Mitigation and use of biofilms in space for the benefit of human space exploration DOI Creative Commons

Yo-Ann Vélez Justiniano,

Darla M. Goeres,

Elizabeth L. Sandvik

et al.

Biofilm, Journal Year: 2023, Volume and Issue: 5, P. 100102 - 100102

Published: Jan. 6, 2023

Biofilms are self-organized communities of microorganisms that encased in an extracellular polymeric matrix and often found attached to surfaces. widely present on Earth, diverse sometimes extreme environments. These microbial have been described as recalcitrant or protective when facing adversity environmental exposures. On the International Space Station, biofilms were human-inhabited environments a multitude hardware Moreover, studies identified phenotypic genetic changes under microgravity conditions including microbe surface colonization pathogenicity traits. Lack consistent research microgravity-grown can lead deficient understanding altered behavior space. This could subsequently create problems engineered systems negatively impact human health crewed spaceflights. It is especially relevant long-term remote space missions will lack resupply service. Conversely, also known benefit plant growth essential for (i.e., gut microbiome). Eventually, may be used supply metabolic pathways produce organic inorganic components useful sustaining life celestial bodies beyond Earth. article explore what currently about identify gaps aerospace industry's knowledge should filled order mitigate leverage advantage spaceflight.

Language: Английский

Timing of antibiotic administration determines the spread of plasmid-encoded antibiotic resistance during microbial range expansion DOI Creative Commons
Yinyin Ma, Josep Ramoneda, David R. Johnson

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: June 14, 2023

Abstract Plasmids are the main vector by which antibiotic resistance is transferred between bacterial cells within surface-associated communities. In this study, we ask whether there an optimal time to administer antibiotics minimize plasmid spread in new genotypes during community expansion across surfaces. We address question using consortia of Pseudomonas stutzeri strains, where one resistance-encoding donor and other a potential recipient. allowed strains co-expand surface administered at different times. find that transfer transconjugant proliferation have unimodal relationships with timing administration, they reach maxima intermediate These result from interplay probabilities loss. Our study provides mechanistic insights into plasmids microbial communities identifies administration as important determinant.

Language: Английский

Citations

17
The winding journey of conjugative plasmids toward a novel host cell DOI Creative Commons
Nathan Fraikin, Agathe Couturier, Christian Lesterlin

et al.

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 78, P. 102449 - 102449

Published: March 2, 2024

Horizontal transfer of plasmids by conjugation is a fundamental mechanism driving the widespread dissemination drug resistance among bacterial populations. The successful colonization new host cell necessitates plasmid to navigate through series sequential steps, each dependent on specific or factors. This review explores recent advancements in comprehending cellular and molecular mechanisms that govern transmission, establishment, long-term maintenance. Adopting plasmid-centric perspective, we describe critical steps bottlenecks plasmid's journey toward cell, encompassing exploration contact initiation, invasion, establishment control, assimilation.

Language: Английский

Citations

6
Subinhibitory antibiotic concentrations promote the horizontal transfer of plasmid-borne resistance genes from Klebsiellae pneumoniae to Escherichia coli DOI Creative Commons

Manlin Ding,

Zi Ye, Lu Liu

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 7, 2022

Horizontal gene transfer plays an important role in the spread of antibiotic resistance, which plasmid-mediated conjugation is most mechanism. While sub-minimal inhibitory concentrations (sub-MIC) antibiotics could promote frequency, mechanism by sub-MIC levels affect frequency not clear. Here, we used Klebsiella pneumoniae SW1780 carrying multi-drug resistance plasmid pSW1780-KPC as donor strain, to investigate effects sub-MICs meropenem (MEM), ciprofloxacin (CIP), cefotaxime (CTX), and amikacin (AK) on conjugational from Escherichia coli J53. Our results showed that frequencies increased significantly treating strain with MEM, CIP, CTX AK. Transfer at conditions a Galleria mellonella were higher than vitro. To expression metabolic effects, RT-qPCR LC-MS-based metabolome sequencing performed. Transcript T4SS genes virB1, virB2, virB4, virB8, conjugation-related traB, traK, traE, traL upregulated exposure CTX, Metabolome revealed nine differentially regulated metabolites. findings are early warning for wide assessment roles resistance.

Language: Английский

Citations

26
Adaptive laboratory evolution of Escherichia coli W enhances gamma-aminobutyric acid production using glycerol as the carbon source DOI
Kangsan Kim, Chen Hou, Donghui Choe

et al.

Metabolic Engineering, Journal Year: 2021, Volume and Issue: 69, P. 59 - 72

Published: Nov. 11, 2021

Language: Английский

Citations

28
The distribution of fitness effects of plasmid pOXA-48 in clinical enterobacteria DOI
Ariadna Fernández-Calvet, Laura Toribio-Celestino, Aída Alonso-del Valle

et al.

Microbiology, Journal Year: 2023, Volume and Issue: 169(7)

Published: July 28, 2023

Antimicrobial resistance (AMR) in bacteria is a major public health problem. The main route for AMR acquisition clinically important the horizontal transfer of plasmids carrying genes. allow to survive antibiotics, but they also entail physiological alterations host cell. Multiple studies over last few years have indicated that these can translate into fitness cost when antibiotics are absent. However, due technical limitations, most based on analysing new associations between and generated vitro , we know very little about effects their native bacterial hosts. In this study, used CRISPR-Cas9-tool selectively cure from clinical enterobacteria overcome limitation. Using approach, were able study carbapenem plasmid pOXA-48 35 pOXA-48-carrying isolates recovered hospitalized patients. Our results revealed produces variable across collection wild-type enterobacterial strains naturally plasmid, ranging costs benefits. Importantly, was only associated with significant reduction four out clones, produced no changes great majority isolates. suggest produce neutral hosts, helping explain prevalence natural microbial communities.

