Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)
Published: Oct. 1, 2024
Language: Английский
Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)
Published: Jan. 17, 2025
Hepatocellular carcinoma (HCC) is a global health concern, ranking as the fourth leading cause of cancer-related deaths worldwide. However, role piwi-interacting RNAs (piRNAs) in HCC processes has not been extensively explored. Through small RNA sequencing, our study identified specific piRNA, pir-hsa-216911, which highly expressed cells. This overexpression pir-hsa-216911 promotes cell invasion and inhibits death, particularly pyroptosis. Knocking out led to increased pyroptosis activity, resulting activation caspase-1 GSDMD. Further analysis revealed that targets suppresses TLR4, key gene associated with HCC. In Huh7 line, knockout confirmed its suppressing TLR4/NFκB/NLRP3 pathway by silencing TLR4. significantly inhibited formation xenograft tumor. patients, was tumor samples steatosis, TLR4 expression inhibiting GSDMD activation. introduces new high-expressing piRNA HCC, suppress These findings have significant implications for molecular subtyping potential target cancer therapy.
Language: Английский
Citations
2
Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(10)
Published: Oct. 10, 2023
Abstract Linear ubiquitination is a specific post-translational modification in which ubiquitin linked through M1 residue to form multiple types of polyubiquitin chains on substrates order regulate cellular processes. LUBAC comprised by HOIP, HOIL-1L, and SHARPIN as sole E3 ligase catalyzes the generation linear chains, it simultaneously adjusted deubiquitinases such OTULIN CYLD. Several studies have shown that gene mutation mice accompanied different modalities cell death would develop relative diseases. Cell fundamental physiological process responsible for embryonic development, organ maintenance, immunity response. Therefore, worth speculating mediated signaling pathway participate The literature search was done from core collection electronic databases Web Science, PubMed, Google Scholar using keywords about main regulators pathway. Here, we summarize regulatory mechanism cells with apoptosis, necroptosis, autophagy, pyroptosis, ferroptosis. Intervening might provide novel therapeutic insights various human
Language: Английский
Citations
19
Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 256, P. 155224 - 155224
Published: Feb. 23, 2024
Language: Английский
Citations
6
Cells, Journal Year: 2022, Volume and Issue: 11(12), P. 1882 - 1882
Published: June 9, 2022
The change from viewing noncoding RNA as "junk" in the genome to seeing it a critical epigenetic regulator almost every human condition or disease has forced paradigm shift biomedical and clinical research. Small long transcripts are now routinely evaluated putative diagnostic therapeutic agents. A prominent role for microRNAs central nervous system uncovered promising new candidates dementia-related disorders, treatments which currently remain elusive even percentage of diagnosed patients increases significantly. Cognitive decline is core neurodegenerative process Alzheimer's Disease, Frontotemporal Dementia, Lewy body dementia, vascular Huntington's Creutzfeldt-Jakob disease, significant portion Parkinson's Disease patients. This review will discuss microRNA-associated networks influence these pathologies, including inflammatory viral-mediated pathways (such novel SARS-CoV-2 virus implicated COVID-19), their current status trials.
Language: Английский
Citations
27
Frontiers in Cardiovascular Medicine, Journal Year: 2023, Volume and Issue: 10
Published: June 16, 2023
Cardiovascular disease (CVD) is the leading cause of death worldwide. Pyroptosis a unique kind programmed cell that varies from apoptosis and necrosis morphologically, mechanistically, pathophysiologically. Long non-coding RNAs (LncRNAs) are thought to be promising biomarkers therapeutic targets for diagnosis treatment variety diseases, including cardiovascular disease. Recent research has demonstrated lncRNA-mediated pyroptosis significance in CVD pyroptosis-related lncRNAs may potential prevention specific CVDs such as diabetic cardiomyopathy (DCM), atherosclerosis (AS), myocardial infarction (MI). In this paper, we collected previous on investigated its pathophysiological several illnesses. Interestingly, certain models medications also under control lncRNa-mediated regulation, which aid identification new diagnostic therapy targets. The discovery critical understanding etiology lead novel strategies therapy.
