Nigella sativa L. and COVID-19: A Glance at The Anti-COVID-19 Chemical Constituents, Clinical Trials, Inventions, and Patent Literature

Molecules, Journal Year: 2022, Volume and Issue: 27(9), P. 2750 - 2750

Published: April 25, 2022

COVID-19 has had an impact on human quality of life and economics. Scientists have been identifying remedies for its prevention treatment from all possible sources, including plants.

Language: Английский

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Natural Bioactive Compounds Targeting NADPH Oxidase Pathway in Cardiovascular Diseases

Molecules, Journal Year: 2023, Volume and Issue: 28(3), P. 1047 - 1047

Published: Jan. 20, 2023

Cardiovascular disease (CVD) is the leading cause of death worldwide, in both developed and developing countries. According to WHO report, morbidity mortality caused by CVD will continue rise with estimation going up 22.2 million 2030. NADPH oxidase (NOX)-derived reactive oxygen species (ROS) production induces endothelial nitric oxide synthase (eNOS) uncoupling mitochondrial dysfunction, resulting sustained oxidative stress development cardiovascular diseases. Seven distinct members family have been identified which four (namely, NOX1, 2, 4 5) may functions. Currently, treatment management plan for patients CVDs mainly depends on drugs. However, prolonged use prescribed drugs adverse drug reactions. Therefore, it crucial find alternative options lesser effects. Natural products gaining interest as complementary therapy over past decade due their wide range medicinal properties, including antioxidants. These might be potent active ingredients, such flavonoid phenolic compounds. Numerous natural compounds demonstrated advantageous effects via cascade. This review highlights potential targeting NOX-derived ROS generation treating CVDs. Emphasis put activation oxidases, upstream or downstream signalling events.

Language: Английский

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Identification and Functional Analysis of PANoptosis‐Associated Genes in the Progression From Sepsis to ARDS

Immunity Inflammation and Disease, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 1, 2025

ABSTRACT Background Sepsis and acute respiratory distress syndrome (ARDS) are common inflammatory conditions in intensive care, with ARDS significantly increasing mortality septic patients. PANoptosis, a newly discovered form of programmed cell death involving multiple pathways, plays critical role diseases. This study aims to elucidate the PANoptosis‐related genes (PRGs) their involvement progression sepsis ARDS. Methods analyzed differentially expressed (DEGs) associated PRGs explore immune disorders from A diagnostic prediction model was constructed based on key identified through bioinformatics analysis. Functional enrichment analyses were conducted determine pathway involvement, correlations cells assessed. Mendelian randomization analysis applied investigate potential causal links between specific Immunohistochemical used evaluate PRG expression lung tissue. Results The effectively distinguished patients those sepsis. NDRG1 elevated ARDS, while DDX3X, PTPRC, TNFSF8 downregulated. showed positive correlation activated dendritic cells, whereas positively neutrophils negatively correlated CD56bright NK cells. highlighted spliceosome function, MAPK signaling, endocytosis, antigen processing pathways as these PRGs. suggested link immunohistochemical revealed its predominant near vascular walls. In mouse sepsis, suppression alleviated injury. Conclusion PANoptosis may contribute dysregulation sepsis‐associated is therapeutic target, offering new avenues for mitigating improving patient outcomes.

Language: Английский

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Potential role of thymoquinone to treat gastrointestinal cancers: insights into its molecular mechanisms

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Language: Английский

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Novel Thymoquinone Nanoparticles Using Poly(ester amide) Based on L-Arginine-Targeting Pulmonary Drug Delivery

Polymers, Journal Year: 2022, Volume and Issue: 14(6), P. 1082 - 1082

Published: March 8, 2022

Thymoquinone (TQ), the main active constituent of Nigella sativa, has demonstrated broad-spectrum antimicrobial, antioxidant, and anti-inflammatory effects, which suggest its potential use in secondary infections caused by COVID-19. However, clinical deployment been hindered due to limited aqueous solubility poor bioavailability. Therefore, a targeted delivery system lungs using nanotechnology is needed overcome limitations encountered with TQ. In this project, novel TQ-loaded poly(ester amide) based on L-arginine nanoparticles was prepared interfacial polycondensation method for dry powder inhaler targeting TQ lungs. The were characterized FTIR NMR confirm structure. Transmission electron microscopy Zetasizer results confirmed particle diameter 52 nm. high-dose formulation showed entrapment efficiency loading capacity values be 99.77% 35.56%, respectively. An XRD study proved that did not change crystallinity, further DSC study. Optimized evaluated their vitro aerodynamic performance, an effective 22.7-23.7% nominal dose into lower parts high drug-targeting demonstrates significant role application COVID-19 other respiratory conditions.

