Waves: Using Zebrafish to Explore Hair Cell Biology DOI
Sarah B. Pickett, David W. Raible

Journal of the Association for Research in Otolaryngology, Journal Year: 2019, Volume and Issue: 20(1), P. 1 - 19

Published: Jan. 11, 2019

Language: Английский

Citations

56
Transmission Disrupted: Modeling Auditory Synaptopathy in Zebrafish DOI Creative Commons
Katie S. Kindt, Lavinia Sheets

Frontiers in Cell and Developmental Biology, Journal Year: 2018, Volume and Issue: 6

Published: Sept. 11, 2018

Sensorineural hearing loss is the most common form of in humans, and results from either dysfunction hair cells, sensory receptors sound, or neurons that innervate cells. A specific type sensorineural loss, referred to as auditory synaptopathy, occurs when cells are able detect sound but fail transmit stimuli at hair-cell synapse. Auditory synaptopathy can originate genetic alterations specifically disrupt synapse function. Additionally, environmental factors such noise exposure leave intact result synapses, represent an acquired synaptopathy. The zebrafish model has emerged a valuable system for studies function, In this review, we describe experimental tools have been developed study synapses zebrafish. We discuss how genetics helped identify define roles synaptic proteins crucial highlight contributed our understanding formation addition, also work used pharmacological mimic noise-induced excitotoxicity cellular mechanisms underlying damage loss. Lastly, future could enhance pathological processes both This knowledge critical order develop therapies protect repair contacts.

Language: Английский

Citations

49
Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells DOI Creative Commons

Young Rae Ji,

Sunita Warrier, Tao Jiang

et al.

eLife, Journal Year: 2018, Volume and Issue: 7

Published: April 19, 2018

The orientation of hair bundles on top sensory cells (HCs) in neuromasts the lateral line system allows fish to detect direction water flow. Each neuromast shows arranged two opposing directions and each afferent neuron innervates only HCs same orientation. Previously, we showed that this opposition is established by expression Emx2 half HCs, where it mediates bundle reversal (<xref ref-type="bibr" rid="bib15">Jiang et al., 2017</xref>). Here, show also regulates neuronal selection: neurons innervate either Emx2-positive or negative HCs. In emx2 knockout gain-of-function neuromasts, all are unidirectional innervation patterns physiological responses dependent presence absence Emx2. Our results indicate directional selectivity distinct processes: regulating selecting targets.

Language: Английский

Citations

38