Applications of mesenchymal stem cell-exosome components in wound infection healing: new insights

Burns & Trauma, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 1, 2024

Abstract The healing process at a wound is made up of many types cells, growth factors, the extracellular matrix, nerves and blood vessels all interacting with each other in complex changing ways. Microbial colonization proliferation are possible place injury, which makes infection more likely. Because this, any cut has chance getting an infection. Researchers have found that infections make patients upset cost healthcare system lot money. Surgical site happen to people who recently had surgery. This study shows such surgical linked high rate illness death. shown by fact 25% get serious sepsis need be transferred intensive care unit. In both animal models people, mesenchymal stem cells (MSCs) play active role stages positive effects. Exosomes one main things MSCs release. They effects similar those parent MSCs. Various effector proteins, messenger RNA microRNAs can transported vesicles control activity target cells. big impact on process. These results suggest using MSC-exosomes as new type cell-free therapy could better safer option than whole cell therapy. review mostly about how use parts help heal.

Language: Английский

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Optimizing mesenchymal stem cell extracellular vesicles for chronic wound healing: Bioengineering, standardization, and safety

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 260 - 274

Published: June 1, 2024

Chronic wounds represent a significant global burden, afflicting millions with debilitating complications. Despite standard care, impaired healing persists due to factors like persistent inflammation and tissue regeneration. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) offer an innovative regenerative medicine approach, delivering cell-derived therapeutic cargo in engineered nanoscale delivery systems. This review examines pioneering bioengineering strategies engineer MSC-EVs into precision nanotherapeutics for chronic wounds. Emerging technologies CRISPR gene editing, microfluidic manufacturing, biomimetic systems are highlighted their potential enhance MSC-EV targeting, optimize enrichment, ensure consistent clinical-grade production. However, key hurdles remain, including batch variability, rigorous safety assessment tumorigenicity, immunogenicity, biodistribution profiling. Crucially, collaborative frameworks harmonizing regulatory science patient advocacy hold the expediting clinical translation. By overcoming these challenges, could catalyze new era of off-the-shelf therapies, restoring hope afflicted by non-healing

Language: Английский

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Stem Cell-Derived Extracellular Vesicle-Mediated Therapeutic Signaling in Spinal Cord Injury

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 723 - 723

Published: Jan. 16, 2025

Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have emerged as a promising therapeutic strategy for spinal cord injury (SCI). These nanosized possess unique properties such low immunogenicity and the ability to cross biological barriers, making them ideal carriers delivering bioactive molecules injured tissues. MSC-EVs been demonstrated exert multiple beneficial effects in SCI, including reducing inflammation, promoting neuroprotection, enhancing axonal regeneration. Recent studies delved into molecular mechanisms underlying MSC-EV-mediated effects. Exosomal microRNAs (miRNAs) identified key regulators of various cellular processes involved SCI pathogenesis repair. miRNAs can influence oxidative stress, apoptosis by modulating gene expression. This review summarized current state MSC-EV-based therapies highlighting potential clinical applications. We discussed challenges limitations translating these practice, inconsistent EV production, complex cargo composition, need targeted delivery strategies. Future research should focus on optimizing production characterization, identifying miRNAs, developing innovative systems maximize SCI.

Language: Английский

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Clinical Efficacy of Extracellular Vesicle Therapy in Periodontitis: Reduced Inflammation and Enhanced Regeneration

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5753 - 5753

Published: May 25, 2024

Periodontitis, a prevalent inflammatory condition, affects the supporting structures of teeth, leading to significant oral health challenges. Traditional treatments have primarily focused on mechanical debridement, antimicrobial therapy, and surgery, which often fail restore lost periodontal structures. Emerging as novel approach in regenerative medicine, extracellular vesicle (EV) including exosomes, leverages nano-sized vesicles known for facilitating intercellular communication modulating physiological pathological processes. This study is proof-of-concept type that evaluates clinical efficacy EV therapy non-surgical treatment stage I–III periodontitis, focusing its anti-inflammatory potential. The research involved seven patients undergoing healthy individuals. Clinical parameters, plaque index, bleeding probing, probing depth, attachment level, were assessed alongside cytokine levels gingival crevicular fluid. found improvements marked reduction pro-inflammatory cytokines post-treatment, matching subjects, underscoring therapy’s ability not only attenuate inflammation enhance tissue regeneration, but also highlighting potential restoring health. investigation illuminates promising role treatment, advocating shift towards therapies halt disease progression promote structural functional restoration tissues.

