CCL2: An important cytokine in normal and pathological pregnancies: A review

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 6, 2023

C-C motif ligand 2 (CCL2), also known as monocytic chemotactic protein 1 (MCP-1), is an integral factor which recruits macrophages for the immune response. Together with its receptors (e.g., CCR2, ACKR1, and ACKR2), they exert noticeable influences on various diseases of different systems. At maternal-fetal interface, CCL2 detected to be expressed in trophoblasts, decidual tissue, myometrium, others. Meanwhile, existing reports have determined a series physiological regulators CCL2, functions maintaining normal recruitment immunocytes, tissue remodeling, angiogenesis. However, abnormal levels been reported associated adverse pregnancy outcomes such spontaneous abortion, preeclampsia preterm labor. In this review, we concentrate expression at well precise regulatory mechanisms classic signaling pathways, reveal multidimensional aspects pregnancy.

Language: Английский

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Deciphering maternal-fetal cross-talk in the human placenta during parturition using single-cell RNA sequencing

Science Translational Medicine, Journal Year: 2024, Volume and Issue: 16(729)

Published: Jan. 10, 2024

Labor is a complex physiological process requiring well-orchestrated dialogue between the mother and fetus. However, cellular contributions communications that facilitate maternal-fetal cross-talk in labor have not been fully elucidated. Here, single-cell RNA sequencing (scRNA-seq) was applied to decipher signaling human placenta during term labor. First, atlas of established, demonstrating maternal fetal cell types underwent changes transcriptomic activity Cell most affected by were stromal decidual cells chorioamniotic membranes (CAMs) myeloid placenta. Cell-cell interaction analyses showed CAM placental participated labor-driven signaling, including collagen, C-X-C motif ligand (CXCL), tumor necrosis factor (TNF), galectin, interleukin-6 (IL-6) pathways. Integration scRNA-seq data with publicly available bulk placenta-derived signatures could be monitored circulation throughout gestation Moreover, comparative analysis revealed mirrored those spontaneous preterm birth. Furthermore, we demonstrated early gestation, labor-specific, detected women destined undergo birth, either intact or prelabor ruptured membranes. Collectively, our findings provide insight into parturition suggest can aid development noninvasive biomarkers for prediction

Language: Английский

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Innate and Adaptive Immune Systems in Physiological and Pathological Pregnancy

Biology, Journal Year: 2023, Volume and Issue: 12(3), P. 402 - 402

Published: March 3, 2023

The dynamic immunological changes occurring throughout pregnancy are well-orchestrated and important for the success of pregnancy. One key immune adaptations is maternal tolerance towards semi-allogeneic fetus. In this review, we provide a comprehensive overview what known about innate adaptive in role(s) specific cells during physiological pathological Alongside this, provided details remaining questions challenges, as well future perspectives growing field research. Understanding that occur can inform potential strategies on treatments optimal health neonate pregnant individual both after

Language: Английский

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Omega-3 fatty acid supply in pregnancy for risk reduction of preterm and early preterm birth

American Journal of Obstetrics & Gynecology MFM, Journal Year: 2023, Volume and Issue: 6(2), P. 101251 - 101251

Published: Dec. 7, 2023

This clinical practice guideline on the supply of omega-3 docosahexaenoic acid and eicosapentaenoic in pregnant women for risk reduction preterm birth early was developed with support from several medical-scientific organizations, is based a review available strong evidence randomized trials formal consensus process. We concluded following. Women childbearing age should obtain at least 250 mg/d docosahexaenoic+eicosapentaenoic diet or supplements, pregnancy an additional intake ≥100 to 200 acid. Pregnant low and/or blood levels have increased birth. Thus, they receive approximately 600 1000 acid, alone, given that this dosage showed significant controlled trials. preferably begin second trimester (not later than 20 weeks' gestation) continue until 37 gestation childbirth if before gestation. Identification inadequate achievable by set standardized questions intake. Docosahexaenoic measurement another option identify status, but further standardization laboratory methods appropriate cutoff values needed. Information how achieve be provided their partners.

