Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 11, 2023
Abstract
Introduction
:
Cardiac
arrest
(CA)
often
leads
to
severe
neurological
dysfunction
due
inflammation,
mitochondrial
dysfunction,
and
post-cardiopulmonary
resuscitation
(CPR)
damage.
Bone
marrow-derived
mesenchymal
stem
cells
(BMSCs)
show
promise
for
diseases,
but
optimizing
their
therapeutic
potential
neuroregulation
post-CA
remains
unclear.
Methods
We
established
an
in
vitro
co-culture
model
with
BMSCs
post-oxygen-glucose
deprivation
(OGD)
primary
neurons,
confirming
that
hypoxic
preconditioning
enhances
BMSCs'
resistance
neuronal
pyroptosis.
induced
8-minute
CA
through
asphyxia
induction
assessed
preconditioned
bone
(HP-BMSCs)
on
post-resuscitation
oxidative
stress
pyroptosis
using
deficit
scores
(NDS),
brain
tissue
markers,
apoptosis-related
proteins,
area,
damage
markers.
Mechanistic
studies
knocked
down
PFKL
expression
HP-BMSCs
via
si-RNA,
verifying
mechanisms
animals
cells.
Results
Hypoxic
boosted
neuroprotective
effect
against
pyroptosis,
possibly
MAPK
NF-κB
pathway
inhibition.
Consequently,
we
pursued
as
a
neuroprotection
strategy,
RNA
sequencing
suggesting
liver
isoform
of
phosphofructokinase
(PFKL)
regulatory
molecule.
significantly
reduced
stress,
by
CA.
This
manifested
improved
decreased
protein
levels,
enhanced
cell
membrane
structures,
Transfection
PFKL-targeted
si-RNA
into
weakened
protective
effects.
also
confirm
HP-BMSCs'
role
improving
energy
metabolism
following
OGD.
lowered
levels
markers
neurons.
Intracellular
reactive
oxygen
species
(ROS)
dropped,
detected
DCFH-DA
MitoSOX
probes.
Notably,
knocking
reversed
these
Conclusion
In
conclusion,
offer
promising
approach
injury
reducing
mediated
ROS,
potentially
linked
elevated
preconditioning.
Frontiers in Cellular Neuroscience,
Journal Year:
2022,
Volume and Issue:
16
Published: Feb. 3, 2022
Intracerebral
hemorrhage
(ICH)
is
a
devastating
form
of
stroke
with
high
rates
mortality
and
morbidity.
It
induces
cell
death
that
responsible
for
neurological
deficits
postinjury.
There
are
no
therapies
effectively
mitigate
to
treat
ICH.
This
review
aims
summarize
our
knowledge
ICH-induced
focus
on
apoptosis
necrosis.
We
also
discuss
the
involvement
ICH
in
recently
described
modes
including
necroptosis,
pyroptosis,
ferroptosis,
autophagy,
parthanatos.
treatment
strategies
brain
injury
based
particular
pathways
after
Stem Cell Research & Therapy,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: June 7, 2023
Abstract
Mesenchymal
stem/stromal
cells
(MSCs)‐based
therapy
brings
the
reassuring
capability
to
regenerative
medicine
through
their
self‐renewal
and
multilineage
potency.
Also,
they
secret
a
diversity
of
mediators,
which
are
complicated
in
moderation
deregulated
immune
responses,
yielding
angiogenesis
vivo.
Nonetheless,
MSCs
may
lose
biological
performance
after
procurement
prolonged
expansion
vitro.
following
transplantation
migration
target
tissue,
encounter
harsh
milieu
accompanied
by
death
signals
because
lack
proper
tensegrity
structure
between
matrix.
Accordingly,
pre-conditioning
is
strongly
suggested
upgrade
performances
vivo,
leading
more
favored
efficacy
medicine.
