iScience,
Journal Year:
2022,
Volume and Issue:
25(8), P. 104819 - 104819
Published: Aug. 1, 2022
Ovarian
aging
precedes
that
of
any
other
mammalian
organ
and
is
the
primary
cause
female
age-related
infertility.
The
biological
mechanisms
responsible
for
ovarian
remain
unclear.
Previous
studies
have
been
limited
by
their
use
bulk
RNA-sequencing,
which
masks
dynamic
heterogeneous
nature
ovary.
In
this
study,
we
spatially
resolved
transcriptomic
landscape
ovaries
from
young
aged
outbred
mice.
total,
defined
eight
main
cell
populations,
all
were
characterized
significant
changes
between
samples.
Further
sub-cluster
analysis
revealed
separate
transcriptomes
distinct
granulosa
populations
found
in
versus
mice,
addition
to
an
oocyte
population
completely
absent
mouse
ovaries.
This
study
provides
a
new
perspective
on
using
spatial
transcriptomics
achieve
deeper
understanding
localization
cell-population-specific
underlying
fertility
decline.
Human Reproduction Update,
Journal Year:
2024,
Volume and Issue:
30(5), P. 614 - 647
Published: June 28, 2024
Chemotherapy-associated
ovarian
damage
(CAOD)
is
one
of
the
most
feared
short-
and
long-term
side
effects
anticancer
treatment
in
premenopausal
women.
Accumulating
detailed
data
show
that
different
chemotherapy
regimens
can
lead
to
disturbance
hormone
levels,
reduced
or
lost
fertility,
an
increased
risk
early
menopause.
Previous
studies
have
often
focused
on
direct
chemotherapeutic
drugs
follicles,
such
as
DNA
damage-mediated
apoptotic
death
primordial
follicle
burnout.
Emerging
evidence
has
revealed
imbalance
microenvironment
during
chemotherapy.
The
provides
nutritional
support
transportation
signals
stimulate
growth
development
ovulation,
corpus
luteum
formation.
close
interaction
between
follicles
determine
function.
Therefore,
designing
novel
precise
strategies
manipulate
may
be
a
new
strategy
protect
function
Journal of Ovarian Research,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: May 31, 2024
Abstract
In
women
who
are
getting
older,
the
quantity
and
quality
of
their
follicles
or
oocytes
decline.
This
is
characterized
by
decreased
ovarian
reserve
function
(DOR),
fewer
remaining
oocytes,
lower
oocytes.
As
more
choose
to
delay
childbirth,
decline
in
fertility
associated
with
age
has
become
a
significant
concern
for
modern
women.
The
oocyte
key
indicator
aging.
Many
studies
suggest
that
age-related
changes
energy
metabolism
may
impact
quality.
Changes
affect
adenosine
5'-triphosphate
(ATP)
production,
but
how
related
products
proteins
influence
remains
largely
unknown.
review
focuses
on
aging
its
potential
quality,
as
well
therapeutic
strategies
partially
metabolism.
research
aims
enhance
our
understanding
metabolism,
identification
biomarkers
treatment
methods.
Nature Cell Biology,
Journal Year:
2024,
Volume and Issue:
26(7), P. 1124 - 1138
Published: June 20, 2024
Abstract
Women
are
born
with
all
of
their
oocytes.
The
oocyte
proteome
must
be
maintained
minimal
damage
throughout
the
woman’s
reproductive
life,
and
hence
for
decades.
Here
we
report
that
ovarian
proteostasis
involves
extreme
protein
longevity.
Mouse
ovaries
had
more
extremely
long-lived
proteins
than
other
tissues,
including
brain.
These
diverse
functions,
in
mitochondria,
cytoskeleton,
chromatin
proteostasis.
stable
resided
not
only
oocytes
but
also
somatic
cells.
Our
data
suggest
mammals
increase
longevity
enhance
by
chaperones
cellular
antioxidants
to
maintain
female
germline
long
periods.
Indeed,
aggregation
did
age
proteasome
activity
decay.
However,
increasing
cannot
fully
block
senescence.
Large-scale
profiling
~8,890
revealed
a
decline
many
network
aging
ovary,
accompanied
massive
remodeling,
which
eventually
leads
fertility
decline.
Developmental Cell,
Journal Year:
2024,
Volume and Issue:
59(4), P. 529 - 544.e5
Published: Jan. 30, 2024
During
human
fetal
development,
sex
differentiation
occurs
not
only
in
the
gonads
but
also
adjacent
developing
reproductive
tract.
However,
while
cellular
composition
of
male
and
female
is
well
described,
that
tract
remains
poorly
characterized.
Here,
we
performed
single-cell
transcriptomics
on
together
with
from
first
second
trimesters,
highlighting
morphological
molecular
changes
during
differentiation.
We
validated
different
cell
populations
compared
signatures
between
as
sexes,
to
identify
conserved
sex-specific
features.
Together,
our
study
provides
insights
into
gonadogenesis
development
beyond
gonads.
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: March 11, 2024
The
genetics
of
sex
determination
varies
across
taxa,
sometimes
even
within
a
species.
Major
domesticated
strains
zebrafish
(
Danio
rerio
),
including
AB
and
TU,
lack
strong
genetic
determining
locus,
but
more
recently
derived
from
nature,
like
Nadia
(NA),
possess
ZZ
male/ZW
female
chromosomal
sex-determination
system.
fish
pass
through
juvenile
ovary
stage,
forming
oocytes
that
survive
in
become
females
die
males.
To
understand
mechanisms
gonad
development
NA
zebrafish,
we
studied
histology
single
cell
transcriptomics
developing
ZW
fish.
developed
by
22
days
post-fertilization
(dpf)
directly
formed
testes,
avoiding
phase.
