Circular RNAs and Their Role in Exosomes DOI Creative Commons
Zeping Han, Huafang Chen,

Zhonghui Guo

et al.

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: April 28, 2022

As a novel class of endogenous non-coding RNAs discovered in recent years, circular (circRNAs) are highly conserved and stable covalently closed ring structures with no 5′-end cap or 3′-end poly(A) tail. CircRNAs formed by reverse splicing, mainly means noose structure intron complementary pairing. Exosomes tiny discoid vesicles diameter 40-100 nm that secreted cells under physiological pathological conditions. play an important role cell-cell communication carrying DNA, microRNAs, mRNAs, proteins circRNAs. In this review, we summarize the biological functions circRNAs exosomes, further reveal potential roles exosomal different diseases, providing scientific basis for diagnosis, treatment, prognosis wide variety diseases.

Language: Английский

Hsa_circRNA_0088036 acts as a ceRNA to promote bladder cancer progression by sponging miR-140-3p DOI Creative Commons
Jun Yang,

Manlong Qi,

Xiang Fei

et al.

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(4)

Published: April 8, 2022

Abstract Circular RNAs (circRNAs) are a class of non-coding that play vital roles in cancer biology. However, the potential role hsa_circRNA_0088036 bladder (BCa) remains unknown. Hsa_circRNA_0088036 was identified by microarray analysis and validated quantitative real-time polymerase chain reaction. Functional assays were conducted to confirm effects on growth, migration, invasion, tumorigenesis, metastasis BCa cells. The luciferase reporter assay RNA pull down performed investigate interactions between hsa_circRNA_0088036, miR-140-3p, forkhead box protein Q1 (FOXQ1). Upregulated expression tissues cell lines positively correlated with overall survival clinicopathologic characteristics. Knockdown inhibited invasion cells both vivo vitro. Mechanistically, could directly interact miR-140-3p act as miRNA sponge modulate FOXQ1 expression. proliferation, via miR-140-3p/FOXQ1 signaling, suggesting is biomarker therapeutic target for BCa.

Language: Английский

Citations

52
Roles and clinical application of exosomal circRNAs in the diagnosis and treatment of malignant tumors DOI Creative Commons
Dong Ye,

Mengdan Gong,

Yongqin Deng

et al.

Journal of Translational Medicine, Journal Year: 2022, Volume and Issue: 20(1)

Published: April 5, 2022

Abstract Exosomes are microvesicles secreted by cells. They contain a variety of bioactive substances with important roles in intercellular communication. Circular RNA (circRNA), type nucleic acid molecule found exosomes, forms covalently bonded closed loop without 5′ caps or 3′ poly(A) tails. It is structurally stable, widely distributed, and tissue specific. CircRNAs mainly act as microRNA sponges have regulatory gene expression; they superior to other non-coding RNAs molecular diagnostic markers drug treatment targets. Exosomal-derived circRNAs the body fluids tumor patients can modulate proliferation, invasion, metastasis, resistance. be used effective biomarkers for early non-invasive diagnosis prognostic evaluation tumors, also represent ideal targets precision therapeutic intervention. This review provides theoretical basis exploring applications exosomal malignant treatment. We describe biological functions occurrence development their potential utility treatment, possible mechanisms.

Language: Английский

Citations

44
Circular RNA as a Novel Biomarker and Therapeutic Target for HCC DOI Creative Commons

Hongwu Meng,

Ruowen Niu,

Cheng Huang

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(12), P. 1948 - 1948

Published: June 17, 2022

Circular RNA (circRNA) is a kind of endogenous non-coding (ncRNA), which produced by the reverse splicing precursor mRNA (pre mRNA). It widely expressed in variety biological cells. Due to special formation mode, circRNA does not have 5' terminal cap and 3' poly (A) tail structure. Compared with linear RNA, more stable exonuclease ribonuclease. In addition, structurally conserved, has sequence tissue-specific. With development high-throughput sequencing bioinformatics technology, circRNAs been found. CircRNA plays an important pathophysiological role occurrence alcoholic liver injury (ALI), hepatic fibrosis (HF), hepatocellular carcinoma (HCC), other diseases. Our group committed research disease diagnosis treatment targets. We review function mechanism ALI, HF HCC, expecting provide new ideas for diagnosis, treatment, prognosis