Language: Английский

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Exosome Mediated Cell-Cell Crosstalk in Tissue Injury and Repair

Results and problems in cell differentiation, Journal Year: 2024, Volume and Issue: unknown, P. 249 - 297

Published: Jan. 1, 2024

Language: Английский

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Recent progress in engineered extracellular vesicles and their biomedical applications

Life Sciences, Journal Year: 2024, Volume and Issue: 350, P. 122747 - 122747

Published: May 24, 2024

Language: Английский

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Designer Engineered Exosomes: a New Regenerative Medicine Platform for Skin Wound Healing

Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Language: Английский

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Nanomanaging Chronic Wounds with Targeted Exosome Therapeutics

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 366 - 366

Published: March 13, 2025

Chronic wounds pose a significant healthcare challenge, impacting millions of patients worldwide and burdening systems substantially. These often occur as comorbidities are prone to infections. Such infections hinder the healing process, complicating clinical management proving recalcitrant therapy. The environment within wound itself poses challenges such lack oxygen, restricted blood flow, oxidative stress, ongoing inflammation, bacterial presence. Traditional systemic treatment for chronic peripheral may not be effective due inadequate supply, resulting in unintended side effects. Furthermore, topical applications impervious persistent biofilm A growing concern is therapeutic modalities treating wounds. Additionally, chemically harsh microenvironment can reduce effectiveness treatments, highlighting need drug delivery that deliver therapies precisely where needed with optimal dosages. Compared cell-based therapies, exosome-based offer distinct advantages cell-free approach treatment. Exosomes endosomal origin enable cell-to-cell communications, they possess benefits, including biocompatibility decreased immunogenicity, making them ideal vehicles efficient targeting minimizing off-target damage. However, exosomes rapidly cleared from body, it difficult maintain concentrations at sites. hydrogel-based development biocompatible scaffolds beneficial sustained release prolong presence these Engineered have been shown stability promoting compared their unmodified counterparts. Significant progress has made this field, but further research essential unlock potential. This review seeks explore benefits opportunities wounds, ensuring efficacy precise despite obstacles posed by environment.

Language: Английский

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Extracellular vesicles: innovative cell-free solutions for wound repair

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 3, 2025

Chronic non-healing wounds are often associated with conditions such as diabetes and peripheral vascular disease, pose significant medical socioeconomic challenges. Cell-based therapies have shown promise in promoting wound healing but major drawbacks immunogenicity tumor formation. As a result, recent research has shifted to the potential of extracellular vesicles (EVs) derived from these cells. EVs nanosized lipid bilayer vesicles, naturally produced by all cell types, which facilitate intercellular communication carry bioactive molecules, offering advantages low immunogenicity, negligible toxicity be re-engineered. Recent evidence recognizes that during released wide range cells including immune cells, skin epithelial platelets they actively participate repair. This review comprehensively summarizes latest on function endogenous types different phases healing, thereby presenting interesting therapeutic targets. Additionally, it gives critical overview current status mesenchymal stem cell-derived treatment highlighting their tremendous non-cellular of-the-shelf alternative care.

Language: Английский

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Methods, Mechanisms, and Application Prospects for Enhancing Extracellular Vesicle Uptake

Current Medical Science, Journal Year: 2024, Volume and Issue: 44(2), P. 247 - 260

Published: April 1, 2024

Language: Английский

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