Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: March 28, 2023

Abstract The reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is a cheaper and faster testing alternative for detecting SARS-CoV-2. However, high false-positive rate due to misamplification one of the major limitations. To overcome misamplifications, we developed colorimetric fluorometric RT-LAMP assays using five LAMP primers, instead six. gold-standard RT-PCR technique verified assays' performance. Compared other primer sets with six primers (N, S, RdRp), E-ID1 set, including performed superbly on both assays. sensitivity was 89.5% 92.2%, respectively, limit detection 20 copies/µL. had specificity 97.2% an accuracy 94.5%, while obtained 99% 96.7%, respectively. No evident even after 120 min, which crucial success this technique. These findings are important support use in healthcare systems fighting COVID-19.

Language: Английский

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TMPRSS2 polymorphism (rs12329760) and the severity of the COVID-19 in Iranian population

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(2), P. e0281750 - e0281750

Published: Feb. 16, 2023

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has been responsible for the recent pandemic since early 2020. Due to wide range of clinical symptoms this disease, from asymptomatic severe and critical forms, it seems that genetic differences among patients, along with other factors (such as gender, age, underlying diseases), can explain part variation in disease symptoms. The TMPRSS2 enzyme plays a vital role stages interaction SARS-CoV-2 host cells by facilitating viral entry. There is polymorphism gene, called rs12329760(C T) missense variant, which causes replacement valine methionine protein at position 160. present study investigated association between genotype severity 2019 (COVID-19) Iranian patients. 251 COVID-19 patients (151 mild 100 symptoms) was detected on genomic DNA extracted patients’ peripheral blood via ARMS-PCR method. Our results showed significant minor T allele ( P-value = 0.043) under dominant additive inheritance model. In conclusion, rs12329760 gene risk form contrast most previous studies variant European ancestry populations suggested protective allele. reiterate ethnic-specific alleles hidden unknown complexity behind susceptibility. However, further are needed address complex mechanisms determining severity.

Language: Английский

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Persisting Shadows: Unraveling the Impact of Long COVID-19 on Respiratory, Cardiovascular, and Nervous Systems

Infectious Disease Reports, Journal Year: 2023, Volume and Issue: 15(6), P. 806 - 830

Published: Dec. 15, 2023

The coronavirus disease 2019 (COVID-19), instigated by the zoonotic Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), rapidly transformed from an outbreak in Wuhan, China, into a widespread global pandemic. A significant post-infection condition, known as 'long- COVID-19' (or simply COVID'), emerges substantial subset of patients, manifesting with constellation over 200 reported symptoms that span multiple organ systems. This also 'post-acute sequelae SARS-CoV-2 infection' (PASC), presents perplexing clinical picture far-reaching implications, often persisting long after acute phase. While initial research focused on immediate pulmonary impact virus, recognition COVID-19 multiorgan disruptor has unveiled gamut protracted and severe health issues. review summarizes primary effects COVID respiratory, cardiovascular, nervous It delves mechanisms underlying these impacts underscores critical need for comprehensive understanding COVID's pathogenesis.

Language: Английский

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Ultrasensitive and fast detection of SARS-CoV-2 using RT-LAMP without pH-dependent dye

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 20, 2024

Language: Английский

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Trajectory of post-COVID brain fog, memory loss, and concentration loss in previously hospitalized COVID-19 survivors: the LONG-COVID-EXP multicenter study

Frontiers in Human Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: Nov. 9, 2023

This study aimed to apply Sankey plots and exponential bar for visualizing the trajectory of post-COVID brain fog, memory loss, concentration loss in a cohort previously hospitalized COVID-19 survivors.A sample 1,266 patients due during first wave pandemic were assessed at 8.4 (T1), 13.2 (T2), 18.3 (T3) months after hospital discharge. They asked about presence following self-reported cognitive symptoms: fog (defined as self-perception sluggish or fuzzy thinking), unusual forgetfulness), not being able maintain attention). We symptoms that individuals had experienced previously, they attributed them acute infection. Clinical hospitalization data collected from medical records.The revealed prevalence was 8.37% (n = 106) T1, 4.7% 60) T2, 5.1% 65) T3, whereas 14.9% 189) 11.4% 145) 12.12% 154) T3. Finally, decreased 6.86% 87) 4.78% 2.63% 33) The recovery curves show decreasing trend, indicating these recovered years regression models did reveal any record associated with long term.The use shows fluctuating evolution In addition, decrease No risk factors identified this cohort.

Language: Английский

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SARS‐CoV‐2 Activated Peripheral Blood Mononuclear Cells (PBMCs) Do Not Provoke Adverse Effects in Trophoblast Spheroids

American Journal of Reproductive Immunology, Journal Year: 2025, Volume and Issue: 93(1)

Published: Jan. 1, 2025

ABSTRACT Problem Although it is still uncertain whether Severe Acute Respiratory Coronavirus (SARS‐CoV‐2) placental infection and vertical transmission occur, inflammation during early pregnancy can have devastating consequences for gestation itself the growing fetus. If how SARS‐CoV‐2‐specific immune cells negatively affect placenta functionality unknown. Method of study We stimulated peripheral blood mononuclear (PBMCs) from women reproductive age with SARS‐CoV‐2 peptides cocultured them trophoblast spheroids (HTR‐8/SVneo JEG‐3) to dissect if SARS‐CoV‐2‐activated interfere functionality. The activation cytokine profile PBMCs were determined using multicolor flow cytometry. was assessed microscopy, enzyme‐linked immunosorbent assay (ELISA), RT‐qPCR. Results S M significantly activated (monocytes, NK cells, T memory subsets) induced upregulation proinflammatory cytokines, such as IFNγ. did not impact viability, growth rate, invasion capabilities spheroids. Furthermore, hormonal production hCG by JEG‐3 compromised upon coculture PBMCs. mRNA transcript levels genes involved in spheroid functional pathways also dysregulated after coculture. Conclusions Together, findings our vitro model, although fully representative vivo conditions, strongly support claim that interaction at fetal–maternal interface does This goes hand recommendation vaccinating pregnant their first trimester.