Gonads
some
WW
fish,
however,
died
as
the
became
testis,
mimicking
suggesting
gynogenetically
strain
is
chromosomally
WW.
Single-cell
RNA-seq
19dpf
gonads
showed
similar
types
germ
cells,
precursors
gonadal
support
steroidogenic
interstitial/stromal
immune
consistent
with
bipotential
gonad.
In
contrast,
scRNA-seq
30dpf
revealed
cells
had
transcriptomes
characteristic
testicular
Sertoli,
Leydig,
while
granulosa
theca
oocytes.
Hematopoietic
vascular
were
both
genotypes.
These
results
show
initially
develop
gonad;
factor
on
W
chromosome,
or
fewer
than
two
Z
chromosomes,
essential
to
initiate
oocyte
development;
without
factor,
doses,
into
testes
passing
stage.
Sex
TU
mimics
loss
chromosome
during
domestication.
Genetic
analysis
will
facilitate
our
understanding
evolution
mechanisms.
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(5), P. 585 - 585
Published: May 15, 2024
Epithelial
ovarian
cancer
(EOC)
is
one
of
the
most
aggressive
forms
gynaecological
malignancies.
Survival
rates
for
women
diagnosed
with
OC
remain
poor
as
patients
are
advanced
disease.
Debulking
surgery
and
platinum-based
therapies
current
mainstay
treatment.
However,
despite
achieving
initial
remission,
a
significant
portion
will
relapse
because
innate
acquired
resistance,
at
which
point
disease
considered
incurable.
In
view
this,
novel
detection
strategies
therapeutic
approaches
needed
to
improve
outcomes
survival
patients.
this
review,
we
summarize
our
knowledge
genetic
landscape
molecular
pathways
underpinning
its
many
subtypes.
By
examining
explored
in
preclinical
clinical
settings,
highlight
importance
decoding
how
single
convergent
alterations
co-exist
drive
progression
resistance
treatments.
We
also
propose
that
core
signalling
such
PI3K
MAPK
play
critical
roles
origin
diverse
subtypes
can
become
new
targets
combination
known
DNA
damage
repair
development
tailored
more
effective
anti-cancer
International Journal of Molecular Sciences,
Journal Year:
2020,
Volume and Issue:
21(21), P. 8147 - 8147
Published: Oct. 31, 2020
Polycystic
ovary
syndrome
(PCOS)
is
a
common
endocrinopathy,
characterized
by
chronic
anovulation,
hyperandrogenism,
and
multiple
small
subcapsular
cystic
follicles
in
the
during
ultrasonography,
affects
5–10%
of
women
reproductive
age.
PCOS
frequently
associated
with
insulin
resistance
(IR)
accompanied
compensatory
hyperinsulinemia
and,
therefore,
presents
an
increased
risk
type
2
diabetes
mellitus
(DM).
The
pathophysiology
unclear,
many
hypotheses
have
been
proposed.
Among
these
hypotheses,
IR
hyperandrogenism
may
be
two
key
factors.
first
line
treatment
includes
lifestyle
changes
body
weight
reduction.
Achieving
5–15%
reduction
improve
PCOS-associated
hormonal
abnormalities.
For
who
desire
pregnancy,
clomiphene
citrate
(CC)
front-line
for
ovulation
induction.
Twenty
five
percent
fail
to
ovulate
spontaneously
after
three
cycles
CC
treatment,
which
called
CC-resistant
PCOS.
women,
there
are
strategies
rate,
including
medical
surgical
approaches.
various
approaches,
one
particular
method,
laparoscopic
ovarian
drilling
(LOD),
has
proposed
as
alternative
treatment.
LOD
results
overall
spontaneous
rate
30–90%
final
pregnancy
rates
13–88%.
These
benefits
more
significant
Although
intra-
post-operative
complications
sequelae
always
important,
we
believe
that
better
understanding
pathophysiological
and/or
molecular
mechanisms
provide
rationale
this
procedure.
LOD,
mediated
mainly
thermal
effects,
produces
series
morphological
biochemical
changes.
include
formation
artificial
holes
very
thick
cortical
wall,
loosening
dense
hard
destruction
subsequently
decreased
amount
theca
granulosa
cells,
stromal
tissue
subsequent
development
transient
but
purulent
acute
inflammatory
reactions
initiate
immune
response,
continuing
leakage
or
drainage
“toxic”
follicular
fluid
immature
growth-ceased
pre-antral
follicles.
All
factors
contribute
decreasing
local
systemic
androgen
levels,
following
apoptosis
process
atresia;
re-starting
normal
recruitment,
development,
maturation,
finally,
normalization
“hypothalamus–pituitary–ovary”
axis
ovulation.
detailed
systematic
comprehensively
reviewed
current
article.
Proceedings of the National Academy of Sciences,
Journal Year:
2021,
Volume and Issue:
118(20)
Published: May 12, 2021
Müllerian
inhibiting
substance
(MIS/AMH),
produced
by
granulosa
cells
of
growing
follicles,
is
an
important
regulator
folliculogenesis
and
follicle
development.
Treatment
with
exogenous
MIS
in
mice
suppresses
development
prevents
ovulation.
To
investigate
the
mechanisms
which
inhibits
development,
we
performed
single-cell
RNA
sequencing
whole
neonatal
ovaries
treated
at
birth
analyzed
postnatal
day
6,
coinciding
first
wave
growth.
We
identified
distinct
transcriptional
signatures
associated
responses
ovarian
cell
types.
treatment
inhibited
proliferation
granulosa,
surface
epithelial,
stromal
types
ovary
elicited
a
unique
signature
quiescence
cells.
In
addition
to
decreasing
number
preantral
found
that
uncoupled
maturation
germ
conclusion,
suppressed
proliferation,
imposing
quiescent
state,
preventing
differentiation.