Indeed,
ex
vivo
hypoxia,
inflammatory
stimulus,
or
other
factors/conditions
stimulate
survival,
proliferation,
migration,
exosome
secretion,
pro-angiogenic
anti-inflammatory
characteristics
In
this
review,
we
deliver
an
overview
methods
that
considered
strategy
for
improving
therapeutic
organ
failures,
particular,
renal,
heart,
lung,
liver.
Frontiers in Immunology,
Journal Year:
2022,
Volume and Issue:
13
Published: Sept. 5, 2022
Intracerebral
hemorrhage
(ICH)
is
a
highly
harmful
neurological
disorder
with
high
rates
of
mortality,
disability,
and
recurrence.
However,
effective
therapies
are
not
currently
available.
Secondary
immune
injury
cell
death
the
leading
causes
brain
poor
prognosis.
Pyroptosis
recently
discovered
form
programmed
that
differs
from
apoptosis
necrosis
mediated
by
gasdermin
proteins.
caused
multiple
pathways
eventually
pores
in
membrane,
facilitating
release
inflammatory
substances
causing
to
rupture
die.
occurs
neurons,
glial
cells,
endothelial
cells
after
ICH.
Furthermore,
pyroptosis
releases
factors
such
as
interleukin
(IL)-1β
IL-18,
secondary
immune-inflammatory
response
further
damage.
The
NOD-like
receptor
protein
3
(NLRP3)/caspase-1/gasdermin
D
(GSDMD)
pathway
plays
most
critical
role
can
be
inhibited
directly
targeting
NLRP3
or
its
upstream
molecules,
interfering
caspase-1
expression
GSDMD
formation,
thus
significantly
improving
prognosis
present
review
discusses
key
pathological
regulatory
mechanisms
ICH
suggests
possible
intervention
strategies
mitigate
dysfunction
Frontiers in Cellular Neuroscience,
Journal Year:
2022,
Volume and Issue:
16
Published: June 13, 2022
Intracerebral
hemorrhage
(ICH),
a
common
lethal
subtype
of
stroke
accounting
for
nearly
10–15%
the
total
disease
and
affecting
two
million
people
worldwide,
has
high
mortality
disability
rate
and,
thus,
major
socioeconomic
burden.
However,
there
is
no
effective
treatment
available
currently.
The
role
mesenchymal
stem
cells
(MSCs)
in
regenerative
medicine
well
known
owing
to
simplicity
acquisition
from
various
sources,
low
immunogenicity,
adaptation
autogenic
allogeneic
systems,
immunomodulation,
self-recovery
by
secreting
extracellular
vesicles
(EVs),
repair,
antioxidative
stress.
MSC
therapy
provides
an
increasingly
attractive
therapeutic
approach
ICH.
Recently,
functions
MSCs
such
as
neuroprotection,
anti-inflammation,
improvement
synaptic
plasticity
have
been
widely
researched
human
rodent
models
transplantation
proven
improve
ICH-induced
injury,
including
damage
nerve
oligodendrocytes,
activation
microglia
astrocytes,
destruction
blood
vessels.
recovery
neurological
ICH
were
demonstrated
via
mechanisms
neurogenesis,
angiogenesis,
anti-apoptosis,
plasticity.
Here,
we
discuss
pathological
following
MSC-based
unravel
new
cues
future
strategies.
Furthermore,
some
potential
strategies
enhancing
function
also
suggested.
IUBMB Life,
Journal Year:
2023,
Volume and Issue:
75(10), P. 794 - 810
Published: June 6, 2023
Abstract
The
NLRP3
(NOD‐,
LRR‐,
and
pyrin
domain‐containing
protein
3)
inflammasome
is
a
multimeric
complex
that
engaged
in
the
innate
immune
system
plays
vital
role
inflammatory
reactions.