Language: Английский

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Genetics of Long COVID: Exploring the Molecular Drivers of Persistent Pulmonary Vascular Disease Symptoms

Infectious Disease Reports, Journal Year: 2025, Volume and Issue: 17(1), P. 15 - 15

Published: Feb. 13, 2025

Background/ Objectives: Long COVID or post-acute sequelae of SARS-CoV-2 infection (PASC) are symptoms that manifest despite passing the acute phase. These manifestations encompass a wide range symptoms, most common being fatigue, shortness breath, and cognitive dysfunction. Genetic predisposition is clearly involved in susceptibility individuals to developing these persistent variation severity forms. This review summarizes role genetic factors gene polymorphisms development major pulmonary vascular disorders associated with long COVID. Methods: A comprehensive current literature was conducted examine contributions complications following infection. Studies investigating linked hypertension, thromboembolism, endothelialitis were reviewed summarized. Results: Findings show specific variants contribute increased patients. Variants endothelial dysfunction, coagulation pathways, inflammatory responses have been implicated hypertension thromboembolic events. predispositions influencing integrity immune appear influence disease progression. Conclusions: Understanding mechanisms could pave way for targeted therapeutic interventions alleviate burden on patients experiencing

Language: Английский

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Three-Year Follow-Up of COVID-19 Cases in District of Constance, Germany. A Prospective, Controlled Cohort Study (FSC19-KN)

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(5), P. 1439 - 1439

Published: Feb. 21, 2025

Background and Objectives: Long-term sequalae of viral diseases, especially after infections with SARS-CoV-2 (COVID-19), can induce multi-organ involvement, as around 65 million people worldwide report persistent symptoms that go far beyond the acute course. Studies indicate early virus variants pose a higher risk developing post-COVID-19 conditions. The primary aim this study was to investigate possible long-term effects based on hospitalization rates associated clinical events in patients infected over an observational period three years initial infection. Secondarily, investigation health-related quality life functional status performed. Methods Materials: presented designed prospective, controlled cohort follow up COVID-19 cases district Konstanz, Germany (FSC19-KN). positive group included subjects who had infection between March December 2020. control did not have infection, evidenced by negative antibody test. As endpoint, respective related admission diagnosis during from January 2021 until July 2024 were analyzed. outcomes measured SF-36 questionnaire Post-COVID-19 Functional Status (PCFS) secondary endpoint. Results: During observation inclusion study, rate differ significantly two groups initially non-infected (cumulative events, verum 57 45, OR 1.24, CI 0.83; 1.85, p = 0.30). However, life, sub scores SARS-CoV-2-positive subjects, achieved lower results, except for dimension ‘energy fatigue’, which still scores. Conclusions: Mild no significant impact Yet, reported reduced outcomes. Ultimately, only score fatigue’ registered differences both cohorts at end three-year period.

Language: Английский

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Mathematical modeling of a MoSe₂-based SPR biosensor for detecting SARS-CoV-2 at nM concentrations

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 28, 2025

The rapid and accurate detection of SARS-CoV-2 remains a critical challenge in biosensing technology, necessitating the development highly sensitive selective platforms. In this study, we present mathematical modeling approach to optimize MoSe₂-based Surface Plasmon Resonance (SPR) biosensor for detecting novel coronavirus at nM scale. Using Transfer Matrix Method (TMM), systematically biosensor’s structural parameters, including silver (Ag), silicon nitride (Si₃N₄), molybdenum diselenide (MoSe₂), thiol-tethered single-stranded DNA (ssDNA) layers, enhance sensitivity, accuracy, optical performance. results indicate that an optimized 45 nm Ag layer, 10 Si₃N₄ monolayer MoSe₂ configuration achieves resonance shift (Δθ) 0.3° 100 nM, with sensitivity 197.70°/RIU accuracy 5.24 × 10⁻ 2 . Additionally, incorporation ssDNA functionalization layer significantly enhances molecular recognition, lowering limit (LoD) 2.53 5 improving overall efficiency. Sys₅ (MoSe₂ + ssDNA) outperforms Sys₄ without terms specificity reliability, making it more suitable practical applications. These findings establish SPR as promising candidate detection, offering balance between high stability, selectivity, crucial effective viral diagnostics.

Language: Английский

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Anti-interferon armamentarium of human coronaviruses

Cellular and Molecular Life Sciences, Journal Year: 2025, Volume and Issue: 82(1)

Published: March 13, 2025

Abstract Cellular innate immune pathways are formidable barriers against viral invasion, creating an environment unfavorable for virus replication. Interferons (IFNs) play a crucial role in driving and regulating these cell-intrinsic antiviral mechanisms through the action of interferon-stimulated genes (ISGs). The host IFN response obstructs replication at every stage, prompting viruses to evolve various strategies counteract or evade this response. Understanding interplay between proteins IFN-mediated is essential developing anti-inflammatory strategies. Human coronaviruses (HCoVs), including SARS-CoV-2, MERS-CoV, SARS-CoV, seasonal coronaviruses, encode range that, shared distinct mechanisms, inhibit responses. Compounding issue, dysregulated early can lead hyper-inflammatory reaction later infection, resulting severe disease. This review provides brief overview HCoV detailed account its interaction with cellular regulated by IFN.

Language: Английский

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