Expression pattern, tumor immune landscape, and prognostic value of N7‑methylguanosine regulators in bladder urothelial carcinoma DOI Open Access
Chi Zhang, Jiangnan Xia, Simiao Zhang

et al.

Oncology Letters, Journal Year: 2023, Volume and Issue: 25(4)

Published: March 10, 2023

N7‑Methylguanosine (m7G) modification is important in post‑transcriptional regulation. dysregulation of m7G RNA has been reported to be markedly associated with cancer. However, its importance bladder urothelial carcinoma (BLCA) remains poorly characterized. The present study systematically analyzed mRNA gene expression data and clinical information from Cancer Genome Atlas further constructed robust risk signatures for the four regulators (nudix hydrolase 11, gem nuclear organelle‑associated protein 5, eukaryotic translation initiation factor 3 subunit D cytoplasmic FMR1 interacting 1). differential cell function m7G‑related genes cancer cells were verified by reverse transcription‑quantitative PCR, Cell Counting Kit‑8 colony formation assays. four‑gene‑based model could accurately predict prognosis BLCA. Nomogram‑based decisions had a higher net benefit compared that individual predictors. Through immune infiltration analysis, it was found affected patients Finally, identified potential therapeutics differ between high low‑risk groups based on genes. In summary, current findings revealed an essential role BLCA, developed as promising prognostic markers

Language: Английский

Research progress of N1-methyladenosine RNA modification in cancer DOI Creative Commons
Yafeng Liu, Shujun Zhang, Xiaohui Gao

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 30, 2024

Abstract N1-methyladenosine (m1A) is a post-transcriptionally modified RNA molecule that plays pivotal role in the regulation of various biological functions and activities. Especially cancer cell invasion, proliferation cycle regulation. Over recent years, there has been burgeoning interest investigating m1A modification RNA. Most studies have focused on enrichment areas different regions. This review provides comprehensive overview methodologies employed for detection modification. Furthermore, this delves into key players modification, known as “writers,” “erasers,” “readers.” by methyltransferases, or writers, such TRMT6, TRMT61A, TRMT61B, TRMT10C, NML, and, removed demethylases, erasers, including FTO ALKBH1, ALKBH3. It recognized m1A-binding proteins YTHDF1, TYHDF2, TYHDF3, TYHDC1, also “readers”. Additionally, we explore intricate relationship between its regulators their implications development progression specific types cancer, discuss how can potentially facilitate discovery novel approaches diagnosis, treatment, prognosis. Our summary methylated adenosine methods regulatory mechanisms cancers useful insights

Language: Английский

Citations

17
Pharmacodynamic components and mechanisms of ginger (Zingiber officinale) in the prevention and treatment of colorectal cancer DOI

Si-Rui Xiang,

Jian Qin, Chen Wu

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 324, P. 117733 - 117733

Published: Jan. 12, 2024

Language: Английский

Citations

10
N7-methylguanosine modification in cancers: from mechanisms to therapeutic potential DOI Creative Commons
Qihui Wu, Xiaodan Fu,

Guoqian Liu

et al.

Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 29, 2025

N7-methylguanosine (m7G) is an important RNA modification involved in epigenetic regulation that commonly observed both prokaryotic and eukaryotic organisms. Their influence on the synthesis processing of messenger RNA, ribosomal transfer allows m7G modifications to affect diverse cellular, physiological, pathological processes. are pivotal human diseases, particularly cancer progression. On one hand, modification-associated modulate tumour progression malignant biological characteristics, including sustained proliferation signalling, resistance cell death, activation invasion metastasis, reprogramming energy metabolism, genome instability, immune evasion. This suggests they may be novel therapeutic targets for treatment. other aberrant expression molecules linked clinicopathological staging, lymph node unfavourable prognoses patients with cancer, indicating their potential as biomarkers. review consolidates discovery, identification, detection methodologies, functional roles modification, analysing mechanisms by which contribute development, exploring clinical applications diagnostics therapy, thereby providing innovative strategies identification targeted

Language: Английский

Citations

1
The m6A demethylases FTO and ALKBH5 aggravate the malignant progression of nasopharyngeal carcinoma by coregulating ARHGAP35 DOI Creative Commons
Zhiyuan Yang, Siyu Zhang,

Jiayan Xiong

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 23, 2024

Abstract N 6 -methyladenosine (m A) is an RNA modification that can be removed by demethylases [fat mass and obesity-associated protein (FTO) AlkB homolog 5 (ALKBH5)], which regulate gene expression cell function. We show m A levels m6A demethylase are altered in nasopharyngeal carcinoma (NPC) tissues vs. normal tissues. High FTO ALKBH5 predict a poor prognosis NPC patients. Silencing inhibited the malignant behavior of patient-derived cells short time. However, as time progressed, inhibitory effect or was weakened, cosilencing maintained better effect. Combined transcriptome A-seq analysis revealed downstream target jointly regulated NPC, ARHGAP35 chosen to do further study. The synergistic silencing increased methylation level on mRNA CDS new transcription factor (ARHGAP35) positively coding capacity stability ARHGAP35, thus leading inhibition phenotype tumor cells. Our study growth metastasis stably through ALKBH5, play positive role treatment regulating transcript increasing its level.

