Impact of periodontitis on circulating cell-free DNA levels as a measure of cardiovascular disease DOI Creative Commons
Gaetano Isola, Alessandro Polizzi, Marco Mascitti

et al.

Clinical Oral Investigations, Journal Year: 2023, Volume and Issue: 27(11), P. 6855 - 6863

Published: Oct. 9, 2023

Abstract Objectives The present study aims to assess the serum circulating cell-free (cfDNA) concentrations in patients with periodontitis and cardiovascular disease (CVD) evaluate impact of on cfDNA levels confounding factors that might mediated possible relationship. Materials methods Healthy controls ( n =30) CVD =31), + were enrolled study. All subjects underwent regular periodontal examination blood sampling evaluation. analysis plasma was performed using a dsDNA Assay Kit. Results In comparison healthy patients, periodontitis+CVD exhibited significantly higher expression p <0.05). There positive correlation among clinical attachment loss (CAL) =0.019), high sensitivity C-reactive protein (hs-CRP) =0.027), inflamed surface area (PISA) =0.003). Furthermore, multivariate regression evidenced PISA <0.001), hs-CRP =0.014), full-mouth bleeding score (FMBS) =0.004) significant predictors concentrations. Conclusions results highlighted group showed patients. Moreover, extent correlated increased represented predictor Clinical relevance Unbalanced have been indicated represent risk endothelial dysfunction. Periodontitis expression; moreover, predicted concentrations, suggesting potential developing

Language: Английский

Extracellular host DNA contributes to pathogenic biofilm formation during periodontitis DOI Creative Commons

Mariana Slobodianyk-Kolomoiets,

Svitlana Khlebas,

И.П. Мазур

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 8, 2024

Introduction Periodontal diseases are known to be associated with polymicrobial biofilms and inflammasome activation. A deeper understanding of the subgingival cytological (micro) landscape, role extracellular DNA (eDNA) during periodontitis, contribution host immune eDNA persistence, may improve our mechanisms underlaying severe forms periodontitis. Methods In this work, biolfilms developing on biologically neutral polyethylene terephthalate films placed in gingival cavities patients chronic periodontitis were investigated by confocal laser scanning microscopy (CLSM). This allowed examination realistic landscapes visualization polymeric substances (EPS) including amyloids, total proteins, carbohydrates eDNA, as well comparison several single-strain vitro model produced oral pathogens such Klebsiella pneumoniae , Pseudomonas aeruginosa Staphylococcus aureus Streptococcus gordonii S. sanguinis mitis . Fluorescence situ hybridization (FISH) analysis was also used identify derived from eubacteria, streptococci members Bacteroides–Porphyromonas–Prevotella (BPP) group Results Analysis biofilm EPS revealed low levels amyloids high which appears main matrix component. However, bacterial contributed less than a third observed, suggesting that host-derived released neutrophil traps more importance development causing Discussion immunocompetent cells activated at onset therefore major driver persistence pathogenesis.

Language: Английский

Citations

4
Circulating Microbiota in Cardiometabolic Disease DOI Creative Commons
Keiichi Sumida,

Zhongji Han,

Chi‐Yang Chiu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: May 3, 2022

The rapid expansion of microbiota research has significantly advanced our understanding the complex interactions between gut and cardiovascular, metabolic, renal system regulation. Low-grade chronic inflammation long been implicated as one key mechanisms underlying cardiometabolic disease risk progression, even before insights provided by in past decade. Microbial translocation into bloodstream can occur via different routes, including through oral and/or intestinal mucosa, may contribute to disease. Among several gut-derived products identifiable systemic circulation, bacterial endotoxins metabolites have extensively studied, however recent advances microbial DNA sequencing further allowed us identify highly diverse communities microorganisms from an -omics standpoint, which is termed “circulating microbiota.” While detecting was historically considered indication infection, evidence on circulating continually accumulating various patient populations without clinical signs infection otherwise healthy individuals. Moreover, both quantitative compositional alterations recently pathogenesis inflammatory conditions, potentially their immunostimulatory, atherogenic, cardiotoxic properties. In this mini review, we aim provide characteristics roles diseases, such type 2 diabetes, cardiovascular disease, kidney with highlights emerging findings patients end-stage undergoing hemodialysis.

