Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug DOI Creative Commons
Foteini Gkartziou, Maria Plota,

Charikleia Kypraiou

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(4), P. 459 - 459

Published: March 26, 2024

The purpose of the present study was to investigate anti-staphylococcal activity liposomal daptomycin against four biofilm-producing S. aureus and epidermidis clinical strains, three which are methicillin-resistant. Neutral negatively charged daptomycin-loaded liposomes were prepared using methods, namely, thin-film hydration (TFH), a dehydration–rehydration vesicle (DRV) method, microfluidic mixing (MM); moreover, they characterized for drug encapsulation (EE%), size distribution, zeta-potential, stability, release, integrity. Interestingly, whilst loading in THF DRV nanosized (by extrusion) vesicles around 30–35, very low (~4%) possible MM vesicles, requiring further explanatory investigations. Liposomal protected from degradation preserved its bioactivity. Biofilm mass (crystal violet, CV), biofilm viability (MTT), growth curve (GC) assays evaluated antimicrobial neutral daptomycin-liposomes towards planktonic bacteria biofilms. exhibited dramatically enhanced inhibition bacterial (compared free drug) all species studied, while totally inactive. prevention treatment studies revealed high antibiofilm daptomycin. more active negative charge ones treating established Planktonic as well matured biofilms daptomycin-susceptible, methicillin-resistant Staphylococcus (MRSA) (MRSE) strains almost completely eradicated by liposomal-daptomycin, indicating need their exploration therapeutics.

Language: Английский

Unraveling the secrets: Evolution of resistance mediated by membrane proteins DOI Creative Commons
Xue Yang, Min Li,

Zi-Chang Jia

et al.

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 77, P. 101140 - 101140

Published: Aug. 16, 2024

Membrane protein-mediated resistance is a multidisciplinary challenge that spans fields such as medicine, agriculture, and environmental science. Understanding its complexity devising innovative strategies are crucial for treating diseases like cancer managing resistant pests in agriculture. This paper explores the dual nature of mechanisms across different organisms: On one hand, animals, bacteria, fungi, plants, insects exhibit convergent evolution, leading to development similar mechanisms. other influenced by diverse pressures structural differences among organisms, they also demonstrate divergent characteristics. prevalent insects, reflecting their shared survival evolved through evolution address challenges. However, variations ecological environments biological characteristics result differing responses resistance. Therefore, examining these not only enhances our understanding adaptive but provides theoretical support insights addressing drug advancing pharmaceutical development.

Language: Английский

Citations

10
Molecular and genetic analysis of methicillin-resistant Staphylococcus aureus (MRSA) in a tertiary care hospital in Saudi Arabia DOI
Mohd Saleem, Irfan Ahmad, Alaa Salem

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Language: Английский

Citations

1
Synthesis of tetra-substituted thiophene derivatives as potential Hits combating antibiotic resistant bacteria ESKAPE DOI
Heba K. Abd El-Mawgoud, Asmaa M. AboulMagd, Ahmed Shaker

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 155, P. 108101 - 108101

Published: Jan. 8, 2025

Language: Английский

Citations

1
Microwave-Assisted Synthesized ZnO@APTES Quantum Dots Exhibits Potent Antibacterial Efficacy Against Methicillin-Resistant Staphylococcus aureus Without Inducing Resistance DOI Creative Commons

Fangyuan Du,

Jingqi Niu,

Yu Hong

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 523 - 540

Published: Jan. 1, 2025

Antibiotic resistance of many bacteria, including Methicillin-resistant Staphylococcus aureus (MRSA), has become a major threat to global health. Zinc Oxide Quantum dots (ZnO-QDs) show good antibacterial activity, but most them are insoluble in water, limiting their application range, and there is lack research on drug inducement. The water-soluble zinc oxide quantum modified by APTES (ZnO@APTES QDs) were prepared microwave assisted synthesis. Then ZnO@APTES QDs characterized through various methods. After confirmation synthesized QDs, its bactericidal effect MRSA was detected vitro vivo experiments, mechanism action analyzed. Characterization analysis revealed that the have particle size 5 nm. minimum inhibitory concentrations (MIC) determined be 64 µg mL-1 for Escherichia coli (E. coli) 32 MRSA. showed significant inhibition biofilm formation effectively disrupted mature biofilms. Notably, did not induce tolerance or even after 30 days repeated exposure, whereas antibiotics led rise bacterial MIC within 3 60-fold increase days. Mechanistic indicated positively charged interact with surfaces, altering membrane fluidity. Once inside generate reactive oxygen species (ROS), causing DNA damage cell death. Moreover, possessed biocompatibility demonstrated therapeutic efficacy against drug-resistant infections both macrophage mouse wound infection models. In summary, we highly effective shows strong other bacteria. holds potential development as new treatment agent combating antibiotic-resistant infections.

Language: Английский

Citations

1
Current strategies against multidrug-resistant Staphylococcus aureus and advances toward future therapy DOI Creative Commons
Saurabh Kumar, Richa Prasad Mahato,

Sanjay Ch

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100281 - 100281

Published: Feb. 1, 2025

Language: Английский

Citations

1
Public Patterns and Determinants of Antibiotic Self-Medication and Antibiotic Knowledge in Southern Jordan DOI Creative Commons

