POLE -related gene signature predicts prognosis, immune feature, and drug therapy in human endometrioid carcinoma DOI Creative Commons
Wei Qiu, Runjie Zhang, Ying-Chen Qian

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(8), P. e29548 - e29548

Published: April 1, 2024

The POLE subtype of Endometrial carcinoma (EC) is linked to a favourable prognosis in the molecular classification. We proposed ascertain potential connection between and improved prognosis. In order forecast prognosis, least absolute shrinkage selection operator (LASSO) Cox regression analysis weighted gene co-expression network (WGCNA) were employed, POLE-related risk signature (PRS) model was developed validated. Single-sample set enrichment (ssGSEA) with "GSVA" package employed analyse immunity characteristics. Drug susceptibility studies conducted compare half-maximal inhibitory concentration (IC50) medicines high- low-risk groups. PRS generated employing LASSO coefficients ELF1, MMADHC, AL021707.6 genes. Our study demonstrated that score tumour stage, grade, survival. Furthermore, group possessed elevated levels expression connected immunological checkpoints HLA. outcomes emerged might have value identifying patients good facilitating personalised treatment clinic.

Language: Английский

An updated review on abnormal epigenetic modifications in the pathogenesis of systemic lupus erythematosus DOI Creative Commons
Xingyu Zhou, Shengnan Zhou, Yaping Li

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 6, 2025

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease. The inconsistent prevalence of SLE between monozygotic twins suggests that environmental factors affect the occurrence this Abnormal epigenetic regulation strongly associated with pathogenesis SLE. Epigenetic mechanisms may be involved in development through DNA methylation, histone modification, noncoding RNAs, and other modifications. This review aims to show numerous studies as treasure map better understand effects aberrant modification onset SLE, which will benefit current basic research provide potential diagnostic biomarkers or therapeutic targets for

Language: Английский

Citations

1
Identification of m6A-related biomarkers in Kawasaki disease DOI
Xu Xiao, Min Wang, Zhimin Geng

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2025, Volume and Issue: unknown, P. 167744 - 167744

Published: Feb. 1, 2025

Language: Английский

Citations

0
Research and analysis of differential gene expression in CD34 hematopoietic stem cells in myelodysplastic syndromes DOI Creative Commons
Mianzhi Wang, Chang-Sheng Liao, Xudong Wei

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0315408 - e0315408

Published: March 12, 2025

Objective This study aims to investigate and analyze the differentially expressed genes (DEGs) in CD34 + hematopoietic stem cells (HSCs) from patients with myelodysplastic syndromes (MDS) through bioinformatics analysis, ultimate goal of uncovering potential molecular mechanisms underlying pathogenesis MDS. The findings this are expected provide novel insights into clinical treatment strategies for Methods Initially, we downloaded three datasets, GSE81173, GSE4619, GSE58831, public Gene Expression Omnibus (GEO) database as our training sets, selected GSE19429 dataset validation set. To ensure data consistency comparability, standardized sets removed batch effects using ComBat algorithm, thereby integrating them a unified gene expression dataset. Subsequently, conducted differential analysis identify significant changes levels across different disease states. In order enhance prediction accuracy, incorporated six common predictive models trained based on filtered After comprehensive evaluation, ultimately algorithms—Lasso regression, random forest, support vector machine (SVM)—as core models. more precisely pinpoint closely related characteristics, utilized aforementioned learning methods took intersection these results, yielding robust list associated features. Following this, in-depth key set validated results independently Furthermore, performed groups, co-expression enrichment delve deeper roles initiation progression. Through analyses, aim new foundations diagnosis treatment. Figure illustrates preprocessing workflow study. Results Our CD34+ revealed differences patterns compared control group (individuals without MDS). Specifically, two genes, IRF4 ELANE, were notably downregulated HSCs MDS patients, indicating their downregulatory pathological process Conclusion sheds light MDS, particular focus pivotal ELANE pathogenic genes. perspective understanding complexity exploring therapeutic strategies. They may also guide development precise effective treatments, such targeted interventions directed against

Language: Английский

Citations

0
Biomarkers for systemic lupus erythematosus: A scoping review DOI Creative Commons

Su‐jie Zhang,

R.C. Xu, Longli Kang

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(10)

Published: Oct. 1, 2024

Abstract Background In recent years, newly discovered potential biomarkers have great research in the diagnosis, disease activity prediction, and treatment of systemic lupus erythematosus (SLE). Objective this study, a scoping review for SLE over several years has identified extent to which studies on been conducted, specificity, sensitivity, diagnostic value SLE, these detection is discussed. Methods PubMed Google Scholar databases, “SLE,” “biomarkers,” “predictor,” “autoimmune diseases,” “lupus nephritis,” “neuropsychiatric SLE,” “diagnosis,” “monitoring,” “disease activity” were used as keywords systematically search molecular published from 2020 2024. Analyze summarize literature that can guide article. Conclusions Recent findings suggest some may clinical application prospects. However, date, many not subjected repeated validation. And no single biomarker sufficient sensitivity specificity SLE. It scientific choose only one or judge complex be good direction carry out meta‐analysis various find suitable use, evaluate by combining multiple through mathematical models. At same time, advanced computational methods are needed analyze large data sets discover new biomarkers, strive sensitive specific enough practice, rather than staying experimental analysis.

Language: Английский

Citations

2
The Roles of RNA N6-methyladenosine Modifications in Systemic Lupus Erythematosus DOI
Xin Xia, Rui Qu

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(4), P. 3223 - 3234

Published: Aug. 2, 2024

Language: Английский

Citations

1
POLE -related gene signature predicts prognosis, immune feature, and drug therapy in human endometrioid carcinoma DOI Creative Commons
Wei Qiu, Runjie Zhang, Ying-Chen Qian

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(8), P. e29548 - e29548

Published: April 1, 2024

The POLE subtype of Endometrial carcinoma (EC) is linked to a favourable prognosis in the molecular classification. We proposed ascertain potential connection between and improved prognosis. In order forecast prognosis, least absolute shrinkage selection operator (LASSO) Cox regression analysis weighted gene co-expression network (WGCNA) were employed, POLE-related risk signature (PRS) model was developed validated. Single-sample set enrichment (ssGSEA) with "GSVA" package employed analyse immunity characteristics. Drug susceptibility studies conducted compare half-maximal inhibitory concentration (IC50) medicines high- low-risk groups. PRS generated employing LASSO coefficients ELF1, MMADHC, AL021707.6 genes. Our study demonstrated that score tumour stage, grade, survival. Furthermore, group possessed elevated levels expression connected immunological checkpoints HLA. outcomes emerged might have value identifying patients good facilitating personalised treatment clinic.

Language: Английский

Citations

0