Revolutionizing stem cell research: unbiased insights through single-cell sequencing DOI Open Access
Hao Wu,

NA HUO,

S.J. Wang

et al.

Biocell, Journal Year: 2024, Volume and Issue: 48(11), P. 1531 - 1542

Published: Jan. 1, 2024

Stem cells have shown great application potential in wound repair, tissue regeneration, and disease treatment.Therefore, a full understanding of stem their related regulatory mechanisms treatment is conducive to improving the therapeutic effect cells.However, thus far, there are still many unsolved mysteries field due technical limitations, which hinder in-depth exploration wide clinical application.Single-cell sequencing (SCS) has provided very powerful unbiased insights into cell gene expression profiles at single-cell level, bringing exciting results field.At present, SCS been widely applied cells, covering various aspects, including lineage tracing development identifying new types, exploring cellular heterogeneity, internal functional subpopulations.In this paper, we focus on latest research progress discuss technology cells.

Language: Английский

IGFBP5 is released by senescent cells and is internalized by healthy cells, promoting their senescence through interaction with retinoic receptors DOI Creative Commons
Nicola Alessio, Domenico Aprile, Gianfranco Peluso

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Feb. 13, 2024

Abstract Cells that are exposed to harmful genetic damage, either from internal or external sources, may undergo senescence if they unable repair their DNA. Senescence, characterized by a state of irreversible growth arrest, can spread neighboring cells through process known as the senescence-associated secretory phenotype (SASP). This phenomenon contributes both aging and development cancer. The SASP comprises variety factors regulate numerous functions, including induction secondary senescence, modulation immune system activity, remodeling extracellular matrix, alteration tissue structure, promotion cancer progression. Identifying key within is crucial for understanding underlying mechanisms developing effective strategies counteract cellular senescence. Our research has specifically focused on investigating role IGFBP5, component observed in various experimental models conditions. Through our studies, we have demonstrated IGFBP5 actively promoting induce cells. We gained valuable insights into which exerts its pro-senescence effects. These include release following genotoxic stress, involvement signaling pathways mediated reactive oxygen species prostaglandins, internalization via specialized structures called caveolae, interaction with specific protein RARα. By uncovering these mechanisms, advanced intricate process. significance pro-aging factor stems an vivo study conducted patients undergoing Computer Tomography analysis. In patients, elevation circulating levels response radiation-induced organismal stress. Globally, findings highlight potential promising therapeutic target age-related diseases

Language: Английский

Citations

10
Non-small cell lung cancer cells and concomitant cancer therapy induce a resistance-promoting phenotype of tumor-associated mesenchymal stem cells DOI Creative Commons

Hanna Sentek,

Annika Braun, Bettina Budeus

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 1, 2024

The tumor microenvironment gained attraction over the last decades as stromal cells significantly impact on development, progression and metastasis, immune evasion well cancer therapy resistance. We previously reported that lung-resident mesenchymal stem (MSCs) were mobilized activated in non-small cell lung (NSCLC) could even mediate radiation resistance co-cultured NSCLC cells.

Language: Английский

Citations

5
Fucoxanthin diminishes oxidative stress damage in human placenta-derived mesenchymal stem cells through the PI3K/Akt/Nrf-2 pathway DOI Creative Commons

Gunticha Suwanmanee,

Chairat Tantrawatpan, Pakpoom Kheolamai

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 27, 2023

Abstract Placenta-derived mesenchymal stem cells (PL-MSCs) have therapeutic potential in various clinical contexts due to their regenerative and immunomodulatory properties. However, with increasing age or extensive vitro culture, viability function are gradually lost, thus restricting application. The primary cause of this deterioration is oxidative injury from free radicals. Therefore, enhancing cell restoring cellular repair mechanisms PL-MSCs an stress environment crucial context. Fucoxanthin, a carotenoid derived brown seaweed, demonstrates antioxidant activity by the production enzymes lowering levels reactive oxygen species (ROS). This study aimed determine whether fucoxanthin protects hydrogen peroxide (H 2 O )-induced stress. After characterization, were co-treated H for 24 h (co-treatment) pre-treated followed (pre-treatment). effects on proliferation examined using MTT assay. expression enzymes, PI3K/Akt/Nrf-2 intracellular ROS investigated fucoxanthin-treated compared untreated group. gene involvement specific pathways cytoprotective effect high-throughput NanoString nCounter analysis. results demonstrated that co-treatment pre-treatment restored proliferative capacity PL-MSCs. Fucoxanthin treatment increased cultured under conditions decreased accumulation. Markedly, could restore -treated High-throughput analysis revealed up-regulation genes involved survival pathways, including cycle proliferation, DNA damage down-regulation apoptosis autophagy pathways. rescues through pathway. Our data provide supporting evidence use as agent improve both vivo required increase effectiveness MSC expansion applications.