Language: Английский
Citations
11
Molecular Therapy — Nucleic Acids, Journal Year: 2025, Volume and Issue: 36(1), P. 102451 - 102451
Published: Jan. 14, 2025
Language: Английский
Citations
0
Analytical Cellular Pathology, Journal Year: 2025, Volume and Issue: 2025(1)
Published: Jan. 1, 2025
Lung cancer is a highly prevalent and fatal that seriously threatens the safety of people in various regions around world. Difficulty early diagnosis strong drug resistance have always been difficulties treatment lung cancer, so prognosis has focus scientific researchers. This study used genotype-tissue expression (GTEx) genome atlas (TCGA) databases to obtain 477 adenocarcinoma (LUAD) 347 healthy individuals' samples as research subjects divided LUAD patients into low-risk high-risk groups based on prognostic risk scores. Differentially expressed gene (DEG) analysis was performed 25 pyroptosis-related genes obtained from GeneCards MSigDB tissues noncancerous individuals, seven were significantly different among them. Coexpression differential these long noncoding RNAs (lncRNAs) found three lncRNAs (AC012615.1, AC099850.3, AO0001453.2) had significant differences between tissues. We Cox regression least absolute shrinkage sum selection operator (LASSO) construct model for with (pRLs) analyzed value pRLs by Likaplan-Meier curve regression. The results show prediction good ability. In addition, we also studied tumor mutation burden (TMB), immune dysfunction rejection (TIDE), microenvironment scores groups. successfully established pRLs, which provides new insights lncRNA-based strategies.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Feb. 14, 2025
Diabetic nephropathy (DN) is one of the major complications diabetes mellitus. Pyroptosis a type programmed cell death that closely related to development DN, however molecular mechanism pyroptosis in DN still unclear. The aim this study identify pyroptosis-related potential biomarkers and competing endogenous RNA (ceRNA) networks DN. differentially expressed genes (DEPRGs) were identified using R software from Gene Expression Omnibus (GEO) database. In total, 4 significantly upregulated hub DEPRGs (CASP1, TXNIP, IRF9, TRAF3) selected verified by machine learning techniques. Receiver Operating Curve (ROC) assess diagnostic value pivotal DEPRGs. Immune infiltration was analysed CIBERSORT algorithm software. Then, miRNAs (DEmiRNAs) lncRNAs (DElncRNAs) obtained GEO database, respectively. DEPRGs-associated ceRNA network constructed. Finally, rats induced high-sugar high-fat diet combined with an intraperitoneal injection STZ. expression proteins detected rats' kidney tissues Western blotting. constructed validated both clinical samples rat model real-time PCR (qRT-PCR). Our results indicated consisting key might be regulatory axis for
Language: Английский
Citations
0
BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 10, 2025
PANoptosis, a complex programmed cell death (PCD) pathway that includes apoptosis, pyroptosis and necroptosis, is significantly involved in the progression of cancers. Long noncoding RNAs (lncRNAs) play crucial roles PCD. However, predictive value PANoptosis-related lncRNAs (PRlncRNAs) for colon adenocarcinoma (COAD) has not been established. Gene expression data clinical characteristics patients with COAD were obtained from The Cancer Genome Atlas database. Differential analysis Pearson correlation used to identify PRlncRNAs. In addition least absolute shrinkage selection operator, univariate multivariate Cox regression analyses employed obtain PRlncRNAs constructing risk signature. Patients training set, testing set entire stratified into high- low-risk groups further comparison survival prognosis, using median score as cut-off point. Time-dependent receiver operating characteristic curves, nomogram conducted validate signature set. addition, critical pathways, immune infiltration cells, checkpoint-related genes, Tumor Immune Dysfunction Exclusion (TIDE) scores antitumour drugs compared between two Correlations ferroptosis, cuproptosis, disulfidptosis PRlncRNA evaluated. Finally, competitive endogenous RNA (ceRNA) network was established, enrichment predicted mRNAs performed Ontology (GO) analysis. Kaplan-Meier plotter database an external confirm accuracy predicting patient prognosis. Additionally, small interfering (siRNA), counting kit- 8 assay, colony formation quantitative polymerase chain reaction (qPCR) apoptosis assay investigate AP003555.1 cancer. A comprising four (LINC01133, FOXD3-AS1, AP001066.1, AP003555.1) developed predict prognosis COAD. Kaplan‒Meier curves demonstrated significant differences across three sets. Multivariate confirmed independent prognostic factor nomogram, calibration indicated strong confidence Using CIBERSORT algorithm gene analysis, variations infiltrating cells processes observed groups. Furthermore, TIDE suggested high-risk group exhibited lower immunotherapy escape better outcomes than group. Distinct responses various we constructed ceRNA based on PRlncRNAs, GO revealed different functions. results who high levels LINC01133 FOXD3-AS1 experienced shorter overall those low these lncRNAs. Specifically, terms functionality, found be highly expressed cancer tissue promoted viability proliferation while suppressing cells. We identified novel consisting which indicator This potentially relevant could serve therapeutic target
Language: Английский
Citations
0
Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown
Published: April 18, 2025
Language: Английский
Citations
0