DDR2 alleviates retinal vaso-obliteration and pathological neovascularization by modulating microglia M1/M2 phenotypic polarization in a mouse model of proliferative retinopathy DOI Creative Commons
Qinjin Dai, Wenqi Su, Zhongcheng Zhou

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2025, Volume and Issue: 1871(5), P. 167787 - 167787

Published: March 9, 2025

Retinopathy of prematurity (ROP), a leading cause blindness in premature infants, is characterized by retinal vaso-obliteration during hyperoxia and pathological neovascularization (NV) relative hypoxia phase. Current treatments, which focus on the late stages neovascularization, are associated with numerous side effects. Studies demonstrated that discoidin domain receptor 2 (DDR2), collagen-binding tyrosine kinase, inhibits experimental choroidal participates tumor angiogenesis. However, role DDR2 ROP underlying mechanisms unclear. In this study, we initially found expressed mouse physiological vascular development significantly decreased phase followed increase oxygen-induced retinopathy (OIR) model. Early upregulation before attenuates vaso-obliteration, reduces promotes maturation. Additionally, increased number microglia around blood vessels induced anti-inflammatory M2 polarization. Furthermore, STAT6/TGF-β signaling pathway suppressed was activated after upregulation. conclusion, attenuated inhibited switching polarization from M1 to phenotype via OIR. This suggests could be novel target for early treatment ROP.

Language: Английский

Biomaterials and tissue engineering approaches using glycosaminoglycans for tissue repair: Lessons learned from the native extracellular matrix DOI Creative Commons

Roseline Menezes,

Richard Vincent, Laura Osorno

et al.

Acta Biomaterialia, Journal Year: 2022, Volume and Issue: 163, P. 210 - 227

Published: Sept. 29, 2022

Language: Английский

Citations

48
Comprehensive review of the state-of-the-art in corneal 3D bioprinting, including regulatory aspects DOI Creative Commons
Hodei Gómez-Fernández, Fouad Al-Hakim Khalak, Sandra Ruíz

et al.

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 662, P. 124510 - 124510

Published: July 24, 2024

The global shortage of corneal transplants has spurred an urgency in the quest for efficient treatments. This systematic review not only provides a concise overview current landscape morphology, physiology, diseases, and conventional treatments but crucially delves into forefront tissue engineering regeneration. Emphasizing cellular acellular components, bioprinting techniques, pertinent biological assays, it explores optimization strategies manufacturing cost-effectiveness. To bridge gap between research industrial production, outlines essential regulatory strategy required Europe, encompassing relevant directives, frameworks, governing bodies. comprehensive framework spans entire process, from procuring initial components to marketing subsequent product surveillance. In pivotal shift towards future, culminates by highlighting latest advancements this sector, particularly integration therapy with artificial intelligence. synergy promises substantial overall paving way unprecedented breakthroughs essence, elucidates state also pathways anticipates transformative impact intelligence, providing guide researchers, practitioners, policymakers field.

Language: Английский

Citations

10
Artificial Cornea Substitute Based on Hydrogel Skeletons with Natural Stromal Hierarchical Structure and Extracellular Matrix for Sutureless Transplantation DOI Creative Commons
Long Zhao,

Zhen Shi,

Xiaoyu Zhang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Abstract Corneal substitutes with structural and compositional characteristics resembling those of natural corneas have attracted considerable attention. However, biomimicking the complex hierarchical organization corneal stroma is challenging. In this study, humanized stroma‐like adhesive patches (HCSPs) are prepared through a multi‐step process. First, polyethylene glycol diacrylate cast cured within decellularized porcine cornea (DPC) templates. The DPCs then enzymatically digested to obtain hydrogel skeletons, which finally integrated human extracellular matrix methacrylate gelatin. HCSPs replicate ultrastructure, protein components, optical properties exhibit improved anti‐swelling anti‐degradation capabilities compared conventional recombinant collagen patches. can deliver gelatin at ocular surface temperature (37 °C) achieve stable adhesion upon 405 nm light irradiation. Furthermore, promote survival migration epithelial stromal cells while preserving their phenotypes. rabbit models lamellar keratoplasty microperforation repair, accelerate healing, minimize suture‐associated complications, maintain stability. These findings suggest that promising donor for clinical applications.

Language: Английский

Citations

1
Current and new therapies for mucopolysaccharidoses DOI Creative Commons
Monica Penon‐Portmann, David Blair, Paul Harmatz

et al.