Language: Английский

Citations

19
Molecular Methodologies for Improved Polymicrobial Sepsis Diagnosis DOI Open Access

Mariam Doualeh,

Matthew S. Payne, Edward Litton

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 4484 - 4484

Published: April 19, 2022

Polymicrobial sepsis is associated with worse patient outcomes than monomicrobial sepsis. Routinely used culture-dependent microbiological diagnostic techniques have low sensitivity, often leading to missed identification of all causative organisms. To overcome these limitations, culture-independent methods incorporating advanced molecular technologies recently been explored. However, contamination, assay inhibition and interference from host DNA are issues that must be addressed before can relied on for routine clinical use. While the component complex host-pathogen interplay well described, less known about pathogen's role, including pathogen-pathogen interactions in polymicrobial This review highlights significance addresses how promising alternative microbiology improved detect infections. It also discusses application shotgun metagenomics uncover pathogen/pathogen cases their potential role course this condition.

Language: Английский

Citations

18
Oral Normality Alterations and the Impact on Pneumonia, Sepsis, and Bloodstream Infections in Critically Ill Patients: A Prospective Cohort Study DOI

Camila Silveira,

C. Vieira,

Alexandre B. Cavalcanti

et al.

Published: Jan. 1, 2025

Objective: To determine the prevalence of oral alterations among critical patients within first 48 hours ICU admission, and their association with hospital-acquired pneumonia, bloodstream infections, sepsis.Methods: This prospective cohort study was conducted from March 2018 to December 2021 in a Brazilian neurology ICU. A single dental surgeon assessed conditions follow-up until hospital discharge. Logistic regression analyzed between outcomes (hospital-acquired sepsis), adjusting for confounders such as comorbidities, corticosteroid use, age, dysphagia, tracheal intubation.Results: We enrolled 248 (55.6% male, mean age 67.2 years). total 242 (97.6%) had abnormalities. The most common were visible plaque (61.7%), gingival inflammation (60.9%), five or more missing teeth (49.2%). Carious linked pneumonia (OR 1.10; 95% CI 1.00-1.20; p = 0.047). Destroyed 1.12; 1.02-1.23; 0.02) 1.08; 1.00-1.17; 0.04) associated infections. No factors sepsis.Conclusion: Most exhibited after admission. may increase risk, while destroyed raise infection risk.

Language: Английский

Citations

0
Post‐Treatment Apical Periodontitis: Endodontic Infection Contributes to Circulating Microbial DNA DOI Open Access
Yanglin Zeng, Liu Wang, Mudan Wang

et al.

Australian Endodontic Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

ABSTRACT This study investigated the impact of post‐treatment apical periodontitis on microbial DNA in patients' blood circulation. Samples from root apex, periapical lesion and peripheral 11 patients, along with nine healthy controls, were analysed using next‐generation sequencing. The composition, diversity linear discriminant analysis effect size analysed. SourceTracker was applied to trace microorganisms blood. Proteobacteria dominated lesions, while Actinobacteria prevailed apex. Patients exhibited higher but reduced α‐diversity apex compared other sites. Moreover, Bacteroides , as well Aliihoeflea Oceanicaulis Comamonas Shigella Acinetobacter showed increased relative abundance patients. Additionally, contributed 26% 76% for bacteria. These findings suggested unique translocation

Language: Английский

Citations

0
Bacterial translocation signatures and subgingival microbiome in individuals with periodontitis DOI

Marcelo Britos,

Marcela Hernández, Alejandra Fernández

et al.

Clinical Oral Investigations, Journal Year: 2025, Volume and Issue: 29(6)

Published: May 7, 2025

Language: Английский

Citations

0
High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease DOI Creative Commons

David C. Emery,

Maria Davies,

Tanya L. Cerajewska

et al.

Frontiers in Aging Neuroscience, Journal Year: 2022, Volume and Issue: 14

Published: Dec. 9, 2022

Introduction Alzheimer’s (AD) and Parkinson’s disease (PD) are neurodegenerative conditions characterized by incremental deposition of β-amyloid (Aβ) α-synuclein in AD PD brain, respectively, relatively conserved patterns. Both associated with neuroinflammation, a proposed microbial component for initiation and/or progression. Notably, Aβ have been shown to possess antimicrobial properties. There is evidence bacterial presence within the including oral pathobiont Porphyromonas gingivalis , cognitive impairment brain pathology being linked periodontal (gum) gut dysbiosis. Methods Here, we use high resolution 16S rRNA PCR-based Next Generation Sequencing (16SNGS) characterize composition areas early, intermediate late-stage diseases. Results discussion This study reveals widespread bacteria pathology, distinctly different profiles blood brain. Brain area were overall somewhat similar, predominantly oral, some subgingival oronasal origin, comparable However, early development, such as locus coeruleus, substantially DNA content compared affected later etiology.