Activation
of
subsequent
release
proinflammatory
cytokines
can
be
triggered
by
microbial
infection
or
cellular
injury.
has
been
implicated
pathogenesis
many
disorders
affecting
central
nervous
(CNS),
ranging
from
stroke,
traumatic
brain
injury,
spinal
cord
injury
to
Alzheimer's
disease,
Parkinson's
epilepsy,
multiple
sclerosis,
depression.
Furthermore,
emerging
evidence
suggested
mesenchymal
stem
cells
(MSCs)
their
exosomes
may
modulate
activation
way
might
promising
for
therapeutic
management
CNS
diseases.
In
present
review,
particular
focus
placed
on
highlighting
discussing
recent
scientific
regarding
regulatory
effects
MSC‐based
therapies
potential
counteract
responses
pyroptotic
cell
death
CNS,
thereby
achieving
neuroprotective
impacts
improvement
behavioral
impairments.
Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
170, P. 116001 - 116001
Published: Dec. 20, 2023
Intervertebral
disc
degeneration
(IVDD)
is
a
main
cause
of
low
back
pain
(LBP),
which
can
lead
to
disability
and
thus
generate
heavy
burden
on
society.
IVDD
characterized
by
decrease
in
nucleus
pulposus
cells
(NPCs)
endogenous
mesenchymal
stem
(MSCs),
degradation
the
extracellular
matrix,
macrophage
infiltration,
blood
vessel
nerve
ingrowth.
To
date,
therapeutic
approaches
regarding
mainly
include
conservative
treatment
surgical
intervention.
However,
both
only
relieve
symptoms
rather
than
stop
or
revert
progression
IVDD,
since
pathogenesis
not
yet
clear.
Pyroptosis,
Caspase
family
dependence
conducted
Gasdermin
family,
newly
discovered
mode
programmed
cell
death.
Pyroptosis
has
been
observed
NPCs,
annulus
fibrosus
(AFCs),
chondrocytes,
MSCs,
macrophages,
vascular
endothelial
neurons
may
contribute
IVDD.
MSCs
are
kind
pluripotent
that
be
found
almost
all
tissues.
have
strong
ability
secrete
vesicles
(EVs),
contain
exosomes,
microvesicles
apoptotic
bodies.
EVs
derived
from
play
an
important
role
pyroptosis
regulation
could
beneficial
for
alleviating
This
review
focuses
clarifying
improve
MSCs.
iScience,
Journal Year:
2024,
Volume and Issue:
27(6), P. 109865 - 109865
Published: April 30, 2024
Previous
studies
have
indicated
the
neuroprotective
effect
of
olfactory
mucosa
mesenchymal
stem
cells
(OM-MSCs)
on
brain
injury.
Intracerebral
hemorrhage
(ICH)
models
were
established
in
rats
by
injecting
autologous
blood.
SENP1
expression
was
enhanced
neurons
but
decreased
astrocytes
compared
to
that
OM-MSCs.
Overexpression
promoted
proliferation
and
neuronal
differentiation,
while
inhibiting
astrocytic
differentiation
Conversely,
its
knockdown
had
opposite
effect.
Moreover,
OM-MSCs
reduced
neurological
dysfunction
after
ICH,
could
be
further
overexpression.
In
addition,
mitophagy,
which
might
related
SENP1-mediated
OPTN
deSUMOylation.
Furthermore,
through
mitophagy
mediated
OPTN.
Similar
SENP1,
transfection
remission
OM-MSC
ICH
rats.
OPTN-mediated
improve
deficits
World Journal of Stem Cells,
Journal Year:
2024,
Volume and Issue:
16(6), P. 623 - 640
Published: June 24, 2024
The
stem
cell
pre-treatment
approaches
at
cellular
and
sub-cellular
levels
encompass
physical
manipulation
of
cells
to
growth
factor
treatment,
genetic
manipulation,
chemical
pharmacological
each
strategy
having
advantages
limitations.
Most
these
protocols
are
non-combinative.
This
editorial
is
a
continuum
Li