Language: Английский

Citations

12
Combining rejuvenation interventions in rodents: a milestone in biomedical gerontology whose time has come DOI Creative Commons
Caitlin J. Lewis, Aubrey D.N.J. de Grey

Expert Opinion on Therapeutic Targets, Journal Year: 2024, Volume and Issue: 28(6), P. 501 - 511

Published: March 13, 2024

Introduction Longevity research has matured to the point where significantly postponing age-related decline in physical and mental function is now achievable laboratory foreseeable clinic. The most promising strategies involve rejuvenation, i.e. reducing biological age, not merely slowing its progression.

Language: Английский

Citations

4
Mesenchymal stromal cell exosomes for drug delivery of prostate cancer treatments: a review DOI Creative Commons

Chengran Wang,

Yanling Feng,

Xianfang Rong

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 23, 2025

Interest in prostate cancer as a research topic has gradually increased. As result, series of innovative treatment strategies have emerged with an in-depth understanding the disease. Owing to their unique biological characteristics, mesenchymal stromal cell exosomes (MSC-Exos) garnered significant attention for potential deliver targeted drugs and enable precise treatment. Herein, MSC-Exos drug-delivery systems is reviewed. This review provides comprehensive introduction advantages these systems, current trends progress, well analysis challenges future directions. Moreover, this lays solid foundation continued development application MSC-Exos.

Language: Английский

Citations

0
The role of different physical exercises as an anti-aging factor in different stem cells DOI
Jiajia Xu, Zhe Song

Biogerontology, Journal Year: 2025, Volume and Issue: 26(2)

Published: Feb. 26, 2025

Language: Английский

Citations

0
Mesenchymal stem cells and their derivatives as potential longevity-promoting tools DOI Creative Commons

Ekaterina Rudnitsky,

Alex Braiman,

Marina Wolfson

et al.

Biogerontology, Journal Year: 2025, Volume and Issue: 26(3)

Published: April 21, 2025

Abstract Mesenchymal stem cells (MSCs) and blood plasma/MSC-derived extracellular vesicles (EVs) offer promising tools to promote longevity treat age-related diseases. MSCs have low immunogenicity tumorigenicity, their efficacy is relatively independent of the donor age in humans (but not rodents). Systemic administration cell/blood-derived EVs modified omic profiles various organs aged rodents towards young ones. The application appears be even more beneficial than MSCs. Remarkably, over 70% microRNAs, which are over-presented ESC-derived EVs, were found target longevity-associated genes. Along with MSCs, other types reported display health- lifespan-extending effects. Pluripotent Muse cells, a specific subpopulation possess number unique features, could particularly relevant for promoting healthspan. rejuvenation potential warrants further investigation both animal models clinical trials, using aging clocks biological determination as one endpoints.

Language: Английский

Citations

0
Mesenchymal stem cells and their derived exosomes in multiple sclerosis disease: from paper to practice DOI
Seyed Hootan Hamidi,

Negar Etebar,

Milad Rahimzadegan

et al.

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: 479(7), P. 1643 - 1671

Published: July 1, 2024

Language: Английский

Citations

2
Cellular microenvironment: a key for tuning mesenchymal stem cell senescence DOI Creative Commons

Wenyang Sun,

Jiacheng Lv,

Shu Guo

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Dec. 4, 2023

Mesenchymal stem cells (MSCs) possess the ability to self-renew and differentiate into multiple cell types, making them highly suitable for use as seed in tissue engineering. These can be derived from various sources have been found play crucial roles several physiological processes, such repair, immune regulation, intercellular communication. However, limited capacity proliferation secretion of senescence-associated secreted phenotypes (SASPs) pose challenges clinical application MSCs. In this review, we provide a comprehensive summary senescence characteristics MSCs examine different features cellular microenvironments studied thus far. Additionally, discuss mechanisms by which regulate process MSCs, offering insights preserving their functionality enhancing effectiveness.

Language: Английский

Citations

4
Human gingival mesenchymal stem cells-lyosecretome attenuates adverse effect of hydrogen peroxide-induced oxidative stress on osteoblast cells DOI Creative Commons
Banun Kusumawardani, Muhammad Nurul Amin, Yani Corvianindya Rahayu

et al.

Journal of Taibah University Medical Sciences, Journal Year: 2024, Volume and Issue: 19(3), P. 687 - 695

Published: May 13, 2024

To determine total protein content, antioxidant activity, and protective ability of lyophilized human gingival mesenchymal stem cells (hGMSCs)-secretome in hydrogen peroxide (H

Language: Английский

Citations

0