Localized semi-nonnegative matrix factorization (LocaNMF) of widefield calcium imaging data

PLoS Computational Biology, Journal Year: 2020, Volume and Issue: 16(4), P. e1007791 - e1007791

Published: April 13, 2020

Widefield calcium imaging enables recording of large-scale neural activity across the mouse dorsal cortex. In order to examine relationship these signals resulting behavior, it is critical demix recordings into meaningful spatial and temporal components that can be mapped onto well-defined brain regions. However, no current tools satisfactorily extract different regions in individual mice a data-driven manner, while taking account mouse-specific preparation-specific differences. Here, we introduce Localized semi-Nonnegative Matrix Factorization (LocaNMF), method efficiently decomposes widefield video data allows us directly compare multiple by outputting localized functional are significantly more interpretable than traditional decomposition techniques. Moreover, provides natural subspace correlation maps dynamics behaviors, mice, experimental conditions, identification task- movement-related

Language: Английский

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Chronic, cortex-wide imaging of specific cell populations during behavior

Nature Protocols, Journal Year: 2021, Volume and Issue: 16(7), P. 3241 - 3263

Published: June 2, 2021

Language: Английский

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In vivo lensless microscopy via a phase mask generating diffraction patterns with high-contrast contours

Nature Biomedical Engineering, Journal Year: 2022, Volume and Issue: 6(5), P. 617 - 628

Published: March 7, 2022

The simple and compact optics of lensless microscopes the associated computational algorithms allow for large fields view refocusing captured images. However, existing techniques cannot accurately reconstruct typical low-contrast images optically dense biological tissue. Here we show that imaging tissue in vivo can be achieved via an optical phase mask designed to create a point spread function consisting high-contrast contours with broad spectrum spatial frequencies. We built prototype microscope incorporating 'contour' used it image calcium dynamics cortex live mice (over field about 16 mm2) freely moving Hydra vulgaris, as well microvasculature oral mucosa volunteers. low cost, small form factor capability microscopy may open up clinical uses, especially difficult-to-reach areas body.

Language: Английский

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Through the looking glass: A review of cranial window technology for optical access to the brain

Journal of Neuroscience Methods, Journal Year: 2021, Volume and Issue: 354, P. 109100 - 109100

Published: Feb. 15, 2021

Deciphering neurologic function is a daunting task, requiring understanding the neuronal networks and emergent properties that arise from interactions among single neurons. Mechanistic insights into require tools simultaneously assess both neuron activity consequent mesoscale output. The development of cranial window technologies, in which skull thinned or replaced with synthetic optical interface, has enabled monitoring subcellular to resolution awake, behaving animals when coupled advanced microscopy techniques. Here we review recent achievements appraise relative merits each design discuss future research design.

Language: Английский

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An analog of psychedelics restores functional neural circuits disrupted by unpredictable stress

Molecular Psychiatry, Journal Year: 2021, Volume and Issue: 26(11), P. 6237 - 6252

Published: May 25, 2021

Abstract Psychological stress affects a wide spectrum of brain functions and poses risks for many mental disorders. However, effective therapeutics to alleviate or revert its deleterious effects are lacking. A recently synthesized psychedelic analog tabernanthalog (TBG) has demonstrated anti-addictive antidepressant potential. Whether TBG can rescue stress-induced affective, sensory, cognitive deficits, how it may achieve such by modulating neural circuits, remain unknown. Here we show that in mice exposed unpredictable mild (UMS), administration single dose decreases their anxiety level rescues deficits sensory processing as well flexibility. Post-stress treatment promotes the regrowth excitatory neuron dendritic spines lost during UMS, baseline neuronal activity, enhances whisking-modulation activity somatosensory cortex. Moreover, calcium imaging head-fixed performing whisker-dependent texture discrimination task shows novel textures elicit responses from greater proportion neurons cortex than do familiar textures. Such differential response is diminished UMS restored TBG. Together, our study reveals on cortical circuit patterns demonstrate combats detrimental basal stimulus-dependent networks.

