Life Sciences, Journal Year: 2022, Volume and Issue: 312, P. 121206 - 121206
Published: Nov. 18, 2022
Language: Английский
Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(5), P. 1486 - 1486
Published: May 13, 2023
Mesenchymal stromal/stem cells (MSCs) and their secreted factors have been shown to immunomodulatory regenerative effects. In this study, we investigated human bone-marrow-derived MSC secretome (MSC-S) for the treatment of corneal epithelial wounds. Specifically, evaluated role extracellular vesicles (EV)/exosomes in mediating wound-healing effects MSC-S. vitro studies using showed that MSC-CM increased cell proliferation HCEC HCLE cells, while EV-depleted lower both lines compared group. vivo experiments revealed 1X MSC-S consistently promoted wound healing more effectively than 0.5X MSC-S, a dose-dependent manner, exosome deprivation delayed healing. We further incubation period on collected 72 h is effective 48 h. Finally, stability under different storage conditions found after one cycle freeze–thawing, stable at 4 °C up weeks. Collectively, identified following: (i) MSC-EV/Exo as active ingredient mediates epithelium, providing measure optimize its dosing potential clinical product; (ii) Treatment with EV/Exo-containing resulted an improved barrier decreased haze/edema relative EV/Exo-depleted MSC-S; (iii) The weeks regular condition did not significantly impact therapeutic functions.
Language: Английский
Citations
22
Biology, Journal Year: 2023, Volume and Issue: 12(5), P. 653 - 653
Published: April 26, 2023
Spinal cord injury (SCI) represents a significant medical challenge, often resulting in permanent disability and severely impacting the quality of life for affected individuals. Traditional treatment options remain limited, underscoring need novel therapeutic approaches. In recent years, multipotent mesenchymal stem cells (MSCs) have emerged as promising candidate SCI due to their multifaceted regenerative capabilities. This comprehensive review synthesizes current understanding molecular mechanisms underlying MSC-mediated tissue repair SCI. Key discussed include neuroprotection through secretion growth factors cytokines, promotion neuronal regeneration via MSC differentiation into neural cell types, angiogenesis release pro-angiogenic factors, immunomodulation by modulating immune activity, axonal driven neurotrophic glial scar reduction modulation extracellular matrix components. Additionally, examines various clinical applications MSCs treatment, such direct transplantation injured spinal cord, engineering using biomaterial scaffolds that support survival integration, innovative cell-based therapies like MSC-derived exosomes, which possess neuroprotective properties. As field progresses, it is crucial address challenges associated with MSC-based therapies, including determining optimal sources, intervention timing, delivery methods, well developing standardized protocols isolation, expansion, characterization. Overcoming these will facilitate translation preclinical findings practice, providing new hope improved individuals living devastating consequences
Language: Английский
Citations
20
Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 365, P. 981 - 1003
Published: Dec. 21, 2023
Language: Английский
Citations
20
Schizophrenia Bulletin, Journal Year: 2023, Volume and Issue: 50(3), P. 600 - 614
Published: Dec. 12, 2023
Abstract Background and Hypothesis Schizophrenia (SCZ) is a multifaceted mental disorder marked by spectrum of symptoms, including hallucinations, delusions, cognitive deficits, negative symptoms. Its etiology involves intricate interactions between genetic environmental factors, posing significant challenges for effective treatment. We hypothesized that intranasal administration exosomes derived from nasal olfactory mucosal mesenchymal stem cells (OM-MSCs-exos) could alleviate SCZ-like behaviors in murine model induced methylazoxymethanol (MAM). Study Design conducted comprehensive investigation to assess the impact intranasally delivered OM-MSC-exos on MAM-induced mice. This study encompassed behavioral assessments, neuroinflammatory markers, glial activation, synaptic protein expression, neurogenesis within hippocampus. Results Our findings demonstrated effectively ameliorated behaviors, specifically addressing social withdrawal sensory gating deficits model. Furthermore, intervention yielded reduction markers suppression microglial activation Simultaneously, we observed an upregulation key PSD95 TH, rate-limiting enzyme dopamine biosynthesis. Conclusions underscores therapeutic potential mitigating behavior. The have capacity modulate cell diminish neuroinflammation, promote BDNF-associated plasticity neurogenesis, thus ameliorating behaviors. In summary, offers approach address SCZ mechanisms, promising innovative treatments this disorder.
