Effect of lipopolysaccharide on TAK1-mediated hepatocyte PANoptosis through Toll-like receptor 4 during acute liver failure

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 129, P. 111612 - 111612

Published: Feb. 8, 2024

Language: Английский

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Apoptosis‐Sensitizing Tumor Nanomedicine by Regulating Pyroptosis‐Associated Inflammatory Cell Death

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(44)

Published: May 25, 2024

Abstract The vigorous development of cancer nanomedicine has revolutionized traditional oncology medicine, but it is also limited by the continuous mutation cunning cells, leading to apoptosis insensitivity and therapeutic disappointment. Inflammatory‐regulated cell death (RCD), especially pyroptosis‐related death, demonstrates huge potential for sensitization due its unique biochemical characteristics. aim this research present a thorough synopsis current knowledge on pyroptosis‐associated inflammatory including pyroptosis, cuproptosis, PANoptosis, synergistic function in nano therapy. Paradigm studies death‐mediated apoptosis‐sensitizing tumor nanotherapeutics are introduced detail, coordination mechanisms based nanomaterials discussed. In addition, multi‐angle analysis future prospects pyroptosis‐sensitized various emphasized further expand application scope RCD. It believed that emerging auxiliary treatments RCD will greatly promote progress nanomedicine.

Language: Английский

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Caspase-8 in inflammatory diseases: a potential therapeutic target

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: Oct. 8, 2024

Caspase-8, a renowned cysteine-aspartic protease within its enzyme family, initially garnered attention for regulatory role in extrinsic apoptosis. With advancing research, growing body of evidence has substantiated involvement other cell death processes, such as pyroptosis and necroptosis, well modulatory effects on inflammasomes proinflammatory cytokines. PANoptosis, an emerging concept death, encompasses pyroptosis, apoptosis, providing insight into the often overlapping cellular mortality observed during disease progression. The activation or deficiency caspase-8 enzymatic activity is closely linked to positioning key regulator survival across various physiological pathological processes. Aberrant expression associated with development progression range inflammatory diseases, including immune system disorders, neurodegenerative diseases (NDDs), sepsis, cancer. This paper delves impact these conditions, aiming elucidate potential therapeutic strategies future intervention.

Language: Английский

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Generation of Anti‐Mastitis Gene‐Edited Dairy Goats with Enhancing Lysozyme Expression by Inflammatory Regulatory Sequence using ISDra2‐TnpB System

Advanced Science, Journal Year: 2024, Volume and Issue: 11(38)

Published: Aug. 5, 2024

Gene-editing technology has become a transformative tool for the precise manipulation of biological genomes and holds great significance in field animal disease-resistant breeding. Mastitis, prevalent disease husbandry, imposes substantial economic burden on global dairy industry. In this study, regulatory sequence gene editing breeding strategy successful creation gene-edited (GED) goats with enhanced mastitis resistance using ISDra2-TnpB system as model is proposed. This included targeted integration an innate inflammatory (IRS) into promoter region lysozyme (LYZ) gene. Upon Escherichia Coli (E. coli) mammary gland infection, GED exhibited increased LYZ expression, showing robust anti-mastitis capabilities, mitigating PANoptosis activation, alleviating blood-milk-barrier (BMB) damage. Notably, highly expressed only E. coli infection. study marks advent animals exogenous-free expression demonstrates feasibility gene-editing proposed study. addition, it provides novel blueprint developing strains, focusing specificity biosafety while providing research basis widespread application system.

Language: Английский

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Rare earth regulatory defect engineering: A multifunctional nanoplatform for breast cancer therapy through PANoptosis

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 477, P. 147056 - 147056

Published: Nov. 1, 2023

Language: Английский

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Exploration and breakthrough in the mode of chondrocyte death - A potential new mechanism for osteoarthritis

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 170, P. 115990 - 115990

Published: Dec. 6, 2023

Osteoarthritis (OA) is a frequent chronic joint disease in orthopedics that effects individuals and society significantly. Obesity, aging, genetic susceptibility, misalignment are all known risk factors for OA, but its pathomechanism still poorly understood. Researches have revealed OA much complex process related to inflammation, metabolic chondrocyte death. It can affect parts of the characterized by causing death extracellular matrix descent. Previously, was thought develop from excessive mechanical loading leading destruction articular cartilage. Since some programmed cell deaths share pattern destruction, it likely also involves Even though apoptosis pyroptosis been investigated clarifing solely conventional pathways insufficient understand pathophysiology osteoarthritis. With more researches, has discovered osteoarthritis other new processes, including PANoptosis, ferroptosis, senescence, strongly associated. Among these, PANoptosis combines key traits pyroptosis, apoptosis, necrotic into highly coordinated dynamically balanced inflammatory mechanism. Furthermore, we think PANopotosis might obstruct necroptosis senescence. Therefore, order offer direction therapeutic treatment, evaluate development research on multiple chondrocytes OA.

