One-pot hydrothermal synthesis of Si-doped carbon quantum dots with up-conversion fluorescence as fluorescent probes for dual-readout detection of berberine hydrochloride

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2022, Volume and Issue: 275, P. 121139 - 121139

Published: March 11, 2022

Language: Английский

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Minocycline-Loaded Poly(α-Lipoic Acid)–Methylprednisolone Prodrug Nanoparticles for the Combined Anti-Inflammatory Treatment of Spinal Cord Injury

International Journal of Nanomedicine, Journal Year: 2022, Volume and Issue: Volume 17, P. 91 - 104

Published: Jan. 1, 2022

Traumatic spinal cord injury (TSCI) induces a powerful inflammatory response that can significantly exacerbate the extent and severity of neural damage (termed as "secondary injury"). Thus, suppression inflammation is crucial for reducing neurological dysfunction following TSCI. However, conventional anti-inflammatory drugs show limited efficacy because poor penetration release kinetics at site. This study describes design, synthesis, kinetics, biosafety, preclinical minocycline (MC)-loaded poly(α-lipoic acid)-methylprednisolone (PαLA-MP) prodrug nanoparticles (NPs) combined treatment TSCI.NPs were produced by conjugating MP to PαLA then loading MC. The NP structure was confirmed through 1H nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy, ultraviolet-visible absorption gel permeation chromatography, dynamic light scattering, transmission electron microscopy. Drug-loading content measured using high-performance liquid chromatography (HPLC) or NMR HPLC. Biosafety examined MTT assay, cell efficiency confocal microscopy, flow cytometry Cyanine5 (Cy5)-labeled MC-PαLA-MP NPs, effects on injury-induced pro-inflammatory cytokine enzyme-linked immunosorbent assays immunofluorescence, measuring motor recovery in rat model TSCI.The NPs exhibited high biocompatibility released 81% MC 54% within 24 h under TSCI-like conditions, effectively 40% both cultures injured tissues. Systemic injection increased Basso, Beattie, Bresnahan score TSCI rats from 2.33 ± 0.52 8.83 1.83 8 weeks, providing effective neuroprotection enhanced exercise rats.The mitigate secondary preserve function experimental TSCI, which suggests their potential clinical application.

Language: Английский

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Nanomedicine innovations in spinal cord injury management: Bridging the gap

Environmental Research, Journal Year: 2023, Volume and Issue: 235, P. 116563 - 116563

Published: July 7, 2023

Language: Английский

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Self‐Assembly of Selenium‐Doped Carbon Quantum Dots as Antioxidants for Hepatic Ischemia‐Reperfusion Injury Management

Small, Journal Year: 2023, Volume and Issue: 19(30)

Published: April 6, 2023

Abstract Hepatic ischemia‐reperfusion injury (HIRI) is a critical complication after liver surgery that negatively affects surgical outcomes of patients with the end‐stage liver‐related disease. Reactive oxygen species (ROS) are responsible for development and eventually lead to hepatic dysfunction. Selenium‐doped carbon quantum dots (Se‐CQDs) an excellent redox‐responsive property can effectively scavenge ROS protect cells from oxidation. However, accumulation Se‐CQDs in extremely low. To address this concern, fabrication Se‐CQDs‐lecithin nanoparticles (Se‐LEC NPs) developed through self‐assembly mainly driven by noncovalent interactions. Lecithin acting as building block also makes pivotal contribution therapeutic performance Se‐LEC NPs due its capability react ROS. The fabricated largely accumulate liver, inhibit release inflammatory cytokines, thus exerting beneficial efficacy on HIRI. This work may open new avenue design self‐assembled treatment HIRI other ROS‐related diseases.

Language: Английский

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Research Progress of Antioxidants in Oxidative Stress Therapy after Spinal Cord Injury

Neurochemical Research, Journal Year: 2023, Volume and Issue: 48(12), P. 3473 - 3484

Published: Aug. 1, 2023

Language: Английский

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