Language: Английский

Citations

12
Exclusion Systems Preserve Host Cell Homeostasis and fitness, Ensuring Successful Dissemination of Conjugative Plasmids and Associated Resistance Genes DOI Creative Commons
Agathe Couturier, Nathan Fraikin, Christian Lesterlin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

ABSTRACT Plasmid conjugation is a major driver of antibiotic resistance dissemination in bacteria. In addition to genes required for transfer and maintenance, conjugative plasmids encode exclusion systems that prevent host cells from acquiring identical or redundant plasmids. Despite their ubiquity, the biological impact these remains poorly understood. Here, we investigate importance mechanism plasmid dynamics bacterial physiology at single-cell level. Using real-time microscopy, directly visualize how absence results unregulated self-transfer, causing continuous repeated exchange among cells. This runaway severely compromises cell integrity, viability, fitness, largely undescribed phenomenon termed lethal zygosis. We demonstrate zygosis associated with membrane stress, activation SOS response potential reactivation SOS-inducible prophages, as well chromosome replication segregation defects. study highlights maintain homeostasis by limiting transfer. Paradoxically, this restriction critical successful conferring selective advantage, which explains evolutionary conservation underscores role spread pathogenic

Language: Английский

Citations

0
Genomic Insights into CRISPR-Harboring Plasmids in the Klebsiella Genus: Distribution, Backbone Structures, Antibiotic Resistance, and Virulence Determinant Profiles DOI
Jinzhao Long, Jiangfeng Zhang,

Yanyan Xi

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 15, 2023

CRISPR systems are often encoded by many prokaryotes as adaptive defense against mobile genetic elements (MGEs), but several MGEs also recruit components to perform additional biological functions. Type IV-A identified in Klebsiella plasmids, yet the distribution, characterization, and role of these plasmids carrying whole genus remain unclear. Here, we performed large-scale comparative analysis using publicly available plasmid genomes.

Language: Английский

Citations

10
IncC plasmid genome rearrangements influence the vertical and horizontal transmission tradeoff in Escherichia coli DOI Creative Commons
Margaux Allain, Thibaut Morel‐Journel, Bénédicte Condamine

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: 68(10)

Published: Aug. 28, 2024

It has been shown that an evolutionary tradeoff between vertical (host growth rate) and horizontal (plasmid conjugation) transmissions contributes to global plasmid fitness. As conjugative IncC plasmids are important for the spread of multidrug resistance (MDR), in a broad range bacterial hosts, we investigated two multidrug-resistant according their backbones MDR-region rearrangements, upon entry into new host. We observed genome deletions after conjugation three diverse natural

Language: Английский

Citations

3
The metabolic burden associated with plasmid acquisition: An assessment of the unrecognized benefits to host cells DOI

Heather D. Curtsinger,

Sofía Martínez‐Absalón,

Yuchang Liu

et al.

BioEssays, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Bacterial conjugation, wherein DNA is transferred between cells through direct contact, highly prevalent in complex microbial communities and responsible for spreading myriad genes related to human environmental health. Despite their importance, much remains unknown regarding the mechanisms driving spread persistence of these plasmids situ. Studies have demonstrated that transferring, acquiring, maintaining a plasmid imposes significant metabolic burden on host. Simultaneously, emerging evidence suggests presence conjugative can also provide both obvious unexpected benefits host local community. Combined, this highlights continuous cost‐benefit tradeoff at population level, likely contributing overall abundance long‐term persistence. Yet, while burdens causes, are widely studied, attendant potential advantages less clear. Here, we summarize current perspectives plasmids’ then highlight lesser‐appreciated yet critical plasmid‐mediated may provide. We argue largely unexplored fundamental theory populations engineering applications therefore warrants further detailed study.

Language: Английский

Citations

3
Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria DOI Creative Commons

Julia R. Savelieva,

Daria A. Kondratieva, Maria V. Golikova

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(1), P. 238 - 238

Published: Jan. 20, 2025

Background/Objectives: Plasmid-mediated resistance is a significant mechanism that contributes to the gradual decrease in efficacy of antibiotics from various classes, including carbapenems. The aim this study investigate frequency transfer carbapenemase-encoding plasmids K. pneumoniae E. coli and P. aeruginosa. Methods: Matings were performed on agar with subsequent isolation transconjugant, recipient, donor colonies. conjugation (CF) minimum inhibitory concentrations (MICs) meropenem determined for PCR-confirmed transconjugants. A pharmacodynamic was conducted using hollow-fiber infection model transconjugant order evaluate its viability presence therapeutic meropenem. Results: CF pneumoniae-K. similar pneumoniae-E. higher MIC donor. MICs transconjugants (0.25–4 μg/mL) compared recipients (0.03–0.06 μg/mL). aeruginosa did not acquire pneumoniae. In experiments, an 2 mg/L within “susceptibility range”, failed respond treatment. Conclusions: between falls range. permissiveness pneumoniae, i.e., same species, observed. Conjugation occur borderline susceptibility may pose potential threat

Language: Английский

Citations

0