Language: Английский

Citations

40
CircRNAs and RNA-Binding Proteins Involved in the Pathogenesis of Cancers or Central Nervous System Disorders DOI Creative Commons
Yuka Ikeda, Sae Morikawa,

Moeka Nakashima

et al.

Non-Coding RNA, Journal Year: 2023, Volume and Issue: 9(2), P. 23 - 23

Published: March 31, 2023

Circular RNAs (circRNAs), a newly recognized group of noncoding RNA transcripts, have established widespread attention due to their regulatory role in cell signaling. They are covalently closed that form loop, and typically generated during the splicing precursor RNAs. CircRNAs key post-transcriptional post-translational regulators gene expression programs might influence cellular response and/or function. In particular, circRNAs been considered function as sponges specific miRNA, regulating processes at post-transcription stage. Accumulating evidence has shown aberrant could play pathogenesis several diseases. Notably, circRNAs, microRNAs, RNA-binding proteins, including antiproliferative (APRO) family be indispensable modulators, which strongly linked occurrence addition, attracted general interest for stability, abundance brain, capability cross blood–brain barrier. Here, we present current findings theragnostic potentials With this, aim provide new insights support development novel diagnostic therapeutic strategies these

Language: Английский

Citations

27
Recent advances of exosomal circRNAs in cancer and their potential clinical applications DOI Creative Commons
Qian Yi, Jiaji Yue,

Yang Liu

et al.

Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: July 31, 2023

Abstract Circular RNA (circRNA) is a type of non-coding that forms covalently closed, uninterrupted loop. The expression circRNA differs among cell types and tissues, various circRNAs are aberrantly expressed in variety diseases, including cancer. Aberrantly contribute to disease progression by acting as microRNA sponges, functional protein or novel templates for translation. Recent studies have shown enriched exosomes. Exosomes spherical bilayer vesicles released cells into extracellular spaces mediate intercellular communication delivering cargoes. These cargoes include metabolites, proteins, lipids, molecules. Exosome-mediated cell-cell cell-microenvironment communications influence the carcinogenesis regulating proliferation, angiogenesis, metastasis well immune escape. In this review, we summarize current knowledge about exosomal cancers discuss their specific functions tumorigenesis. Additionally, potential value diagnostic biomarkers applications circRNA-based cancer therapy.

Language: Английский

Citations

25
Comprehensive review for non-coding RNAs: From mechanisms to therapeutic applications DOI
Yanjun Zhang,

Lijuan Zhan,

Xue Jiang

et al.

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 224, P. 116218 - 116218

Published: April 21, 2024

Language: Английский

Citations

15
Non-coding RNAs as therapeutic targets in cancer and its clinical application DOI Creative Commons

Xuejiao Leng,

Mengyuan Zhang,

Yujing Xu

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 14(7), P. 100947 - 100947

Published: Feb. 8, 2024

Cancer genomics has led to the discovery of numerous oncogenes and tumor suppressor genes that play critical roles in cancer development progression. Oncogenes promote cell growth proliferation, whereas inhibit division. The dysregulation these can lead cancer. Recent studies have focused on non-coding RNAs (ncRNAs), including circular RNA (circRNA), long (lncRNA), microRNA (miRNA), as therapeutic targets for In this article, we discuss ncRNAs associated with different types their potential targets. Here, highlight mechanisms action clinical applications treatment. Understanding molecular underlying identifying specific are essential steps towards effective treatments.