Pediatrics & Neonatology, Journal Year: 2022, Volume and Issue: 64, P. S10 - S17

Published: Oct. 26, 2022

The mucopolysaccharidoses (MPSs) are a subset of lysosomal storage diseases caused by deficiencies in the enzymes required to metabolize glycosaminoglycans (GAGs), group extracellular heteropolysaccharides that play diverse roles human physiology. As result, GAGs accumulate multiple tissues, and affected patients typically develop progressive, multi-systemic symptoms early childhood. Over last 30 years, treatments available for MPSs have evolved tremendously. There now therapies delay progression these debilitating disorders, although their effectiveness varies according MPS sub-type. In this review, we discuss basic principle underlying treatment (enzymatic "cross correction"), review three general modalities currently available: hematopoietic stem cell transplantation, enzymatic replacement, gene therapy. For each type, its across subtypes, inherent risks, future directions. Long term, suspect will continue evolve, through combination diagnosis effective management, live longer lives with improved outcomes quality life.

Language: Английский

Citations

36
Selective targeting of visceral adiposity by polycation nanomedicine DOI Open Access
Qianfen Wan, Baoding Huang, Tianyu Li

et al.

Nature Nanotechnology, Journal Year: 2022, Volume and Issue: 17(12), P. 1311 - 1321

Published: Dec. 1, 2022

Language: Английский

Citations

32
Decellularization Strategies for Regenerating Cardiac and Skeletal Muscle Tissues DOI Creative Commons
Yong How Tan, Haylie R. Helms, Karina H. Nakayama

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Feb. 28, 2022

Cardiovascular disease is the leading cause of death worldwide and associated with approximately 17.9 million deaths each year. Musculoskeletal conditions affect more than 1.71 billion people globally are disability. These two areas represent a massive global health burden that perpetuated by lack functionally restorative treatment options. The fields regenerative medicine tissue engineering offer great promise for development therapies to repair damaged or diseased tissues. Decellularized tissues extracellular matrices cornerstones biomaterials have been used clinically decades many received FDA approval. In this review, we first discuss compare methods produce decellularized ECMs from cardiac skeletal muscle. We take focused look at how different biophysical properties such as spatial topography, matrix composition, mechanical characteristics influence cell behavior function in context medicine. Lastly, describe emerging research forecast future high impact applications muscle will drive novel effective therapies.

Language: Английский

Citations

31
Insights into the biochemical and biophysical mechanisms mediating the longevity of the transparent optics of the eye lens DOI Creative Commons
Roy A. Quinlan, John I. Clark

Journal of Biological Chemistry, Journal Year: 2022, Volume and Issue: 298(11), P. 102537 - 102537

Published: Sept. 27, 2022

A summary of key factors needed in the development and maintenance transparency optical properties aging lens is seen Figure 8. Interactions at molecular cellular levels account for formation transparent symmetric optics that contribute to refractive living "refracton" eye. Their significance visual function cannot be overstated. From perspective global health, these can impact progress on novel therapeutics preserve improve vision, specifically populations. As a challenging scientific problem, biology interactions between light waves organic matter basis extracellular tissues. Systematic studies are providing new knowledge about membrane, cytoskeletal, cytoplasmic adaptations, which generate image-forming refracton how it maintained matures over lifetime. The signaling mechanisms synchronize proliferation, migration, elongation, as highly optically tissue unique lens. valuable model study both longevity. This particularly true with respect protein stabilization involving PTMs, because very long-lived proteins not replaced other nontransparent tissues.Figure 8Key systematic review recent past advances cataract research provides insight into biochemical biophysical control short-range order (SRO), transparency, symmetry during aging. Note: While image largely index, linked closely protein, structure, supported by microcirculation, metabolism, biochemistry. These regulate cell elongation differentiation prolong longevity molecules cells isolated within capsule, thickest basement membrane any mammalian (102Nielsen J. Hedeholm R.B. Heinemeier Bushnell P.G. Christiansen J.S. Olsen et al.Eye radiocarbon reveals centuries Greenland shark (Somniosus microcephalus).Science. 2016; 353: 702-704Crossref PubMed Scopus (171) Google Scholar, 104Uwineza A. Kalligeraki A.A. Hamada N. Jarrin M. Quinlan R.A. Cataractogenic load - concept contribution ionizing radiation accelerated eye lens.Mutat. Res. 2019; 779: 68-81Crossref (29) 124Song S. Landsbury Dahm R. Liu Y. Zhang Q. Functions intermediate filament cytoskeleton lens.J. Clin. Invest. 2009; 119: 1837-1848Crossref (123) 184Fujii Takemoto L.J. Momose Matsumoto Hiroki K. Akaboshi Formation four isomers asp-151 residue aged human alphaA-crystallin natural aging.Biochem. Biophys. Commun. 1999; 265: 746-751Crossref (0) 290Pierscionek B.K. Regini J.W. gradient index eye: an opto-biological synchrony.Prog. Retin. Eye 2012; 31: 332-349Crossref (47) 342Subczynski W.K. Widomska Mainali L. Factors determining oxygen permeability biological membranes: transport across fiber-cell plasma membranes.Adv. Exp. Med. Biol. 2017; 977: 27-34Crossref (8) 423Patterson Characterization equatorial current lens.Ophthalmic 1988; 20: 139-142Crossref (14) 424Wolf L.V. Yang Wang Xie Braunger B. Tamm E.R. al.Identification pax6-dependent gene regulatory networks mouse lens.PLoS One. 4: e4159Crossref Scholar). Individual panels taken or adapted from Refs. (104Uwineza 131Masters P.M. Bada J.L. Zigler Jr., Aspartic acid racemization heavy weight crystallins water insoluble normal lenses cataracts.Proc. Natl. Acad. Sci. U. 1978; 75: 1204-1208Crossref 425Braakhuis A.J. Donaldson C.I. Lim J.C. P.J. Nutritional strategies prevent cataract: status future strategies.Nutrients. 11: 1186Crossref (38) 426Maisel H.C. Harding C.V. Alcala J.R. Kuszak Bradley Morphology Lens Molecular Cellular Biology Lens..in: Bloemendal H. Wiley Sons, New York1981Google Scholar); https://commons.wikimedia.org/wiki/File:Greenland_shark_profile.jpg). DF, differentiating fiber cell; MF, differentiated mature cells.View Large Image ViewerDownload Hi-res Download (PPT)