Language: Английский

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High amniotic fluid fractalkine and MIP-1β levels are associated with intrauterine growth restriction: a prospective cohort study

TURKISH JOURNAL OF MEDICAL SCIENCES, Journal Year: 2024, Volume and Issue: 54(1), P. 280 - 290

Published: Jan. 1, 2024

Proinflammatory chemokines have been shown to play crucial roles in implantation, spiral artery invasion, and thefetomaternal immunological response. In this context, we investigated the levels of fractalkine (CX3CL1) chemokine CC motifligand 4 (CCL4 or MIP-1β) maternal serum amniotic fluids pregnant women with intrauterine growth restriction (IUGR).Materials methods: This prospective cohort study was carried out at Fırat University Obstetrics Clinic between January 1, 2022 andJuly 2022. Group (G) 1: The control group consisted 40 who underwent elective cesarean section (CS) 38–40weeks gestation. G2: A total IUGR 28–37 weeks gestation were included group. Levelsof tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interferon-gamma (IFN-γ), hypoxia-inducible factor-1 alpha (HIF-1α), macrophage inflammatory protein-1 (MIP-1β), measured fluid samplesobtained during CS.Results: When age compared, no statistically significant difference observed G1 G2 (p = 0.374). Thenumber gravidity found be higher compared 0.003). mean gestational week statisticallyhigher < 0.001). Maternal MIP-1β 0.03) IFN-γ 0.006) G1. birth weight baby(p 0.001) umbilical cord blood gas pH value HIF-1α 0.001), 0.001),MIP-1β TNF-α 0.007), IL-1β 0.007) G2.Conclusion: Elevated proinflammatory factors, including MIP-1β, along factors such asTNF-α, IL-1β, IFN-γ, as well increased fluid, are associated restriction(IUGR) attributed a hypoxic environment.

Language: Английский

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Obstructive Sleep Apnea in Pregnancy: A Comprehensive Review of Maternal and Fetal Implications

Neurology International, Journal Year: 2024, Volume and Issue: 16(3), P. 522 - 532

Published: May 7, 2024

Obstructive sleep apnea (OSA) is a prevalent yet underdiagnosed condition in pregnancy, associated with various maternal and fetal complications. This review synthesizes the current evidence on epidemiology, pathophysiology, neurological consequences of OSA along potential management strategies. Articles were sourced from PubMed, EMBASE, Cochrane databases until 2023. Our comprehensive highlights that incidence increases during pregnancy due to physiological changes such as weight gain hormonal fluctuations. linked gestational hypertension, pre-eclampsia, diabetes, adverse outcomes intrauterine growth restriction preterm birth. Continuous positive airway pressure (CPAP) therapy remains most effective strategy for pregnant women OSA. However, adherence CPAP often suboptimal. underscores importance early recognition, timely diagnosis, improve both outcomes. Future research should focus enhancing screening strategies improving this population.

Language: Английский

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Inflammatory mechanisms of preterm labor and emerging anti-inflammatory interventions

Cytokine & Growth Factor Reviews, Journal Year: 2024, Volume and Issue: 78, P. 50 - 63

Published: July 20, 2024

Preterm birth is a major public health concern, requiring deeper understanding of its underlying inflammatory mechanisms and to develop effective therapeutic strategies. This review explores the complex interaction between inflammation preterm labor, highlighting pivotal role dysregulation in triggering premature delivery. The immunological environment pregnancy, characterized by fragile balance immune tolerance resistance, disrupted leading pathological response. Feto-maternal infections, among other pro-inflammatory stimuli, trigger activation toll-like receptors production mediators, promoting uterine contractility cervical ripening. Emerging anti-inflammatory therapeutics offer promising approaches for prevention targeting key pathways. From TLR-4 antagonists chemokine interleukin receptor antagonists, these interventions aim modulate prevent adverse pregnancy outcomes. In conclusion, comprehensive labor crucial development targeted hope reducing incidence improving neonatal

Language: Английский

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Extracellular Vesicles-mediated recombinant IL-10 protects against ascending infection-associated preterm birth by reducing fetal inflammatory response