Language: Английский

Citations

13
Construction of circRNA–miRNA–mRNA ceRNA regulatory network and screening of diagnostic targets for tuberculosis DOI Creative Commons
Xinyi Pu,

Siyu Sheng,

Yujuan Fu

et al.

Annals of Medicine, Journal Year: 2024, Volume and Issue: 56(1)

Published: Oct. 22, 2024

Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (Mtb), which threatens human health and safety all over the world. Hundreds of thousands people die from TB every year. Timely early diagnosis treatment patients most important measure to control source infection curb epidemic tuberculosis. The existing diagnostic methods have disadvantages poor sensitivity long culture time. Competitive endogenous RNAs (ceRNAs) can regulate expression corresponding target genes competing for same microRNA (miRNA) response elements (MREs) as mRNA. Recent studies found that circRNA has advantages half-life, good stability tissue specificity, be used biomarker predicting, diagnosing treating various diseases, an ideal candidate biomarkers in body fluid biopsy. In this study, transcriptome sequencing was performed on whole blood samples screen out TB-related mirna mRNA differential expression, construct ceRNA regulatory network. Through analysis network, it could competitively bind has-miR-607 induce down-regulation has-miR-607, thereby inhibiting IFNG. hsa_circ_0000566, hsa_circ_0001844, hsa_circ_0005408, hsa_circ_0007587, hsa_circ_0086710, IFNG couble new targets TB. results study not only provide perspective studying potential role network tuberculosis, but also method

Language: Английский

Citations

10
hsa_circ_0001955 Enhances In Vitro Proliferation, Migration, and Invasion of HCC Cells through miR-145-5p/NRAS Axis DOI Creative Commons

Bisha Ding,

Weimin Fan,

Weiyang Lou

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2020, Volume and Issue: 22, P. 445 - 455

Published: Sept. 16, 2020

Increasing circular RNAs (circRNAs) have been reported to act as key players in human malignancies. However, the expression, role, and mechanism of circRNAs HCC are not well elucidated. In this study, some differentially expressed (DECs) between hepatocellular carcinoma (HCC) normal tissues were identified using three circRNA microarrays (Gene Expression Omnibus [GEO]: GSE78520, GSE94508, GSE97332). Twenty-one DECs found be commonly upregulated all datasets. Among 21 DECs, hsa_circ_0001955 ranked top most GEO: GSE97332. Moreover, expression cells was significantly higher than that corresponding controls. Functional experiments revealed knockdown markedly inhibited proliferation, migration, invasion HCC, its overexpression led opposite effects. mainly located cytoplasm, which could directly bind miR-145-5p. miR-145-5p downregulated negatively linked expression. Furthermore, we NRAS a downstream direct target hsa_circ_0001955/miR-145-5p axis HCC. Collectively, our findings demonstrate oncogenic roles hsa_circ_0001955/miR-145-5p/NRAS may represent potential therapeutic for

Language: Английский

Citations

70
CircRNA ciRS-7: a Novel Oncogene in Multiple Cancers DOI Creative Commons
Junwen Chen, Jun Yang, Xiang Fei

et al.

International Journal of Biological Sciences, Journal Year: 2020, Volume and Issue: 17(1), P. 379 - 389

Published: Dec. 16, 2020

circular RNA ciRS-7 (ciRS-7) is a type of endogenous (circRNA) with closed structure.Since Hansen first demonstrated that could serve as microRNA sponge in 2013, researchers have paid increased attention to this circRNA.ciRS-7 plays crucial role regulating transcription, downstream gene expression, and protein production.Moreover, acts an oncogene promotes tumor progression through competitively inhibiting miR-7 various types cancers.ciRS-7 has been identified be closely associated breast cancer, nasopharyngeal carcinoma, lung hepatocellular cervical osteosarcoma, melanoma, colorectal esophageal squamous cell gastric pancreatic laryngeal cholangiocarcinoma.In review, we summarize the biological characteristics, molecular mechanisms, future challenges multiple tumors.

Language: Английский

Citations

59