Language: Английский

Citations

30
Advances in Polysaccharide-Based Microneedle Systems for the Treatment of Ocular Diseases DOI Creative Commons

Qingdong Bao,

Xiaoting Zhang,

Zhankun Hao

et al.

Nano-Micro Letters, Journal Year: 2024, Volume and Issue: 16(1)

Published: Aug. 13, 2024

Abstract The eye, a complex organ isolated from the systemic circulation, presents significant drug delivery challenges owing to its protective mechanisms, such as blood-retinal barrier and corneal impermeability. Conventional administration methods often fail sustain therapeutic levels may compromise patient safety compliance. Polysaccharide-based microneedles (PSMNs) have emerged transformative solution for ophthalmic delivery. However, comprehensive review of PSMNs in ophthalmology has not been published date. In this review, we critically examine synergy between polysaccharide chemistry microneedle technology enhancing ocular We provide thorough analysis PSMNs, summarizing design principles, fabrication processes, addressed during fabrication, including improving comfort also describe recent advances performance various both research clinical scenarios. Finally, current regulatory frameworks market barriers that are relevant commercial advancement final perspective on area.

Language: Английский

Citations

8
Spatiotemporal diversity and regulation of glycosaminoglycans in cell homeostasis and human disease DOI Creative Commons
Amrita Basu, Neil Patel,

Elijah D. Nicholson

et al.

AJP Cell Physiology, Journal Year: 2022, Volume and Issue: 322(5), P. C849 - C864

Published: March 16, 2022

Glycosaminoglycans (GAGs) are long, linear polysaccharides that ubiquitously expressed on the cell surface and in extracellular matrix of all animal cells. These complex carbohydrates play important roles many cellular processes have been implicated disease states, including cancer, inflammation, genetic disorders. GAGs among most molecules biology with enormous information content extensive structural functional heterogeneity. GAG biosynthesis is a nontemplate-driven process facilitated by large group biosynthetic enzymes extensively characterized over past few decades. Interestingly, expression consequent structure function polysaccharide chains can vary temporally spatially during development under certain pathophysiological conditions, suggesting their assembly tightly regulated Due to key homeostasis disease, there much interest targeting as therapeutic approach. Recent advances genomics analytical techniques pushed field generated new perspectives regulation mammalian glycosylation. This review highlights spatiotemporal diversity mechanisms guiding human disease.

Language: Английский

Citations

27
Small leucine rich proteoglycans: Biology, function and their therapeutic potential in the ocular surface DOI Creative Commons
Tarsis F. Gesteira, Sudhir Kumar Verma, Vivien J. Coulson‐Thomas

et al.

The Ocular Surface, Journal Year: 2023, Volume and Issue: 29, P. 521 - 536

Published: June 22, 2023

Language: Английский

Citations

14