Language: Английский

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Box-Behnken Design (BBD) Application for Optimization of Chromatographic Conditions in RP-HPLC Method Development for the Estimation of Thymoquinone in Nigella sativa Seed Powder

Processes, Journal Year: 2022, Volume and Issue: 10(6), P. 1082 - 1082

Published: May 29, 2022

Thymoquinone (THY) is a bioactive compound present in the seed powder of Nigella sativa (NS). This research aims to precisely and accurately estimate THY using high-performance liquid chromatography (HPLC) with Quality by Design (QbD) application. Box-Behnken design (BBD) was employed optimize chromatographic conditions for HPLC method development, taking mobile phase flow rate, pH buffer, λmax as independent variables retention time tailing factor measured responses. The composition methanol: acetonitrile: buffer (2.2 mM ammonium formate) at ratio 35:50:15 v/v/v on Symmetry® C18 (5 μm, 3.9 × 150 mm) column. In isocratic mode, it had rate 0.9 mL min−1 eluted analyte detected 249 nm. Validation parameters followed International Council Harmonization (ICH) guidelines new method. linear over range 6.25–100 µg mL−1 coefficient determination (r2) 0.9957. limit detection (LOD) quantification (LOQ) were 2.05 6.25 mL−1, respectively. %RSD system suitability 1.42% 0.695%. addition, developed precise, accurate, robust according ICH criteria. simple, quick, robust, could be used routine analysis different kinds formulations.

Language: Английский

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Pharmacologic effects approach of essential oils and their components on respiratory diseases

Journal of Ethnopharmacology, Journal Year: 2022, Volume and Issue: 304, P. 115962 - 115962

Published: Dec. 15, 2022

Language: Английский

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Thymoquinone Alterations of the Apoptotic Gene Expressions and Cell Cycle Arrest in Genetically Distinct Triple-Negative Breast Cancer Cells

Nutrients, Journal Year: 2022, Volume and Issue: 14(10), P. 2120 - 2120

Published: May 19, 2022

Breast cancer (BC) is the most common in women worldwide, and it one of leading causes death women. triple-negative breast Cancer (TNBC), a subtype BC, typically associated with highest pathogenic grade incidence premenopausal young African American (AA) Chemotherapy, treatment for TNBC today, can lead to acquired resistance ineffective treatment. Therefore, novel therapeutic approaches are needed combat medication ineffectiveness patients. Thymoquinone (TQ) shown have cytotoxic effect on human cells vitro. However, TQ’s mode action precise mechanism disease vitro not been adequately investigated. effects genetically different MDA-MB-468 MDA-MB-231 cell lines were assessed. The data obtained show that TQ displayed time- concentration-dependent manner after 24 h, IC50 values 25.37 µM 27.39 µM, respectively. Moreover, scratched wound-healing assay poor wound closure, inhibiting invasion migration via cycle blocking h. arrested phase cells. three stages significantly affected at 15 20 G0/G1 S phases, as well all concentrations G2/M phases. In cells, there was significant decrease phases substantial increase contrast, showed only during two (S G2/M), apoptotic gene profiles indicated upregulated genes including caspases, GADD45A, TP53, DFFA, DIABLO, BNIP3, TRAF2/3, TNFRSF10A. 16 upregulated, TNFRSF10A, TNF, TNFRSF11B, FADD TNFRSF10B, CASP2, TRAF2, which important pathway andsuppress expression anti-apoptotic gene, BIRC5, Compared elevated levels TNF their receptor proteins may contribute increased sensitivity TQ-induced apoptosis. It concluded from this study targets differently. Additionally, due aggressive nature lack specific therapies chemoresistant TNBC, our findings related identified profile point potential agent therapy.

Language: Английский

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Thymoquinone enhances the antioxidant and anticancer activity of Lebanese propolis

World Journal of Clinical Oncology, Journal Year: 2023, Volume and Issue: 14(5), P. 203 - 214

Published: May 19, 2023

Reactive oxygen species (ROS) are produced by multiple cellular processes and maintained at optimal levels in normal cells endogenous antioxidants. In recent years, the search for potential exogenous antioxidants from dietary sources has gained considerable attention to eliminate excess ROS that is associated with oxidative stress related diseases including cancer. Propolis, a resinous honeybee product, been shown have protective effects against anticancer several types of neoplasms.

Language: Английский

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Therapeutic Potential of Thymoquinone and Its Nanoformulations in Pulmonary Injury: A Comprehensive Review

International Journal of Nanomedicine, Journal Year: 2021, Volume and Issue: Volume 16, P. 5117 - 5131

Published: July 1, 2021

Abstract: As a crucial organ, the lung is exposed to various harmful agents that may induce inflammation and oxidative stress, which cause chronic or acute injury. Nigella sativa , also known as black seed, has been widely used treat diseases one of most extensively researched medicinal plants. Thymoquinone (TQ) main component seed volatile oil proven have antioxidant, anti-inflammatory, antineoplastic properties. The potential therapeutic properties TQ against pulmonary disorders studied in both vitro vivo studies. Furthermore, application nanotechnology increase drug solubility, cellular absorption, release (sustained control), delivery tissue target sites. result, fabricating nanoparticles (NPs) approach variety diseases. In this current review, we summarize recent findings on efficacy its nanotypes caused by immunocompromised conditions such cancer, diabetes, gastric ulcers, other neurodegenerative It concluded with antiasthma, antitumor activity be safely applied disorders. However, more research required before can pharmaceutical preparations human Keywords: anticancer activity, antimicrobial bioavailability, delivery, molecular potential, disease, nanoparticle, thymoquinone

Language: Английский

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