Language: Английский
Citations
17
Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 3307 - 3334
Published: May 1, 2024
Abstract: Inflammatory bowel disease (IBD) is a chronic inflammatory of the gastrointestinal tract, which has high recurrence rate and incurable due to lack effective treatment. Mesenchymal stromal cells (MSCs) are class pluripotent stem that have recently received lot attention their strong self-renewal ability immunomodulatory effects, large number experimental clinical models confirmed positive therapeutic effect MSCs on IBD. In preclinical studies, MSC treatment for IBD relies paracrine cell-to-cell contact, its mediated mitochondrial transfer immune regulation. It also plays role in restoring intestinal mucosal barrier through homing effect, regulation microbiome, repair epithelial cells. latest trials, safety efficacy been by transfusion autologous or allogeneic bone marrow, umbilical cord, adipose MSCs, as well derived extracellular vesicles. However, regarding stable use several concerns emerge, including cell sources, management (dose, route frequency administration, pretreatment MSCs) adverse reactions. This article comprehensively summarizes effects mechanisms advantages over conventional drugs, trial progress The current challenges future directions discussed. review would add knowledge into understanding applying MSCs. Keywords: mesenchymal cells, immunomodulation, disease, ulcerative colitis, Crohn's therapy
Language: Английский
Citations
7
American Journal of Stem Cells, Journal Year: 2024, Volume and Issue: 13(2), P. 37 - 58
Published: Jan. 1, 2024
Recent studies demonstrated that mesenchymal stem cells (MSCs) are important for the cell-based therapy of diseased or injured lung due to their immunomodulatory and regenerative properties as well limited side effects in experimental animal models. Preclinical have shown MSCs also a remarkable effect on immune cells, which play major roles pathogenesis multiple diseases, by modulating activity, proliferation, functions. In addition, can inhibit both infiltrated detrimental responses be used treating diseases caused virus infection such Tuberculosis SARS-COV-2. Moreover, source alveolar epithelial type 2 (AT2) cells. These MSC-derived functional AT2-like treat diminish serious disorders, including acute injury, asthma, chronic obstructive pulmonary disease (COPD), fibrosis As an alternative MSC-based therapy, extracellular vesicles derived from employed medicine. Herein, we discussed key research findings recent clinical preclinical functions some common well-studied diseases. We mechanisms underlying employment attenuation injury/inflammation promotion regeneration after injury. Finally, described role pneumonia, COPD, idiopathic (IPF).