Language: Английский

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Wide-Field Calcium Imaging of Dynamic Cortical Networks during Locomotion

Cerebral Cortex, Journal Year: 2021, Volume and Issue: 32(12), P. 2668 - 2687

Published: Sept. 15, 2021

Motor behavior results in complex exchanges of motor and sensory information across cortical regions. Therefore, fully understanding the cerebral cortex's role requires a mesoscopic-level description regions engaged, their functional interactions, how these interactions change with behavioral state. Mesoscopic Ca2+ imaging through transparent polymer skulls mice reveals elevated activation dorsal cortex during locomotion. Using correlations between time series fluorescence from 28 (nodes) obtained using spatial independent component analysis (sICA), we examined changes connectivity rest to locomotion goal state that facilitate Both transitions show marked increases correlation among most nodes. However, once steady continued is reached, many nodes, including primary somatosensory decreases correlations, while retrosplenial anterior nodes secondary increases. These highlight cortex, representing on return rest.

Language: Английский

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REM sleep is associated with distinct global cortical dynamics and controlled by occipital cortex

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Nov. 12, 2022

Abstract The cerebral cortex is spontaneously active during sleep, yet it unclear how this global cortical activity spatiotemporally organized, and whether such not only reflects sleep states but also contributes to state switching. Here we report that cortex-wide calcium imaging in mice revealed distinct stage-dependent spatiotemporal patterns of activity, modulation could regulate In particular, elevated activation the occipital regions (including retrosplenial visual areas) became dominant rapid-eye-movement (REM) sleep. Furthermore, pontogeniculooccipital (PGO) wave-like was associated with transitions REM optogenetic inhibition strongly promoted deep by suppressing NREM-to-REM transition. Thus, whereas subcortical networks are critical for initiating maintaining wakefulness states, plays an role controlling states.

Language: Английский

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Pyramidal cell types drive functionally distinct cortical activity patterns during decision-making

Nature Neuroscience, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 23, 2023

Abstract Understanding how cortical circuits generate complex behavior requires investigating the cell types that comprise them. Functional differences across pyramidal neuron (PyN) have been observed within areas, but it is not known whether these local extend throughout cortex, nor additional emerge when larger-scale dynamics are considered. We used genetic and retrograde labeling to target tract, intratelencephalic corticostriatal projection neurons measured their cortex-wide activity. Each PyN type drove unique neural dynamics, both at scales. Cortical activity optogenetic inactivation during an auditory decision task revealed distinct functional roles. All PyNs in parietal cortex were recruited perception of stimulus, but, surprisingly, tract had largest causal role. In frontal all required for accurate choices showed choice tuning. Our results reveal rich, cell-type-specific shape perceptual decisions.

Language: Английский

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Rapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 27(1), P. 148 - 158

Published: Nov. 30, 2023

Language: Английский

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Cortex-wide neural dynamics predict behavioral states and provide a neural basis for resting-state dynamic functional connectivity

Cell Reports, Journal Year: 2023, Volume and Issue: 42(6), P. 112527 - 112527

Published: May 26, 2023

Although resting-state functional magnetic resonance imaging (fMRI) studies have observed dynamically changing brain-wide networks of correlated activity, fMRI's dependence on hemodynamic signals makes results challenging to interpret. Meanwhile, emerging techniques for real-time recording large populations neurons revealed compelling fluctuations in neuronal activity across the brain that are obscured by traditional trial averaging. To reconcile these observations, we use wide-field optical mapping simultaneously record pan-cortical and awake, spontaneously behaving mice. Some components clearly represent sensory motor function. However, particularly during quiet rest, strongly fluctuating patterns diverse regions contribute greatly interregional correlations. Dynamic changes correlations coincide with arousal state. Simultaneously acquired hemodynamics depict similar brain-state-dependent correlation shifts. These support a neural basis dynamic fMRI, while highlighting importance study

Language: Английский

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