Language: Английский

Citations

6
Pharmacology of Epitranscriptomic Modifications: Decoding the Therapeutic Potential of RNA Modifications in Drug Resistance DOI
Abdullah Alkhammash

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 994, P. 177397 - 177397

Published: Feb. 18, 2025

Language: Английский

Citations

0
Pseudogene: Relevant or Irrelevant? DOI Creative Commons
Yang-Hsiang Lin,

Chau‐Ting Yeh,

Cheng‐Yi Chen

et al.

Biomedical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 100790 - 100790

Published: Sept. 1, 2024

Language: Английский

Citations

2
Revisiting epigenetic regulation in cancer: Evolving trends and translational implications DOI
Mohammad Afsar Khan, Dhruv Mishra, Ranjan Kumar

et al.

International review of cell and molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 24

Published: Oct. 4, 2024

Language: Английский

Citations

2
Identification and analysis of RNA-5-methylcytosine-related key genes in osteoarthritis DOI Creative Commons
Yang Yu, Shitao Lu, Xiaoming Liu

et al.

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: Sept. 12, 2023

5-methylcytosine (m5C) modification is widely associated with many biological and pathological processes. However, knowledge of m5C in osteoarthritis (OA) remains lacking. Thus, our study aimed to identify common features OA.In the present study, we identified 1395 differentially methylated genes (DMGs) 1673 expressed (DEGs) using RNA immunoprecipitation next-generation sequencing (MeRIP-seq) RNA-sequencing. A co-expression analysis DMGs DEGs showed that expression 133 was significantly affected by methylation. protein-protein interaction network constructed STRING database, cytoHubba plug-in Cytoscape used hub were screen out 11 genes, including MMP14, VTN, COL15A1, COL6A2, SPARC, COL5A1, COL6A3, COL6A1, COL8A2, ADAMTS2 COL7A1. The Pathway enrichment ClueGO CluePedia plugins enriched collagen degradation extracellular matrix degradation.Our indicated might play an important role OA pathogenesis, provides worthwhile insight into identifying m5C-related therapeutic targets OA.

Language: Английский

Citations

4
The role of RNA modifications in hepatocellular carcinoma: functional mechanism and potential applications DOI Creative Commons

Jin‐Xiu Liu,

Xiaoping Zhang, Wenhua Xu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 20, 2024

Hepatocellular carcinoma (HCC) is a highly aggressive cancer with poor prognosis. The molecular mechanisms underlying its development remain unclear. Recent studies have highlighted the crucial role of RNA modifications in HCC progression, which indicates their potential as therapeutic targets and biomarkers for managing HCC. In this review, we discuss functional through review summary relevant literature, to explore agents diagnostic prognostic This that specific modification pathways, such N6-methyladenosine, 5-methylcytosine, N7-methylguanosine, N1-methyladenosine, are erroneously regulated involved proliferation, autophagy, innate immunity, invasion, metastasis, immune cell infiltration, drug resistance These findings provide new perspective understanding HCC, well diagnosis treatment by targeting RNA-modifying enzymes or recognition proteins. More than ten regulators showed use diagnosis, prognosis decision utility Their application value necessitates extensive multi-center sample validation future. A growing number modifier inhibitors being developed, but lack preclinical experiments clinical poses significant obstacle, further research needed evaluate treatment. conclusion, provides an in-depth complex interplay between while emphasizing promising

Language: Английский

Citations

1
Deciphering the Divergent Gene Expression Landscapes of m6A/m5C/m1A Methylation Regulators in Hepatocellular Carcinoma Through Single-Cell and Bulk RNA Transcriptomic Analysis DOI Creative Commons

Hang-Tsung Liu,

Cheng‐Shyuan Rau,

Yueh‐Wei Liu

et al.

Journal of Hepatocellular Carcinoma, Journal Year: 2023, Volume and Issue: Volume 10, P. 2383 - 2395

Published: Dec. 1, 2023

Introduction: RNA modifications mediated by the m6A, m1A, and m5C regulatory genes are crucial for progression of malignancy.This study aimed to explore expression regulator m6A/m5C/m1A methylation at single-cell level validate their in cancerous adjacent para-cancerous liver tissues adult patients with HCC who underwent tumor resection.Methods: The bulk sequencing from Cancer Genome Atlas (TCGA) database (scRNA-seq) data obtained Gene Expression Omnibus (GEO) were used identify dysregulated hepatocellular carcinoma (HCC).A real-time polymerase chain reaction (real-time PCR) was measure collected human compared tissues.Immune cell infiltration these significantly expressed methylation-related evaluated using Timer2.0.Results: A discrepancy gene observed between scRNA-seq.The clustered heatmap scRNA-seq-identified TCGA cohort revealed heterogeneous regulators within cancer, whereas more homogeneous.The PCR validated significant overexpression DNMT1, NSUN5, TRMT6, IGF2BP1, IGFBP3, which identified scRNA-seq, IGFBP2, sequencing.These mainly correlated natural killer cells.Discussion: This suggests that cellular diversity inside tumors contributes traditional scRNA-seq.This five will be focus further studies regarding function HCC.

Language: Английский

Citations

3