Alaa Al-Tarawneh,

Tasneem Ali,

Ghaith M. Al‐Taani

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(1), P. 98 - 98

Published: Jan. 19, 2024

Antibiotic self-medication, which refers to acquisition and using antibiotics treat infections based on personal experience and/or without a doctor’s advice or prescription, is significant public health issue jeopardizing patient outcomes. The purpose of the present cross-sectional online survey was assess frequency self-medication among general in various geographical locations southern Jordan, as well examine determinants self-medication. distributed through several social media networks over period November–December 2022, included demographic information items related use abuse antibiotics, sources about duration assessment knowledge appropriate antibiotic use. Inferential analysis, such Chi-Square test logistic regression, were adopted associations between different variables with A total 984 respondents enrolled study. Of these, 752 had been during last year. However, self-medicating cases 413 752. main source utilization participants pharmacists. commonly (36.0%) tended until symptoms disappeared. Nearly half reported usually taking for treating runny nose (rhinorrhea). regression analysis indicated that significantly associated female gender (p-value < 0.001), low educational level = 0.014), rural living location 0.003), no insurance 0.001) occupation 0.005). Meanwhile age relationship Finally, results revealed poor understanding key usage, inevitably influences practice. It crucial come up programs governmental policies suppress widespread it will affect future generations Jordanian citizens.

Language: Английский

Citations

6
Antibacterial Interactions of Ethanol-Dispersed Multiwalled Carbon Nanotubes with Staphylococcus aureus and Pseudomonas aeruginosa DOI Creative Commons
Mihaela Asaftei, Massimiliano Lucidi, Stefan R. Anton

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(31), P. 33751 - 33764

Published: July 9, 2024

Infectious diseases are acknowledged as one of the leading causes death worldwide. Statistics show that annual toll caused by bacterial infections has reached 14 million, most which drug-resistant strains. Bacterial antibiotic resistance is currently regarded a compelling problem with dire consequences, motivates urgent identification alternative ways fighting bacteria. Various types nanomaterials have been reported to date efficient antibacterial solutions. Among these, carbon-based nanomaterials, such carbon nanodots, graphene oxide, and nanotubes (CNTs), shown be effective in killing wide panel pathogenic With this study, we aim provide additional insights into topic research investigating activity specific type multiwalled CNTs, diameters from 50 150 nm, against two representative opportunistic pathogens, i.e., Gram-positive bacterium

Language: Английский

Citations

4
Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications DOI Creative Commons

Mina Golban,

Javad Charostad,

Hossein Kazemian

et al.

Infectious Diseases and Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 16, 2024

Antimicrobial resistance is a significant global public health issue, and the dissemination of antibiotic in Gram-positive bacterial pathogens has significantly increased morbidity, mortality rates, healthcare costs. Among them, Staphylococcus, especially methicillin-resistant Staphylococcus aureus (MRSA), causes wide range diseases due to its diverse pathogenic factors infection strategies. These bacteria also present issues veterinary medicine food safety. Effectively managing staphylococci-related problems necessitates concerted effort implement preventive measures, rapidly detect pathogen, develop new safe antimicrobial therapies. In recent years, there been growing interest using endolysins combat infections. enzymes, which are referred as lysins, unique class hydrolytic enzymes synthesized by double-stranded DNA bacteriophages. They possess glycosidase, lytic transglycosylase, amidase, endopeptidase activities, effectively destroying peptidoglycan layer resulting lysis. This property makes powerful agents, particularly against organisms with more accessible layers. Therefore, considering potential benefits compared conventional antibiotics, we have endeavored gather review characteristics uses derived from staphylococcal bacteriophages, well their antibacterial effectiveness spp. based on conducted experiments trials.

Language: Английский

Citations

4
Unveiling novel antimicrobial peptides from the ruminant gastrointestinal microbiomes: A deep learning-driven approach yields an anti-MRSA candidate DOI Creative Commons
Hong Shen, Yanru Li,

Qingjie Pi

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Antimicrobial peptides (AMPs) present a promising avenue to combat the growing threat of antibiotic resistance. The ruminant gastrointestinal microbiome serves as unique ecosystem that offers untapped potential for AMP discovery.

Language: Английский

Citations

0
Prevalence and Molecular Characterization of Carbapenemase-Producing Multidrug-Resistant Bacteria in Diabetic Foot Ulcer Infections DOI Creative Commons
Mohd Saleem, Soha Abdallah Moursi, Tahani Altamimi

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(2), P. 141 - 141

Published: Jan. 9, 2025

Background: Diabetic foot ulcers (DFUs) represent severe complications in diabetic patients, often leading to chronic infections and potentially resulting nontraumatic lower-limb amputations. The increasing incidence of multidrug-resistant (MDR) bacteria DFUs complicates treatment strategies worsens patient prognosis. Among these pathogens, carbapenemase-producing pathogens have emerged as particularly concerning owing their resistance β-lactam antibiotics, including carbapenems. Methods: This study evaluated the prevalence MDR bacteria, specifically DFU infections. A total 200 clinical isolates from patients were analyzed via phenotypic assays, modified Hodge test (MHT) Carba NP test, alongside molecular techniques detect carbapenemase-encoding genes (blaKPC, blaNDM, blaVIM, blaIMP, blaOXA-48). Results: isolates, 51.7% confirmed be carbapenemase producers. key identified included Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli. most commonly detected blaKPC (27.6%) blaNDM (24.1%). Carbapenemase-producing presented high whereas non-carbapenemase-producing through mechanisms such porin loss efflux pumps. Conclusions: findings this highlight significant burden infections, organisms, DFUs. strongly associated with critical parameters, pyrexia (p = 0.017), recent antibiotic use 0.003), severity Notably, need for minor amputations was much higher cases < 0.001), major 0.001). also polymicrobial Furthermore, Wagner ulcer grade ≥II more common 0.002). These results emphasize urgent enhanced microbiological surveillance development tailored antimicrobial combat effectively. Given carbapenem resistance, there is an immediate explore novel therapeutic options improve outcomes

Language: Английский

Citations

0