Language: Английский

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Titanium dioxide nanoparticles induce apoptosis through ROS‐Ca²⁺‐p38/AKT/mTOR pathway in TM4 cells

Journal of Applied Toxicology, Journal Year: 2024, Volume and Issue: 44(6), P. 818 - 832

Published: Jan. 25, 2024

Abstract Titanium dioxide nanoparticles (TiO 2 NPs) can cause apoptosis in TM4 cells; however, the underlying mechanism has not been entirely elucidated. The purpose of this study was to investigate effects TiO NPs on ROS, Ca 2+ level, p38/AKT/mTOR pathway, and cells evaluate role pathway apoptosis. After exposure different concentrations (0, 50, 100, 150, 200 μg/mL) for 24 h, cell viability, ‐ATPase activity, pathway‐related proteins, rate, apoptosis‐related proteins (Bax, Bcl‐2, Caspase 3, 9, p53) were detected. ROS scavenger NAC used determine effect level. chelator BAPTA‐AM significantly inhibited increased elevated level while suppressing activity. regulated via increasing p‐p38 decreasing p‐AKT p‐mTOR levels. enhanced attenuated overload reduction activity caused by NPs. alleviated NPs‐induced abnormal expression proteins. assuaged Altogether, revealed that intracellular through accumulation. Subsequently, heightened observed exert regulation over ultimately culminating These results provides a complementary understanding cells.

Language: Английский

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A2 reactive astrocyte‐derived exosomes alleviate cerebral ischemia–reperfusion injury by delivering miR‐628

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(16)

Published: Aug. 1, 2024

Abstract Ischemia and hypoxia activate astrocytes into reactive types A1 A2, which play roles in damage protection, respectively. However, the function mechanism of A2 astrocyte exosomes are unknown. After were injected lateral ventricle, infarct volume, to blood–brain barrier (BBB), apoptosis expression microglia‐related proteins measured. The dual luciferase reporter assay was used detect target genes miR‐628, overexpressing A2‐Exos overexpressed knocked down miR‐628 constructed. qRT–PCR, western blotting immunofluorescence staining subsequently performed. obviously reduced BBB promoted M2 microglial polarization. RT–PCR showed that highly expressed A2‐Exos. Dual assays revealed NLRP3, S1PR3 IRF5 miR‐628. or down, protective effects increased decreased, pyroptosis polarization through inhibition via delivery This study explored action provided new therapeutic targets concepts for treating cerebral ischemia.

Language: Английский

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PLA inhibits TNF-α-induced PANoptosis of prostate cancer cells through metabolic reprogramming

The International Journal of Biochemistry & Cell Biology, Journal Year: 2024, Volume and Issue: 169, P. 106554 - 106554

Published: Feb. 24, 2024

Language: Английский

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Diabetic peripheral neuropathy based on Schwann cell injury: mechanisms of cell death regulation and therapeutic perspectives

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 12, 2024

Diabetic peripheral neuropathy (DPN) is a complication of diabetes mellitus that lacks specific treatment, its high prevalence and disabling neuropathic pain greatly affects patients’ physical mental health. Schwann cells (SCs) are the major glial nervous system, which play an important role in various inflammatory metabolic neuropathies by providing nutritional support, wrapping axons promoting repair regeneration. Increasingly, glucose (HG) has been found to promote progression DPN pathogenesis targeting SCs death regulation, thus revealing molecular process programmed cell (PCD) disrupted link gain insight into DPN. This paper first review recent progress HG studies on apoptosis, autophagy, pyroptosis, ferroptosis necroptosis pathways SCs, points out crosstalk between PCDs related therapeutic perspectives, with aim new perspectives for deeper understanding mechanisms exploration effective targets.

Language: Английский

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