Mechanistic prediction and validation of Brevilin A Therapeutic effects in Lung Cancer DOI Creative Commons
Ruixue Wang, Cuiyun Gao,

Yu Meng

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 5, 2024

Abstract Background Traditional Chinese medicine (TCM) has been found widespread application in neoplasm treatment, yielding promising therapeutic candidates. Previous studies have revealed the anti-cancer properties of Brevilin A, a naturally occurring sesquiterpene lactone derived from Centipeda minima (L.) A.Br. ( C. ), TCM herb, specifically against lung cancer. However, underlying mechanisms its effects remain elusive. This study employs network pharmacology and experimental analyses to unravel molecular A Methods The Batman-TCM, Swiss Target Prediction, Pharmmapper, SuperPred, BindingDB databases were screened identify targets. Lung cancer-related targets sourced GEO, Genecards, OMIM, TTD, Drugbank databases. Utilizing Cytoscape software, protein-protein interaction (PPI) was established. Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG), set enrichment analysis (GSEA), gene-pathway correlation conducted using R software. To validate results, docking, dynamics simulations, vitro experiments performed. Results We identified 599 A-associated 3864 targets, with 155 overlapping genes considered as candidate for PPI highlighted STAT3, TNF, HIF1A, PTEN, ESR1, MTOR potential GO KEGG 2893 enriched terms 157 pathways, including PI3K-Akt signaling pathway, FoxO HIF-1 pathway. GSEA demonstrated close association between hub Gene-pathway indicated significant associations cellular response hypoxia Molecular docking simulations confirmed A’s PTEN respectively. In A-induced dose- time-dependent cell death A549 cells. Notably, treatment significantly reduced HIF-1α mRNA expression while increasing levels. Conclusions demonstrates that exerts treating cancer through multi-target multi-pathway manner, HIF pathway potentially being involved. These results lay theoretical foundation prospective clinical A.

Language: Английский

Sesquiterpenes and prostate cancer DOI Creative Commons
Kübra Uzun, Cenk Yücel Bilen, Funda Nuray Yalçın

et al.

Phytochemistry Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Language: Английский

Citations

0
Brevilin A Inhibits Prostate Cancer Progression by Decreasing PAX5-Activated SOX4 DOI

Xinxiang Que,

Jianqun Fan,

Desheng Chen

et al.

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: May 14, 2024

Language: Английский

Citations

2
A Review of the Research Applications of Centipeda minima DOI Creative Commons
Jiajun Liu,

Wenying Zheng,

Yifan He

et al.

Molecules, Journal Year: 2023, Volume and Issue: 29(1), P. 108 - 108

Published: Dec. 23, 2023

Centipeda minima is a traditional Chinese medicine with wide applications and diverse pharmacological effects. Scholars have conducted extensive studies on its relevant clinical applications, especially remarkable efficacy in cancer treatment. This paper thoroughly investigates the chemical composition identification, effects, toxicity, along safety of minima, so as to lay foundation for corresponding product development. Furthermore, global scholars research such made significant progress, future development utilization minima’s active ingredients create novel drugs are great significance.

Language: Английский

Citations

4
Pharmacological Activities of Brevilin A: A Mini-Review DOI
Yang Heng, Yan Wu,

Minyi Mo

et al.

Current Pharmacology Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Language: Английский

Citations

1
Mechanistic Prediction and validation of Brevilin A Therapeutic Effects in Lung Cancer DOI Creative Commons
Ruixue Wang, Cuiyun Gao, Meng Yu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 6, 2024

Abstract Background Traditional Chinese medicine (TCM) has found widespread application in neoplasm treatment, yielding promising therapeutic candidates. Previous studies have revealed the anti-cancer properties of Brevilin A, a naturally occurring sesquiterpene lactone derived from Centipeda minima (L.) A.Br. ( C. ), TCM herb, specifically against lung cancer. However, underlying mechanisms its effects remain elusive. This study employs network pharmacology and experimental analyses to unravel molecular A Methods The Batman-TCM, Swiss Target Prediction, Pharmmapper, SuperPred, BindingDB databases were screened identify targets. Lung cancer-related targets sourced GEO, Genecards, OMIM, TTD, Drugbank databases. Utilizing Cytoscape software, protein-protein interaction (PPI) was established. Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG), set enrichment analysis (GSEA), gene-pathway correlation conducted using R software. To validate results, docking, dynamics simulations, vitro experiments performed. Results We identified 599 A-associated 3864 targets, with 155 overlapping genes considered as candidate for PPI highlighted STAT3, TNF, HIF1A, PTEN, ESR1, MTOR potential GO KEGG 2893 enriched terms 157 pathways, including PI3K-Akt signaling pathway, FoxO HIF-1 pathway. GSEA demonstrated close association between hub Gene-pathway indicated significant associations cellular response hypoxia Molecular docking simulations confirmed A's PTEN respectively. In A-induced dose- time-dependent cell death A549 cells. Notably, treatment significantly reduced HIF-1α mRNA expression while increasing levels. Conclusions elucidates intricate responsible on cancer, providing foundation future exploration potential.

Language: Английский

Citations

0
Mechanistic prediction and validation of Brevilin A Therapeutic effects in Lung Cancer DOI Creative Commons
Ruixue Wang, Cuiyun Gao,

Yu Meng

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 5, 2024

Abstract Background Traditional Chinese medicine (TCM) has been found widespread application in neoplasm treatment, yielding promising therapeutic candidates. Previous studies have revealed the anti-cancer properties of Brevilin A, a naturally occurring sesquiterpene lactone derived from Centipeda minima (L.) A.Br. ( C. ), TCM herb, specifically against lung cancer. However, underlying mechanisms its effects remain elusive. This study employs network pharmacology and experimental analyses to unravel molecular A Methods The Batman-TCM, Swiss Target Prediction, Pharmmapper, SuperPred, BindingDB databases were screened identify targets. Lung cancer-related targets sourced GEO, Genecards, OMIM, TTD, Drugbank databases. Utilizing Cytoscape software, protein-protein interaction (PPI) was established. Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG), set enrichment analysis (GSEA), gene-pathway correlation conducted using R software. To validate results, docking, dynamics simulations, vitro experiments performed. Results We identified 599 A-associated 3864 targets, with 155 overlapping genes considered as candidate for PPI highlighted STAT3, TNF, HIF1A, PTEN, ESR1, MTOR potential GO KEGG 2893 enriched terms 157 pathways, including PI3K-Akt signaling pathway, FoxO HIF-1 pathway. GSEA demonstrated close association between hub Gene-pathway indicated significant associations cellular response hypoxia Molecular docking simulations confirmed A’s PTEN respectively. In A-induced dose- time-dependent cell death A549 cells. Notably, treatment significantly reduced HIF-1α mRNA expression while increasing levels. Conclusions demonstrates that exerts treating cancer through multi-target multi-pathway manner, HIF pathway potentially being involved. These results lay theoretical foundation prospective clinical A.

Language: Английский

Citations

0