Language: Английский

Citations

15
Another Look at the Contribution of Oral Microbiota to the Pathogenesis of Rheumatoid Arthritis: A Narrative Review DOI Creative Commons
Jean‐Marie Berthelot, Octave Nadile Bandiaky, Benoît Le Goff

et al.

Microorganisms, Journal Year: 2021, Volume and Issue: 10(1), P. 59 - 59

Published: Dec. 28, 2021

Although autoimmunity contributes to rheumatoid arthritis (RA), several lines of evidence challenge the dogma that it is mainly an autoimmune disorder. As RA-associated human leukocyte antigens shape microbiomes and increase risk dysbiosis in mucosae, RA might rather be induced by epigenetic changes long-lived synovial presenting cells, stressed excessive translocations into joints bacteria from poorly cultivable gut, lung, or oral microbiota (in same way as more pathogenic can lead “reactive arthritis”). This narrative review (i) lists supporting this scenario, including identification DNA gut synovium (but also healthy synovia), possibility translocation through blood, mucosae joints, microbiota, either directly cavity following permeability worsened migration within such Porphyromonas gingivalis; (ii) suggests other methodologies for future works than cross-sectional studies periodontal cohorts patients with versus controls, namely, longitudinal oral, combined transcriptomic analyses immune cells individual at RA, overt before, during, flares RA.

Language: Английский

Citations

19
Periodontal disease in cats under primary veterinary care in the UK: frequency and risk factors DOI
Dan G. O’Neill,

Alyx Blenkarn,

Dave C. Brodbelt

et al.

Journal of Feline Medicine and Surgery, Journal Year: 2023, Volume and Issue: 25(3)

Published: March 1, 2023

Objectives Periodontal disease is a multifactorial inflammatory that can have major welfare implications in cats. This study aimed to report the frequency and demographic risk factors of periodontal explore associations with common comorbid disorders cats UK. Methods The included random sample 18,249 obtained from 1,255,130 under primary care during 2019 clinics participating VetCompass programme. All recorded were extracted reported. Risk factor disorder analysis used multivariable logistic regression modelling. Results had 1-year period prevalence 15.2% (95% confidence interval [CI] 14.72–15.76). Breeds highest Siamese (18.7%, 95% CI 12.24–26.72) Maine Coon (16.7%, 11.37–23.18). median age (9.47 years, interquartile range [IQR] 5.96–12.97) was higher than for without (4.94 IQR 1.95–9.51; P <0.001). Increasing adult body weight, increasing sex–neuter status significantly associated rising odds disease. Cats count per individual cat (3, 2–4, 1–14) (1, 0–2, 0–15; 1.79 times 1.62–1.99, <0.001) diagnosis at least one Conclusions relevance most specific confirmed as leading health issue Ageing identified strongest predictor cats, suggesting potential gains emphasis on dental they age. offers evidence close link between reduced overall

Language: Английский

Citations

7
A Low-Cost Protocol Using the Adjunctive Action of Povidone–Iodine Irrigations and Sodium Hypochlorite Rinsing Solution in Step 2 of Periodontal Therapy for Patients with Stage III–IV Periodontitis: A Single-Blind, Randomized Controlled Trial DOI Creative Commons

Georgios Kardaras,

Ruxandra Christodorescu, Marius Boariu

et al.

Dentistry Journal, Journal Year: 2024, Volume and Issue: 12(5), P. 144 - 144

Published: May 15, 2024

In severe stages of periodontitis, conventional periodontal therapy and maintenance care are usually insufficient due to the viral bacterial etiology; thus, a mechanical approach alone may not be sufficient eliminate substantial portion subgingival pathogens, especially in deep sites. Background Objectives: This single-blind, randomized clinical trial aimed compare microbiological efficacy low-cost protocol using povidone–iodine sodium hypochlorite formulations as adjuncts non-surgical for patients with stage IV periodontitis when compared chlorhexidine, most commonly employed substance date antimicrobial regimens therapy. Materials Methods: Forty-five were randomly divided into two groups: control (subgingival instrumentation, chlorhexidine-assisted) test (antiviral medication, instrumentation povidone–iodine, rinsing solution, antibiotics). Clinical measurements analyses performed at baseline after three months. Results: After months, notable differences found detection scores Porphyromonas gingivalis (a significant reduction frequency was observed (p = 0.021)), there reductions group Tannerella forsythia Treponema denticola, showing undetectable levels < 0.0001 both). group, pocket probing depth median value reduced significantly 0.0005); similarly, bleeding on showed marked decrease 0.0001). However, changes attachment loss full-mouth plaque score statistically significant. Conclusions: Using proposed protocol, improvements parameters obtained current recommendations.

Language: Английский

Citations

2