Language: Английский
Citations
6
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: Oct. 26, 2024
Radiation-induced lung injury (RILI) is associated with alveolar epithelial cell death and secondary fibrosis in injured lung. Mesenchymal stem (MSC)-derived exosomes have regenerative effect against the potential to intervene of RILI. However, their intervention efficacy limited because they lack targeting characters do not carry sufficient specific effectors. SARS-CoV-2 spike glycoprotein (SARS-CoV-2-S-RBD) binds angiotensin-converting enzyme 2 (ACE2) receptor mediates interaction host cells. MiR-486-5p a multifunctional miRNA angiogenic antifibrotic acts as an effector MSC-derived exosomes. Ferroptosis form radiation injury, its roles mechanisms RILI remain unclear. In this study, we developed engineered SARS-CoV-2-S-RBD- miR-486-5p- modification investigated effects on RIPF action via suppression ferroptosis. Adenovirus-mediated gene led miR-486-5p overexpression human umbilical cord MSC (p < 0.05), thereby constructing (miR-486-MSC-Exo). MiR-486-MSC-Exo promoted proliferation migration irradiated mouse (MLE-12) cells vitro inhibited vivo (all p 0.05). suppressed ferroptosis MLE-12 cells, assay revealed that expression fibrosis-related genes up-regulated following (both reversed induced by TGF-β1 improved pathological mice SARS-CoV-2-S-RBD-modified miR-486-5p-engineered (miR-486-RBD-MSC-Exo) were also constructed, distribution DiR dye-labeled miR-486-RBD-MSC-Exo hACE2CKI/CKI Sftpc-Cre+ demonstrated long-term retention MiR-486-RBD-MSC-Exo significantly survival rate changes Furthermore, miR-486-MSC-Exo exerted anti-fibrotic targeted SMAD2 inhibition Akt phosphorylation activation Engineered miR-486-5p-modification developed. vitro, alleviated ACE2 humanized vivo. activation. This study provides approach for intervention.
Language: Английский
Citations
6
Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 165, P. 115135 - 115135
Published: July 13, 2023
Exosomes, remarkable extracellular vesicles, have emerged as an advanced frontier in intercellular communication. This capacity positions them promising contenders drug delivery systems (DDSs) for osteoarthritis (OA) therapy, capitalizing on their inherent biocompatibility, stability, and minimal immunogenicity. In this comprehensive review, we summarize the emerging developments surrounding exosome-based DDSs OA therapy. Focusing exosome origins, meticulously explore diverse sources contributing to production, including invaluable stem cells, immune array of other cell types. addition, unravel underlying mechanisms action that govern these exosome-borne therapeutics, illuminating intricate interplay between exosomes recipient cells. summary, review highlights present challenges permeate Through in-depth exploration intricacies within field, aims shed light future direction OA. It serves a bridge fostering collaboration collective efforts reshaping treatment landscape
Language: Английский
Citations
15
Advanced Science, Journal Year: 2023, Volume and Issue: 10(19)
Published: May 5, 2023
The 2019 novel coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) is ongoing, and has necessitated scientific efforts in diagnosis, treatment, prevention. Interestingly, extracellular vesicles (EVs) have been crucial these developments. EVs are a collection of various nanovesicles which delimited lipid bilayer. They enriched proteins, nucleic acids, lipids, metabolites, naturally released from different cells. Their natural material transport properties, inherent long-term recycling ability, excellent biocompatibility, editable targeting, inheritance parental cell properties make one the most promising next-generation drug delivery nanocarriers active biologics. During COVID-19 pandemic, many made to exploit payload for treatment COVID-19. Furthermore, strategies that use engineered manufacture vaccines neutralization traps produced efficacy animal experiments clinical trials. Here, recent literature on application damage repair, prevention reviewed. And therapeutic value, strategies, safety, biotoxicity production applications EV agents as well inspiration using block eliminate viruses discussed.
Language: Английский
Citations
14
Frontiers in Aging Neuroscience, Journal Year: 2024, Volume and Issue: 16
Published: Feb. 8, 2024
Vascular dementia (VD) is a prevalent cognitive disorder among the elderly. Its pathological mechanism encompasses neuronal damage, synaptic dysfunction, vascular abnormalities, neuroinflammation, and oxidative stress, others. In recent years, extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have garnered significant attention as an emerging therapeutic strategy. Current research indicates that MSC-derived (MSC-EVs) play pivotal role in both diagnosis treatment of VD. Thus, this article delves into advancements MSC-EVs VD, discussing mechanisms by which EVs influence pathophysiological processes These form theoretical foundation for their neuroprotective effect VD treatment. Additionally, highlights potential applications diagnosis. conclusion, present promising innovative strategy With rigorous ongoing innovation, concept can transition practical clinical treatment, providing more effective options patients.
Language: Английский
Citations
5