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 4, 2023

Background Fetal inflammatory response mediated by the influx of immune cells and activation pro-inflammatory transcription factor NF-κB in feto-maternal uterine tissues is major determinant infection-associated preterm birth (PTB, live births &lt; 37 weeks gestation). Objective To reduce incidence PTB minimizing inflammation, extracellular vesicles (EVs) were electroporetically engineered to contain anti-inflammatory cytokine interleukin (IL)-10 (eIL-10), their efficacy was tested an ascending model infection (vaginal administration E. coli ) induced mouse models. Study design EVs (size: 30-170 nm) derived from HEK293T electroporated with recombinant IL-10 at 500 volts 125 Ω, 6 pulses generate eIL-10. eIL-10 structural characters (electron microscopy, nanoparticle tracking analysis, ExoView [size cargo content] functional properties (co-treatment macrophage LPS eIL-10) assessed. test efficacy, CD1 mice vaginally inoculated (10 10 CFU) subsequently treated either PBS, (500ng) or Gentamicin (10mg/kg) a combination eIL-10+gentamicin. maternal fetal after treatment conducted suspension Cytometer Time Flight (CyTOF) using transgenic that express red fluorescent TdTomato (mT+) cells. Results Engineered structurally functionally stable showed reduced proinflammatory production challenged vitro . Maternal µg/kg body weight) crossed barriers delay -induced deliver pups term. Delay associated inflammation (immune cell infiltration histologic chorioamnionitis, activation, production). Conclusions safe, stable, specific, delayed over 72 hrs delivered pups. The delivery drugs overcomes limitations in-utero interventions. Protecting eliminates need for amniotic its efficacy.

Language: Английский

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Roles of immune microenvironment in the female reproductive maintenance and regulation: novel insights into the crosstalk of immune cells

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 28, 2023

Female fertility decline is an accumulative consequence caused by complex factors, among them, the disruption of immune profile in female reproduction stands out as a crucial contributor. Presently, effects microenvironment (IME) on reproductive process have attracted increasing attentions for their dynamic but precisive roles. Immunocytes including macrophages, dendritic cells, T B cells and neutrophils, with diverse subpopulations well high plasticity functioned dynamically through indirect intercellular communication via specific cytokine release transduced molecular signal networks or direct cell-cell contact to maintain stability been unveiled. The each stage has also meticulously Especially, application single-cell sequencing (scRNA-seq) technology this reveals distribution map which gives novel insight homeostasis IME provides research direction better exploring role reproduction. Here, we provide all-encompassing overview latest advancements modulation within context process. Our approach involves structuring our summary accordance physiological sequence encompassing gonadogenesis, folliculogenesis ovaries, ovulation fallopian tubes, subsequent stages embryo implantation development uterus. overarching objective construct comprehensive portrayal (IME), thereby accentuating pivotal played governing intricate journey. Additionally, emphasize pressing need heightened attention directed towards strategies that focus interventions process, ultimate aim enhancing fertility.

Language: Английский

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Docosahexaenoic Acid (DHA) and Eicosapentaenoic Acid (EPA)—Should They Be Mandatory Supplements in Pregnancy?

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1471 - 1471

Published: July 3, 2024

Docosahexaenoic acid (DHA) and eicosapentaenoic (EPA) are essential fatty acids for the human body. Seafood microalgae most important sources of omega-3 acids. Supplementation with 200 mg/day DHA during pregnancy breastfeeding has been suggested women infants in countries low seafood consumption. Maternal concentration EPA was associated cord blood breast milk. High concentrations were identified at level retinal photoreceptors neuronal cell membranes. It observed that supplementation had beneficial effects on neurological development fetus infant by improving language, memory, attention, hand coordination, affecting sleep patterns, visual acuity. Beneficial also maternal intake breastfeeding. may reduce risk preterm birth but preeclampsia low-risk pregnancies. Women childbearing age should have an 250 + from their diet or supplements. To premature birth, pregnant must additionally receive least 100–200 mg every day. is recommended starts before 20 weeks pregnancy. mother identified, such as reduction postpartum depression symptoms, decrease cardiovascular risk, anti-inflammatory role.

Language: Английский

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